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Scientific Messenger of Lviv National University of Veterinary Medicine and Biotechnologies
ISSN 2518-7554 print ISSN 2518-1327 online
doi: 10.15421/nvlvet8351 http://nvlvet.com.ua/
UDC 636.09:616.98:579.62
Etiological structure of bacteriosis of animals in the Dnipropetrovsk region for 20142016
V.V. Glebenyuk1, I.V. Borovik2, T.V. Kuchuk1, O.O. Litvinenko1
'Dnipro State Agrarian and Economic University, Dnipro, Ukraine 2Dnipropetrovsk Regional State Laboratory of Veterinary Medicine, Dnipro, Ukraine
Article info
Received 30.0'.20'8 Received in revised form 28.02.2018 Accepted 05.03.2018
Dnipro State Agrarian and Economic University, Efremova Str., 25, Dnipro, 49600, Ukraine. Tel.: +38-0562-68-54-36 E-mail: hlebeniuk.v.v@dsau.dp.ua
Dnipropetrovsk Regional State Laboratory of Veterinary Medicine, Oleksandra Polya Ave., 48, Dnipro, 49000, Ukraine.
Glebenyuk, V. V., Borovik, I.V., Kuchuk, T.V., & Litvinenko, O.O. (2018). Etiological structure of bacteriosis of animals in the Dnipropetrovsk region for 2014-2016. Scientific Messenger of Lviv National University of Veterinary Medicine and Biotechnologies. 20(83), 260-263. doi: 10.15421/nvlvet8351
The epizootic situation with regard to some infectious diseases in the Dnipropetrovsk region remains as complicated and strained. Timely and accurate diagnosis determines the effectiveness of the realized preventive measures of infectious diseases and establishes the peculiarities and changes of the epizootic situation in certain areas. The laboratory examinations, including the bacteriological method, have the crucial importance for the correct assessment of prevention and antiepizootic measures. Objective of this review: was to determine the etiological .structure of infectious diseases in the Dnipropetrovsk region for 2014-2016, based on the results of bacteriological examinations. As the material for the examinations were the data of the veterinary reports of the State district veterinary medicine laboratories of the Dnipropetrovsk region and the results of our own examinations conducted on the basis of the Dnipropetrovsk Regional State Laboratory of the Veterinary Medicine (DRSLVM). As of 2014 year, in the Dnipropetrovsk region, the livestock capita in all categories of farms was: horned cattle - 130.527, small cattle - 44.739, pigs - 520.801, horses - 1.781, poultries - 15.342,087, including chickens - 14.816.157, waterfowl - 525.930. During 2014-2016 by means of laboratories of veterinary medicine in the Dnipropetrovsk region have been conducted 18.474 examinations on the infectious diseases of the animals. 142 positive results were received in total. According to the results of the bacteriological examinations in the Dnipropetrovsk region, for the years 2014-2016 were registered diplococcosis, malignant edema, pasteurellosis, colibacteriosis, salmonel-losis, pullorosis, staphylococcosis, streptococcosis and swine erysipelas, poultry pseudomonosis, aer-omonosis offish, parafoul brood (caused by Bacillus paraalvei) and proteose of bees. 4.472 examinations were conducted on colibacteriosis and 78 positive results were obtained. 21 cultures were obtainedfrom the pigs, from poultries - 44 cultures, from embryos - 6 cultures, from small cattle - 3 cultures, from fur animals - 2 cultures, from dogs - 2 cultures. Cultures of Escherichia coli, isolated from pigs are represented by the serological variants: O139, O26, O9, O78, O1, O8 and atypical variants, and from poultries - O115, O119, O78, O15, O86, K88 AB and atypical variants. For salmonella, 7.578 examinations were conducted and 16 positive results were obtained. Among the diseases of bees, laboratory tests have been recorded parafoul brood and proteose of bees. Conclusion: causative agents of 13 infectious animal diseases have been identified in the Dnipropetrovsk region by means of bacteriological investigations. Most commonly isolated pathogens of intestinal infections (colibacteriosis and salmonellosis).
Key words: Dnipropetrovsk region, bacteriosis, animals, colibacteriosis, salmonellosis, bacteriological examinations.
ЕттлоНчна структура 6aKTepio3iB тварин у Дшпропетровськш облает за 2014-2016 роки
В.В. Глебенюк1, 1.В. Боровик2, Т.В. Кучук1, О.О. Литвиненко1
'Днтровський державний аграрно-eKOHOMiHmü yHieepcumem, м. Днтро, Укра'ша 2Днтропетровська регiональна державна лабораторiя ветеринарно'1 медицини, м. Днтро, Украта
Етзоотична ситуащя щодо деяких тфекцшних хвороб на Днтропетровщит залишаеться складною i напруженою. Своечасна й точна дiагностика визначае ефективтсть реалiзованих заходiв профилактики тфекцшних хвороб та встановлюе особливостi i змти етзоотичног ситуацп на певних територiях. Лабораторт до^дження, в тому чиmi бактерiологiчний метод, мають вирi-шальне значення для правильног оцтки профтактичних i протиешзоотичних заходiв. Метою нашог роботи було визначити за результатами бактерiологiчних до^джень етiологiчну структуру тфекцшних хвороб у Днтропетровськт областi за 2014-2016роки. Матерiалом для до^джень були дан ветеринарног звiтностi державних районних лабораторш ветеринарног медици-ни Днтропетровськог областi та результати власних до^джень, проведен на базi Днтропетровськог регюнальног державног лабораторп ветеринарног медицини (ДРДЛВМ). Станом на 2014рт у Днтропетровсьтй областi поголiв 'я тварин по в^х катего-рiях господарств становило: велико'!рогатог худоби - 130527, дрiбно'грогатог худоби - 44739, свиней - 520801, коней - 1781, пта-х1в - 15342087, у тому чиmi курей - 14816157, водоплавное - 525930. Упродовж 2014-2016рр. лабораторiями ветеринарног медицини у Днтропетровськш областi було проведено 18474 до^дження на iнфекцiйнi захворювання тварин. Всього було отримано 142 позитивн результати. За результатами бактерiологiчних до^джень у Днтропетровськш областi за 2014-2016 рр. було зареестровано диплококоз, злоятсний набряк, пастерельоз, колiбактерiоз, сальмонельоз, пулороз, стафтококоз, стрептококоз та бешиху свиней, псевдомоноз птищ, аеромоноз риб, парагнилець та протез бджт. На колiбактерiоз проведено 4472 до^дження i одержано 78 позитивних результатiв. Вiд свиней отримано 21 культуру, вiд птищ - 44 культури, вiд ембрютв - 6 культур, вiд дрiбно'г рогатог худоби - 3 культури, вiд хутрових звiрiв - 2 культури, вiд собак - 2 культури. Культури Escherichia coli, видтещ вiд свиней представлен серологiчними варiантами: O139, O26, О9, О78, О1, О8 та нетипованими, а вiд птиц - O115, 0119,078, О15, 086, К88 АБ та нетипованими. На сальмонельоз проведено 7578 до^джень i отримано 16 позитивнихрезультатiв. Серед хвороб бджт лабораторними до^дженнями було зареестровано парагнилець та протеоз бджт. Висновки: у Днiпропетровськiй областi бактерiологiчними до^дженнями видтено збудникв 13 тфекцшних хвороб тварин. Найчастше видтялися збудники кишкових тфекцш (колiбактерiозу та сальмонельозу).
Ключовi слова: Днтропетровська область бактерюзи, тварини, колiбактерiоз, сальмонельоз, бактерiологiчнi до^дження.
Introduction
Results and discussion
The modern path of development of the Ukraine causes appearance of complex requirements and tasks in the field of animal husbandry, directed on the provision of the population with high-quality agricultural production in accordance with international standards of quality and security. Such production of animal origin should not contain pathogenic microorganisms and can only be obtained from healthy animals (Khomenko et al., 1995).
The epizootic situation with regard to some infectious diseases in the Dnipropetrovsk region remains as complicated and strained (Tkachenko et al., 2007; Ukhovs'kij et al., 2014; Bila et al., 2014; Hlebenjuk, 2014; Hlebenjuk and Telizhenko, 2015; Borovyk, 2016). Timely and accurate diagnosis determines the effectiveness of the realized preventive measures of infectious diseases and establishes the peculiarities and changes of the epizootic situation in certain areas (Konopatkin et al., 1984; Yarchuk et al., 2002).
The laboratory examinations, including the bacteriological method, have the crucial importance for the correct assessment of prevention and antiepizootic measures (Vasylieva, 2016; Ushkalov, 2017).
Objective of this review: was to determine the etiological structure of infectious diseases in the Dnipropetrovsk region for 2014-2016, based on the results of bacteriological examinations.
Materials and methods
As the material for the examinations were the data of the veterinary reports of the State district veterinary medicine laboratories of the Dnipropetrovsk region and the results of our own examinations conducted on the basis of the Dnipropetrovsk Regional State Laboratory of the Veterinary Medicine (DRSLVM).
As of 2014 year, in the Dnipropetrovsk region, the live-stock capita in all categories of farms was: horned cattle - 130.527, small cattle - 44.739, pigs - 520.801, horses - 1.781, poultries - 15.342.087, including chickens - 14.816.157, waterfowl - 525.930.
During 2014-2016 by means of laboratories of veterinary medicine in the Dnipropetrovsk region have been conducted 18.474 examinations on the infectious diseases of the animals (Fig.). 142 positive results were received in total.
8000 n 7000 6000 5000 4000 3000 2000 1000 0
.A
2014
2015
2016
□ Samples examined
Fig. Number of the bacteriological examinations on bac-teriosis of the animals in the Dnipropetrovsk region for 2014-2016 (data, received from DRSLVM)
According to the results of the bacteriological examinations in the Dnipropetrovsk region, for the years 20142016 were registered diplococcosis, malignant edema, pasteurellosis, colibacteriosis, salmonellosis, pullorosis, staphylococcosis, streptococcosis and swine erysipelas, poultry pseudomonosis, aeromonosis of fish, parafoul brood (caused by Bacillus paraalvei) and proteose of bees.
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As can be seen from the table, 71 samples were examined on swine erysipelas, including 5 samples from wild fauna. The positive result was found in 5 animals from Kryvorizkyi and Petrykivskyi districts.
From the 745 examinations on pasteurellosis, the diagnosis was confirmed in 3 animals (2 rabbits from the city of Dnipro and 1 lamb from Pavlogradskyi district).
4.472 examinations were conducted on colibacteriosis and 78 positive results were obtained. 21 cultures were obtained from the pigs (Novomoskovs'kij, Sinel'nikivs'kij, Magdalinivs'kij, Dniprovskyi, Petrykivskyi and Pyatyhat-skyi districts), from poultries - 44 cultures (Novomoskovs'kij, Sinel'nikivs'kij, Krivoriz'kij, Tomakivs'kij, Dniprovskyi, Petrykivskyi and Pyatyhatskyi districts), from embryos - 6 cultures (Petrykivskyi district), from small cattle - 3 cultures (Magdalinivs'kij and Dniprovskyi districts), from fur animals - 2 cultures, from dogs - 2 cultures.
Cultures of Escherichia coli, isolated from pigs are represented by the serological variants: O139, O26, O9, O78, O1, O8 and atypical variants, and from poultries -O115, O119, O78, O15, O86, K88 AB and atypical variants.
For salmonella, 7,578 examinations were conducted and 16 positive results were obtained (Table). From pigs received 2 cultures (Krivoriz'kij and Petrikivs'kij districts), from horned cattle - 1 culture (Magdalinivs'kij district), from coypu furs - 2 cultures (Verch-nedniprovs'kij district), from poultries - 12 cultures (Magdalinivs'kij, Krivoriz'kij, Sinel'nikivs'kij and Pyaty-hatskyi districts).
Table
Frequency of isolation of bacterial pathogens of animals in Dnipropetrovsk region for 2014-2016
Name of Samples Cultures
disease examined isolated
colibacteriosis 4472 78
salmonellosis 7578 16
pullorosis 213 9
swine erysipelas 71 5
pasteurellosis 745 3
staphylococcosis 18 12
swine streptococcosis 27 4
poultry pseudomonosis 73 6
diplococcosis 1 1
malignant edema 1 1
aeromonosis of fish 360 5
parafoul brood 2 1
proteose of bees 1 1
rest 4912 0
Causative agent of pullorosis were isolated in 9 cases (Magdalinivs'kij and Pyatyhatskyi districts).
By means of bacteriological examinations were determined the etiological significance of 12 staphylococcal cultures in the occurrence of inflammatory processes (vaginitis, conjunctivitis, dermatitis, etc.). Diplococcosis was recorded in 1 animal (Magdalinivs'kij district).
Streptococcosis of pigs has been confirmed by 4 animals (Sinel'nikivs'kij district). For malignant edema of
swines, 1 examination was conducted and the positive result was obtained (Pyatyhatskyi district).
On pseudomonosis of the poultries, 73 examinatios were conducted and 6 positive results were obtained (Dniprovskyi and Pyatyhatskyi districts). In addition, the culture of Pseudomonas aeruginosa was isolated from the dog and lamb biomaterial.
From the 360 examinations on fish aeromonosis, the diagnosis was confirmed in 5 samples (Novomoskovs'kij and Dniprovskyi districts).
Among the diseases of bees, laboratory tests have been recorded parafoul brood and proteose of bees.
Conclusion
Causative agents of 13 infectious animal diseases have been identified in the Dnipropetrovsk region by means of bacteriological investigations. Most commonly isolated pathogens of intestinal infections (colibacteriosis and salmonellosis).
Prospects for further research are to conduct an analysis of bacteriological examinations on animal bacteriosis in the Ukraine and to evaluate the effectiveness of specific prevention of infectious diseases of animals in the Dnipropetrovsk region.
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