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Abstracts. PHYTOPHARM 2017
5. Several African plants are used topically to treat wounds and angiogenesis is an important sub-phase of the proliferative stage of wound healing. Using a zebrafish model we confirmed pro-angiogenic properties of several plant species that promoted the growth of new capillaries from pre-existing blood vessels.
6. There has been renewed interest in the discovery of antiepileptic compounds of natural origin. Using the zebrafish model, several "mind-and-mood" plants used in African traditional medicine were screened to confirm their use in treating epilepsy.
7. Traditional healers rarely rely on a single species to treat disorders but most often administer polyherbal formulations to patients. The value of synergy studies to enhance pharmacological efficacy will be demonstrated.
The premise for studying the plants mentioned above is all based on very rich (yet fragile) indigenous knowledge systems. An urgent need exists to explore the unique South African flora with the ultimate goal of improving the quality of life of mankind and to stimulate the bio-economy of the region.
THE EFFECT OF DIFFERENT SOLVENTS ON PHENOLIC AND FLAVONOID CONTENTS AND FREE RADICALS SCAVENGING ACTIVITY OF MACLURA COCHINCHINENSIS STEM EXTRACTS
© Boonyadist Vongsak1, Nutsuda Inson1, Savita Chewchinda2
1 Faculty of Pharmaceutical Sciences, Burapha University, Saensook, Muang, Chonburi, Thailand;
2 Department of Food Chemistry, Faculty of Pharmacy, Rajathevi, Mahidol University, Bangkok, Thailand
Maclura cochinchinensis Corner have been used as traditional herbal medicine throughout tropical and subtropical countries. The stem of this plant has traditionally been used to treat, skin infection, fever and jaundice. Based on recent study, M. cochinchinensis has in vitro prominent value of antioxidant and free scavenging activity. The bioactive compound in this plant was found to be morin, a class of flavonols. To obtain the maximum yields of the compound, which consequently influence the free radicals scavenging activity, varying extraction solvents; distilled water, 70 percent ethanol, and ethyl acetate, were examined by soxhlet extraction method. The contents of total phenolics and total flavonoids, free radical scavenging activity using DPPH and ABTS free radical scavenging methods and ferric reducing antioxidant power (FRAP) assay of each extract were quantitatively determined. Seventy percent ethanol extract gave the highest yields of crude extract (25.23% dry weight), while ethyl acetate extract
gave the lowest yield (11.33% dry weight). The 70% ethanol extract exhibited significantly different amount of total phenolic contents (4.88 ± 0.25 gram Gallic acid equivalent/100 gram dry powder) and total flavonoid contents (2.80 ± 0.22 gram Morin equivalent/100 gram dry powder). For free radicals scavenging activity, the extracts from these various solvents revealed slightly different activity (IC50 16.39 - 18.87 ig/mL, IC50 7.05 -9.52 ig/mL and 0.82- 1.08 mmol Fe2+eqivatent/100 gram extract for DPPH, ABTS and FRAP assays, respectively), while ascorbic acid, a positive control, displayed the activity with IC50 5.10 ig/mL, 5.42 ig/mL and 5.56 mmol Fe2+eqivatent/15000 gram extract for DPPH, ABTS and FRAP assays, respectively. Thus, the results point that M. cochinchinensis stem extracts illustrated strong free radicals scavenging activity and 70 percent ethanol extract should be recommended as the extraction solvent because of maximum contents of phenolics and flavonoids for pharmaceutical development.
ISOLATION OF THREE NEW FLAVONOID C-GLYCOSIDES FROM IRIS LACTEA
© Whaley A.K., Luzhanin V.G.
St. Petersburg State Chemical-Pharmaceutical Academy, St. Petersburg, Russia
Iris lactea Pall. is a rhizomatous perennial flowering plant distributed throughout mostly temperate and some tropical areas of Asia. Early research of plants from the Iris genus mainly focused on the rhizomes, of which flavonoids were reported to be the major secondary metabolites. In previous phytochemical publications flavone C-glycosides
have been found in the aerial parts of Iris lactea. Previous study on Iris lactea suggested the presence of flavone C-glycosides derivatives of embinin [1].
Up to now, three rhamnose-residue acetate ester derivatives of embinin, 5-hydroxyl-7,4'-dimethoxyflavone-6-C-[O-(a-L-2-acetylrhamnopyranosyl)-1^2-p -D-
Obzory po kliniceskoj farmacologii i lekarstvennoj terapii [Reviews of clinical pharmacology and drug therapy] vol. 15/2017/suppLement 1
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