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useful for consultation when treatment problems appear or during emergency situations [3]. The author has been involved in various research projects of basic and clinical aspects in mainly Chinese medicines among other herbal and pharmaceutical medicines hence examples will be given largely on those related to these disciplines as illustrations in this presentation.
References:
1. Chan K. 2016. World J Trad Chin Med. 2(4):6-28.
2. Williamson EM et al. 2014. Science: S21-S23.
3. Chan K et al. 2015. Br J of Clin Pharmacol. 80(4): 834-843.
EFFECT OF HELIOTROPIUM HIRSUTISSIMUM GRAUER METHANOL EXTRACT ON NF-KB PATHWAY IN RAT LIVER FOLLOWING PARTIAL HEPATECTOMY WITH MINI ARRAY ANALYSIS
© EmreCeyhan12, Ceyda Sibel Kilig3, Safa Gümü§ok3, Mediha Canbek1
1 Eski§ehirOsmangaziUniversity, Faculty of Science and Letters, Department of Biology, Division of Molecular Biology, Eski§ehir, Turkey;
2 Eski§ehirOsmangaziUniversity, Faculty of Medicine, Department of Biophysics, Eski§ehir, Turkey;
3 Ankara University Faculty of Pharmacy, Department of Pharmaceutical Botany, Ankara, Turkey
In this study, apoptotic effect of methanolic extract of the aerial parts of Heliotropium hirsutissimum GRAUER (Boraginaceae) on liver regeneration induced after 70% surgically formed partial hepatectomy (PH) was determined. This species is known to contain pyrrazolidin alkaloids and is hepatotoxic; however it is being used for different ailments in traditional medicine [1].
Methanolic extract of the aerial parts of H. hirsutissimum has has inhibitory effect on cell proliferation, as well [2]. Study was performed on Wistar albino male rats (5 groups, each group consisting of 3 rats, all processes performed following partial hepatectomy).While only laparotomy was performed on Sham control Group (Group I), a single dose of intraperitoneal (i.p.) methanol was administered to Group II. Group III, IV and V were administered 200 mg • kg-1, 400 mg • kg-1 and 800 mg • kg-1 extract, respectively via i.p. injection.
Rats were sacrificed 6 hours after the surgical procedure and their livers were removed. Stimulative
or preventive effects of different doses of extracts on 87 genes that are found in the NF-kB pathway were determined with mini array technique and it was demonstrated that intraperitoneally administered
H. hirsutissimum extract inhibited hepatocyte proliferation and induced apoptosis 6 hours after the performance of partial hepatectomy at doses of 400 mg • kg-1 and 800 mg • kg-1. Thus we can conclude that methanolic extract of this species has apoptotic effect on tumor cells in general. We are planning to continue our studies to determine the active substance(s) responsible for this effect and also to carry out our studies on different cancer series.
References:
I. Togola A, Diallo D, Dembele S, Barsett H and Paulsen BS, 2005. J Ethnobiol Ethnomedicine, 1(1):7.
2. Dash KG, Abdullah SM. 2013. Int J of Pharm Sci and Res. 4(4):1253-1258.
DETERMINATION OF THE ANTIOXIDANT EFFICACY OF EUPATORIUM CANDOLLEANUM EXTRACT BY NON-INVASIVE MEASUREMENTS
© Pérez Damonte Silvia2, Redko Flavia1, Repetto Marisa3, Teves Sergio4, Segall Adriana5, Clavin María L.1
1 Cátedra de Farmacognosia- IQUIMEFA (UBA_CONICET);
2 CLAIM, José Bonifacio 717, Buenos Aires, Argentina;
3 Cátedra de Química General e Inorgánica;
4 Cátedra de Microbiología;
5 Cátedra de Calidad de Medicamentos, Facultad de Farmacia y Bioquímica, Universidad de Buenos Aires
In previous work, some argentine plant species were selected to investigate different activities related to potential uses in cosmethic. Thus, the hidroalcoholic extract of the argentine medicinal species Eupatorium
candolleanum (ECH) presented high in vitro antioxidant activity [1]. These results make ECH a promisory active for the investigarion of its possible use as antioxidant. Thus, the aim of the present work was to evaluate the
Obzory po kliniceskoj farmacologii i lekarstvennoj terapii [Reviews of clinical pharmacology and drug therapy]
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integrity of skin barrier in healthy volunteers treated with ECH, by meassuring its response on the transdermal loss of water (TEWL) and the effect on the erythema induced by capsaicin (EIC). Also to carry on preliminar studies of chemical composition and chromatographic profiles of the active extract.
ECH was prepared with the ground dried aerial parts of E. candolleanum in ethanol 80%, under reflux for 2 hours. The extract obtained was concentrated and redissolved in propilenglicol-ethanol 99:1, 1 mg/ml. TEWL and EIC assays were performed on healthy women volunteers, according to International rules. ECH was applied on the womens' internal forearm, twice a day during 21 days. TEWL and EIC were evaluated the first day and after 21 days. TEWL was meassured using a Tewameter® (Courage &Khazaka, Germany). Messurement of EIC was done with a colorimeter
Chromameter® CR400 (Minolta, Japan). The t Student test was applied to determine significances in the different meassurements.
The topical administration of ECH on the volunteers significantly decreased TEWL values in a 17.59%. ECH diminished erythema in 35,76 % respect to non treated individuals.
TEWL's decrease by ECH demonstrates the efficacy of this natural ingredient on the barrier function of skin. Capsaicin produces irritation and inflammation, which generates free radicals responsible of skin redness [2]. So, the decrease of the erythema produced by ECH reveals its antioxidant capacity.
References:
1. Clavin et al, 2014. Cosmética, 89(1):17-25.
2. Kohen R, 1999. Biomed Pharmacother, 53(4):181-192.
IN-VITRO ANTIPROLIFERATIVE ACTIVITY OF TRITERPENES ISOLATED FROM TWO MEDICINAL NATIVE PLANTS OF MEXICO
© Díaz Sánchez Lidia1, Dehesa Zentella Alejandro2, García Veronica1, Ramírez-Apan Maria Teresa1, Martínez-Vázquez Mariano1
11nstituto de Química, Universidad Nacional Autónoma de México, Cd Universitaria, circuito exterior, Insurgentes Sur, CDMX; 2 Instituto Nacional de Ciencias Médicas y Nutrición Salvador Zubirán, Vasco de Quiroga 15, Tlalpan, Ciudad de México, CDMX
The World Health Organization (WHO) estimates that in 2015 approximately 9 million people died from cancer [1]. Although several anticancer drugs have been tested however due to their toxicity and limited therapeutic activity it is necessary to look for new anticancer drugs. An approach that has allowed the obtaining of several anticancer drugs nowadays used in the clinic is the one related to natural products. For example, vinca alkaloids isolated from Cantharatus roseus, taxol isolated from Taxus brevifolia and others, indicate the relevance of using naturally occurring secondary metabolites (SM) as anticancer drugs. Mexico has an extensive repertoire of plant species in use within ethnopharmacology. In this sense, our group was particularly interested in two species: Amphipterygium adstringens (Anacardiaceae), known as "cuachalalate", and Hippocratea excelsa (Hippocrateaceae), known as "cancerine." Both are native plants of Mexico, and are of interest because they are widely used to treat different conditions; In particular, decoction of the cortex is commonly used in the treatment of gastric ulcers, various inflammatory conditions and different types of cancer [2]. Based on the above, we set out to isolate the SM present in the bark of both plants to evaluate their antiproliferative activity. The isolated SM of the "cuachalalate" were the triterpenic masticadienonic (AMD) and 3a-hidroximasticadienonic (3a-AMD) acids, whereas of the cancerine the canophyllol, HeHx-H and HeHx-DA were isolated. The elucidation of the compounds was performed by
proton nuclear (M) nuclear magnetic resonance (NMR). Subsequently, in-vitro antiproliferative evaluation of the 5 compounds was performed (as positive control and reference drug, cisplatin was used) according to the protocol of Vanish Vichai and KanyawinKirtikara [3] in two different human cancer lines: PC3 (prostate cancer) and HCT15 (colon cancer). Through the results obtained in this test IC50 were determined for each compound (Table 1). The results of our studies indicate that SM tested are partly responsible for the anti-proliferative activities of the plants studied.
IC50 (^M)
Cispla-tino AMD 3a-AMD HeHx-H Cano-phylol HeHx-DB
PC3 8.525 19.7 12.3 31.25 12.5 70
HCT15 11 10.3 7.82 23.75 35 80
References:
1. World Health Organization, 2017 Cáncer. http://www. who.int/mediacentre/factsheets/fs297/es/.
2. Oviedo-Chávez I, Ramírez-Apan T, Soto-Hernández M and Martínez-Vázquez M, 2004. Phytomedi-cine, 11:436-445.
3. Vichai V, Kirtikara K, 2006. Nature protocols, 1(3): 1112-1116.
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