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Abstracts. PHYTOPHARM 2017
(PGRs), and on variant without PGRs (control), as follows: A - control, B - 0.1 mg/l BA and 2 mg/l NAA, C -0.5 mg/l BA and 2 mg/l NAA, D - 2mg/l BA and 0.5 mg/l NAA, E - 2 mg/l BA and 1 mg/l NAA, F - 2 mg/l BA and 2 mg/l NAA, G - 3 mg/l BA and 1 mg/l NAA, under constant artificial light. The cultures were maintained for 30 days (3 series). Quantification of schisandra lignans was performed using the LC-DAD and LC-DAD-MS methods [2, 5].
The main compounds were: schisandrin (max. 176.31 mg/100 g DW, variant E), gomisin A (max. 49.55 mg/100 g DW, variant G) and deoxyschisandrin (max. 34.02 mg/100 g DW, variant G). The highest total content of lignans (490.25 mg/100 g DW) was obtained
in extracts from biomass of cultures cultivated on MS medium variant G.
The established cultures of S. chinensis cv. Sadova No. 1, can be proposed as a richer source of bioactive lignans. The estimated total lignan content was about 2-times higher than in microshoot cultures of S. chinensis cultivated in the same conditions.
References:
1. Szopa A. et al. 2017. Phytochem Rev. 16:195-218.
2. Szopa A. et al. 2016. Appl. Microbiol. Biotechnol. 100: 3965-3977.
3. Szopa A. et al. 2017. J. Biotechnol. 14:11-17.
4. Murashige T, Skoog F. 1962. Physiol Plant. 15:473-449.
5. Zhang H. et al. 2009. Food Chem. 115:735-739.
THE AGITATED MICROSHOOT CULTURES OF RED AND PURPLE ARONIAS - A POTENTIAL SOURCE OF BIOACTIVE FLAVONOIDS FOR PHYTOTHERAPY
© Agnieszka Szopa, Pawef Kubica, Halina Ekiert
Chair and Department of Pharmaceutical Botany, Jagiellonian University, Collegium Medicum, Krakow, Poland
Aronia melanocarpa (black chokeberry), plant species of North American origin has the strong position as a medicinal and culinary species in Europe. Fruits of black aronia are rich in anthocyanins and phenolic acids. Leaves contain high amounts of flavonoids [1, 2]. The all mentioned groups of the metabolites with polyphenol structures are valuable antioxidants in phytotherapy and also in cosmetology. The less popular in Europe aronia species of North American origin are A. arbutifolia (red aronia) and the hybrid A. x prunifolia (purple aronia). The fruits and leaves of these plants contain also the same groups of polyphenols as black aronia (2). The ability for high phenolic acids production of cells from shoot cultures of red and purple aronias cultured in agitated culture system was documented by us earlier. The aim of the presented study was the investigation on accumulation of flavonoids in the same in vitro culture system.
Agitated shoot cultures of red and purple aronias were maintained on 4 variants of Murashige-Skoog medium (3) with different concentrations of plant growth regulators; BA and NAA (from 0.5 to 3 mg/l). In the methanolic extracts from microshoots 11 flavonoids (aglycones and glycosides) were estimated by LC-DAD method (4).
The compounds produced by cells from in vitro cultures were: quercetin, quercitrin and rutin. The amounts of individual metabolites and their total contents were dependent on medium variants. The main metabolites in biomass of both plants were glycoside of quercetin - quercitrin (max. 41.14 and 47.86 mg/100 g DW, respectively).The highest total amounts of metabolites were 65.26 and 78.34 mg/100 g DW (red and purple aronia respectively).
The obtained amounts of quercitrin are interesting from practical point of view. We expect that, the next step of experiments with addition of flavonoids precursors into the culture media should result by even higher amounts of investigated antioxidants.
References:
1. Kokotkiewicz A. et al. 2010. J. Med. Food. 13:255-269.
2. Szopa A. et al. 2017. Eur.Food Res. Technol., DOI: 10.1007/s00217-017-2872-8.
3. Murashige T, Skoog F. 1962. Physiol. Plant. 15:473449.
4. Ellnain-Wojtaszek M, Zgorka GJ. 1999. Liq. Chrom. Rel. Tech., 22:1457-1471.
ANALYSIS OF THE ESSENTIAL OIL OF THE CATNIP (NEPETA CATARIA L.)
© Terninko I.I.
Saint-Petersburg State Chemical-Pharmaceutical Academy, Saint-Petersburg, Russia.
In traditional medicine Nepeta cataria L. was used as immunomodulatory and antioxidant activities have been a sedative, spasmolytic, antidepressive. and to relieve reported [1-3]. The medicinal properties of Nepeta cataria gastrointestinal and respiratory disorders [4]. Antimicrobial, L. are usually attributed to their essential oils.
Obzory po kliniceskoj farmacologii i lekarstvennoj terapii [Reviews of clinical pharmacology and drug therapy] vol. 15/2017/suppLement 1
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