Научная статья на тему 'GC-MS Analysis of Phytochemical Constituents from Ethyl Acetate and Methanol Extract of Artocarpus altilis (Parkinson) Fosberg from Endau Rompin, Johor, Malaysia'

GC-MS Analysis of Phytochemical Constituents from Ethyl Acetate and Methanol Extract of Artocarpus altilis (Parkinson) Fosberg from Endau Rompin, Johor, Malaysia Текст научной статьи по специальности «Фундаментальная медицина»

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Artocarpus altilis / GC-MS / medicinal plants / pharmacology / phytochemical compounds

Аннотация научной статьи по фундаментальной медицине, автор научной работы — Muhammad Murtala Mainasara, Mohd Fadzelly Abu Bakar, Aisha Ibrahim Barau

In the present days, medicinal plants receive great attention to the researchers in the field of pharmacology, due to the fact that most of the drug industries rely on natural products more especially medicinal plants for the production of new drugs. Some traditional medicines and their derived products were often made from crude plant extracts, which include a mixture of complex different phytochemical constituents. The chemical features of these constituents vary substantially among different species. Gas chromatography and Mass spectroscopy (GC-MS) method used for the analysis of the obtained extracts can be a remarkable tool for screening the quantity of some active principles in herbs used in pharmaceutical industries. The identification of secondary metabolites is based on the peak area, retention time molecular weight, molecular formula, MS Fragmentions and Pharmacological actions. The aim of this study was to carry out for identification of bioactive compounds from the leaf ethyl acetate and methanolic extract of Artocarpus altilis by (GC-MS). Fifty and fifteen bioactive phytochemical compounds were identified in the methanolic and ethyl acetate extracts respectively. GC/MS analysis revealed the existence of Acetic acid, n-Hexadecanoic acid, 1,2,3-Propanetriol, 1-acetate (Acetin), Hexadecanoate <methyl->, 7-Tetradecenal, in the methanol extract while the ethyl acetate extract revealed the presence of 3, 7-dimethyl-2, 6-octadienal (Geranial-pseudo phytol), 3,7,11,15Tetramethyl-2-hexadecen-1-ol (Phytol), Piperonal and Heliotropin. Further studies are needed to isolate active principle of the extract as well as to elucidate their exact mechanism of action in various disorders.

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Текст научной работы на тему «GC-MS Analysis of Phytochemical Constituents from Ethyl Acetate and Methanol Extract of Artocarpus altilis (Parkinson) Fosberg from Endau Rompin, Johor, Malaysia»

GC-MS Analysis of Phytochemical Constituents from Ethyl Acetate and Methanol Extract of Artocarpus altilis (Parkinson) Fosberg from Endau Rompin, Johor, Malaysia

Muhammad Murtala Mainasara 1 2, Mohd Fadzelly Abu Bakar Aisha Ibrahim Barau 3

1 Universiti Tun Hussein Onn Malaysia

101 Parit Raja, Batu Pahat, Johor, 86400, Malaysia

2 Usmanu Danfodiyo University P. M. B. 2346, Sokoto, Nigeria

3 Kaduna State University

P. M. B 2339, Kaduna State, Nigeria

DOI: 10.22178/pos.46-2

LCC Subject Category: QH301-705.5

Received 20.04.2019 Accepted 27.05.2019 Published online 31.05.2019

Corresponding Author: Muhammad Murtala mmgusau96@gmail.com

© 2019 The Authors. This article is licensed under a Creative Commons Attribution 4.0 License IOL.

Abstract. Wn the present days, medicinal plants receive great attention to the researchers in the field of pharmacology, due to the fact that most of the drug industries rely on natural products more especially medicinal plants for the production of new drugs. Some traditional medicines and their derived products were often made from crude plant extracts, which include a mixture of complex different phytochemical constituents. The chemical features of these constituents vary substantially among different species. Gas chromatography and Mass spectroscopy (GC-MS) method used for the analysis of the obtained extracts can be a remarkable tool for screening the quantity of some active principles in herbs used in pharmaceutical industries. The identification of secondary metabolites is based on the peak area, retention time molecular weight, molecular formula, MS Fragment- ions and Pharmacological actions. The aim of this study was to carry out for identification of bioactive compounds from the leaf ethyl acetate and methanolic extract of Artocarpus altilis by (GC-MS). Fifty and fifteen bioactive phytochemical compounds were identified in the methanolic and ethyl acetate extracts respectively. GC/MS analysis revealed the existence of Acetic acid, n-Hexadecanoic acid, 1,2,3-Propanetriol, 1-acetate (Acetin), Hexadecanoate <methyl->, 7-Tetradecenal, in the methanol extract while the ethyl acetate extract revealed the presence of 3, 7-dimethyl-2, 6-octadienal (Geranial-pseudo phytol), 3,7,11,15Tetramethyl-2-hexadecen-1-ol (Phytol), Piperonal and Heliotropin. Further studies are needed to isolate active principle of the extract as well as to elucidate their exact mechanism of action in various disorders.

Keywords: Artocarpus altilis; GC-MS; medicinal plants; pharmacology; phytochemical compounds.

INTRODUCTION

The genus Artocarpus belongs to the family Moraceae which comprises about 60 genera and over 1000 species. Many of these species are used as a source of food and in traditional medicinal practices [14]. Artocarpus species are known for its large edible fruit with high nutritive values. The important species belonging to this genus are A. heterophyUus, A. altilis, A. hirsu-tus, A. lakoocha and A. camansi. Other than fruits and seeds, extracts of aerial and underground

parts have been used traditionally in the treatment of diabetes, diarrhoea, dermatitis, malarial fever, asthma, tapeworm infection, anaemia and many other diseases [14]. Also the plant latex has significant contribution in medicine as it contains biologically active compounds such as alkaloids, flavonoids, terpenoids, glycosides, phenolics, tannins and saponins etc. which are not only beneficial for plant defence but also for the development of things such as disinfectants, anticoagulant [8]. Islanders use the latex of A. altilis to

treat skin diseases, stomach ache, diarrhoea and dysentery [30]. Crushed leaves are used to treat skin, ear and eye infections. Other medicinal uses include the roots as stringent, purgative and poultice for skin ailments, and the bark for treating headache.

The fruit is an excellent source of fibre, calcium, copper, iron, magnesium, potassium, thiamine, niacin, carbohydrates, and vitamin [1]. These plants are known to contain many potential bio-active phytochemicals which possess many validated pharmacological properties [13]. Artocar-pus species are mainly distributed in tropical and subtropical regions of Asia A. altilis (breadfruit) is native to New Guinea, Indonesia and Philippines. Currently, they are cultivated in central and South America, Africa, India, Southeast Asia, Maldives, Indonesia, Srilanka and northern Australia. A. altilis (Parkinson) Fosberg (breadfruit). Synonyms of A. altilis are A. communis and A. incisus). The generic name of the species comes from the Greek words 'artos' (bread), and 'karpos' (fruit) and the fruits eaten are commonly called breadfruit [34]. In general, breadfruit trees are very large, an evergreen which can reach heights of 15 to 20 meters. The tree comprises smooth, light-coloured bark, and the trunk is large in 1.2 m in diameter, occasionally growing to a height of 4 m before branching. The wood is gold in colour, but when contact with air, turns to a darker colour. Latex can be seen in all parts of the tree which are milky [34].

Breadfruits are available with seeds and also without seeds, the seeded types of breadfruit are available in south-western Pacific, whereas seedless types of breadfruit are common in Micronesia and Eastern islands of Polynesia. All the breadfruit varieties elsewhere, especially in the topic region, are of a seedless type. Seeds are brown in colour, shiny, round or ovoid and irregularly compressed. Moreover, the seeds have little or no endosperm, no period of dormancy and they can germinate immediately. Since they can germinate immediately, they are not able to be dried or stored. Trees that grow with the help of seeds can produce their fruits in a timeline of 6-10 years or sooner. On the contrary, asexually propagated trees can start to produce their fruits in about three to six years of time.

Fruits of breadfruit are nutritious and are consumed as a starchy staple when mature. They are rich in carbohydrates and contain vitamins and minerals. A fruit quality evaluation of 20 bread-

fruit cultivars sampled from the National Tropical Botanical Garden in Hawaii showed significant differences in aroma, texture, colour, flavour, sweetness, starchiness, moistness, stringi-ness and firmness [30]. The most significant differences were in colour and texture. Nutrient analyses showed that the pulp of mature fruits of A. altilis (100 g) contained 69% of water, 1.0 g of protein, 29 g of carbohydrate, 5.2 g of dietary fibre, 22 mg of sodium, 24 mg of magnesium, 32 mg of phosphorous, 350 mg of potassium and 20 mg of calcium on the average. The content of vitamin C, p-carotene and lutein was 3.8 mg, 13 ^g and 72 ^g respectively. Breadfruit is a good source of vitamin C, thiamine, riboflavin and nia-cin. Nutritionally, breadfruit is comparable or superior to other staple food commonly consumed in Oceania, e.g., taro, plantain, cassava, sweet potato and rice.

Artocarpus genus can produce a large number of secondary metabolites usually abundant in phenylpropanoids such as flavonoids and fla-vones. They also produce phenolic compounds including flavonoids, stilbenoids and arylbenzo-furons. Over 130 compounds are identified in various organs of A. altilis, more than 70 of which derived from the phenylpropanoid pathway [34]. Many of the isolated compounds exhibit biological activity such as inhibit platelet aggregation, anti-bacterial activity, anti-fungal properties, inhibition of leukaemia cells and as an anti-tumour agent [6]. Some of the bioactive compounds that were isolated and found to be responsible for the anticancer activities from A. altilis that exhibited good activity are pyranocycloartobiloxanthone A (PA), dihydro-artoindonesianin C, and pyrano-cycloartobiloxanthone B isolated from A. obtusus [27]. Nutritional compositions of the seeds have water, protein, carbohydrate, fat, calcium, phosphorus, iron, niacin, thiamine and vitamin C [9]. A. altilis contains some chemical constituents such as morin, moracin, dihydromorin, cy-nomacurin, Early chemical analysis of fruits of A. altilis led to the isolation of triterpenes of cycloartenol, cycloart-23-ene- 3p,25-diol, cycloart-25-ene-3p,24-diol and a-amyrin volatile chemicals of fresh and cooked fruits of A. altilis have been studied. In fresh breadfruit, 40 volatile compounds were identified with cis-3hexanol (36 %) being the major constituent. Out of 43 volatile compounds identified in breadfruit boiled for 10 min, the main component was ethyl acetate (38 %). From the methanol and ethyl acetate fruit extracts of A. altilis, arylbenzofuran of

moracin M; stilbenes of oxyresveratrol and ar-toindonesianin F; flavonoids of norartocar-panone, Artocapin, norartocarpetin and isoarto-carpesin; triterpenes of 3pacetoxyolean-12-en-11-one and cycloartenol acetate; and sterols of sitosterol p-Dglucopyranoside and sitosterol

have been isolated [22]. A. altilis have been reported to possess antioxidant and antimicrobial activities, a comparative study has been conducted on the antioxidant properties of the pulp, peel and whole fruit of A. altilis extracted with hexane, dichloromethane and methanol [22].

Figure 1 - Leaves, Stem and Fruits of A. Altilis

MATERIALS AND METHODS

The study was conducted in the rainforest of Kampung Peta located border to the north-east of Endau, Johor Darul Takzim and south to Rompin, Pahang. The forest (2°25'12.94" N, 103°15'40.94" E) is among the few lasting virgin lowland rainforest areas in the southern part of Peninsular Malaysia. The state government of Johor in 1993 gazette 870 km2 or 48,905 hectares of the Taman Negara Johor Endau Rompin (TNJER) forest as a national park [21].

The fresh sample (leaves) were collected under a permit granted by Perbadanan Taman Negara Johor (PTNJ) following the WHO guidelines on Good Agricultural and Collection Practices for Medicinal Plants. The samples as wild types from various locations earlier mentioned in May 2017. Roughly 1 to 4 kg of the fresh samples were collected and placed into a labelled plastic bags.

Plant materials were carefully clean and rinse by using distilled water to remove contaminant or soil debris. The samples were dried in the shade at room temperature and ground in a mortar or dried in the oven for two days at 40 °C. The dried sample will grinded to a fine powder using a dry grinder or pestle and mortar, the ground sample was kept in ziplock bag and to be stored in a freezer (-20 °C) for further analysis [20, 32].

The extraction of plant samples using organic solvents was carried out by successive maceration extraction as previously described by [7] with few modifications. Organic solvents with different polarities such as nhexane (non-polar), ethyl acetate (intermediate polar) and methanol (highly polar) were used. All the organic solvents used were obtained from Merck, Germany. One hundred grams of powdered plant materials were sequentially macerated with the specified volume of ethyl acetate and methanol in the order of increasing polarity of the solvents in 1:5 ratios in an enclosed flask with occasional shaking. The extraction was repeated three times until complete extraction. The mixture was kept at room temperature for 24 h. At each stage of extraction, the sample debris produced after filtration was left in a sterile fume hood to dry before being used in the subsequent extraction stage. The solvent from each sample was then filtered through a vacuum filter and then evaporated to a minimum volume under reduced pressure in a rotary evaporator set at 40 °C in a water bath. The resultant dried crude extracts from each plant were packed in glass bottles with accurate labels, and the yield of extracts was calculated using the formula below. Then the extracts were stored at 4 °C in a refrigerator until use.

The extraction yield was determined to be used as an indicator of the effects of the extraction

condition and expressed as percentage using the formula below (1).

Percentage yield =

Mass of recovered crude extract , ^ (1)

=---x100

Mass of starting plant material

The gas chromatography-mass spectroscopy (GC-MS) analysis was conducted on methanol extract of A. altilis (AAM) and ethyl acetate extract (AAE). The peaks (Figure 2) in the chroma-

togram were integrated and compared with the database of spectra of known compounds stored in the GC-MS libraries of National Institute Standard and Technology, WILEY229.LIB, Pfleger-Maurer-Weber-Drugs-and-. Pesticides-

Library for toxicology (PMW_tox2) and Flavour, Fragrance, Natural and Synthetic Compounds (FFNSC1.3.lib). The detailed tabulation of GC-MS analysis of the selected active extracts was given below in Table 1 and 2.

Table 1 - Phytochemical compounds identified in methanol extract of A. altilis

Peak No RT (min) Name of identified compounds Area (%) S/I

1 2.617 Acetic acid 34.68 98

29 50.958 n-Hexadecanoic acid 14.89 92

9 15.116 1,2,3-Propanetriol, 1-acetate (Acetin) 7.28 95

28 49.678 Hexadecanoate <methyl-> 4.01 94

11 22.122 Glycerol .alpha.-monoacetate 2.80 95

36 56.595 7-Tetradecenal, (Z)- 2.92 86

33 55.808 Phytol 2.46 93

37 57.302 Octadecanoic acid 2.26 91

50 86.925 Cholesterol 2.10 73

30 51.969 Palmitic acid 1.35 78

32 55.412 Linolenate <methyl-> 1.32 86

27 49.081 8- Oxabicyclo [5.1.0]oct- 5-en-2-ol, 1,4,4-trimethyl 1.28 78

18 38.767 Megastigmatrienone 1.28 87

8 12.341 1,2,3-Propanetriol 1.23 84

19 40.163 4,4,5,8-Tetramethylchroman-2-ol 1.22 72

12 22.328 Guaiacol <4-vinyl-> 1.20 88

35 56.404 cis-9,cis-12-Octadecadienoic acid 1.18 89

2 2.773 2-Propanone, 1-hydroxy- Acetol 1.08 97

25 46.659 Neophytadiene 1.06 93

23 44.975 Methylhydroquinone, 1.04 84

10 17.313 2,3-dihydro-3,5-dihydroxy-6-methyl-4h-pyran-4-one 0.99 90

15 34.157 (Phenol) 2,4-bis(1,1-dimethylethyl)- (CAS) 2,4-Di-tert-butylphenol 0.94 83

43 67.225 2-hydroxy-1 - (hydroxymethyl) ethyl ester 0.84 85

31 55.215 9,12-Octadecadienoic acid (Z,Z)-, methyl ester (Linoleic acid) 0.82 91

34 56.226 Methyl stearate 0.76 91

47 82.437 Vitamin E 0.61 88

4 3.735 :2-Propyn-1-ol 0.57 92

6 7.761 3(5)-d1-1,2,4-triazole 0.55 85

20 43.046 Coniferyl alcohol 0.53 81

41 62.105 Methyl 12-hydroxyoctadecanoate 0.51 79

17 38.294 Megastigmatrienone 0.51 80

21 44.115 2(4H)-Benzofuranone, 5,6,7,7a-tetrahydro-6-hydroxy-4,4,7a-trimethyl-, (6S-cis)- (CAS) Loliolide 0.48 87

40 60.943 Octanoic acid 0.36 89

13 25.371 Triacetin 0.35 73

49 86.605 16-Hentriacontanone 0.35 76

39 60.231 14-.beta.-h-pregna 0.34 83

7 7.955 2-Hydroxy-2-cyclopenten-1-one 0.32 86

24 45.945 Cyclopentanecarboxylic acid 0.32 77

Peak No RT (min) Name of identified compounds Area (%) s/i

22 44.551 4-(3-Hydroxy-2,2,6-trimethyl-7-oxa-bicyclo[4.1.0]hept-1-yl)-but-3-en-2-one 0.30 73

16 36.894 4-[(1E)-1,3-Butadienyl]-3,5,5-trimethyl-2-cyclohexen-1-one 0.28 80

5 3.983 Methyl acetate 0.26 84

26 48.163 2,6,10 -trimethyl, 14- ethylene- 14-pentadecne 0.25 88

3 3.430 Glycerin 0.21 87

14 27.066 2,4-bis(1,1-dimethylethyl)- (CAS) 2,4-Di-tert-butylphenol 0.21 93

42 63.008 2H-Pyran-2-one, tetrahydro-6-tridecyl- 0.21 77

Total compounds 45

Total Identified 98.49

Table 2 - Phytochemical compounds identified in the Ethyl acetate crude extract of A. altilis using GC-MS analysis

Peak No RT (min) Name of identified compounds Area (%) s/i

11 59.717 3, 7-dimethyl-2, 6-octadienal (Geranial-pseudo phytol) 59.95 99

5 49.566 3,7,11,15 -Tetramethyl-2-hexadecen- 1-ol (Phytol) 8.80 93

8 53.646 Piperonal, (Heliotropin) 7.22 88

12 65.428 3,7,11,15-Tetramethyl-1,6,10,14-hexadecatetraen-3-ol; (6E,10E)-geranyllinalool 4.41 96

7 50.991 Phytol acetate 3.13 96

3 25.987 Stearic acid; n-Octadecanoic acid 3.06 92

14 65.525 Neral (Pseudo-phyto<6Z,10Z) 2.28 89

6 50.408 Citronellyl pentanoate 2.02 97

13 65.462 Farnesyl acetate (Farnesol) 1.92 98

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9 54.665 Palmitic acid (Hhexadecanoic acid) 1.65 94

2 18.662 1,2,4,5-Tetramethylbenze (Durol) 1.53 89

10 59.162 7,11-Dimethyldodeca-2,6,10-trien-1-ol 1.35 97

4 26.238 1,2,3-triacetoxypropane (Triacetin) 1.08 83

1 4.197 4-hydroxy- 4- methyl- 2-pentanone 0.92 95

15 67.139 Presilphiperfolan-8-ol 0.68 91

Total compounds 15

Total Identified 100

RESULTS AND DISCUSSION

The quantity of recovered crude extracts varied according to the solvent used. It has been shown that methanol yielded relatively higher amounts of crude extracts. A. altilis (leaves) extracted with methanol yielded the highest crude extract 14.20%, while ethyl acetate had 5.83 %, from approximately 2-3 kg of shade dried powdered leaves. Most of the constituents were polar in nature.

The result of AAM revealed 50 peaks (Figure 2 (A), with 45 compounds identified (Table 1) representing 98.49% of the entire extract. The major among them were Acetic acid (34.68%), n-Hexadecanoic acid (14.89 %), 1,2,3-Propanetriol, 1-acetate (Acetin) (7.28 %), Hexadecanoate <methyl-> (4.01 %), 7-Tetradecenal, (Z)- (2.92%) Glycerol .alpha.-monoacetate (2.80 %), Phytol (2.46%), Octadecanoic acid (2.26 %), Cholesterol

(2.10%), Palmitic acid (1.35%), , Linolenate <methyl-> (1.32%), Megastigmatrienone and 8-Oxabicyclo[5.1.0]oct-5-en-2-ol, 1,4,4-trimethyl (1.28%) each, 1,2,3-Propanetriol (1.23%) and 4,4,5,8-Tetramethylchroman-2-ol (1.22%).

The result of AAE revealed 15 peaks (Figure 2(B), with 15 compounds identified (Table 2) representing 100% of the entire extract. The major among them were 3, 7-dimethyl-2, 6-octadienal (Geranial-pseudo phytol) (59.95%), 3,7,11,15-Tetramethyl-2-hexadecen-1-ol (Phytol) (8.80%), Piperonal, (Heliotropin) (7.22%), 3,7,11,15-Tetramethyl-1,6,10,14-hexadecatetraen-3-ol; (6E,10E)-geranyllinalool (4.417 Phytol acetate (3.13%), Stearic acid; n-Octadecanoic acid (3.06%), Neral (Pseudo-phyto<6Z,10Z) (2.28%), Citronellyl pentanoate (2.02%), Farnesyl acetate (Farnesol) (1.92%), Palmitic acid (H-hexadecanoic acid) (1.65%) 1,2,4,5-Tetramethylbenze (Durol) (1.53%), 7,11-

Dimethyldodeca-2,6,10-trien-1-ol (1.35%) and 1,2,3-triacetoxypropane (Triacetin) (1.08%).

A

B

Figure 2 - GC chromatograms of (A) AAM (B) AAE

Tropical rainforests contain a lot of interesting pharmacologically active constituents, and many more are still waiting to be discovered as they still offer undoubtedly valuable and amazing chemical entities [15]. Natural products are the chemical compounds found in nature that usually has a pharmacological or biological activity for use in pharmaceutical drug discovery and drug design. The chemical constituents in the plants or crude extracts are known to be biologically active ingredients. Some chemical constituents are considered as secondary metabolites components. They are directly responsible for different activity such as antioxidant, antimicrobial, antifungal and anticancer [16]. Many anticancer drugs have been showing a clinical success were elaborated from naturally occurring molecules or developed from their synthetic analogues. Great interest is currently being paid to natural products because of their interesting anticancer activity [31]. Drugs of natural origin have been classified as original natural products; products derived semi-synthetically from natural products or synthetic products based on natural product models [10].

Collectively, plants produce a remarkably diverse array of over 100,000 low molecular-mass natural products, al o known as secondary metabolites. Secondary metabolites are distinct from the components of intermediary (primary) metabolism in that they are generally nonessential for the basic metabolic processes of the plant. Many secondary metabolites have been isolated and characterised from a variety of natural sources, such as bacteria, fungi, and plants. They are of

high interest and importance because they often exhibit a broad spectrum of biological activities. Phytochemicals are nonnutritive chemicals and responsible for the medicinal properties of plants [28, 38]. Different crude extracts were obtained from the leaves of A. altilis through successive maceration with solvents of increasing polarity, viz., ethyl acetate and methanol. GC-MS analysis of ethyl acetate and methanol extracts revealed the presence of various bioactive compounds.

Phytol is present both in methanol (2.46 %) and ethyl acetate extracts (8.80 %) but in different quantities. This compound is a diterpene, a member of the group of branched chain unsatu-rated fatty alcohols. It is the product of chlorophyll metabolism in plants that is abundantly present [11]. The literature revealed phytol have vast biological activities including anti-inflammatory, antimicrobial, cytotoxic, neuropro-tective, antidiabetic and antioxidant [19]. Some previous studies proved that phytol exhibited cancer preventive and antioxidant [35, 36, 37] and breast cancer specifically [12, 18, 33].

Hexadecanoic acid was also identified in both methanol (4.01%) and ethyl acetate (1.65%) extracts. The compound is the most common long-chain saturated fatty acid that is naturally produced by the wide range of plants, animals, and microorganisms. The compound was found in the literature to possess vast bioactivity such as antibacterial, anti-inflammatory, anti-fungal [2, 17], antioxidant [23], pesticide, antioxidant, hy-pocholesterolemic nematicide and 5-Alpha-reductase inhibitor [17, 24]. Furthermore, hexa-

decanoic acid was recently reported to poses cytotoxic activities against cancer cell lines [5, 17, 26]. Thus, the effective anti-proliferative activities demonstrated by the selected crude extracts in this study might be contributed by the synergistic effect of this compound with the other compounds identified. Palmic acid and triacetin was present both in methanol (1.35 %) and (0.35) and, ethyl acetate extracts (1.65 %) and (1.08) respectively. These biological activities of compounds present in A. altilis leaf extract support the medicinal application of the plant. The study revealed major bioactive compounds present in all of the extracts. Identification of these compounds in the plant serves as the basis in determining the possible health benefits of the plant leading to further biologic and pharma-cologic studies.

In addition, naturally occurring vitamin E found naturally in some foods was also found in methanol extract. Vitamin E exists in eight chemical forms (alpha-, beta-, gamma-, and delta-tocopherol and alpha-, beta-, gamma-, and delta-tocotrienol) that have varying levels of biological activity [25]. Vitamin E is a fat-soluble antioxidant that stops the production of ROS formed when fat undergoes oxidation. Despite its activi-

ties as an antioxidant, vitamin E is involved in immune function and, as shown primarily by in vitro studies of cells, cell signalling, regulation of gene expression, and other metabolic processes [29, 40], it was reported that vitamin C-induced cell death as observed in many cancer cells [39].

CONCLUSIONS

The two extracts possess major bioactive compounds that were identified and characterized spectroscopically. Thus, identification of different biologically active compounds in the extracts of A. altilis leaves warrants further biological and pharmacological studies.

ACKNOWLEDGMENTS

This study was supported by Universiti Tun Hussein Onn Malaysia under GPPS grant (No 608) by Office for Research, Innovation, Commercialization and Consultancy.

CONFLICT OF INTEREST STATEMENT

We declare that we have no conflict of interest.

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