CC BY
7TISSUE OPTICAL CLEARING WINDOWS FOR IN VIVO IMAGING
DAN ZHU12
1 Britton Chance Center for Biomedical Photonics, Wuhan National Laboratory for Optoelectronics, Huazhong University ofScience
and Technology, China
2 MoE Key Laboratory for Biomedical Photonics, Huazhong University of Science and Technology, China
ABSTRACT
Biomedical photonics is currently one of the fastest growing fields of life sciences since optical imaging techniques allow low-invasive in vivo structural and functional analysis of tissues with high resolution and contrast unattainable by any other method (1-2). However, the high scattering of turbid biological tissues limits the penetration of light, leading to strongly decreased imaging resolution and contrast as light propagates deeper into the tissue. For instance, observation of brain structure and neural activities is of great significance to understand not only normal brain physiology but also dysfunctions of vasculature and neural networks related to various brain diseases (3-4), while the skull above prevent in vivo optical brain imaging. Moreover, as the largest organ of the body, skin is an ideal target tissue for microvascular network and immune response monitoring, but its turbid nature severely limits the visualization by decreasing the imaging resolution as well as the imaging depth. Traditionally, to overcome the scattering of such barriers above the target tissue, surgery-based windows have to be performed. Fortunately, novel in vivo tissue optical clearing technique could reduce the scattering of tissue and make it transparent for higher optical imaging quality. This presentation will introduce the recently developed skin/skull optical clearing windows for imaging structure and function of cutaneous/cortical vascular and cells, as well as for manipulating cortical vasculature (5-8).
Figure: Different optical clearing windows for improving cortical neuro/vascular imaging depth [4-8]
REFERENCES
[1] N. Wagner et al., "Instantaneous isotropic volumetric imaging of fast biological processes," Nat Methods 16(6), 497 (2019).
[2] R. Prevedel et al., "Fast volumetric calcium imaging across multiple cortical layers using sculpted light," Nat Methods 13(12), 1021-1028 (2016).
[3] P. McGonigle, "Animal models of CNS disorders," Biochem. Pharmacol. 87(1), 140-149 (2014).
[4] M. Wiesmann et al., "A specific dietary intervention to restore brain structure and function after ischemic stroke," Theranostics 7(2), 493-512 (2017).
[5] Y. J. Zhao et al., "Skull optical clearing window for in vivo imaging of the mouse cortex at synaptic resolution," Light-Sci Appl 7(2), 17153 (2018).
[6] C. Zhang et al., "Age differences in photodynamic therapy-mediated opening of the blood-brain barrier through the optical clearing skull window in mice," Laser Surg Med 51(7), 625-633 (2019).
[7] D. Li, et al., Visible-near infrared-II skull optical clearing window for in vivo cortical vasculature imaging and targeted manipulation, Journal of Biophotonics, e202000142, 2020
[8] Y. Chen, Coherent Raman Scattering Unravelling Mechanisms Underlying Skull Optical Clearing for Through-Skull Brain Imaging, Analytical Chemistry, 91, 9371-9375, 2019
