Figure 1: Photoacoustic imaging of microvessels at different organ sites
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Figure 2: Photoacoustic imaging of calf gastrocnemius region offive human volunteers, M: male, F:
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REFERENCES
[1] Ningbo Chen, Jia Yu, Liangjian Liu, Zhiqiang Xu, Rongkang Gao, Tao Chen, Liang Song, Wei Zheng*, Chengbo Liu, "Video-rate high-resolution single-pixel nonscanning photoacoustic microscopy, Biomedical Optics Express. 3823-3835, 2022.
[2] Zhiqiang Xu, Yinhao Pan, Ningbo Chen, Silue Zeng, Liangjian Liu, Rongkang Gao, Jianhui Zhang, Chihua Fang, Liang Song, Chengbo Liu, "Visualizing tumor angiogenesis and boundary with polygon-scanning multiscale photoacoustic microscopy, Photoacoustics. 100342, 2022.
[3] Tao Chen, Liangjian Liu, Xiaohui Ma, Ying Zhang, Huadong Liu, Rongqin Zheng, Jie Ren, Huichao Zhou, Yaguang Ren, Rongkang Gao, Ningbo Chen, Hairong Zheng, Liang Song, Chengbo Liu, Dedicated photoacoustic imaging instrument for human periphery blood vessels: A new paradigm for understanding the vascular health, IEEE Transactions on Biomedical Engineering. 1093-1100, 2022.
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[5] Huangxuan Zhao, Ningbo Chen, Tan Li, Jianhui Zhang, Riqiang Lin, Xiaojing Gong, Liang Song, Kunya Zhang, Zhi Yang, Zhicheng Liu, Chengbo Liu, Motion Correction in Optical Resolution Photoacoustic Microscopy, IEEE Transactions on Medical Imaging. 2139-2150, 2019.
DOI 10.24412/cl-37136-2023-1-131-133
THROUGH-INTACT-SKULL WINDOW: A CHRONIC SKULL OPTICAL CLEARING WINDOW FOR LONG-TERM CORTICAL OBSERVATION
DONGYU LI1,2, ZHENGWU HU1, WANG XI4, JUN QIAN3, DAN ZHU1*
1 Britton Chance Center for Biomedical Photonics - MoE Key Laboratory for Biomedical Photonics, Wuhan National Laboratory for Optoelectronics, Huazhong University of Science and Technology,
Wuhan, Hubei 430074, China.
2 School of Optical Electronic Information, Huazhong University of Science and Technology, Wuhan,
Hubei 430074, China.
3 State Key Laboratory of Modern Optical Instrumentations, Centre for Optical and Electromagnetic
Research, College of Optical Science and Engineering, International Research Center for Advanced
Photonics, Zhejiang University, Hangzhou 310058, China. 4 Interdisciplinary Institute of Neuroscience and Technology, Department of Anesthesiology, the Second Affiliated Hospital, School of Medicine, Zhejiang University, Kaixuan Road 258th, Hangzhou, 310020,
PR China.
li_dongyu@hust.edu.cn
ABSTRACT
Modern optical imaging techniques provide powerful tools for observing cortical structure and functions at high resolutions. Various skull windows have been established for different applications of cortical imaging, and each has its advantages and limitations. Most critical of the limitations, none of the current skull windows is suitable for observing the responses to some acute craniocerebral injuries on a large scale and at high resolution.
In order to develop a skull window technique capable of simultaneously meeting requirements, including maintenance of the internal environment and provision of a wide field of view, high resolution, and durability, we developed a chronic optical clearing window skull, named "Through-Intact-Skull (TIS) window" by firstly removing the collagens and lipids in skull, and secondly stabilizing its transparent state using an ultraviolet curing adhesive. Fig.1 and 2 shows the performance of TIS window.
TIS window enables the observation of an immune response on a bilateral cortical scale and at single-cell resolution after traumatic brain injury without affecting the pathological environment of the brain. What we have observed in this research, including simultaneously discerning the movements of immune cells in different regions and perceiving random hemorrhages that occur in the bilateral cerebral cortex after acute traumatic brain injury (TBI), could not be achieved before now using previous skull window techniques. In addition, the TIS window also has the advantages of craniotomy-freeness, centimeter-field of view, synaptic resolution, large imaging depth, long-term observation capability, and suitability for awake mice.
Figure 1: Large-scale high-resolution cortical neurovascular imaging through TIS window.
Figure 2: Three-photon fluorescence imaging of deep=cortical neurons through TIS window.
Therefore, the TIS window is a promising new approach for intravital cortical microscopy in basic research in neuroscience. The craniotomy-free TIS window with easy and quick steps, as a chronic skull optical clearing window, has advantages over the open-skull glass window, thinned skull window, "transparent skull" window, and skull optical clearing window, and is capable of continuous cortical monitoring at high resolution and on a centimeter-scale, with minimized influence on the brain environment. Therefore, the TIS window technique holds great potential for physiological and pathological research in brain science.