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Biosystems
Diversity
ISSN 2519-8513 (Print) ISSN 2520-2529 (Online) Biosyst. Divers., 26(2), 132-138 doi: 10.15421/011821
Interspecific differences of antioxidant ability of introduced Chaenomeles species with respect to adaptation to the steppe zone conditions
N. Khromykh, Y. Lykholat, L. Shupranova, A. Kabar, O. Didur, T. Lykholat, Y. Kulbachko
Khromykh, N., Lykholat, Y., Shupranova, L, Kabar, A., Didur, O., Lykholat, T., & Kulbachko, Y. (2018). Interspecific differences of antioxidant ability of introduced Chaenomeles species with respect to adaptation to the steppe zone conditions. Biosystems Diversity, 26(2), 132-138. doi:10.15421/011821
Plants of the genus Chaenomeles are traditionally used in the countries of South-East Asia, due to their high nutritional and health-promoting properties. However, the successful introduction of species promising for gardening from geographically remote areas requires the study of plant ontogeny under the conditions of new habitat. This is a very substantial problem for the steppe zone, where the continental climate has features of aridity and complicates the process of increasing the diversity of fruit crops by introducing the desired species. The present study aims to assess the effectiveness of the protective enzymatic system of different Chaenomeles genotypes subject to a steppe climate as well as the accumulation of the biologically active compounds with high antioxidant capacity. The study was performed on the basis of the introduced horticultural plants collection in the Botanical Garden of the DNNU, and the Chaenomeles fruits, leaves, and the seeds were examined. The highest activity of catalase, benzidine-peroxidase and guaiacol-peroxidase, and the greatest enzymes activation during vegetation were found in leaves of Ch. cathayensis and Ch speciosa, while the lowest activity was in leaves of both Japanese species. The biggest total phenolic content in the isopropanolic plant extracts, determined by Folin-Ciocalteau assay, was found in leaves of Ch. x superba, Ch. x californica and Ch. cathayensis (44.8, 52.8, and 43.6 mg GAE/g WW); a less high level was found in leaves of Ch japonica and Ch. japonica var. maulei(43.1 and 40.2 mg GAE/g), while the lowest was in leaves of Ch. speciosa (29.3 mg GAE/g). The total flavonoids content determined using the aluminum chloride method, did not differ by variety or species in the plant leaves, being in the range of 2.6-2.9 mg of RE per g WW (accordingly, in leaves of Ch. japonica var. maulei and Ch x californica). The high total reducing power determined by potassium ferricyanide assay was found in leaves of both hybridogenic species and Ch. cathayensis (respectively, 11.6, 14.1, and 11.4 AAE/g WW); leaves of both Japanese species had slightly lower values and the lowest was in leaves of Ch speciosa (7.7 AAE/g). In the Chaenomeles fruits, the total phenolic content was the lowest in Ch. speciosa (17.8 mg GAE/g), average in both Japanese species (28.7 and 27.8 mg GAE/g), and the highest (33.3 mg GAE/g) was in Ch. cathayensis. The flavonoid accumulation was highest in the fruits of Ch. cathayensis and Ch. japonica var. maulei (0.67 and 0.63 mg RE/g), intermediate in both hybridogenic species and Ch japonica (accordingly, 0.57, 0.42 and 0.38 mg RE/g), and the lowest in Ch. speciosa (0.30 mg RE/g). The total reducing power of Chaenomeles fruit was lower as compared to leaves, and decreased from 11.2 to 5.7 mg AAE/g in the series Ch cathayensis > Ch. x californica > Ch. japonica > Ch. japonica var. maulei > Ch x superba > Ch. speciosa. High correlation coefficients between total reducing power and total phenols content in the Chaenomeles leaves and fruits (respectively, r = 0.96 and r = 0.95, P < 0.05) confirm the significant contribution of phenolic compounds to the antioxidant capacity. The study results indicate a high antioxidant capacity of the Chaenomeles species in the conditions of the steppe climate due to the antioxidant enzymes activity and the accumulation of a significant amount of phenolic metabolites in leaves and fruits.
Keywords: Chaenomeles; fruits; leaves; antioxidant enzymes; phenols; flavonoids; total reducing power
Oles Honchar Dnipro National University, Dnipro, Ukraine
Article info
Received 19.04.2018 Received in revised form
07.05.2018 Accepted 10.05.2018
Oles Honchar Dnipro National University, Gagarin ave., 72, Dnipro, 49010, Ukraine. Tel.: +38-050-487-87-17 e-mail:
khromykhN@ukr. net
Introduction
Horticultural plants are an inexhaustible resource for human food (Ros-García et al., 2004), but their role as a source of the biologically active compounds for health is not less important (Beecher, 2003; Vrhovsek et al., 2004; Górnas et al., 2014). The antioxidant properties of plant second metabolites, which have a different chemical structure, contribute to the neutralization of oxidative stress, thereby facilitating or eliminating the diseases caused by it (Costa et al., 2009; Sahan et al., 2012). Many of these compounds are able to provide preventive and therapeutic effects, supplementing or even replacing traditional medicines, and can also serve as plant raw materials for the production of medicaments (Cheynier, 2005; Mojzer et al., 2016).
The introduction of alien fruit plants into cultivation in different regions contributes to the expansion of the local spectrum of species possessing health promoting properties for people. However, it is necessary to take into account the fact that the accumulation of biologi-
cally active compounds in plants depends to a large extent on the region of origin of species and varieties, and the new environmental conditions (Alexeyeva et al., 2016), including the effect of climatic factors (McGhie et al., 2005; Hafez-Taghva et al., 2016). This is particularly relevant for the Steppe Dnieper of Ukraine, where the climate has continental features and is not equally favourable for all introduced plant species, even within the same genus. Therefore, an assessment of the stability and metabolic activity of introduced fruit plants is necessary, since it enables the optimal selection of the most promising species for industrial cultivation. Adaptation of desired fruit plants to unfavourable environmental conditions can be improved with the help of modern methods, such as the treatment with the synthetic plant growth regulators (Shcherbyna et al., 2017).
In the steppe zone of Ukraine, the introduction of horticultural plants from remote geographical areas has been carried out in the Botanical Garden of Dnipro National University for many years, including several natural and hybridogenic species of the genus Chaenomeles. The adap-
tation of these introduced plants to the new habitat was assessed from a phenological position, but the plants' capacity for biosynthesis and the accumulation of secondary metabolites has not been investigated. Meanwhile, the fruits and other plant organs of the Chaenomeles species are a rich source of polyphenols (Mao et al., 2016), organic acids, alcohols, ketones, aldehydes, vitamin C (Watychowicz et al., 2017), tri-terpenes and procyanidins (Du et al., 2016), as well as the macro- and microelements (Baranowska-Bosiacka et al., 2017). It is natural that the fruits of various species of the Chaenomeles genus have been used for centuries in China and other countries of Southeast Asia for the treatment of cardiovascular diseases, anemia, rheumatism and gout (Du et al., 2013; Watychowicz et al., 2017).
Nowadays, the numerous studies confirm the high prophylactic and therapeutic effects of preparations obtained from the fruits, leaves and twigs of various Chaenomeles plants, including their anti-inflammatory (Strugala et al., 2016; Han et al., 2016; Suh et al., 2017), anti-influenza (Zhang et al., 2014), and hepatoprotective activity (Ma et al., 2016; Baranowska-Bosiacka et al., 2017; Zaklos-Szyda & Pawlik, 2018), as well as their effectiveness in the prevention and treatment of diabetes (Watychowicz et al., 2017). In addition, the significant anti-carcinogenic properties of the extracts from Ch. japonica fruits, particularly against breast cancer (Lewandowska et al., 2013) and colon cancer (Gorlach et al., 2011; Owczarek et al., 2017) has been established. Recent studies have revealed immunoregulatory and antiparkinsonian properties of the extracts from Ch. speciosa fruits (Zhang et al., 2014). The vast majority of study results indicate that biological activity of the preparations are to the greatest extent associated with the presence of the polyphenols (Ha-mauzu et al., 2006; Brglez et al., 2016; Zaklos-Szyda & Pawlik, 2018). The aim of the present work was to assess the adaptive capabilities of different Chaenomeles genotypes to the arid climate of the Steppe Dnieper, taking into account the activity of antioxidant enzymes and the content of secondary metabolites in leaves and fruits of plants.
Materials and methods
Study area. The research was performed in 2016-2017 in the Botanical Garden of Oles Honchar Dnipro National University (48°26'14'' N, 35°02'35'' E). The original soil cover is usual low-humus medium loamy chernozems; the humus content in the plow layer is 4-5%. The regional climate has distinct continental features, such as seasonal droughts with high temperatures, unstable moisture and dry hot winds. During the vegetation period of the Chaenomeles plants, several periods with low relative humidity (in the range 31-52%) were noted, in particular in April and June, and especially long in July and August. Drought periods were accompanied by a high air temperature, which reached 25-30 °C in the second decade of June, 32 °C in July, and 35 °C in August.
Data collection. The study objects were the leaves, ripe fruits, and seeds of some natural and hybridogenous plant species of the genus Chaenomeles Lindley (family of Rosaceae, subfamily Maloideae).
Table 1
Phenological differences of genus Chaenomeles species in 2017
Systematic and taxonomic characterization of the species was made in accordance with Rumpunen (2002) and Watychowicz et al. (2017).
Ch. japonica (Thunb.) Lindl. (Japanese quince) is common in central and south Japan at an altitude 100-2,100 m on hillsides, and on riverbanks and lakeshores. It is a dwarf shrub 0.6-1.2 m in height with ovoid leaves. The flowers are small, most often the colour of a salmon or an orange. Small fruits are similar in shape to apples that mature early and have an extraordinary fragrance. This is the most enduring species in the genus Chaenomeles.
Ch. japonica var. maulei was introduced from Japan in 1869 by Maule, the owners of nurseries at Bristol, England. This plant is taller, has single flowers, painted in colors from pink to orange.
Ch. speciosa (Sweet) Nakai (flowering quince) is found in China in mountainous terrain at different altitudes. The species is winter hardy and drought-resistant. It is a deciduous or semi-evergreen shrub 2-3 m in height. The leaves are ovate with jagged edges, the flowers are often red, but can be white and pink. The fruits are very diverse in shape, size, and time of ripening.
Ch. cathayensis (Hemsl.) Schneider (Chinese quince) is found in the wild in China and southern Tibet at an altitude of over 3,000 m, but its winter hardiness is average, as well as drought resistance. Usually it is a shrub up to 3 m high, but can be formed as a tree. The leaves of this plant are elliptical with notches, flowers are white or pink. The fertility is plentiful, but the fruits ripen late; the shape of the fruit is ovoid, the length reaches 15-20 cm.
Ch. x superba (Frahm) Rehder (Ch. japonica x Ch. speciosa, Su-perba group) is a shrub 2 m in height, the leaves are similar to Ch. japonica leaves. Flowers are of medium size and may be white, pink, orange or red. Fruits are more often in the form of apples, larger than in Ch. japonica and they mature later.
Ch. x californica Clarke ex Weber [Ch. cathayensis x (Ch. x superba), Californica group] is a shrub more than 2 m in height with lanceolate leaves. Flowers are large, usually pink or red, or mixed colours. It produces a variety of fruits ranging from medium to large, the shape is ovoid or apple. This hybrid has an average frost resistance and drought resistance.
All plants of Chaenomeles genus are alien species in the Steppe Dnieper, and were introduced in the region several decades ago. Plant leaves were selected during the period of flowering, and the fruits were selected as they mature, according to phenological phases (Table 1).
Data analysis. Determination of the antioxidant enzymes activity in plant leaves was performed by spectrophotometric method in the supernatants after centrifugation of the crude extracts (100 mg of plant fresh mass homogenized in Tris-HCl buffer, pH 7.0). Catalase activity (CAT) was detected at 410 nm by ammonium molibdate method in accordance with Góth (1991), and calculated using a calibration graph prepared on H2O2 solutions. Activity of benzidine-peroxidase (BPO) was measured at 490 nm by the method of Gregory (1966), and activity of guaiacol-peroxidase (GPO) was detected at 470 nm according to the method of Ranieri et al. (1997).
Introduced species
Introduction time
Source of the introduction
Phenological phases of plant growth
leaves appearance florescence ripe fruit appearance
Ch. speciosa 1996 Moldova, Kishinev 18.03 25.03- 19.04 27.09-05.10
Ch. cathayensis 1996 Moldova, Kishinev 21.03 29.03- 13.04 10.10.-25.10
Ch. japonica 1996 Moldova, Kishinev 20.03 27.03- 10.04 21.09-28.09
Ch. japonica var. maulei 1955 Tajikistan, Khorog 19.03 26.03- 13.04 14.09-01.10
Ch. x superba 1996 Moldova, Kishinev 19.03 29.03- 14.04 25.09-10.10
Ch. x californica 2006 Crimea, Yalta, State Nykytsky botanical garden 20.03 28.03- 12.04 25.09-10.10
Extracts containing the phenolic compounds were obtained by holding the raw crushed plant material (200 mg of fruit flesh, and 500 mg of leaves) in 5 ml of 80% isopropanol for 24 hours at room temperature. After this, the crude extracts were filtered and stored at 4 °C until analysis. The Folin - Ciocalteau method (Singleton et al., 1999) in modification (Nwanna et al., 2013) was used for the total phenolic content measurement. In brief, 0.2 ml of plant extract diluted with 0.2 ml of distilled water was oxidized with 1 ml of 10% Folin -
Ciocalteau reagent, and neutralized by 7.5% Sodium carbonate solution. The reaction mixture was incubated for 40 minutes at 45 °C and cooled. Optical density of the samples was measured at a wavelength of726 nm. The total phenolic content was calculated using a calibration graph prepared on the solutions of Gallic acid (GA), and expressed as mg GA equivalents per 100 g of wet weight (mg GAE/100 g WW).
The aluminum chloride spectrophotometric method (P^kal & Pyr-zynska, 2014) was used for the measurement of total flavonoids content
in the Chaenomeles plants. Briefly, 1 ml of aluminum chloride (AlCl3) solution, and 1 ml of sodium acetate were added to 2 ml of isopropanol extract. The reaction mixture was maintained for 10 minutes at room temperature, and the optical density measured at 425 nm. The content of flavonoids was calculated using a calibration graph prepared on rutin solutions, and the result was expressed as mg rutin equivalents per 100 g of plant wet weight (mg RE/100 g WW).
The total reducing power (TRP) of the Chaenomeles plants was determined in accordance with Pulido et al. (2000). Briefly, 1 ml of extract was mixed with 1 ml of sodium phosphate buffer (pH 6.6) and 1ml of potassium ferricyanide. Reactive mixture was incubated at 50 °C for 20 min. Then, 1 ml of 10% trichloroacetic acid was added, and the mixture centrifuged. Then, 1 ml distilled water, and 0.2 ml of 0.1% ferric chloride solution was added to 1 ml of the supernatant, and the absorbance was measured at 700 nm. The calibration graph was constructed by solutions of ascorbic acid (AA). The total reduction power of plant extracts was expressed as AA equivalents per g of plant wet weight (mg AAE/g WW) in accordance with the method of Augustus et al. (2015).
The electrophoretic analysis was performed in accordance with the method of Laemmly (1970) in 13.5% acrylamide gel. Protein samples (50 ^g/ml) were boiled for 5 min at 95 °C and applied to the respective wells in the gel. Analysis was run at 20 mA in the electrophoresis unit VE-20 (Russia). Protein standards were run in parallel with the samples. Bands were stained with 0.3% Coomassie Brilliant Blue G-250.
Data obtained were expressed as mean ± standard deviation (SD) of three measurements. Statistical analyses were performed with ANOVA. Correlation analysis examined the interrelations between the study indexes of the metabolite's content and the reducing power (P < 0.05). Tukey's criterion of an honestly significant difference (HSD) in group mean was used to distinguish between samples of mean values (Mcdonald, 2014). This criterion makes it possible to conduct multiple pair comparisons of mean values. Differences were considered statistically significant at Р < 0.05.
Results
The highest catalase (CAT) activity in the leaves of all Chaenomeles species was found in April, then declining until June in leaves of Ch. japonica var. maulei and hybridogenic species Ch. * superba and Ch. * californica (P < 0.01), or until July in all other species (Table 2). In the leaves of all species, catalase activity increased sharply in August relative to July. In August, the activity of catalase in the leaves of all species reached 39-74% of the level in April (P <
0.01), excluding the species Ch. japonica var. maulei , in the leaves of which the enzyme activity in August exceeded the April index by 2.3 times (P < 0.01).
Dynamics of the peroxidase activity in the leaves of Chaenomeles plants had species-specific features. The activity of plant benzidine peroxidase (BPO) increased in May in comparison with April only in the leaves of species Ch. cathayensis (by 62%, P < 0.05), whereas in the leaves of all other species the enzyme activity decreased reaching only 33-46% (P < 0.05) of the April level (Table 3). In June, a noticeable activation of benzidine peroxidase was revealed in leaves of all Chaenomeles plants, especially significant in the species Ch. japonica, Ch. japonica var. maulei, and Ch. x californica (in 2.0, 2.5 and 3.2 times respectively, P < 0.05). In July, the increase in enzymatic activity continued only in the leaves of Ch. cathayensis, whereas in the leaves of other species the BPO activity decreased in comparison with June, especially in both Japanese species (by 37% and 25%, respectively, P < 0.05).
The activity of guaiacol peroxidase (GPO) in plant leaves in May exceeded the April level in all Chaenomeles species, except for Ch. japonica var. maulei and Ch. x superba, where the activity decreased slightly (Table 4). In the leaves of Ch. cathayensis and Ch. japonica the highest GPO activity was found in May, exceeding the April level by 3.2 and 1.3 times, respectively (P < 0.01), whereas in leaves of all other species the highest enzyme activation was revealed in June. In August, the leaves of all species, excluding Ch. cathayensis, had high activity of guaiacol peroxidase, exceeding the April level by 11-12%, or even more than twice in the leaves of species Ch. japonica var. maulei and Ch. x californica (P < 0.01). The total phenolic compounds in the leaves of Chaenomeles species as determined by Folin - Ciocalteau assay, was expressed as gallic acid equivalents (GAE) by reference to a standard curve (y = 304.15x, and R2 = 0.99). The total flavonoids content in leaves of various species of Chaenomeles was determined using aluminum chloride method, and is expressed as rutin equivalent (RE) (equation of regression y = 0.0139x, R2 = 0.999). The total reducing power (RP) of the leaves of Chaenomeles different species was expressed as the equivalent of ascorbic acid (AAE) (equation of linear regression y = 252.05x -32.316, R2 = 0.993). In the leaves of studied plants, the total content of phenols varied notably, while flavonoids content did not differ in variety (Table 5). The content of the phenolic compounds as well as the flavonoids content in the ripe fruits of all Chaenomeles species (Table 3) was lower than in the leaves of studied plants (Table 6). Electrophoretic analysis of the proteins from plant seeds of the genus Chaenomeles revealed a wide spectrum of the polypeptides in the molecular weight range 9.6-83.2 kD (Fig. 1).
Table 2
Changes of catalase activity in leaves of the Chaenomeles plants during vegetation (x ± SD, n = 3, P < 0.01)
Species Catalase (CAT) activity, ^M H2O2/S g WW
April May June July August
Ch. speciosa 25.34 ± 0.33a 19.23 ± 0.09b 1.06 ± 0.09cd 0.48 ± 0.01cd 14.71 ± 0.56e
Ch. cathayensis 32.41 ± 0.40a 21.75 ± 0.09be 0.80 ± 0.16cd 0.65 ± 0.01cd 20.70 ± 1.87be
Ch. japonica 35.00 ± 1.05a 9.23 ± 0.12b 0.89 ± 0.08cd 0.48 ± 0.01cd 26.30 ± 0.98e
Ch. japonica var. maulei 16.88 ± 0.66a 9.71 ± 0.08b 0.89 ± 0.08c 3.14 ± 0.24d 38.20 ± 0.96e
Ch. x superba 31.38 ± 0.58a 11.32 ± 0.16be 1.44 ± 0.40c 3.15 ± 0.05d 12.08 ± 0.10be
Ch. x californica 24.22 ± 0.32a 5.01 ± 0.10b 0.18 ± 0.01cd 0.92 ± 0.09cd 13.35 ± 0.81e
Note: the same letters indicate statistically insignificant differences in the means of the compared pair according to Tukey's criterion (HSD).
Table 3
Changes of benzidine-peroxidase activity in the leaves of Chaenomeles plants during vegetation (Mean ± SD, n = 3, P < 0.05)
Species Benzidine-peroxidase (BPO) activity, mM benzidine/min g WW
April May June July August
Ch. speciosa 181.59 ± 0.29a 83.99 ± 1.38b 147.94 ± 2.32c 121.81 ± 3.38d 114.90 ± 4.75de
Ch. cathayensis 117.20 ± 0.99a 190.34 ± 1.24b 195.28 ± 2.20bc 214.45 ± 3.46d 89.28 ± 5.65e
Ch. japonica 159.43 ± 6.52a 65.93 ± 0.32b 130.33 ± 3.40c 82.26 ± 3.44d 157.46 ± 5.95ae
Ch. japonica var. maulei 104.24 ± 1.39a 38.75 ± 1.58b 94.08 ± 2.77c 73.37 ± 2.67d 129.92 ± 6.39e
Ch. x superba 188.69 ± 3.36a 63.17 ± 1.01b 119.88 ± 0.79c 108.52 ± 3.48d 145.21 ± 7.71e
Ch. x californica 149.22 ± 1.20a 55.00 ± 1.19b 177.53 ± 1.58c 151.22 ± 2.93ad 153.11 ± 5.55ade
Note: see Table 2.
Table 4
Changes of guaiacol-peroxidase activity in the leaves of Chaenomeles plants during vegetation (x ± SD, n = 3, P < 0.01)
Species
Guaiacol-peroxidase (GPO) activity, mM guaiacol/min g WW
April
May
June
July
August
Ch. speciosa 16.58 ± 0.10a 25.80 ± 1.19b 26.81 ± 0.96bc 16.56 ± 1.30ad 19.40 ± 0.93e
Ch. cathayensis 16.34 ± 0.02a 51.53 ± 2.19b 42.86 ± 0.60bc 30.03 ± 1.67d 10.27 ± 0.41e
Ch. japonica 15.54 ± 0.23a 20.48 ± 1.53b 16.05 ± 0.35ac 15.37 ± 0.62acd 16.27 ± 0.72ade
Ch. japonica var. maulei 13.86 ± 0.93a 12.84 ± 1.10* 27.89 ± 0.39c 14.87 ± 0.94a" 31.55 ± 1.48e
Ch. x superba 20.88 ± 0.23a 17.71 ± 1.17b 20.75 ± 0.72ac 17.34 ± 1.03bd 24.00 ± 0.73e
Ch. x californica 12.46 ± 0.15a 14.41 ± 0.70ab 54.91 ± 0.17 21.78 ± 1.07d 32.71 ± 2.40e
Note: see Table 2. Table 5
Total phenolic, total flavonoids content and the reducing power in the leaves of Chaenomeles plants
Total phenolic Total flavonoids Total reducing
Species content, mg content, mg power, mg
GAE/100 g WW RE/100 g WW AAE/100 g WW
Ch. speciosa 2928.0 ± 142.2 274.2 ± 9.8 772.0 ± 10.5
Ch. cathayensis 4355.2 ± 119.3 275.9 ± 8.9 1137.9 ± 47.5
Ch. japonica 4307.1 ± 111.3 273.5 ± 11.4 1054.7 ± 30.5
Ch. japonica var. maulei 4017.3 ± 105.6 263.5 ± 10.2 1057.3 ± 37.2
Ch. x superba 4476.1 ± 122.2 274.3 ± 9.0 1157.2 ± 37.8
Ch. x californica 5277.0 ± 132.3 285.1 ± 9.3 1413.6 ± 33.8
Table 6
Total phenolic, total flavonoids content and the reducing power in Chaenomeles fruits
Total phenolic Total flavonoids Total reducing
Species content, mg content, mg power, mg
GAE/100 g WW RE/100 g WW AAE/100 g WW
Ch. speciosa 1784.4 ± 98.1 29.8 ± 2.1 565.8 ± 15.7
Ch. cathayensis 3331.7 ± 127.3 66.5 ± 4.6 1121.7 ± 27.5
Ch. japonica 2869.2 ± 81.8 38.4 ± 3.3 885.6 ± 19.5
Ch. japonica var. maulei 2783.0 ± 75.2 62.6 ± 5.1 872.3 ± 21.9
Ch. x superba 2676.5 ± 118.8 56.8 ± 3.6 784.8 ± 16.8
Ch. x californica 2636.0 ± 89.1 41.9 ± 2.9 937.7 ± 22.3
Fig. 1. Electrophoretic spectra (SDS-PAGE) of the seed proteins of the Chaenomeles plants: M- protein markers (cytochrome C, 12.0 kD; carbonic anhydrase, 29.5 kD; ovalbumin, 45.5 kD; bovine serum albumin, 67.0 kD); 1 - Ch. japonica; 2 - Ch. cathayensis; 3 - Ch. x superba; 4 - Ch. speciosa
The number of protein bands in the spectra varied from 16 in the seeds of species Ch. japonica to 19 bands in the seeds of Ch. speciosa and Ch. cathayensis, and to 22 in the seeds of the hybrid Ch. x superba.
Dominant components in the protein complex of seeds were also different in the species from different regions of origin. The polypeptides with a molecular weight of 19.7 and 29.3 kD predominated in the seeds of Ch. speciosa, and the components of 18.1, 29.3 and 38.7 kD dominated in the composition of the seed proteins of Ch. cathayensis. In the seeds of Ch. japonica fruits, the components with a molecular weight of 18.1, 31.6 and 46.0 kD were most expressed.
Discussion
The functioning of catalase is the main metabolic pathway to protect the photosynthetic process, eliminating excess hydrogen peroxide, which is formed in plant cells under the influence of unfavourable environmental conditions (Huseynova et al., 2015). The effect of drought, high temperature and intense sunlight on plants reduces the effectiveness of photosynthesis, instead reinforcing the process of photorespiration and causing excessive accumulation of reactive oxygen species, including hydrogen peroxide (Luna et al., 2005). In our study, high catalase activity over a long period (from April to June) was noted in the leaves of species Ch. cathayensis and Ch. speciosa that originate from the mountainous regions of China and are more tolerant of drought (Table 2). In the hybridogenic species Ch. x cali-fornica and Ch. x superba, as well as in Ch. japonica var. maulei, the catalase activity in leaves declined gradually in the interval from April to June, but increased sharply in July, indicating a moderate tolerance of these species to the arid character of the regional climate. In the leaves of Ch. japonica catalase activity was maximal in April, quite high in May, but significantly decreased in June and July, against the background of the effect of high temperature and a decrease in air humidity.
The functions of peroxidases in the plant cells are diverse and numerous, including participation in eliminating excess hydrogen peroxide (Halliwell, 2006) and in the phenols metabolism (Allison & Schultz, 2004). At the same time, the activation of benzidine peroxidase and guaiacol peroxidase under the action of water deficiency can be more likely associated with the involvement of enzymes in the lignification process than with the protection of plants from oxidative stress (Lee et al., 2007). Among the Chaenomeles plants, the highest activity of benzidine peroxidase was found in both species of Chinese origin and hybridogenic species Ch. x californica and Ch. x superba (Table 3). Whereas, in leaves of Ch. cathayensis the enzyme activity increased significantly until August, in other species BPO activity declined sharply in May, but then increased significantly, remaining high during the period of the greatest increase in drought and heat. In both Japanese species, the activity of benzidine peroxidase in the leaves was reduced during the vegetation, reaching a high level only in August, indicating a lower tolerance of these species. Higher activity of guaiacol peroxidase at the beginning of vegetation and the most significant activation of the enzyme during the growing season were found in the leaves of Ch. cathayensis, Ch. speciosa and Ch. x californica (Table 4). The activity of GPO in the leaves of both Japanese species was less significant during the vegetation, remaining at the same level in August in Ch. japonica leaves, while increasing more than twice in the leaves of Ch. japonica var. maulei.
The health promoting abilities of fruit plants are largely determined by the high antioxidant capacity of plant metabolites, which can be realized as free radical scavenging activity, and by reduction or
chelation of metals as well (Costa et al., 2009; Du et al., 2013). In the present paper, the antioxidant level of the Chaenomeles leaves and fruits was estimated as the total reducing power (TRP) and expressed as the ascorbic acid equivalents. Leaves of Ch. * californica had the highest TRP (14.1 mg AAE/g WW), while reducing power in leaves of Ch. speciosa was almost two times less (Table 5). Leaves of hybri-dogenic species Ch. * superba and Ch. cathayensis as well showed high reduction ability; whereas it was slightly lower in leaves of both Japanese species. However, in general, the total reducing power of Chaenomeles leaves can be estimated as substantial, given that reducing power of Thymus vulgaris leaves reached 0.94 mg AAE/g WW only (Eghdami et al., 2013), and the total reducing capacity of Cype-rus erectus plants (20.0 mg AAE/g DW) was estimated as high (Augustus et al., 2015). The study results indicate that leaves of the Chae-nomeles species can be considered as plant raw materials with high antioxidant activity. The output coincides with the data of Han et al. (2016) on the radical scavenging activities and anti-inflammatory effects of different Ch. sinensis leaf extracts.
The total reducing power in the fruit flesh of Chaenomeles species was lower as compared to leaves, and Ch. cathayensis fruits showed the maximal level while Ch. speciosa the minimal, respectively, 11.2 and 5.7 mg AAE/g WW (Table 6). The antioxidant capacity of Ch. japonica and Ch. japonica var. maulei fruits occupied the middle position between the TRP of the hybridogenic species Ch. * superba and Ch. * californica (7.9 and 9.4 mg AAE/g respectively). In total, the antioxidant potential of Chaenomeles fruits is comparable to the level shown by Mitic et al. (2016) for other fruit crops, including nectarine (14.36-17.58 mg AAE/g), plum (32.43-58.30 mg AAE/g), and peach (12.31-24.55 mg AAE/g) as well as to the reducing power of Berberis fruits defined in the range 5.0-9.6 mg AAE/100 g DW (Khromykh et al., 2018).
Polyphenols are abundantly present in a majority of fruits, herbs and vegetables, and can play the protective role against reactive oxygen and nitrogen species, UV light, plant pathogens, parasites and predators (Brglez et al., 2016). These secondary plant metabolites represent a large and diverse group of substances including simple polyphenols, catechins, anthocyanins, glycosides of flavones, flavonols, isoflavones and flavanones and other compounds (Sakakibara et al., 2003; Jafari et al., 2014). In our study, the total phenolic content in the Chaenomeles fruits isopropanol extracts was the lowest in Ch. speciosa (17.84 mg GAE/g WW), average in Ch. japonica and Ch. japonica var. maulei (28.69 and 27.83 mg GAE/g), and the highest (33.32 mg GAE/g) in Ch. cathayensis fruits (Table 6). As for the hybridogenous species Ch. * californica and Ch. * superba, the content of phenols in their fruits reached an average level relative to the parent plants. The results obtained differ from the data on the phenol content in the acetone extracts from the fruits of Ch. speciosa (46.92 mg GAE/g) and Ch. japonica (19.35 mg GAE/g) collected in the Botanical Garden of Beijing in China (Du et al., 2013). It is obvious that the differences in the accumulation of antioxidant metabolites in the fruits of the same plant species are due to the influence of environmental conditions, which vary widely in these two regions. The study results suggest also that the adaptation of Japanese and Chinese species to the soil and semiarid climatic conditions of the Steppe Dnieper was accompanied by more significant accumulation of the antioxidant compounds in the fruits of Ch. japonica plants than Ch. speciosa. This assumption agrees with the data of Nichols et al. (2015) that the high level of phenolic compounds accumulation positively correlated with the improvement of the stability of clover plants to drought. In general, the content of phenolic compounds in the Chaenomeles fruits significantly exceeded those for some other fruit crops, including peach (0.69-4.00 mg GAE/g), nectarine (0.93-1.83 mg GAE/g), and plum (3.91-13.53 mg GAE/g), reported by Mitic et al. (2016). In the fruits of different apple varieties cultivated in Italy the mean content of total polyphenols lay between 66.2 and 211.9 mg/100 g of FW (Vrhovsek et al., 2004), while other data (Wojdylo et al., 2008) showed the variation in the range from 523 to 2,724 mg GAE/100 g DW in a group of 67 different apples varieties.
The total phenolic content in leaves of Chaenomeles plants measured during the flowering exceeded that in the fruits of each species,
and varied notably (Table 5). The most polyphenols accumulation was revealed in leaves of hybridogenic species Ch * oalifomica (52.8 mg GAE/g WW), while the lowest was in Ch. speciosa leaves (29.3 mg GAE/g). Variability in the accumulation of phenols (40.7-160.0 mg GAE/g) was established in leaves of Marrubium vulgare plants from different geographical origins (Bouterfas et al., 2016). A similarly wide range of the polyphenols accumulation (11.19-51.49 mg GAE/g) in Curcuma longa plants from different ecological niches of Western Himalayas was reported by Arya et al. (2015). Thus, the variability of the secondary metabolites accumulation in Chaenomeles leaves and fruits is an indicator of the adaptation success of introduced species from the different geographical origin to climatic conditions of the Steppe Dnieper.
Flavonoids are a subclass of the polyphenols, and they form a large group of food constituents, many of which alter metabolic processes and have a positive impact on health (Beecher, 2003), possess preventive and therapeutic potential (Owczarek et al., 2017). In the leaves of Chaenomeles plants, the content of flavonoids varied very slightly, ranging from 2.64 mg RE/g WW in the species Ch. japonica var. maulei to 2.85 mg RE/g in Ch * californica (Table 5). As for the plant fruits, the highest flavonoids accumulation was revealed in Ch. cathayensis and Ch. japonica var. maulei (0.67 and 0.63 mg RE/g WW respectively), while the lowest level (0.30 mg RE/g WW) was in the fruits of Ch. speciosa (Table 6). The fruits of Japanese quince and the hybrid species Ch. * superba and Ch. * californica had an intermediate flavonoid content, respectively, 0.38, 0.57, and 0.42 mg RE/g WW. The results obtained are consistent with the data (Hafez-Taghva et al., 2016) on the accumulation of flavonoids in the range 0.091-0.618 mg RE/g in Ch. japonica fruits. Dissimilar levels of the flavonoids accumulation in fruits of different species can be attributed to the general diversity of the phenolic compounds, which was found within the genus Chaenomeles (Watychowicz et al., 2017; Miao et al., 2016; Lewandowska et al., 2013).
Correlation analysis revealed the positive relationship between the total reducing power and the total phenols content, and total flavonoids content in leaves of the Chaenomeles plants (respectively, r = 0.96 and r = 0.88, P < 0.05). Consequently, phenols in general, and flavonoids in particular, make an equally significant contribution to the overall high antioxidant activity of the leaves. As for the fruits of the Chaenomeles species, strong correlation was established between the total reducing power and the total phenols content (r = 0.95, P < 0.05), whereas a lower correlation coefficient (r = 0.66) was found between the total reducing power and total flavonoids content. The explanation for the low level of correlation may be due to the fact that flavonoids are widespread in nature, but not evenly (Beecher, 2003), most likely even within the same genus of plants.
Storage proteins make up the bulk of the seed protein and play an important role in their quality, as the success of germination and further growth of the seedlings in extreme environmental conditions largely depends on their content, rate of transformation and use in the metabolic processes (Radhika & Rao, 2015). The polypeptide composition of the storage proteins of Ch. japonica seeds had fewer components than the seeds of species from the Chinese region, Ch. speciosa and Ch. cathayensis (Fig. 1). Consequently, the distinction of seeds polypeptide composition of the Chaenomeles species from different regions can make a significant contribution to the growth and resistance of these species in their natural areas and in new habitats as well. This assumption is consistent with the data (Bartish et al., 2000; Rumpunen, 2002) that the species Ch. japonica differs clearly from Ch. speciosa and Ch. cathayensis in the aspects related to spontaneous hybridization. The differences in the polypeptide composition of seed storage proteins serve as yet further evidence of the wider range of adaptive capabilities of the Chaenomeles species of Chinese origin to the conditions of the steppe climate.
Conclusion
A comparative study of the antioxidant enzymes activity and the second metabolites accumulation in leaves and fruits of several natural and hybridogenic genotypes of the genus Chaenomeles were car-
ried out. Differences in the antioxidant capacity of plants due to the peculiarities of Chinese and Japanese species adaptation to the conditions of steppe climate have been established. In leaves, the highest activity of antioxidant enzymes and their greatest activation under the influence of heat and drought during vegetation were found in Ch. cathayensis and Ch. speciosa, and the lowest in leaves of both Japanese species. The total antioxidant capacity and phenolic content reached the highest level in the leaves of hybridogenic species Ch. x superba and Ch. x californica, as well as Ch. cathayensis. In general, the total antioxidant capacity and metabolite content in the fruits of all Chaenomeles species was found to be lower compared to leaves. The total reducing power and the accumulation of metabolites were the greatest in fruits of Ch. cathayensis, while Ch. speciosa fruits had unexpectedly low levels. The significant contribution of phenolic compounds to the antioxidant capacity of leaves and fruits was confirmed by high correlation coefficients. The study results showed that leaves and fruits of all Chaenomeles species are sufficiently rich sources of natural antioxidants. Nevertheless, the specific differences established in the work led to the conclusion that species Ch. cathayensis and Ch. x californica are the most promising species for cultivation in the steppe zone in order to obtain fruits with a rich content of antioxidant compounds.
References
Alexeyeva, A. A., Lykholat, Y. V., Khromykh, N. O., Kovalenko, I. M., & Bo-roday, E. S. (2016). The impact of pollutants on the antioxidant protection of species of the genus Tilia at different developmental stages. Visnyk of Dnipropetrovsk University. Biology, Ecology, 24(1), 188-192. Allison, S. D., & Schultz, J. C. (2004). Differential activity of peroxidase isozy-mes in response to wounding, gypsy moth, and plant hormones in Northern red oak (Quercus rubra L.). Journal of Chemical Ecology, 30(7), 1363-1379.
Arya, N., Prakash, O., Verma, A. K., & Pant, A. K. (2015). Variation in antioxidant potential of Curcuma longa L. collected from different ecological niches of Western Himalayan region. International Journal of Pharmacy and Pharmaceutical Sciences, 7(7), 85-90. Augustus, O. K., Janet, J. O., Ebenezer, T. B., & Ogboma, U. J. (2015). Anti-oxidant activities, total flavonoid and total phenolic contents of whole plant of Kyllinga erecta Shumach. Journal of Food and Nutrition Research, 3(8), 489-494. Baranowska-Bosiacka, I., Bosiacka, B., Rast, J., Gutowska, I., Wolska, J., Rbacz-Maron, E., DKbia, K., Janda, K., Korbecki, J., & Chlubek, D. (2017). Macro- and microelement content and other properties of Chaenomeles japonica L. fruit and protective effects of its aqueous extract on hepatocyte metabolism. Biological Trace Element Research, 178(2), 327-337. Bartish, I. V., Garkava, L. P., Rumpunen, K., & Nybom, H. (2000). Phylogene-tic relationships and differentiation among and within populations of Chaenomeles Lindl. (Rosaceae) estimated with RAPDs and isozymes. Theoretical and Applied Genetics, 101, 554-563. Beecher, G. R. (2003). Overview of dietary flavonoids: Nomenclature, occurrence and intake. The Journal of Nutrition, 133(10), 3248S-3254S. Bouterfas, K., Mehdadi, Z., Elaoufi, M. M., Latreche, A., & Benchiha, W. (2016). Antioxidant activity and total phenolic and flavonoids content variations of leaves extracts of white Horehound (Marrubium vulgare Linné) from three geographical origins. Annales Pharmaceutiques Francaises, 74(6), 453-462.
Brglez, M. E., Knez, H. M, Skerget, M., Knez, Z., & Bren, U. (2016). Polyphenols: Extraction methods, antioxidative action, bioavailability and anticar-cinogenic effects. Molecules, 21(7), e901. Cheynier, V. (2005). Polyphenols in foods are more complex than often
thought. The American Journal of Clinical Nutrition, 81(1), 223S-229S. Costa, R. M., Magalhres, A. S., Pereira, J. A., Andrade, P. B., Valentro, P., Carvalho, M., & Silva, B. M. (2009). Evaluation of free radical-scavenging and antihemolytic activities of quince (Cydonia oblonga) leaf: A comparative study with green tea (Camellia sinensis). Food and Chemical Toxicology, 47(4), 860-865. Du, H., Wu, J., Li, H., Zhong, P. X., Xu, Y. J., Li, C. H., Ji, K. X., & Wang, L. S. (2013). Polyphenols and triterpenes from Chaenomeles fruits: Chemical analysis and antioxidant activities assessment. Food Chemistry, 141(4), 4260-4268.
Eghdami, A., Eizadi, M., & Sadeghi, F. (2013). Polyphenolic content and antioxidant activity of hydroalcohlic and alcoholic extract of Thymus vulgaris. Journal of Biodiversity and Environmental Sciences, 3(5), 94-101.
Gorlach, S., Wagner, W., PodsKdek, A., Szewczyk, K., Kozioikiewicz, M., & Dastych, J. (2011). Procyanidins from Japanese quince (Chaenomeles japonica) fruit induce apoptosis in human colon cancer Caco-2 cells in a degree of polymerization-dependent manner. Nutrition and Cancer, 63(8), 1348-1360.
Górnas, P., Siger, A., Juhnevica, K., Lacis, G., Sne, E., & Seglina, D. (2014). Cold-pressed Japanese quince (Chaenomeles japonica (Thunb.) Lindl. ex Spach) seed oil as a rich source of a-tocopherol, carotenoids and pheno-lics: A comparison of the composition and antioxidant activity with nine other plant oils. European Journal of Lipid Science and Technology, 116(5), 563-570.
Góth, L. (1991). A simple method for determination of serum catalase activity and revision of reference range. Clinica Chimica Acta, 196(2-3), 143-151.
Gregory, R. P. F. (1966). A rapid assay for peroxidase activity. Biochemical Journal, 101(3), 582-583.
Hafez-Taghva, P., Zamzad, M., & Khalafi, L. (2016). Total flavonoid content and essential oil composition of Chaenomeles japonica (Thunb.) Lindl. ex Spach from North of Iran. Indian Journal of Natural Products and Resources, 7(1), 90-92.
Halliwell, B. (2006). Reactive species and antioxidants. Redox biology is a fundamental theme in aerobic life. Plant Physiology, 141, 312-322.
Hamauzu, Y., Inno, T., Kume, C., Me, M., & Hiramatsu, K. J. (2006). Antioxidant and antiulcerative properties of phenolics from Chinese quince, quince, and apple fruits. Journal of Agricultural and Food Chemistry, 54(3), 765-772.
Han, Y. K., Kim, Y. S., Natarajan, S. B., Kim, W. S., Hwang, J. W., Jeon, N. J., Jeong, J. H., Moon, S. H., Jeon B. T., & Park P. J. (2016). Antioxidant and anti-inflammatory effects of Chaenomeles sinensis leaf extracts on LPS-stimulated RAW 264.7 Cells. Molecules, 21(4), 422.
Huseynova, I. M., Aliyeva, D. R., Mammadov, A. C., & Aliyev, J. A. (2015). Hydrogen peroxide generation and antioxidant enzyme activities in the leaves and roots of wheat cultivars subjected to long-term soil drought stress. Photosynthesis Research, 125, 279-289.
Jafari, S., Saeidnia, S., & Abdollahi, M. (2014). Role of natural phenolic compounds in cancer chemoprevention via regulation of the cell cycle. Current Pharmaceutical Biotechnology, 15(4), 409-421.
Khromykh, N. O., Lykholat, Y. V., Kovalenko, I. M., Kabar, A. M., Didur, O. O., Nedzvetska, M. I. (2018). Variability of the antioxidant properties of Berberís fruits depending on the plant species and conditions of habitat. Regulatory Mechanisms in Biosystems, 9(1), 56-61.
Laemmly, U. K. (1970). Cleavage of structural of bacteriophage T-4. Nature, 227, 680-685.
Lee, B.-R., Kim, K.-Y., Jung, W.-J., Avice, J.-C., Ourry, A., & Kim, T.-H. (2007). Peroxidases and lignification in relation to the intensity of water-deficit stress in white clover (Trifolium repens L.). Journal of Experimental Botany, 58(6), 1271-1279.
Lewandowska, U., Szewczyk, K., Owczarek, K., Hrabec, Z., PodsKdek, A., Kozioikiewicz, M., & Hrabec, E. (2013). Flavanols from Japanese quince (Chaenomeles japonica) fruit inhibit human prostate and breast cancer cell line invasiveness and cause favorable changes in Bax/Bcl-2 mRNA ratio. Nutrition and Cancer, 65(2), 273-285.
Luna, C. M., Pastori, G. M., Driscoll, S., Groten, K., Bernard, S., & Foyer, C. H. (2005). Drought controls on H2O2 accumulation, catalase (CAT) activity and CAT gene expression in wheat. Journal of Experimental Botany, 56(411), 417-423.
Ma, B., Wang, J., Tong, J., Zhou, G., Chen, Y., He, J., & Wang, Y. (2016). Protective effects of Chaenomeles thibetica extract against carbon tetra-chloride-induced damage via the MAPK/Nrf2 pathway. Food and Function, 7(3), 1492-1500.
Mcdonald, J. H. (2014). Handbook of biolological statistics. Sparky House Publishing, Baltimore.
McGhie, T. K., Hunt, M., & Barnett, L. E. (2005). Cultivar and growing region determine the antioxidant polyphenolic concentration and composition of apples grown in New Zealand. Journal of Agricultural and Food Chemistry, 53(8), 3065-3070.
Miao, J., Zhao, C., Li, X., Chen, X., Mao, X., Huang, H., Wang, T., & Gao, W. (2016). Chemical composition and bioactivities of two common Chaeno-meles fruits in China: Chaenomeles speciosa and Chaenomeles sinensis. Journal of Food Science, 81(8), H2049-2058.
Mitic, V., Ilic, M., Dimitrijevic, M., Cvetkovic, J., Ciric, S., & Stankov-Jovano-vic, V. (2016). Chemometric characterization of peach, nectarine and plum cultivars according to fruit phenolic content and antioxidant activity. Fruits, 7(1), 57-66.
Mojzer, E. B., Hrncic, M. K., Skerget, M., Knez, Z., & Bren, U. (2016). Polyphenols: Extraction methods, antioxidative action, bioavailability and anti-carcinogenic effects. Molecules, 21(7), e901.
Nichols, S. N., Hofmann, R. W., & Williams, W. M. (2015). Functional roles of secondary metabolites in plant-environment interactions. Physiological
drought resistance and accumulation of leaf phenolics in white clover interspecific hybrids. Environmental and Experimental Botany, 119, 40-47.
Nwanna, E. E., Ibukun, E. O., & Oboh, G. (2013). Inhibitory effects of metha-nolic extracts of two eggplant species from South-Western Nigeria on starch hydrolysing enzymes linked to type-2 diabetes. African Journal of Pharmacy and Pharmacology, 7(23), 1575-1584.
Owczarek, K., Hrabec, E., Fichna, J., Sosnowska, D., Koziolkiewicz, M., Szy-manski, J., & Lewandowska, U. (2017). Flavanols from Japanese quince (Chaenomeles japonica) fruit suppress expression of cyclooxygenase-2, etalloproteinase-9, and nuclear factor-kappaB in human colon cancer cells.
Acta Biochimica Polonica, 64(3), 567-576.
P^kal, A., & Pyrzynska, K. (2014). Evaluation of aluminium complexation reaction for flavonoid content assay. Food Analytical Methods, 7, 1776-1782.
Pulido, R., Bravo, R. L., & Saura-Calixto, F. (2000). Antioxidant activity of dietary polyphenols as determined by a modified ferric reducing/antioxidant power assay. Journal of Agricultural and Food Chemistry, 48, 3396-3402.
Radhika, V., & Rao, V. S. H. (2015). Computational approaches for the classification of seed storage proteins. Journal of Food Science and Technology, 52(7), 4246-4255.
Ranieri, A., Castagna, A., Baldam, B., & Soldatini, G. F. (2001). Iron deficiency differently affects peroxidase isoforms in sunflower. Journal of Experimental Botany, 52(354), 25-35.
Ros-Garciaa J. M., Sanchez J. L., Hellin P., Jordan, M. J., Vila, R., Rumpunen, K. (2004). Characterization of juice in fruits of different Chaenomeles species. LWT-Food Science and Technology, 37(3), 301-307.
Rumpunen, K. (2002). Chaenomeles: Potential new fruit crop for Northern Europe. In: Janick, J., & Whipkey, A. (Eds.). Trends in new crops and new uses. ASHS Press, Alexandria. Pp. 385-392.
Sahan, Y., Cansev, A., Celik, G., & Cinar, A. (2012). Determination of various chemical properties, total phenolic content, antioxidant capacity and organic acids in Laurocerasus officinalis fruits. Acta Horticulturae, 939, 359-366.
Sakakibara, H., Honda, Y., Nakagawa, S., Ashida, H., & Kanazawa, K. (2003). Simultaneous determination of all polyphenols in vegetables, fruits, and teas. Journal of Agricultural and Food Chemistry, 51(3), 571-581.
Shcherbyna, R. O., Danilchenko, D. M., Parchenko, V. V., Panasenko, O. I., Knysh, E. H., Hromyh, N. A., & Lyholat, Y. V. (2017). Studying of 2-((5-R-4-R1-4h-1,2,4-Triazole-3-YI0Thio)acetic acid salts on growth and progress of Blackberries (KIOWA Variety) Propagules. Research Journal of Pharmaceutical, Biological and Chemical Sciences, 8(3), 975-979.
Singleton, V. L., Orthofer, R., & Lamuela-Raventos, R. M. (1999). Analysis of total phenols and other oxidation substrates and antioxidants by means of Folin-Ciocalteau reagent. Methods in Enzymology, 299, 152-178.
Strugala, P., Cyboran-Mikolajczyk, S., Dudra, A., Mizgier, P., Kucharska, A. Z., Olejniczak, T., & Gabrielska, J. (2016). Biological activity of japanese quince extract and its interactions with lipids, erythrocyte membrane, and human albumin. The Journal of Membrane Biology, 249(3), 393-410.
Suh, W. S., Park, K. J., Kim, D. H., Subedi, L., Kim, S. Y., Choi, S. U., & Lee, K. R. (2017). A biphenyl derivative from the twigs of Chaenomeles spe-ciosa. Bioorganic Chemistry, 72, 156-160.
Vrhovsek, U., Rigo, A., Tonon, D., & Mattivi, F. (2004). Quantitation of polyphenols in different apple varieties. Journal of Agricultural and Food Chemistry, 52(21), 6532-6538.
Watychowicz, K., Janda, K., Jakubczyk, K., & Wolska, J. (2017). Chaenomeles - health promoting benefits. Roczniki Panstwowego Zakladu Higieny, 68(3), 217-227.
Wojdylo, A., Oszmiañski, J., & Laskowski, P. (2008). Polyphenolic compounds and antioxidant activity of new and old apple varieties. Journal of Agricultural and Food Chemistry, 56(15), 6520-6530.
Zaklos-Szyda, M., & Pawlik, N. (2018). Japanese quince (Chaenomeles japonica L.) fruit polyphenolic extract modulates carbohydrate metabolism in HepG2 cells via AMP-activated protein kinase. Acta Biochimica Polonica, 65(1), 67-78.
Zhang, S., Han, L., Zhang, H., & Xin, H. (2014). Chaenomeles speciosa: A review of chemistry and pharmacology. Biomedical Reports, 2(1), 12-18.
