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analytical, then in preparative scale. Sixty one-minute fractions were collected and analyzed by mean of HPLC. After injection of 500 mg of the oily extract 2,63 mg of lucidafuranocoumarin A and 8,82 mg of bergamottin was obtained in a single run in less than 50 minutes. The structure of compounds was determined by NMR and MS (UHPLC-TOF-MS) analyses.
Acknowledgements: The work was financed from grant No 4/POLTUR-1/2016
References:
1. Skalicka-Wozniak K, tos R, Gtowniak K, Malm A. 2008. Acta Chromatogr. 20(1):119-133.
2. Skalicka-Wozniak K, Mroczek T, Garrard I, Gtowniak K. 2012. J. Sep. Sci. 35(7): 790-797.
3. Skalicka-Wozniak K, tos R, Gtowniak K, Malm A. 2010. Chem. Biodivers. 7(11): 2748-2754.
EFFECTS OF LITSEA JAPONICA FRUIT EXTRACT AND ITS CONSTITUENTS ON HEPATITIS E VIRUS REPLICATION
© Gayong Park, Yoon-Jae Song
Department of Life Science, Gachon University, Seongnam-Si, Gyeonggi-Do, Republic of Korea
Hepatitis E virus (HEV) is the major etiological agent of acute viral hepatitis and a member of the genus Hepevirus of the family Hepeviridae [1]. To identify a novel antiviral candidate for the HEV-caused hepatitis E, crude ethanol extract of medicinal plants were screened for the potential inhibitory activity on HEV replication using a genotype 3 HEV-luciferase replicon [2]. Seventy percent ethanol extract of Litsea japonica fruit (LJE) reduced HEV replication by 67.2% without exhibiting any significant adverse effects on the viability of human hepatocarcinoma Huh-7.5 cell line. To determine the solvent fraction that inhibits HEV replication, activity-
guided fractionation of LJE was further performed. Among the solvent fractions tested, the ethyl acetate (EtOAc) fraction of LJE reduced HEV replication by 71% in vitro. Thus, the EtOAc fraction of LJE is a good source of novel drug candidates for treatment of the HEV-caused hepatitis E.
References:
1. Nan Y, Zhang YJ. 2016. Front Microbiol. 7:1419.
2. Pudupakam RS, Kenney SP, Cordoba L, Huang YW, Dryman BA, Leroith T, Pierson FW, Meng XJ. 2011. J Virol. 85:10031-10040.
ACUTE AND SUB-CHRONIC (28 DAYS) REPEATED ORAL TOXICITY TEST OF DRIED EXTRACT OF SAMBILOTO HERBS (ANDROGRAPHIS PANICULATA NEES.) AND MAHOGANY SEEDS (SWIETENIA MAHAGONI JACQ.) COMBINATION
© Sukardiman, Ricko Hartanto, Lusiana Arifianti, Herra Studiawan, Rakhmawati
Departmen of Pharmacognosy and Phytochemistry, Faculty of Pharmacy, Airlangga University, Surabaya, Indonesia
The present study was carried out to evaluate the safety of dried extract of sambiloto herbs (Andrographis paniculata Nees.) and mahogany seeds (Swietenia mahagoni Jacq.) combination.
Sambiloto herbs (Andrographis paniculata Nees.) and mahogany seeds (Swietenia mahagoni Jacq.) was extracted by ethanol 96 %. Extraction results was continued to dried using avicell and cab-o-sil. Dried extract was continued to acute toxicity by using a fixed dose method and sub-chronic toxicity was performed according to the OECD guideline. In an acute toxicity study, a single dose of 1000, 2000, 4000, 8000 and 16000 mg/kg bw of its dried extract combination was administered p.o (orally) to healthy female mice following a sighting study. The animals were observed for mortality and clinical signs for 24 hour and then daily for 14 days. In the sub-chronic toxicity study, the extract was administered orally at doses of 250, 500 and
1000 mg/kg bw /day to healthy Wistar rats. The animals were given by dried extract of combination and once daily for 28 days, the administrations were stopped on the 28th day, while for the satellite group still observed until 14 days during the post observation period for assessment of reversibility, persistence or delayed occurrence of toxicity. At the end of the observation, all animals were autopsied and observed in parameters such as blood biochemical parameters, hematology and relative vital organ weight.
In the acute toxicity test, oral administration of 1000, 2000, 4000, 8.000 and 16.000 mg/kg bw produced neither mortality or changes in behavior or any other physiological activities and indicated that LD50 of dried extract combination was greater than 16.000 mg/kg bw in Wistar Rats. In sub-chronic 28-days repeated dose oral toxicity study, its combination dried exctract of 250 mg/ kg bw, 500 mg/kg bw 1000 mg/kg bw in male and female
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rats generally showed no significantly differences in parameters abnormalities of organ function, behavior and motor activity, blood biochemical parameters, hematology parameters, and did not produce mortality in treated groups compared to control group. Increasing dosage combination showed significantly increasing SGOT in male Wistar rats and MCHC in female Wistar
rats. The dried exctract combination also significantly decreased basophils in male Wistar rats and eosinophils in female Wistar rats.
Based on the finding of this study, the no-observed-adverse-effect-level (NOAEL) of dried extract combination in Wistar rats, following oral administration for 28 days was found to be more than 1000 mg/kg bw.
ISOLATION OF INDIVIDUAL FLAVONOIDS FROM SOLIDAGO CANADENSIS L.
© Suloev I.S., Luzhanin V.G.
St. Petersburg State Chemical-Pharmaceutical Academy, St. Petersburg, Russia
The advantages of herbal medicines in comparison with synthetic analogues are mainly in the complex effect and the possibility of long-term use without significant side effects. In addition, some medicines that are especially valuable for medicine are still receive only from plants (cardiac glycosides, strychnine, photosensitizing drugs).
The purpose of this work is to conduct phytochemical analysis and isolation of individual substances from the grass of Solidago canadensis L. The known diuretic, anti-inflammatory and antibacterial activity of the summary extracts of Solidago canadensis gives the possibility of the creation of a new medicinal drug for urological practice [1, 2].
In the course of the work, the following tasks are planned: harvesting the target plant raw materials and obtaining total extracts, isolating the individual substances from the total extracts, establishing and confirming the structure of the individual compounds and preparative isolation of the targeted individual substances.
At the moment, harvesting and grinding of the Solidago canadensis grass has been carried out, the optimal solvents for vacuum chromatography (methanol) have been selected, and total extractions
have been obtained. Preliminary tests were carried out: paper chromatography in 2 solvent systems: 15% acetic acid and BAW (buthanol-1, acetic acid and water) -(4: 1: 2), and TLC analysis in BAW and dichloromethane-methanol systems (5: 1). According to the obtained data, substances that give fluorescence in UV radiation were found in the analyzed fractions. The chemical structure of substances will be determined.
Also, HPLC analysis of these fractions was carried out. Four fractions were obtained from the ethanol extract of the Solidago canadensis: hexanoic, dichloromethane, ethyl acetate, and methanol. According to the data obtained the most promising from the point of view of the isolation of individual flavonoids is the methanol fraction. In the methanol fraction there are 5 compounds belonging to the class of flavonoids. In the future, it is planned to separate and preparatively isolate these substances.
References:
1. Jiang T, Huang BK, Qin LP. 2006. J Chin Integr Med.
4(4):430-435.
2. Mishra D, Joshi S, Bisht G, Pilkhwal S. 2010. J Basic
Clin Pharm. 1(3):187-90.
BIOACTIVE SCHISANDRA LIGNANS IN MICROSHOOT CULTURES OF SCHISANDRA CHINENSIS CV. SADOVA NO. 1 -PERSPECTIVE FOR PHYTOTHERAPHY
© Agnieszka Szopa, Marta Klimek, Halina Ekiert
Chair and Department of Pharmaceutical Botany, Jagiellonian University, Collegium Medicum, Krakow, Poland
Schisandra chinensis (Turcz.) Baill. - Chinese magnolia vine it's pharmacopoeial plant species also in Europe with well-established pharmaceutical activity. Schisandrae chinensis fructus, shows adaptogenic, hepatoprotective, antioxidant and anticancer activity. Biological activity of the plant is attributed mostly to dibenzocyclooctadiene lignans, called the schisandra lignans [1]. Shoot-differentiating callus cultures of
S. chinensis established in our laboratory were capable of accumulation of high amounts of lignans [2, 3]. The aim of the present study was to investigate the accumulation of these compounds in in vitro cultures of Ukrainian cultivar - S. chinensis cv. Sadova No. 1.
Agar microshoot cultures were maintained on six variants of Murashige and Skoog (MS) medium [4], differing in concentrations of the plant growth regulators
Obzory po kliniceskoj farmacologii i lekarstvennoj terapii [Reviews of clinical pharmacology and drug therapy]
vol. 15/2017/suppLement 1
