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boiling waterbath, filtered, and then dried. Phytochemical investigation of these three extracts was carried out using standard methodology to identify and quantify the major chemical constituents. The total tannins content was estimated as tannin equivalents in dry A. eupatoria extract by titrimetrically. The total flavonoids content was estimated as rutin equivalents in dry extract of B. tripartita by spectrophotometric method. The total triterpene saponins content was estimated as p-escin equivalents in the dry extract of S. canadensis using spectrophotometric method. The antioxidant activity (AOA) was measured by coulometric titration. The following buffer solutions: acid potassium phthalate 0.2 mol L-1 (buffer solution, PH = 4.01);
potassium bromide 0.2 mol L-1; sulfuric acid 0.2 mol L-1. Injected sample volumes were: 0.1 ml, 0.2 ml, and 0.5 ml.
It was found that the dry extracts contained: total tannins 16.1 ± 0.8%; total flavonoids 19.0 ± 0.7%, and total saponins 7.2 ± 0.6%. Experimentally it was found that the use of the system without buffer solution gives similar results, therefore was not applied. The value of antioxidant activity for all extracts ranged from 9.5 to 10.5 mg/g and was comparable with AOA of standard sample of quercetin (10.1 mg/g).
Our results provide evidence for the potential usefulness of B. tripartite, S. canadensis and A. eupatoria dry extracts for new medicines of natural origin.
DETERMINATION OF EFFECT OF PRANTSCHIMGIN AND FELAMEDIN ON NF-KB PATHWAY IN RAT LIVER FOLLOWING PARTIAL HEPATECTOMY WITH MINI ARRAY ANALYSIS
© Ceyda Sibel Kiliq1. Emre Ceyhan2 3, Mediha Canbek2
1 Ankara University Faculty of Pharmacy, Department of Pharmaceutical Botany, Ankara, Turkey;
2 Eski§ehir Osmangazi University, Faculty of Science and Letters, Department of Biology, Division of Molecular Biology, Eski§ehir, Turkey;
3 Eski§ehir Osmangazi University, Faculty of Medicine, Department of Biophysics, Eski§ehir, Turkey
In this study, the effect of prantschimgin and felamedin (coumarins) isolated from Ferulago isaurica Pe§men (Apiaceae) and dissolved in EtOH was demonstrated in liver regeneration following 70% partial hepatectomy (PH).
Coumarins are the major active substances of the family Apiaceae and possess various biological activities [1]. Initiation of growth response in liver degeneration is due to the complex interactions between hepatocytes, non-parenchymal cells, extracellular matrix, metabolites and foodstuff. Regeneration response is via a couple of signal pathways and one of the most important signal pathways in stimulated liver following PH is the NF-KBsignal pathway [2, 3].
In order to show the triggering effect of these coumarin compounds on cell proliferation, we performed studies on Wistar albino male rats (5 groups, each containing 3 rats; all processes performed following partial hepatectomy). Group 1 (Sham control group) -
onlylaparotomy was performed; Group II a received single dose intraperitoneal^ (ip) administered methyl alcohol; Group III, IV and V were administered 100 mg • kg-1, 200 mg • kg-1 and 400 mg • kg-1 coumarin mixture, respectively via i.p. injection. Rats were sacrificed 6 hours after the surgical procedure, their livers were removed and examined with mini array analysis.
We have shown that 100 mg • kg-1 of prantschimgin and felamedin mixture induced liver regeneration following 70% PH, and therefore we can conclude that these coumarins are active in increasing the mitotic activity of the liver which is important in liver fibrosis.
References:
1. Gürbüz i, Erdurak CS, Coskun M, Yeçilada E, 2004.
Turkish J.Pharm.Sci. 1(1):47-53.
2. Fausto N, Campbell JS, 2003. Mech Dev, 120:117-130.
3. Zimmermann A, 2004. Nephrology Dialysis Transplantation, 19(4):iv6-iv10.
ANTI-INFLAMMATORY EFFECTS OF SANGUISORBAE RADIX ON CONTACT DERMATITIS INDUCED BY DINITROFLUOROBENZENE IN MICE
© Suzy Jo1, Junghyun Ryu1, Mi Heon Ryu2, Hyungwoo Kim1
1 School of Korean Medicine, Pusan National University, Pusan, South Korea;
2 School of Dentistry, Pusan National University, Pusan, South Korea
The dried root of Sanguisorba officinalis L. such as eczema, pruritus and urticarial in China, Japan (Sanguisorbae Radix) is used to treat skin diseases and Korea.
Obzory po kliniceskoj farmacologii i lekarstvennoj terapii [Reviews of clinical pharmacology and drug therapy]
vol. 15/2017/suppLeMEnT 1
We investigated the effects of Sanguisorbae Radix extract (SRE) on skin thickness, skin weights, histophathological changes and cytokine productions in dimitrofluorobenzene (DNFB)-induced CD mice.
Topical application of SRE effectively inhibited enlargement of skin thicknesses (P < 0.05, 27.8% of inhibition compared to CTL) and weights (P < 0.05, 22.4% of inhibition compared to CTL). SRE treatment also inhibited epidermal hyperplasia, hyperkeratosis and immune cell infiltration induced by DNFB in inflamed tissues in CD mice. Moreover, treatment with SRE suppressed the increase in the levels of TNF-a (P < 0.01, 38.9% of inhibition compared to CTL)
and IFN-y (P < 0.05, 31.9% of inhibition compared to CTL) respectively. These data suggest that SRE acts as an anti-inflammatory agent that decreases the production of TNF-a and IFN-y, resulting in ameliorating histopathological abnormalities in inflamed skin tissues.
References:
1. Abrahaamson MJ, 2004. Arch. Intern. Med, 164:486491.
2. Stand E, Baumgartl HJ, Füchtenbusch M, Stem-plinger J, 1999. Diabetes. Obes. Meta., 1:215-220.
3. Hanefeld M. 1998. J. Diabetes Complications, 12:228237.
PHENYLPROPANOIDS FROM IXERIS DENTATA AND THEIR NEURAMINIDASE INHIBITORY ACTIVITY
© Seon Ju Park, Jun Hyung Park, Seung Hyun Kim
College of Pharmacy, Yonsei Institute of Pharmaceutical Science, Yonsei University, Korea
Ixeris dentata (Asteraceae) is well-known for edible wild vegetable in Asia, which is distributed throughout East Asia [1]. Using various chromatographic methods, seven phenylpropanoid glycosides (1-7), two flavonoid glycoside (8, 9) and one alkene glycosides (10) were isolated. Their structures were determined as chlorogenic acid (1), 3,5-dicaffeoylquinic acid (2), 4,5-dicaffeoylquinic acid (3), citrusin C (4), 4-allyl-2,6-dimethoxyphenol glucoside (5), diydrosyringin (6), 3-(4-hydroxy-3,5-dimethoxyphenyl) propyl p-D-glucopyranoside (7), apigenin 7-O-p-D-glucoside (8), luteolin 7-O-p-D-glucoside (9) , and (Z)-3-hexenyl-p-D-glucopyranoside (10), respectively, by spectroscopic methods including NMR and MS. All
the isolated compounds were tested for their ability to inhibit neuraminidase. Two quinic acid derivatives (2, 3) significantly inhibited neuraminidase activity, which was comparable to the positive control, mangiferin [2]. In addition, structure-based virtual screening against neuraminidase showed.
References:
1. Barbero F, Maffei M. 2016. Plant Specialized Metabolism: Genomics, Biochemistry, and Biological Functions. United Kingdom: Taylor & Francis Incorporated.
2. Karar MGE, Matei MF, Jaiswal R, Illenberger S, Kuhnert N. 2016. Food Funct. 7:2052-2059.
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