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Науковий в^ник Львiвського нащонального унiверситету ветеринарно! медицини та бiотехнологiй iMeHi С.З. Гжицького. CepiH: Сiльськогосподарськi науки
Scientific Messenger of Lviv National University of Veterinary Medicine and Biotechnologies. Series: Agricultural sciences
ISSN 2519-2698 print
https://nvlvet.com.ua/index.php/agriculture_doi: 10.32718/nvlvet-a9011
UDC 636.52
The effect of Chlorella suspension on productivity of chickens
K.S. Garbazhii1, O.J. Karunskii2
'Odessa National Academy of Food Technologies, Odessa, Ukraine 2Odessa Agrarian State University, Odessa, Ukraine
Garbazhii, K.S., & Karunskii, O.J. (2019). The effect of Chlorella suspension on productivity of chickens. Scientific Messenger of Lviv National University of Veterinary Medicine and Biotechnologies. Series: Agricultural sciences, 21(90), 63-67. doi: 10.32718/nvlvet-a9011
The full feeding is one of the most important conditions that form the level of productivity in poultry industry. The value of feeding rations is known to depend not only on the presence of all essential substances in them, but also on the degree of its biological availability. Due to a wide range of vitamins, including fat-soluble, as well as macro- and micro elements in a biologically accessible form in Chlorella composition, Chlorella suspension can be become the useful component in feeding of poultry farming. Therefore, the aim of our study was to investigate the influence of the Chlorella water suspension on growth, daily weight increment and amount of eggs of chicken. The Chlorella suspension was produced by "Samvel Farm Enterprise" in Bilyaivka district of Odessa region, Ukraine. The investigation was carried out on layer chickens of "Brown" breed at the age of 360 days, which were divided into 3 experimental groups: one of them was control group and two were research groups with 300 heads of layer chickens in each. Despite of Chlorella suspension chickens were fed by ordinary animal feeding stuff. Most of the feed composition for all chicken groups consisted of wheat, barley and limestone. The nutritional value of this complete feed involved the presence of the crude protein, crude fiber and necessary amino acids including lysine, methionine. The second chicken group, throughout the experiment during '60 days was fed with a suspension of Chlorella in a concentration of 50 million, and in the 3d experimental group - 60 million cells in 1 milliliter of fluid. The results of research showed that the use of Chlorella suspension positively influences the raise of growth of chickens in the 2nd and 3rd experimental groups, which is characterized by the higher absolute, relative and average daily increments. The maximum result was achieved in 2nd experimental group, where the optimal cell concentration in the Chlorella suspension was 50 million cells in 1 milliliter of fluid and daily rate of increment was 30 grams per 1 head. Also after feeding with Chlorella suspension egg-laying increased by 12.4% compared to egg-laying of chickens in control group. Consequently, the inclusion of Chlorella suspension in chicken feeding allows obtaining poultry products with the maximum consumer qualities.
Key words: feeding, layer chickens, suspension, Chlorella, productivity.
Вплив суспензи хлорели на продуктившсть курей
К.С. Гарбажий1, О.Й. Карунський2
'Одеська нацюналъна академiя харчових технологш, м. Одеса, Украша 2Одеський аграрний державний yHieepcumem, м. Одеса, Украша
Повноцтне годування вiдноситъся до числа найважливШих умов, що формують рiвенъ nродуктивностi в тваринництви Вiдо-мо, що значення кормовихращотв залежитъ не ттъки вiд наявностi в них уЫх необхiднихречовин, але i вiд ступеня гх бiологiчноi доступностi. Завдяки широкому спектру вiтамiнiв, включаючи жиророзчинт, а також макро- та мтроелементи в бюлог1чно доступтй формi у складi хлорели, суспензiя хлорели може стати важливим компонентом у годiвлi птищ. Тому метою нашого до^дження було вивчення впливу водног суспензи Хлорели на ркт, добовий приркт ваги та ктъкктъ яецъ курей. Суспензiя Хлорели була виготовлена компатею "Самвел Фермерсъке тдприемство " у Бтяг'всъкому район Одесъког области Украгна. До^дження проводили на курях породи "Браун " у вц 360 дмв, як були роздтеш на 3 експерименталъш групи: одна з них була контролъною
Article info
Received 12.02.2019 Received in revised form
13.03.2019 Accepted 14.03.2019
Odessa National Academy of Food Technologies, Kanatna Str., 112, Odessa, 65000, Ukraine. Tel.: +38(067)-466-14-72 E-mail: garbazhiykat@gmail.com
Odessa Agrarian State University, Pantelijmonivska Str., 13, Odessa, 65000, Ukraine.
групою, двi - достдш групи по 300 голiв курей. Окрм суспензИХлорели, курей годували звичайним кормом для тварин. Пшениця, ячмть i вапняк складали найбтьшу частину корму для вых груп курей. Поживна цттсть цього корму полягала у наявностi сирого протегну, сирог клШковини i необхiдних амтокислот, включаючи лiзин, метюнт. Другу групу курей протягом всього експерименту протягом 160 дтв, годували суспензшю хлорели в концентрацИ 50 мтьйотв, а 3-ю до^дну - в 60 мтьйошв клтин в 1 мл рiдини. Результати до^джень показали, що застосування суспензИ Хлорели позитивно впливае на тдвищення росту курей у 2-й i 3-й до^дних групах, що характеризуется вищим абсолютним, вiдносним i середньодобовим приростом. Максимальний результат був досягнутий у 2-й до^днш груп1 де оптимальна концентращя клШин в суспензИ Хлорели становила 50 мтьйотв клШин в 1 мл рiдини i добова швидмсть приросту становила 30 грамiв на 1 голову. Також тсля годiвлi суспензiею Хлорели яйцекладка збтьши-лася на 12,4% в порiвняннi з яйцекладкою курей контрольног групи. Отже, включення суспензИ хлорели в годiвлю курей дозволяе отримувати продукти птахiвництва з максимальними споживчими якостями.
Ключовi слова: годiвля, кури-несучки, суспензiя, Хлорела, продуктивтсть.
Introduction
The use of biologically active additives (BAA) in feed production is fundamentally important. In turn, the effectiveness of using BAA itself depends on their composition and concentration of biologically active substances in them, their form, digestibility, origin, production technologies, etc (Yun & Park, 2001; Skfivan et al., 2009). One of the ways to increase the productivity and safety of poultry is using of Chlorella in feeding, which allows to improve the quality and biological safety of marketable products by reducing the amount of synthetic additives (Doucha et al., 2009; Liang et al., 2009; An et al., 2016). Notable among the biologically active additives is the Chlorella microalgae (Chlorella Vulgaris), composing of a significant amount of protein, a wide range of vitamins, including fat-soluble, as well as macro- and micro elements in a biologically accessible form (de-Bashan et al., 2002; Heindl et al., 2010; Kang, et al., 2017). In the course of its lifetime, microalgae synthesize substances that possess both bacteriostatic and probiotic effects. In recent years, as part of the practical use of Chlorella, production experiments have been carried out in various livestock farming sectors with positive results compared with other feed additives (Watanabe et al., 1955; Widjaja et al., 2009). These results indicate that there is no alternative to Chlorella as a feed additive in animal husbandry (Watanabe et al., 1955; Sevcikova et al., 2006; Kang et al., 2013).
The benefit of this product is possibility to be cultivated throughout the year. The level of technology, the quality of raw materials and other factors determine the biological value of the final product. But the main reason is the impossibility of using live natural plants in a dry matter (Huang et al., 2010). It is its inclusion as a source of high biological value remains a large reserve for poultry farming today. The usage of suspensions helps not only significantly increase the productivity of the poultry and improve the quality of products, but also increase the profitability of the industry in general.
Technological peculiarities of the use of Chlorella biomass is possible to be introduced into the diet, both in the form of a paste, and into feed, and in the form of a suspension. Chlorella concentrate is a new fodder product of natural origin, which includes the higher complex of biologically active substances (Halle & Janczyk, 2009). Unlike paste, the suspension has biologically active products of Chlorella's secondary metabolism, but it has a low
dry matter content and a low expiration date (Ogbonna et al., 1997).
The unique biological composition, large energy capacity, antioxidant properties and the ability to stimulate the activity of the immune system put this valuable microscopic organism of plant origin in the category of true natural healing remedies, synthesized Chlorella substances. This makes it easy to obtain products of a functional purpose, for example, enriching of essential microelements, poultry meat and eggs by changing the composition of the nutrient medium (Surai, 2002; Janczyk et al., 2007; Skfivan et al., 2008; Zhu et al., 2013; Kotrbacek et al., 2015). This fact alone in combination with high ecological purity of products has great importance for human health (Janczyk et al., 2005; Doucha et al., 2006). Also the production of Chlorella suspension has no waste, because it is used as animal feed (Kotrbacek et al., 2013). Despite the clear benefits of using Chlorella as a feed additive, currently there are few publications devoted to the influence of Chlorella's suspension on poultry productivity and its product quality.
The aim of the work was to study the influence of the Chlorella water suspension as a part of complete feeding for chickens on changing of their live weight at different stages of feeding, productivity.
Materials and methods
The breed chickens "Brown" were used in this research. The study of the use of Chlorella suspension was carried out at the poultry plant "Samvel Farm Enterprise" in Bilyaivka District of Odessa region. The feed updates occurred daily.
Materials. The objects of research were Brown breed chickens at the age of 365 days and 3 experimental groups were formed: 1 - control group and 2 and 3 were experimental groups, 300 head in each group according to the principle of analogues with a concentration of 50 and 60 million cells per 1 milliliter respectively (Table 1).
For the preparation of the feed additive, the planktonic strain Chlorella vulgaris IGF No. C-111 was used, which is distinguished by a high degree of use of light energy (efficiency of photosynthetically active radiation of 3.6%) and the chemical composition of the cell according to the content of proteins, essential amino acids, vitamins, and biologically active substances. Algae were reproduced into the aquarium for a month.
Table 1
Scheme of research
Groups of the poultry Number of chicken in each group, heads Variant of feed Duration of the research, days
1 - control group 2 - research group 3 - research group 300 300 300 Complete feed (CF) CF + Chlorella suspension with a concentration of 50 million cells per 1 milliliter CF + Chlorella suspension with a concentration of 60 million cells per 1 milliliter 160 160 160
The composition and nutritional value of complete feed used in feeding for the experimental groups are presented in Tables 2 and 3 respectively. The Chlorella suspension was added in the drinking water of the 2nd and 3rd research groups, at a concentration of 50 and 60 million cells per 1 milliliter of culture fluid. Cells concentration in suspension of Chlorella was diluted with tap water.
Table 2
Composition of complete feed for experimental groups of chicken, %
Constituents % Constituents %
Corn - Baking soda -
Wheat 52.69 Limestone 9.00
Barley 14.20 Soya bean oil -
Sunflower oil cake 7.00 Vitamin-mineral premix 0.50
Soybean cake 5.23 Mono-calcium phosphate 0.90
Fishmeal 4.46 Lysine 0.50
Wheat bran 5.02 Kreoline 0.10
Table salt 0.25 Methionine 0.15
Total 100
Table 3
Nutritional value of complete feed for experimental groups of chicken, %
Constituents %
Available energy 1.10
Crude protein 12.50
Crude fiber 2.50
Lysine 0.05
Methionine 0.80
Tryptophan 0.40
Calcium 3.50
Phosphorus 0.70
Sodium 0.20
Methods. The main indicators of assessing the effectiveness of Chlorella suspension using were the dynamics
of changes in poultry live weight and feed costs by 1 kg of growth. Live weights, absolute increment, average daily weight increment were determined by weight equipment after 445 and 525 day in all groups of chickens. The chicken weighing was conducted 2 times per day, especially in the morning and in the evening.
Statistical analysis. Reliability of the data received was determined by t-criterion using Microsoft Excel 2007 (Microsoft Co., USA). In order to present data ANOVA as well as Fisher's LSD post-hoc test were performed using XL STAT (Addinsoft, Paris, France).
Results and discussion
The poultry in all the groups were fed by the basic diet, energy and nutritional value conformed to generally accepted norms. In two research groups, the increment of weight in chickens from the beginning of the research and until the end of the research was higher in relation to the control group; the largest increment was especially determined in the 2nd experimental group. As for the ratio of the amount of feed per one kilogram of weight increment, the rates of in all groups were almost equal, but lower than the control group by 6.2%. The results are shown in Table 4.
The use of Chlorella suspension as a part of basic feeding significantly increased the average daily weight of chickens. According to ANOVA results, the chickens live weight of research groups are differed depending to poultry age and concentrations of Chlorella suspension utilizing for feeding (Table 4). The obvious fact is that average daily weight has increased every day but in the second group on day 525 there was no noticeable difference after 80 days of feeding.
The pattern of poultry growth was estimated on the basis of absolute and daily average increments (Table 5). Based on the results, the relative increment in the first group of chickens did not change during 525 days.
Table 4
Dynamics of the chickens live weight of research groups
Poultry age, days_1 - control group, grams_2 - research group, grams_3 - research group, grams
365 1805a 1800a 1800a 445 1887b 1998b 1896b 525 1980c 1993bc 1990c p-significance_***_**_**_
Table 5
Indices of increment of live weight of the poultry in experimental groups
Parameters
Group Age, days Absolute increment, grams Average daily weight increment, grams Relative increment, %
365 - - -
1 - control group 445 82.0a 1.0a 100
525 80.0a 1.0a 100
365 - - -
2 - - research group 445 98.0b 1.2b 119.5
525 95a 1.1ab 118.7
365 - - -
3 - - research group 445 96.0b 1.2b 117.0
525 94.0ab 1.1ab 117.5
_p-significance_
1 - control group ns ns -
2 - research group ** * -
_3 - research group_**_*_-_
Each value is based on n = 300. Means separated at P < 0.05 by Fisher's Least Significant Difference. Means within columns followed by different letters are significantly different, ns - no significant
Absolute increment and average daily weight increment of first chicken research group were not changed according to age (Table 5). The same indictors of the second and third research groups were decreasing with duration of days.
The main products of poultry farming are eggs and meat. Poultry lay eggs which vary in size. The egg mass determines the overall presence of yolk and protein in them and it is one of the main indicators for classification according to the standard.
The poultry of the control group gave 20181 eggs, accounting for 69% egg-laying. The productivity of the poultry of the research group amounted to 79.4% (23820 eggs were obtained), which is 12.4% higher than in the control group. The weight of the poultry's egg in the control group was 63%, while in the experimental group it was 69.2%, which is by 5.5 grams or by 8.6% higher. According to these parameters, the egg mass in the control group was 128 kg, in the experimental group the egg mass was 165 kg, which is by 37 kg higher with an average egg weight of 69.2 g.
The possibility of providing of chicken with a natural plant of such biological value opens up to the poultry industry without exaggeration the widest prospects and up to two times the profitability of the products produced. Also it allows obtaining poultry products at the maximum consumer qualities, both in terms of taste and environmental indicators that will lead to increase the popularity and consumption of poultry products in general by the population, and will allow increasing large-scale production volumes.
Conclusions
Summing up the analysis of study, we conclude the following statements:
1. Using Chlorella suspension it is possible to increase the main indicators of chicken productivity during the year.
2. The use of Chlorella suspension in the feeding for layer chickens in the dose of 30 ml/head with a
concentration of 50 million cells per 1 milliliter of solution positively affects chicken growing quicker, as indicated by the higher absolute, relative and average daily increments in experimental groups compared to control group.
3. Feeding with Chlorella suspension also improves the quality of obtained products from chickens, especially the mass of eggs and their number.
References
An, B.-K., Kim, K.-E., Jeon, J.-Y., & Lee, K.W. (2016). Effect of dried Chlorella vulgaris and Chlorella growth factor on growth performance, meat qualities and humoral immune responses in broiler chickens. Spr Pl, 5(1), 718. doi: 10.1186/s40064-016-2373-4. de-Bashan, L.E., Moreno, M., Hernandez, J.-P., & Bashan, Y. (2002). Removal of ammonium and phosphorus ions from synthetic wastewater by the microalgae Chlorella vulgaris coimmobilized in alginate beads with the microalgae growth-promoting bacterium Azospirillum brasilense. Water Res, 36(12), 2941-2948. doi: 10.1016/S0043-1354(01)00522-X. Skfivan, M., Simanë, J., Dlouha, G., & Doucha, J. (2006). Effect of dietary sodium selenite, Se-enriched yeast and Se-enriched Chlorella on egg Se concentration, physical parameters of eggs and laying hen production. Czech J Anim Sci, 51(4), 163-167. doi: 10.17221/3924-CJAS. Doucha, J., Livansky, K., Kotrbacek, V., & Zachleder, V. (2009). Production of Chlorella biomass enriched by selenium and its use in animal nutrition: A review. Appl Microbiol Biotechnol, 83(6), 1001-1008. doi: 10.1007/s00253-009-2058-9. Halle, I., & Janczyk, P. (2009). Effect of microalgae Chlorella vulgaris on laying hen performance. Arch Zootech, 12(2), 5-13. Heindl, J., Ledvinka, Z., Englmaierova, M., Zita, L., & Tumova, E. (2010). The effect of dietary selenium sources and levels on performance, selenium content in muscle and glutathione peroxidase activity in
broiler chickens. Czech J Anim Sci, 55(12), 572-578. doi: 10.17221/2487-CJAS.
Huang, G., Chen, F., Wei, D., Zhang, X., & Chen, G. (2010). Biodiesel production by microalgal biotechnology. Appl Ener, 87(1), 38-46. doi: 10.1016/j.apenergy.2009.06.016.
Janczyk, P., Franke, H., & Souffrant, W.B. (2007). Nutritional value of Chlorella vulgaris: Effects of ultrasonication and electroporation on digestibility in rats. Anim Feed Sci Technol, 132(1-2), 163-169. doi: 10.1016/J.ANIFEEDSCI.2006.03.007.
Janczyk, P., Wolf, C., & Souffrant, W.B. (2005). Evaluation of nutritional value and safety of the green micro-algae Chlorella vulgaris treated with novel processing methods. Arch Zootechn, 8, 132-147.
Kang, H.K., Park, S.B., & Kim, C.H. (2017). Effects of dietary supplementation with a chlorella by-product on the growth performance, immune response, intestinal microflora and intestinal mucosal morphology in broiler chickens. J Anim Physiol Anim Nutr, 101(2), 208-214. doi: 10.1111/jpn.12566.
Kang, H.K., Salim, H.M., Akter, N., Kim, D.W., Kim, J.H., Bang, H.T., & Suh, O.S. (2013). Effect of various forms of dietary Chlorella supplementation on growth performance, immune characteristics, and intestinal microflora population of broiler chickens. J Appl Poul Res, 22(1), 100-108. doi: 10.3382/japr.2012-00622.
Kotrbacek, V., Doubek, J., & Doucha, J. (2015). The chlorococcalean alga Chlorella in animal nutrition: a review. J Appl Phycol, 27(6), 2173-2180. doi: 10.1007/s10811-014-0516-y.
Kotrbacek, V., Skfivan, M., & Kopecky, J. (2013). Retention of carotenoids in egg yolks of laying hens supplemented with heterotrophic Chlorella. Czech J Anim Sci, 58(5), 193-200. doi: 10.17221/6747-CJAS.
Liang, Y., Sarkany, N., & Cui, Y. (2009). Biomass and lipid productivities of Chlorella vulgaris under
autotrophic, heterotrophic and mixotrophic growth conditions. Biotechnol Lett, 31(7), 1043-1049. doi: 10.1007/s10529-009-9975-7.
Ogbonna, J.C., Masui, H., & Tanaka, H. (1997). Sequential heterotrophic/autotrophic cultivation - An efficient method of producing Chlorella biomass for health food and animal feed. J Appl Phycol, 9(4), 359-366. doi: 10.1023/A:1007981930676.
Sevcikova, S., Skrivan, M., Dlouha, G., & Koucky, M. (2006). The effect of selenium source on the performance and meat quality of broiler chickens. Czech J Anim Sci, 51(10), 449-457. doi: 10.17221/3964-CJAS.
Skfivan, M., Marounek, M., Dlouha, G., & Sevcikova, S. (2008). Dietary selenium increases vitamin E contents of egg yolk and chicken meat. British Poul Sci, 49(4), 482-486. doi: 10.1080/00071660802236021.
Surai, P.F. (2002). Selenium in poultry nutrition 2. Reproduction, egg and meat quality and practical applications. World's Poult Sci J, 58(04), 431-450. doi: 10.1079/WPS20020032.
Watanabe, A., Ito, R., & Sasa, T. (1955). Micro-Algae as a source of nutrients for daphnids. J Gen Appl Microbiol, 1(2), 137-141. doi: 10.2323/jgam.1.137.
Widjaja A., Chien C.-C., & Ju, Y.-H. (2009). Study of increasing lipid production from fresh water microalgae Chlorella vulgaris. J Taiwan Inst Chem Eng, 40(1), 1320. doi: 10.1016/J.JTICE.2008.07.007.
Yun, Y.-S., & Park, J. (2001). Attenuation of monochromatic and polychromatic lights in Chlorella vulgaris suspensions. Appl Microbiol Biotechnol, 55(6), 765-770, doi: 10.1007/s002530100639.
Zhu, L., Wang, Z., Shu, Q., Takala, J., Hiltunen, E., Feng, P., & Yuan, Z. (2013). Nutrient removal and biodiesel production by integration of freshwater algae cultivation with piggery wastewater treatment. Water Res, 47(13), 4294-4302, doi: 10.1016/J.WATRES.2013.05.004
