CC BY
4The 30th International Conference on Advanced Laser Technologies
ALT'23
B-I-12
Tissue optical clearing imaging: from in vitro to in vivo
Dan Zhu
Britton Chance Center for Biomedical Photonics - MoE Key Laboratory for Biomedical Photonics, Advanced Biomedical Imaging Facility-Wuhan National Laboratory for Optoelectronics, Huazhong University of Science and Technology, Wuhan, Hubei 430074, China. [email protected]
Biomedical optical imaging techniques provide powerful tools for observing biomedical tissue structural and functional information. However, the high scattering of biological tissues limits the penetration of light, and decreases imaging resolution and contrast as light propagates deeper into the tissue. Fortunately, novel tissue optical clearing technique provide a way for solving the above problem. This presentation will introduce some progress in tissue optical clearing imaging, i.e., in vitro tissue optical clearing methods for whole organs imaging; in vivo skull/skin optical clearing window for imaging structural and functional of cutaneous / cortical vascular and cells.
[1] Y. Qi, T. Yu, J. Xu, P. Wan, Y. Ma, J. Zhu, Y. Li, H. Gong, Q. Luo, and D. Zhu, FDISCO: advanced solvent-based clearing method for imaging whole organs,Science Advances, 5(1), eaau8355 (2019).
[2] J. Zhu , T. Yu , Y. Li, J. Xu, Y. Qi, Y. Yao, Y., P. Wan, Z. Chen, X. Li, H. Gong, Q. Luo, and D. Zhu, MACS: Rapid aqueous clearing system for three-dimensional mapping of intact organs, Advanced Science, 201903185 (2020).
[3] C. Fang, T. Yu, T. Chu, W. Feng, F. Zhao, X. Wang, Y. Huang, Y. Li, P. Wan, W. Mei, D. Zhu, and P. Fei, Minutes-timescale 3D isotropic imaging of entire organs at subcellular resolution by content-aware compressed-sensing light-sheet microscopy, Nature Communications, 12(1):107 (2021).
[4] J. Xu, Y. Li, Y. Yao, A. Xuan, J. Zhu, D. Li, T. Yu, and D. Zhu, Three-dimensional mapping reveals heterochronic development of the neuromuscular system in postnatal skeletal muscles. Communication Biology, 5(1): 1200 (2022).
[5] D. Li, Z. Hu, H. Zhang, Q. Yang, L. Zhu, Y. Liu, T. Yu, J. Zhu, J. Wu, J. He, P. Fei, Wang Xi, Jun Qian, and D. Zhu, A Through-Intact-Skull (TIS) chronic window technique for cortical structure and function observation in mice, eLight, 2:15 (2022).
[6] Y. Zhao, T. Yu, C. Zhang, Z. Li, Q. Luo, T. Xu, and D. Zhu, Skull optical clearing window for in vivo imaging of the mouse cortex at synaptic resolution, Light: Science & Applications, 7, 17153 (2018).
[7] C. Zhang, W. Feng, Y. Zhao, T. Yu, P. Li, T. Xu, Q. Luo, and D. Zhu, A large, switchable optical clearing skull window for cerebrovascular imaging, Theranostics, 8(10): 2696-2708 (2018).
