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Abstracts. PHYTOPHARM 2017
analytical, then in preparative scale. Sixty one-minute fractions were collected and analyzed by mean of HPLC. After injection of 500 mg of the oily extract 2,63 mg of lucidafuranocoumarin A and 8,82 mg of bergamottin was obtained in a single run in less than 50 minutes. The structure of compounds was determined by NMR and MS (UHPLC-TOF-MS) analyses.
Acknowledgements: The work was financed from grant No 4/POLTUR-1/2016
References:
1. Skalicka-Wozniak K, tos R, Gtowniak K, Malm A. 2008. Acta Chromatogr. 20(1):119-133.
2. Skalicka-Wozniak K, Mroczek T, Garrard I, Gtowniak K. 2012. J. Sep. Sci. 35(7): 790-797.
3. Skalicka-Wozniak K, tos R, Gtowniak K, Malm A. 2010. Chem. Biodivers. 7(11): 2748-2754.
EFFECTS OF LITSEA JAPONICA FRUIT EXTRACT AND ITS CONSTITUENTS ON HEPATITIS E VIRUS REPLICATION
© Gayong Park, Yoon-Jae Song
Department of Life Science, Gachon University, Seongnam-Si, Gyeonggi-Do, Republic of Korea
Hepatitis E virus (HEV) is the major etiological agent of acute viral hepatitis and a member of the genus Hepevirus of the family Hepeviridae [1]. To identify a novel antiviral candidate for the HEV-caused hepatitis E, crude ethanol extract of medicinal plants were screened for the potential inhibitory activity on HEV replication using a genotype 3 HEV-luciferase replicon [2]. Seventy percent ethanol extract of Litsea japonica fruit (LJE) reduced HEV replication by 67.2% without exhibiting any significant adverse effects on the viability of human hepatocarcinoma Huh-7.5 cell line. To determine the solvent fraction that inhibits HEV replication, activity-
guided fractionation of LJE was further performed. Among the solvent fractions tested, the ethyl acetate (EtOAc) fraction of LJE reduced HEV replication by 71% in vitro. Thus, the EtOAc fraction of LJE is a good source of novel drug candidates for treatment of the HEV-caused hepatitis E.
References:
1. Nan Y, Zhang YJ. 2016. Front Microbiol. 7:1419.
2. Pudupakam RS, Kenney SP, Cordoba L, Huang YW, Dryman BA, Leroith T, Pierson FW, Meng XJ. 2011. J Virol. 85:10031-10040.
ACUTE AND SUB-CHRONIC (28 DAYS) REPEATED ORAL TOXICITY TEST OF DRIED EXTRACT OF SAMBILOTO HERBS (ANDROGRAPHIS PANICULATA NEES.) AND MAHOGANY SEEDS (SWIETENIA MAHAGONI JACQ.) COMBINATION
© Sukardiman, Ricko Hartanto, Lusiana Arifianti, Herra Studiawan, Rakhmawati
Departmen of Pharmacognosy and Phytochemistry, Faculty of Pharmacy, Airlangga University, Surabaya, Indonesia
The present study was carried out to evaluate the safety of dried extract of sambiloto herbs (Andrographis paniculata Nees.) and mahogany seeds (Swietenia mahagoni Jacq.) combination.
Sambiloto herbs (Andrographis paniculata Nees.) and mahogany seeds (Swietenia mahagoni Jacq.) was extracted by ethanol 96 %. Extraction results was continued to dried using avicell and cab-o-sil. Dried extract was continued to acute toxicity by using a fixed dose method and sub-chronic toxicity was performed according to the OECD guideline. In an acute toxicity study, a single dose of 1000, 2000, 4000, 8000 and 16000 mg/kg bw of its dried extract combination was administered p.o (orally) to healthy female mice following a sighting study. The animals were observed for mortality and clinical signs for 24 hour and then daily for 14 days. In the sub-chronic toxicity study, the extract was administered orally at doses of 250, 500 and
1000 mg/kg bw /day to healthy Wistar rats. The animals were given by dried extract of combination and once daily for 28 days, the administrations were stopped on the 28th day, while for the satellite group still observed until 14 days during the post observation period for assessment of reversibility, persistence or delayed occurrence of toxicity. At the end of the observation, all animals were autopsied and observed in parameters such as blood biochemical parameters, hematology and relative vital organ weight.
In the acute toxicity test, oral administration of 1000, 2000, 4000, 8.000 and 16.000 mg/kg bw produced neither mortality or changes in behavior or any other physiological activities and indicated that LD50 of dried extract combination was greater than 16.000 mg/kg bw in Wistar Rats. In sub-chronic 28-days repeated dose oral toxicity study, its combination dried exctract of 250 mg/ kg bw, 500 mg/kg bw 1000 mg/kg bw in male and female
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