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Scientific Messenger of Lviv National University of Veterinary Medicine and Biotechnologies
ISSN 2518-7554 print doi: 10.32718/nvlvet8822
ISSN 2518-1327 online http://nvlvet.com.ua
UDC 636.4.084.1:543-414
The efficiency of using of Alfasorb in piglets feeding
O.P. Reshetnychenko1, O.T. Piven1, E.U. Rozum1, V.I. Savshenko1, A.I. Levinsky1, A.V. Kovalenko2, V.V. Voronyak3
'Odessa State Agrarian University, Odessa, Ukraine 2LLCSPE "ARIADNA", Odessa, Ukraine
3Stepan Gzhytskyi National University of Veterinary Medicine and Biotechnologies Lviv, Ukraine
Reshetnychenko, O.P., Piven, O.T., Rozum, E.U., Savshenko, V.I., Levinsky, A.I., Kovalenko, A.V., & Voronyak, V.V. (2018). The efficiency of using of Alfasorb in piglets feeding. Scientific Messenger of Lviv National University of Veterinary Medicine and Biotechnologies, 20(88), 120124. doi: 10.32718/nvlvet8822
Mycotoxins cause severe diseases in organisms of human and animals. They are difficult to diagnose, cause great economic damage. Sorbents are able to effectively bind and remove from the body through the digestive tract with a therapeutic or prophylactic purpose various endogenous and ekzogenous substances, microorganisms and they toxins, supermolecular structures and cells. The aim of the work is to study the effectiveness of use of Alfasorb for the neutralization of mixed fodders affected by mycotoxins and they impact on blood's indexes and productive qualities of piglets of Large White Breed. Experience was conducted on 30 ty piglets. Animals of the control group received mixed feed that was produced in the conditions of the farm. The pigs of experimental group in addition to the basic diet received Alfasorb. We determined blood's indexes for study the effect of feed additive Alfasorb on the physiological state of animals at 4 months of age. A morphological and histological study of liver was carried out. The analysis of the average daily weight gain of piglets showed that it was higher in period of growth in the animals of experimental group. In the animals of the experimental groups there was an increase in the number of red blood cells in comparison with the control. An unreliable decrease in blood serum levels which characterize the level of intermediate metabolism of proteins in the body (content of urea, creatinine and common bilirubin) was established in the animals of the experimental group as compared to the control group respectively by 22.83% (td = 0.98, P < 0.05), 7.'2% (td = '.92, P < 0.05) and by 20.54% (td = 0.86, P < 0.05). Most hepatocytes had large nucleus and intense color of the cytoplasm. This indicates the absence of alterative processes in the body. The results of the studies make it possible to assert that the inclusion the 0.2% Alfasorb to the compound of feed which contaminated with mycotoxins significantly reduces their negative effect on the piglets organism, has a positive effect on the growth rate, on the course of metabolic processes, also it promotes an increase in the liver of medium-sized hepatocyte nuclei, stimulates biosynthetic processes in that cells and ensures the formation of a full structure of histohematological barriers in the organism.
Key words: Alfasorb, blood's indexes, living weight, liver, mycotoxines, piglets.
Ефектившсть застосування альфасорбу у годiвлi поросят
О.П. Решетшченко1, О.Т. Ивень1, С.Ю. Розум1, ВХСавченко1, А.1. Левшський1, А.В. Коваленко2, В.В. Вороняк3
'Одеський державний аграрний утверситет, м. Одеса, Украша 2ОАТНПП "Арiадна", м. Одеса, Украша
3Львiвський нацюнальний утверситет ветеринарноймедицини та бютехнологт iменi С.З. Гжицького, м. Львiв, Украша
Мжотоксини спричиняють fi3m захворювання в органiзмi людини та тварин. Вони важко дiагностуються та призво-дять до великих економiчних збитюв. Сорбенти здатт ефективно зв'язувати та виводити з оргашзму через травний тракт з профмактичною чи терапевтичною метою рiзнi ендогенш та екзогенш субстанцп, мжрооргашзми та гх токсини,
Article info
Received 04.09.2018 Received in revised_ form 02.10.2018 Accepted 03.10.2018
Odessa State Agrarian University, Panteleymonyvska, 13, Odessa 65012, Ukraine. Tel.: +38-048-784-57-32 E-mail: ogsi@te.net.ua
LLC SPE "ARIADNA ", Motornaia Str., 8a, Odessa, 65085, Ukraine.
Stepan Gzhytskyi National University of Veterinary Medicine and Biotechnologies Lviv, Pekarska Str., 50, Lviv, 79010, Ukraine.
складт молекулярт структури та клтини. Мета роботи - до^дити ефективтсть застосування альфасорбу для нейт-ралiзацií комбiкормiв, уражених мжотоксинами, та гх вплив на показники кровi та продуктивт якостi поросят великог быог породи. До^д проводили на 30-ти поросятах. Тварини контрольног групи отримували комбжорм, що виробляеться в умовах ферми. Поросята до^дног групи у додаток до основного ращону одержували альфасорб. Ми вивчали показники кровi для визначення впливу кормовог добавки альфасорб та фiзiологiчний стан тварин 4^сячного вi^. Було проведено морфологiчне i гiстологiчне до^дження печiнки. Аналiз середньодобових приростiв поросят показав, що вони були быь-шими у перiод росту тварин експериментальног групи. У тварин до^дног групи була быьшою кыьтсть еритроцитiв, у порiвнянш з контролем. Встановлено зниження у сироватц кровi тварин до^дног групи показниюв, що характеризують промiжний обмт (вмкт сечовини, креатинту та загального бтрубту), у порiвняннi з контрольною групою вiдповiдно 22,83% (td = 0,98, P < 0,05), 7,12% (td = 1,92, P < 0,05) and by 20,54% (td = 0,86, P < 0,05). БЫьшкть гепатоцитiв мали велит ядра та ттенсивне забарвлення цитоплазми. Це вказуе на вiдсутнiсть альтеративних процеав в органiзмi. Резуль-тати до^джень дозволяють зробити висновок, що включення 0,2% альфасорбу до складу корму, контамтованого мжотоксинами, значно зменшуе гх негативний вплив на оргашзм поросят, позитивно впливае на прирости, перебiг метаболiч-них процеЫв, також сприяе, зниженню у печтщ середтх розмiрiв ядер гепатоцитiв, стимулюе бiосинтетичш процеси у цих клтинах та забезпечуе формування повног структури гiстогематологiчних бар 'ерiву органiзмi.
K.mnoei слова: альфасорб, показники кровi, жива вага, печiнка, мжотоксини, поросята.
Introduction
Various toxic substances and mycotoxins can come to the organism with food. It must be remember when organizing a high-grade feeding of animals.
Mycotoxins are secondary metabolits. They are formed in process of life of moldy fungus (Trufanov, 2011; Brezvyn et al., 2018). Most mycotoxins are classified as first-class of toxicity compounds. They show the dermatonecrotic, hepatotoxic, carcinogenic, mutagenic, teratogenic and immunosuppressive action in animals organism (Dukhnytskyi et al., 2011; Kotsyumbas et al., 2016).
Mycotoxins cause severe diseases - mycotoxicoses -in organisms of human and animals (Ivanytskyi, 2004). They are difficult to diagnose, cause great economic damage. The economic loss is the reduce productivity and death of animals, as a result of feeding unsuitable products and feeds (Tremasov, 2005).
Mycotoxicoses causes the changes of metabolism. It is the main problem of mycotoxicoses. The signs of their action are manifested when it changes go beyond compensatory mechanisms. The animals stay healthy if compensatory mechanisms work effectively but functioning of that mechanisms requires additional energy and nutrients consumption. This causes an increased feed consumption for animals' productivity. There fore mycotoxicoses can't be treated by tradition medicines. It is necessary to correct metabolic disorders caused by toxins. But achievements in this direction are limited. Prevention of mycotoxicoses by using adsorbents is most common. They are added to feed for binding mycotoxins and limiting their intake into the body (Krjukov, 2011).
Sorbents are able to effectively bind and remove from the body through the digestive tract with a therapeutic or prophylactic purpose various endogenous and ekzogenous substances, microorganisms and they toxins, supermolec-ular structures and cells (Gusejnov, 2012). So the method of sorbtion is considered the most effective and safe for animals (Kondrahin, 1987; Antipov et al., 2007).
Awade range of feed additives of sorbents of foreign and domestic production is presented today on the market of veterinary drugs in Ukraine. They are recommended to be used for neutralization of fodder from mycotoxins and to increase the productivity of animals. They can be di-
vided into three groups: inorganic, organic and combined (Kotsiumbas et al., 2010).
Alfasorb is one of the detoxicant feeds which has organic origin. It produces by LLC SPE "Ariadna" (Odessa). Alfasorb was obtained on the basis of isolation of cellulose biopolimers with a number of active centers from plant fodder. Such raw materials are dietary fibers of brain which subjected to multilevel processing. Due to this transformation of polymeric carbohydrate chains occurs and many active binding sites are formed with mycotoxins.
Efficiency of use Alfasorb is confirmed when it is infected in the diet of repairing pigs in the amount of 0.02% by the weight of the compound feed by previous studies. It positively influenced the digestibility of nutrients and productive qualities. The use of Alfasorb to detoxity fodder when raising young pigs was not performed (Kajsin et al., 2012).
Therefore the aim of the work is to study the effectiveness of the use of Alfasorb for the neutralization of mixed fodders affected by mycotoxins and they impact on blood's indexes and productive qualities of piglets of Large White Breed.
To achieve the goal next tasks were set: to carry out the analysis of live weight of pigs after application of Alfasorb; to study morphological and biochemical parameters of blood after application of Alfasorb; to conduct a histological examination of the liver.
Matherial and methods
Experience was conducted in conditions of the farm of Shiryaevsky district of Odessa region. 30 pigs of Large White Breed were used in the experiment. They were divided into two groups according to the principle of analogs, taking into account the live weight, age and previous energy of growth. During the experiment the animals of the control and experimental groups were kept under the same conditions. The experience lasted 60 days.
Animals of the control group received mixed feed (basic diet) that was produced in the conditions of the farm (corn, barley, wheat, soybean meal, fish flour, pre-mix, salt). The pigs of experimental group in addition to the basic diet received Alfasorb (LLC SPE "Ariadna", Odessa, Ukraine) in quatities of 1 kg per ton of feed.
The presence of mycotoxins in feads was determined on an immune-enzyme analyzer StatFax 2100 using testsystem Ridascreen (Germany).
Dynamics of changes in live weight and the growth rate of piglets were studied on the basis of monthly individual weighting data and the calculation of the average daily growth.
We determined hematologic (number of erythrocytes and leukocytes) and biochemic (the content of hemoglobin, total protein, urea, creatinine, activity of ala-ninaminotransferaze, aspartataminotransferaze and lac-tatdehydrogenaze, total calcium and inorganic phosphorus) blood's indexes for study the effect of feed additive Alfasorb on the physiological state of animals at 4 months of age (Kondrahin, 1987). Blood samples for studies were taken in the morning before feeding from the ear vein.
The slaughter was carried out for three animals from the control and experimental groups in order to determine the degree of the negative effect of mycotoxins on the organism of piglets and the effectiveness of decontamination of feeds by Alfasorb. A morphological and histologi-cal study of some internal organs was carried out (Merkulov, 1969).
Statistical processing of data was carried out on a personal computer. We used computer program "Microsoft
Table 1
Live weight of experienced piglets (M ± m, n = 15)
Excel". The criteria for the reliability of differences between groups were determined by the Student's table (Lakin, 1980).
Results and discussion
Animals of control and experimental groups on live weight practically didn't differ at the beginning of the experiment (Tab. 1). The introduction of Alfasorb in the compound feed contributed to on increase in the live weight of the piglets of the experimental group. The live weight has increased in comparison with the animals of the experimental group at the and of the first month of drowing on 0.95 kg (or on 3.24%) and at the end of the second month on 2.26 kg (or on 5,32%). While the pigs of the experimental group significantly exceeded the animals of control group by live weight at the end of the second month of growing (td = 3.79, P < 0.01).
The analysis of the average daily weight gain of piglets showed that it was higher in period of growth in the animals of experimental group. It composed 456.33 g. It's 36.83 g more (or on 8,76%) than in animals of control group (td = 7.37, P < 0.001).
Group
At the beginning of the experiment, kg
At the end of the first months, kg
At the end of the second months, kg
Average daily growth for the period, g
Control Experimental
17.30 ± 0.23 17.35 ± 0.36
29.30 ± 0.31 30.25 ± 0.37
42.50 ± 0.39 44.76 ±0.45*
420.00 ± 3.47 456.83 ± 3.74***
Remark: * - P < 0.05; *** - P < 0.001 in comparison with the control group
Blood is one of the most important tissues of the body. It plays an important role in vital activity and fully reveals the picture of metabolic processes. It closely contacts all cells and organs due to the widely branched network of vessels and capillaries. Thus the blood provides the possibility of trofic and respiratory
A study of the blood composition of piglets at the end of the experiment showed that the blood indexes of ani-
mals of the control and experimental group were within the physiological norm (Kondrahin, 1987) (Tab. 2).
Alfasorb in the diet significantly reduced the negative effect of mycotoxins on the body of piglets. It contributed to some improvement in their morphological and biochemical blood indexes.
Table 2
Blood's indexes of piglets of experimental groups ( M ± m, n = 5)
Indexes Control vjiuup Experimental
Erythrocytes, million/liter 7.17 ± 0.17 7.47 ± 0.29
Leukocytes, thousand/liter 5.27 ±0.69 14.25 ± 1.45
Hemoglobin, g/l 95.27 ±2.15 102.93 ± 1.97*
Total protein, g/l 67.30 ±2.56 75.85 ± 2.39*
Urea, mmol/l 5.81 ±0.71 4.73 ± 0.85
Creatinine, mcmol/l 76.43 ± 1.45 72.35 ± 1.58
Common bilirubin, micromole/l 2.23 ± 0.27 1.85 ± 0.35
Alaninaminotransferase, U/l 55.73 ±2.58 45.3 ± 3.51
Aspartataminotransferase, U/l 67.45 ±2.86 57.53 ± 3.86
Laktatdehydrogenase, U/l 633.80 ± 13.86 697.55 ± 23.35
Total calcium, mmol/l 2.57 ±0.37 2.70 ± 0.25
Inorganic phosphorus, mmol/l 1.87 ±0.23 2.35 ± 0.17
Remark: * - P < 0.05 in comparison with the control group
In the animals of the experimental groups there was an increase in the number of red blood cells by 4.18%, hemoglobin by 7.52% and total protein by 9.73% in comparison with the control. The piglets of the experimental group respectively td = 2.62 and td = 2.44 (P < 0.05) for protein metabolism parameters (hemoglobin, total protein ).
The content of total protein in the serum of pigs is an important diagnostic parameter. It is associated with changes in metabolism in the body. The increased content of total pritein in the experimental group in comparison with the control indicates an increase in oxidation-reduction and plastic processes in the organism of animals in connection with the use of Alfasorb. Decrease in the total protein content in the blood serum of piglets from the control group is due to the fact that the mycotoxins were introduced into the body of the pigs. They caused inhibition of protein synthesis in the liver.
An unreliable decrease in blood serum levels which characterize the level of intermediate metabolism of proteins in the body (content of urea, creatinine and common bilirubin) was established in the animals of the experimental group as compared to the control group respectively by 22.83% (td = 0.98, P < 0.05), 7.12% (td = 1.92, P < 0.05) and by 20.54% (td = 0.86, P < 0.05).
A decrease in the activity of transamination enzyms revealed in animals from experimental group by 16,43% (td = 1.93, P > 0.05 ) and by 17.24% (td = 2.26, P < 0.05). This indicates a favorable effect of Alfasorb on the functional activity of the liver. An increase in the activity of these enzymes is observed in hepatitis, muscular dystrophies, injuries of animals. But these enzymes are not strictly specific.
The activity of lactatdehydrogenase in pigs from experimental group increase by 10.05% (td = 2.36, P > 0.05) relative to control. It indicates the intensification of glycolitic way of catabolism of glucose. This is indirect evidence of activation of bioenergetics processes in organism of pigs with the use of Alfasorb.
The study of mineral metabolism showed that the content of total calcium in blood serum in the animals of the control group was on 5.06% (td = 0.52, P > 0.05) higher in comparison with the animals in the control group. The animals of the control group were inferior to the pigs of the experimental group by 25.67% (td = 1.68, P > 0.05) by the content in the serum of inorganic phosphorus at the same time.
Histological examination is the only and decisive method of diagnosis in many cases regardless of the etiology (Ivanytskyi, 2004). A well-defined lobular girdle structure of the liver was established in experimental group of piglets. Most hepatocytes had large nucleus and intense color of the cytoplasm. Cells of the mononuclear phagocyte system showed less activity. This indicates the absence of alterative processes in the body.
The signs of hemodynamic disorders were less pronounced in the vessels of the microcirculatory organ in experienced piglets with a sharp decrease in the permeability of the vascular wall. This was evidenced by low parasinusoidal spaces and practically complete absence of perivascular edema along the portal tract.
Congestive hyperemia, discomplexation of beams and
hepatocytes, granular and fatty dystrophy, necrosis of some hepatocytes were observed in the liver of piglets of the control group. The liver was characterized by a large variety of volumes of hepatocyte nuclei, the presence of a large number of light cells that had a reduced level of glycogen content and small foci of macrophage proliferation.
Conclusions
The results of the studies make it possible to assert that the inclusion the 0.2% Alfasorb to the compound of feed which contaminated with mycotoxins significantly reduces their negative effect on the piglets organism, has a positive effect on the growth rate, on the course of metabolic processes, also it promotes an increase in the liver of medium-sized hepatocyte nuclei, stimulates biosynthet-ic processes in that cells and ensures the formation of a full structure of histohematological barriers in the organism.
Prospects for further research. In the future it is planned to conduct a study of the effect of Alfasorb on the organism of chicken.
References
Antipov, V.A., Semenenko, M.P., & Matjushevskij, A.S. (2007). Perspektivy primenenija prirodnyh aljumosili-katnyh mineralov v veterinarii. Veterinarija, 8, 54-58 (in Russian).
Brezvyn, O.M., Rudyk, G.V., & Guta, Z.A. (2018). Influence of HammecoTox and Zeolitis on morphological and biochemical indicators of rat's blood under conditions of experimental fumonisin toxicosis. Ukrainian Journal of Veterinary and Agricultural Sciences, 1(1), 23-29. doi: 10.32718/ujvas1-1.04. Dukhnytskyi, V.B., Khmelnytskyi, H.O., Boiko, H.V., Ivashchenko, V.D. (2011). Veterynarna mikotoksykolohiia: navch. posibn. K.: Ahrarnaosvita. https://nubip.edu.ua/node/19867 (in Ukrainian). Gusejnov, M.M. (2012). Jenterosorbcija pri ostryh kishechnyh infekcijah molodnjaka krupnogo rogatogo skota. Veterinarnaja medicina, 3(4), 70-71. http://www.veterinarymedicine.ru/num3-4-2012.html#29 (in Russian).
Ivanytskyi, M.Ye. (2004). Histolohichna kharakterystyka mikotoksykoziv svynei. Visnyk ahrarnoi nauky. 8, 33-35 (in Ukrainian). Kajsin, L.G., Bivol, L.V., & Kovalenko, A.V. (2012). Vlijanie adsorbenta "Prajmiks-Al'fasorb" na pere-varimost pitatelnyh veshhestv i produktivnye kachest-va remontnyh svinok. Zhivotnovodstvo i veterinarnaja medicina, 3(6), 33-38. http://moyuniver.net/vliyanie-adsorbenta-prajmiks-alfasorb-na-perevarimost-pitatelnyx-veshhestv-i-produktivnye-kachestva-remontnyx-svinok/ (in Russian). Kondrahin, I.P. (1987). Klinicheskaja laboratornaja diag-nostika v veterinarii: sprav. Izdanie. M.: Agropromiz-dat (in Russian). Korzunenko, O.F., Obrazhei, A.F., & Vasianovych, O.M. (2005). Mikromitsety kormiv u period zberihannia ta
HayKOBHH BicHHK ^HyBME iMeHi C.3. IW^KOTO, 2018, T 20, № 88
yikhnii toksyhennyi potentsial. Veterynarna bio-tekhnolohiia, 6, 68-73 (in Ukrainian).
Kotsiumbas, I.Ia., Avdosieva, I.K., & Brezvyn, O.M. (2010). Efektyvnist vaktsynatsii proty virusnykh zakhvoriuvan ptytsi u razi zastosuvannia detok-sykantiv mikotoksyniv. Naukovyi visnyk veterynarnoi medytsyny: zb. nauk. prats. Bila Tserkva, 6(79), 6369 (in Ukrainian).
Kotsyumbas, l., Brezvyn, O., Rudyk, G., Guta, Z., & Gutyj, B. (2016). Effect of fumonisin toxicosis on rats. Pasze przemyslowe. Lublin, 3/4, 108-115. http://www.paszeprzemyslowe.pl/index.php/33-artykuly-2016a/3-4-2016/424-effect-of-fumonisin-toxicosis-on-rats.
Krjukov, V.S. (2011). Opasnost mikotoksinov v mo-lochnom skotovodstve. RacVetlnform, 12(124), 3343 (in Russian).
Lakin, G.F. (1980). Biometrija. M.: Vysshaja shkola (in Russian).
Merkulov, G.A. (1969). Kurs patologogistologicheskoj tehniki. M., Leningradskoe otdelenie (in Russian).
Tremasov, M.Ja. (2005). Profilaktika mikotoksikozov zhivotnyh v Rossii. Veterinarija sel'skohozjajstvennyh zhivotnyh, 1, 45-51 (in Russian).
Trufanov, O.V. (2011). Monitoring zagrjaznennosti mikotoksinami zerna i kormov v Ukraine v 2005-2010 gg. Suchasni problemy toksykolohii, 1(2), 35-39. http://www.medved.kiev.ua/web_journals/arhiv/toxicol ogy/2011/1-2_2011/str35.pdf (in Russian).
