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Abstracts. PHYTOPHARM 2017
useful for consultation when treatment problems appear or during emergency situations [3]. The author has been involved in various research projects of basic and clinical aspects in mainly Chinese medicines among other herbal and pharmaceutical medicines hence examples will be given largely on those related to these disciplines as illustrations in this presentation.
References:
1. Chan K. 2016. World J Trad Chin Med. 2(4):6-28.
2. Williamson EM et al. 2014. Science: S21-S23.
3. Chan K et al. 2015. Br J of Clin Pharmacol. 80(4): 834-843.
EFFECT OF HELIOTROPIUM HIRSUTISSIMUM GRAUER METHANOL EXTRACT ON NF-KB PATHWAY IN RAT LIVER FOLLOWING PARTIAL HEPATECTOMY WITH MINI ARRAY ANALYSIS
© EmreCeyhan12, Ceyda Sibel Kilig3, Safa Gümü§ok3, Mediha Canbek1
1 Eski§ehirOsmangaziUniversity, Faculty of Science and Letters, Department of Biology, Division of Molecular Biology, Eski§ehir, Turkey;
2 Eski§ehirOsmangaziUniversity, Faculty of Medicine, Department of Biophysics, Eski§ehir, Turkey;
3 Ankara University Faculty of Pharmacy, Department of Pharmaceutical Botany, Ankara, Turkey
In this study, apoptotic effect of methanolic extract of the aerial parts of Heliotropium hirsutissimum GRAUER (Boraginaceae) on liver regeneration induced after 70% surgically formed partial hepatectomy (PH) was determined. This species is known to contain pyrrazolidin alkaloids and is hepatotoxic; however it is being used for different ailments in traditional medicine [1].
Methanolic extract of the aerial parts of H. hirsutissimum has has inhibitory effect on cell proliferation, as well [2]. Study was performed on Wistar albino male rats (5 groups, each group consisting of 3 rats, all processes performed following partial hepatectomy).While only laparotomy was performed on Sham control Group (Group I), a single dose of intraperitoneal (i.p.) methanol was administered to Group II. Group III, IV and V were administered 200 mg • kg-1, 400 mg • kg-1 and 800 mg • kg-1 extract, respectively via i.p. injection.
Rats were sacrificed 6 hours after the surgical procedure and their livers were removed. Stimulative
or preventive effects of different doses of extracts on 87 genes that are found in the NF-kB pathway were determined with mini array technique and it was demonstrated that intraperitoneally administered
H. hirsutissimum extract inhibited hepatocyte proliferation and induced apoptosis 6 hours after the performance of partial hepatectomy at doses of 400 mg • kg-1 and 800 mg • kg-1. Thus we can conclude that methanolic extract of this species has apoptotic effect on tumor cells in general. We are planning to continue our studies to determine the active substance(s) responsible for this effect and also to carry out our studies on different cancer series.
References:
I. Togola A, Diallo D, Dembele S, Barsett H and Paulsen BS, 2005. J Ethnobiol Ethnomedicine, 1(1):7.
2. Dash KG, Abdullah SM. 2013. Int J of Pharm Sci and Res. 4(4):1253-1258.
DETERMINATION OF THE ANTIOXIDANT EFFICACY OF EUPATORIUM CANDOLLEANUM EXTRACT BY NON-INVASIVE MEASUREMENTS
© Pérez Damonte Silvia2, Redko Flavia1, Repetto Marisa3, Teves Sergio4, Segall Adriana5, Clavin María L.1
1 Cátedra de Farmacognosia- IQUIMEFA (UBA_CONICET);
2 CLAIM, José Bonifacio 717, Buenos Aires, Argentina;
3 Cátedra de Química General e Inorgánica;
4 Cátedra de Microbiología;
5 Cátedra de Calidad de Medicamentos, Facultad de Farmacia y Bioquímica, Universidad de Buenos Aires
In previous work, some argentine plant species were selected to investigate different activities related to potential uses in cosmethic. Thus, the hidroalcoholic extract of the argentine medicinal species Eupatorium
candolleanum (ECH) presented high in vitro antioxidant activity [1]. These results make ECH a promisory active for the investigarion of its possible use as antioxidant. Thus, the aim of the present work was to evaluate the
Obzory po kliniceskoj farmacologii i lekarstvennoj terapii [Reviews of clinical pharmacology and drug therapy]
vol. 15/2017/suppLeMEnT 1
