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Abstracts. PHYTOPHARM 2017
PHENOLIC COMPOUNDS AND ANTIOXIDANT POTENTIAL OF CRATAEGUS MONOGYNA FRUITS FROM ROMANIA
© Erzsebet Varga1, Bernadett Papp2, Mircea Dumitru Croitoru3, Erzsebet Majai Fogarasi3, Fülöp Ibolya3, Domokos Erzsebet4, Laczko Zöld Eszter1
1 University of Medicine and Pharmacy of Ttrgu Mures, Department of Pharmacognosy and Phytotherapy;
2 Faculty of Pharmacy, Student;
3 Department of Toxicology and Biopharmacy;
4 Department of Botany Ttrgu Mure
Crataegus monogyyna L., known as common hawthorn is a native species in Europe, also widespread in Romania. The fruits are used in phytotherapy. Methanol, methanol-water (1:1) and water were used to prepare extracts from frozen fruit. Spectrophotometric methods were used to determine the phenol, flavonoid and anthocyanin content of extracts [1, 2]. The content of total phenol compounds varies from 745 mg gallic acid in water extracts to 1135 mg gallic acid in methanol:water (1:1) extracts of 100 g frozen fruit. The content of total flavonoids is between 175 mg in methanol extracts and 735 mg in water extracts of 100 g frozen fruits. Anthocyanins content were 1.50 mg cyanidin 3 glucoside/g of frozen fruits sample. The differences in total phenol compounds content depend on used extraction medium. The antioxidant activity was evaluated by ABTS and photochemiluminescens (PCL, Photochem®, Analytic Jena) methods. The antioxidant activity of the methanol-water extract is higher with the PCL test and methanol extract antioxidant activity is higher with the ABTS test (methanol-water extract
IC50 = 0.89 mg/ml for ABTS; 1324 imol AAE/g fruit for PCL; methanol extract IC50 = 0.54 mg/ml for ABTS; 458 imol AAE/g fruit for PCL). Chlorogenic acid and hyperoside, isoquercitrin flavonoids were identified by RP-HPLC-UV-VIS from methanol extracts. The present study represents a contribution to the chemical characterization of phenolic compounds from wild fruits with antioxidant activity and traditionally used in phytotherapy.
Acknowledgements. We thank the Hungarian Academy of Sciences and Studium Prospero Foundation (0350/26.02.2016) for the financial support.
References:
1. Balogh E. 2010. Antioxidans kapacitas meghataro-zasa es ennek kialakitasaban szerepet jatszo veg-yületek vizsgalata bogyos gyümölcsök eseteben, PhD thesis, Budapest, Corvinus Univ
2. Banderas-Tarabay JA, et al. 2015. J. Pharmacoge-nomics Pharmacoproteomics. 6:4.
WHERE ANCIENT KNOWLEDGE AND MODERN SCIENCE MEET -EXAMPLES FROM SOUTH AFRICAN FLORA
© Alvaro Viljoen, Fikile Mhlongo, Talent Chipiti, Baatile Komane Mofokeng
Department of Pharmaceutical Sciences, Tshwane University of Technology, Pretoria, South Africa
South Africa is home to several UNESCO world heritage sites, including the Sterkfontein caves referred to as the Cradle of Humankind. This site has produced a large number, as well as some of the oldest, hominid fossils ever found, some dating back as far as 3.5 million years. South Africa is often referred to as the 'rainbow nation', a phrase which epitomises the country's cultural diversity. It is this unique blend of ancient civilisation and cultural diversity all situated in a botanical wonderland which provides the most ideal opportunities for pharmacognosy research in South Africa. Using various directives from ancient knowledge and current traditional plant use, we have explored the phytochemistry and biological properties of South Africa's indigenous flora and several examples will be discussed:
1. Sceletium tortuosum has emerged as a nutraceutical to treat CNS-related orders. The importance
of respecting traditional practices will be highlighted and the tedious processes involved in developing new commercial products will be summarised.
2. Helichrysum species are used extensively in traditional rituals and produce a variety of bioactive molecules. South Africa harbours more than 250 Helichrysum species which remain largely unexplored.
3. Bulbine frutescens has similar applications to Aloe vera which include; to promote and maintain healthy skin. The value of in vivo studies to confirm traditional uses will be highlighted.
4. African nut oils are traditionally used by southern African women as moisturising agents on the face, feet and hands. Results from a clinical study have confirmed the moisturising, hydrating as well as occlusivity properties of Marula and Baobab oils applied topically.
Obzory po kliniceskoj farmacologii i lekarstvennoj terapii [Reviews of clinical pharmacology and drug therapy]
vol. 15/2017/suppLement 1
