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15DOI: https://doi.org/10.21323/2414-438X-2021-6-4-294-299
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Available online at https://www.meatjournal.ru/jour Original scientific article Open Access
IMPACT OF FEED SUPPLEMENTATION WITH BALSAM POPLAR BUDS ON PERFORMANCE OF YOUNG BULLS
Received 25.08.2021 Accepted in revised 16.09.2021 Accepted for publication 30.10.2021
Yuriy Balji1, Martin Knicky2*
1 S. Seifullin Kazakh Agro Technical University, Nur-Sultan, Kazakhstan 2 RISE Research Institutes of Sweden, Uppsala, Sweden
Keywords: nutrition, additive, bioactive compounds, slaughter characteristics, meat quality Abstract
There is an urgent need to develop new strategies to minimize the environmental impact of animal production and support sustain-ability of food production and consumption. Feed additives have been for a long time used in animal nutrition to improve animal growth and performance as well as animal health. Balsam poplar plants (Populus balsamifera) is well known as a rich source of bioactive compounds with positive health effects, and might be used in agriculture as a feed additive for ruminants. The aim of the present study was to evaluate the effect of balsam poplar-based additives on growth and performance of fattening young bulls of Simmental breed. In the present study, we used 4 combinations of extract from balsam poplar buds or its components as a feed additives. The animals were given the supplements at the age of 15 months, 3 months before slaughter. The growth and slaughter characteristics of young bulls were studied. After the first and second month of feeding with dietary supplements, animals from the groups fed 10% balsam poplar buds extract and dry shredded balsam poplar buds had significantly higher live weight compared to the control animals fed a diet without any supplements (P < 0.05). At slaughter, group fed 10% balsam poplar buds extract had significantly higher live weight compared to control. Average daily gain was also greatest in that group. Major sensory as well as physical and chemical parameters were not affected by balsam poplar-based supplements (p > 0.05 for all) and were in line with regulatory meat hygiene requirements.
For citation: Balji, Yu., Knicky, M. (2021). Impact of feed supplementation with balsam poplar buds on performance of young bulls. Theory and practice of meat processing, 6(4), 294-299. https://doi.org/10.21323/2414-438X-2021-6-4-294-299.
Funding:
Financial support for this project was provided by the Ministry of Education and Science of the Republic of Kazakhstan (project number — AP08051983 "Development and implementation of multifunctional feed additives to increase the productivity of livestock animals with regard to quality and safety of livestock products").
Acknowledgements:
Yulia Sheiko is acknowledged for her involvement and help during experimental work.
Introduction
With continuous growth of world population, the major focus of agricultural sector is to ensure an adequate food supply and food security. Animal health and welfare are closely related to animal production efficiency and has therefore indirectly linked to reduced poverty, which is one of the UN sustainable development goals. Beef cattle is an important agricultural and economic resource in many countries including Kazakhstan. Thus, there is an increasing interest to improve feed conversion and increase growth rate without compromising meat quality and animal welfare. Reduction of negative environmental effects such as carbon emissions and contribute to sustainable land use is also important. Genetic selection, animal management, dietary modification, and application of new feeding techniques and use of feed additives [1-4] can enhance feed conversion and growth rate.
Nutritional needs of livestock animals with respect to energy, protein, minerals and vitamin requirements are well established. To optimize livestock production, antibiotic growth promoters had been used as dietary additive for decades. However, uncontrolled use of antibiotics contributes to increasing resistance in bacteria of human and animal origin [5]. Since 2006, marketing and use of antibiotics as growth promoters in animal production are prohibited in EU although hormonal growth promoters are still in use in US to increase daily weight gain and produce leaner meat [6]. Moreover, consumers prefer "hormone- and antibiotic-free" food because of potential health effects and environmental risks [7]. Over recent decades, much research has also been aimed to improve the quality of feed without use of hormonal growth promoters. This included research on forages and forage conservation [8], as well as the use of various supplements from natural sources [9-11].
Copyright © 2021, Balji et al. This is an open access article distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/ licenses/by/4.0/), allowing third parties to copy and redistribute the material in any medium or format and to remix, transform, and build upon the material for any purpose, even commercially, provided the original work is properly cited and states its license.
There is an increasing interest in using plant bioactive compounds to enhance ruminant health and performance [12]. For this purpose, numerous studies have attempted to use supplements with bioactive compounds or plant extracts [13,14]. Although plant bioactive compounds have lower potency compared to pharmaceuticals, they might affect physiological processes in a positive way and showed numerous benefits for animal health. The existing knowledge on the use of plant bioactive compounds as growth promoters in livestock production was recently reviewed by Valenzuela-Grijalva [15]. Balsam poplar (Populus bal-samifera) as a possible feed ingredient for cattle has been studied since they contain a wide range of bioactive compounds with antioxidant activity [16-18]. Cuttings from poplar were shown to have good digestibility [19]. Bark of aspen (Populus tremuloides), which belong to the same genus as balsam poplar, was suggested as a good feed for goats [20].
In the North Kazakhstan, there are approximately 1400 ha of balsam poplar plantations but utilization of the buds from balsam poplar is low. Moreover, poplars are early successional species with rapid growth rates. Several studies highlighted balsam poplar buds as an important source of chemically highly diverse bioactive substances such as phenolic compounds and essential oils [18,21]. These compounds are known for their biological functions but are still far from being fully investigated.
We hypothesized that the use of the Balsam poplar buds as a part of animal feed can facilitate digestion process and improve cattle growth and performance. Thus, in the present study we used extract from balsam poplar buds or its components as on growth rate and performance of Sim-mental bull.
Objects and methods
All procedures used in the study were conducted in accordance with the Code of Ethics of the World Medical Association (Declaration of Helsinki) for animal experiments (http://ec.europa.eu/environment/chemicals/lab_animals/ legislation_en.htm) and "The Guide for Care and Use of Laboratory Animals" [22]. During the experiment, all efforts were made to avoid animal suffering.
Animals and feeding
In total, 25 fattening young bulls of Simmental breed, with live weight (LW) from 295 to 307.8 kg and age of approximately 15 months were included into the study. The animals were randomly divided into 5 groups (n=5 in each group). All animals were fed a commercial mixed diet containing wheat grass, hay barley and concentrate. Additionally, each experimental group received a supplement containing extract from balsam poplar buds or its components. The group without any supplement served as a control (group 1). Group 2 received supplement with only polyethylene pellets (once, 500 g /animal). The applied polyethylene pellets are chemically inert substances and
do not provide energy. Group 3 received supplement with polyethylene pellets (once, 500 g /animal) in combination with 0.4% essential oil of balsam poplar buds (10 mL once a day for 5 days). Group 4 received supplement with polyethylene pellets (once, 500 g /animal) in combination with a 10% balsam poplar buds extract (10 mL once a day for 5 days). Group 5 received supplement with polyethylene pellets (once, 500 g /animal) in combination with dried shredded balsam poplar buds, containing 2% extract (5 g once a day per 5 days). The animals were given the supplements at the age of 15 months, 3 months (90 days) before slaughter.
Polyethylene pellets in the diet were mixed with a concentrate (1.5 kg of concentrated feed per animal) and fed animals after 18 hour fasting. The animals were slaughtered at a local slaughterhouse using standard procedures.
Measurements after slaughter
Dressing percentage was calculated by dividing the weight of warm carcass by the weight of the live animal and expressed as a percentage. Other measurements of the carcass and veterinary control were performed according to standard methods and veterinary (veterinary-sanitary) rules approved by RK Government No. 7-1/587 of June 29, 2015.
Reaction to the peroxidase (benzidine test) and pH
All laboratory analyses of meat were performed in accordance with the State Standard 23392-78 "Meat. Methods of freshness chemical and microscopic analysis".
Meat extract (2 ml) was added into the test tube, following by addition of 5 drops of 0.2% alcoholic benzidine and 2 drops of 1% hydrogen peroxide. The extract of fresh meat of healthy animals acquired a greenish-blue color, turning into brown in a few minutes. Such coloring is the evidence of peroxidase activity. The meat extract of sick, overworked, dead animals doesn't change the color.
pH of the meat extract was determined by potentiomet-ric method with the use of ionomer 827 pH Lab Metrohm.
Microscopy of smears-marks
The method is based on determining the number of bacteria and degradation of the muscle tissue by microscopy of smears-marks using a binocular microscope Mik-med-5, a mirror digital camera Nikon D5100 with Camera Control Pro2 software and digital cameras Omax A3590U ToupView software. Meat was cut into pieces with a clean scissors from the deep layers of the muscle and attached to a glass slide. Smears were dried, fixed in the flame of the burner and stained as previously described [23].
Sensory test
Sensory tests were performed in accordance with the State Standard 7269-79, which includes evaluation of meat appearance, color and smell, condition of the muscles in the section, as well as the evaluation of broth clarity and flavor.
The appearance and color of the meat samples were evaluated by visual examination. Appearance and color of the muscles in the section were studied in the deep layers of muscle tissue in the fresh meat section. At the same time, there were ascertained presence of adhesiveness and moisture on the section surface of meat by attaching piece of absorbent paper to the section.
The flavor of the surface layer of the test sample was examined by sensory analysis. The sections were made with a clean knife and the flavor in the deep layers was immediately identified.
Evaluation of broth clarity and flavor was performed as follows: 20 g of minced meat were placed into a flask, filled with 60 ml of distilled water and mixed thoroughly. Then, the flask was covered with a watch glass and placed into a boiling water bath. The flavor was examined within the process of heating up to 80-85 °C. To determine clarity, 20 ml of the broth and were transferred into the cylinder and visually adjusted its degree of clarity.
Statistical analysis
All statistical analyses were conducted with Statistical Analysis System, Version 9.4 (SAS Inst., Cary, NC, USA). The mixed model included fixed factor of treatment when evaluating the effect on live weight. Live weight at the beginning of the experiment was included as covariate when evaluating the effect of treatment on other characteristics. Comparisons between the control group fed traditional diet only, and groups with dietary supplements were performed using probability differences. A p-value less than 0.05 was regarded as statistically significant.
Results and discussion
During the experiment span, veterinarians as healthy recognized all animals, and no morbidity and mortality were recorded in any of groups.
Table 1. Growth parameters of young bulls
At the beginning of the experiment live weight of the young bulls did not significantly differ between the groups (Table 1; P = 0.974). After the first and second month of feeding with dietary supplements, animals from the groups 4 and 5 (fed 10% balsam poplar buds extract and dry shredded balsam poplar buds, respectively) had significantly higher live weight compared to the control animals (Table 1, P < 0.05). At slaughter, however, the overall effect of group did not reach the significant differences, although according to more specific probability differences test, group 4 fed 10% balsam poplar buds extract had significantly higher live weight compared to control (Table 1). Average daily gain was also greatest in this group.
The determination of the live weight at a given age is probably one of the most obvious and easiest way to access feed efficiency. To the best of our knowledge, this is the first study to investigate the effect of the Balsam poplar buds as a part of animal feed on cattle growth and performance. In the present study, only live weights of the animals in groups 4 and 5 differed from that in control group, indication that the supplements of 10% balsam poplar buds extract and dried shredded balsam poplar buds, containing 2% extract, were most promising in increasing feed efficiency. The loss of statistical significance between group 5 and control group at slaughter time suggests that possible adaptation of the animals to dried shredded balsam poplar buds. In future practical application, the time between providing supplements of balsam poplar-based product and slaughter can be reduced.
Carcass weight was greatest in the groups 2 and 4 (fed with only polyethylene pellets and fed 10% balsam poplar buds extract, respectively) (Table 2). No differences in carcass dressing percentage and other post-slaughter characteristics between treatments was observed (P > 0.05 for all, Table 2). Dressing percentage is one of factors defining the value of a livestock animal after slaughter. Numerically,
Parameter Groups P-value, effect
1 (control) 2 3 4 5 of group
Live weight before changes in the diet, kg
301 ± 13.6
304 ± 13.6
301 ± 13.6
308 ± 13.6
295 ± 13.6
0.975
Live weight after 1 month, kg 322a ± 3.4 326a ± 3.4 323a ± 3.4 338b ± 3.4 334b ± 3.4 0.015
Live weight after 2 month, kg 341a ± 6.0 356a ± 6.0 351a ± 6.0 366b ± 6.1 371b ± 6.1 0.017
Live weight at slaughter, kg 372a ± 7.9 393a ± 7.9 387a ± 7.9 401b ± 7.9 394a ± 7.9 0.139
Average daily gain, g 777a ± 87.4 1017a ± 87.5 948a ± 87.4 1103b ± 87.4 1025a ± 88.0 0.139
Data are presented as least squares (LS) means ± standard errors. LS means with different superscripts within row differs at P < 0.05
Table 2. Effect of dietary supplementation with balsam poplar buds on post-slaughter characteristics
Indicators Groups P-value, effect
1 (control) 2 3 4 5 of group
Carcass, kg 194a ± 5.7 211b ± 5.7 206a ± 5.7 218b ± 5.8 209a ± 5.8 0.086
Dressing, % 52.1 ± 1.15 53.7 ± 1.15 53.3 ± 1.15 54.4 ± 1.16 53.0 ± 1.16 0.714
Muscle, % 53.8 ± 0.97 53.3 ± 0.97 53.8 ± 0.97 52.7 ± 0.97 53.7 ± 0.97 0.918
Bones, % 19.2 ± 0.94 17.6 ± 0.94 18.4 ± 0.94 18.9 ± 0.95 18.3 ± 0.95 0.798
Connective tissue,% 11.6 ± 0.61 10.4 ± 0.61 11.0 ± 0.61 11.1 ± 0.61 11.3 ± 0.61 0.741
Fat, % 15.4 ± 0.88 18.6 ± 0.88 16.8 ± 0.88 17.2 ± 0.89 16.8 ± 0.89 0.191
Data are presented as least squares (LS) means ± standard errors. LS means with different superscripts within row differs at P < 0.05
the animals from group 1 (control) had the lowest dressing percentage even though these differences did not reach statistical significance.
The economic value of livestock animals depends on its composition. Nowadays in many countries, a progress was made in reducing the fat content of livestock animals because of consumer demand for lean meat. Generally, the relationship between live weight and fat content is affected by feeding, environmental factors as well as any subclinical diseases that might affect the animal growth rate. In the present study, the animals were kept in the same environment and differed only in feeding strategy. No statistically significant differences between fat content and muscle content were observed suggesting that balsam poplar-based supplements do not exert any effect on carcass composition.
According to organoleptic evaluation and external examination of carcasses and organs of the animals, no deviations were observed in appearance and colour of meat of all groups. Analysis of bacterioscopy of prints smears in meat revealed up to 2.6±0.51 mostly gram-positive coccal microorganisms in the surface of muscle tissue (Table 3). In the deep layers of the samples studied, no microorganisms were found.
The meat pH in all groups corresponded to meat obtained from healthy animals and was in the range from 5.8 ± 0.06 to 6.0 ± 0.05. No primary and secondary protein degradation products in the meat of all groups were observed. The benzidine reaction in all samples was positive.
Thus, major sensory as well as physical and chemical parameters were not negatively affected by balsam poplar-based supplementations (p > 0.05 for all).
In the recent years, plant-based bioactive supplements in animal diet received a growing attention as a means to increase production efficiency of ruminants. The phy-topreparations from balsam poplar buds contain amino acids, unsaturated fatty acids (malic, tartaric, lemon, lin-oleic, linolenic, arachidonic), polyphenols (pinostrobin, pinocerbrin, chrysin, tektohrizin, apigenin, kaempferol, quercetin, myricetin, galangin, iszalpinin etc.) carbohydrates, minerals (zinc, manganese, cobalt, copper, iron) and vitamins (A, C, P, E) [16,18,24,25]. Previous studies on the chemical composition of balsam poplar buds also identified the presence of alkanes, terpenes, chalcones and
prostaglandins [16,26,27]. This composition might lead to the improvement of fermentation in the rumen, improvement of metabolic processes and suppression of undesirable microflora of the rumen [28]. Additives based on bark from Populus tremuloides and leaves and stems from Populus tremula were shown have potential to decrease methane production during rumen fermentation [12, 29]. Additionally, many plant-based products represent potential alternative to treat various diseases due to the presence of bioactive compounds with therapeutic properties. Salicin, salicortin, salireposide, and populoside were also identified as bioactive components in balsam poplar responsible for the inhibition of adipogenesis and were suggested as complementary agents in antiobesity and antidiabetic therapies [30]. Currently, the use of the preparations from balsam poplar is limited. In Kazakhstan, balsam poplar buds are used to treat hemorrhoids and scurvy [31]. In Russia, fresh or dry balsam poplar buds are used in forms of an ointment or tincture and used in traditional medicine to treat rheumatism or cold [31]. Development of such preparations represents a complex effort demanding a highly integrated interdisciplinary approach. Recently, antioxidant, anti-inflammatory, hepatoprotective and vasorelaxant activities of Populus nigra flower buds ethanolic extract were evaluated using mice as an animal model [32]. It was shown that the extract had anti-inflammatory, hepatoprotective and vaso-relaxant properties and no toxic effect was observed after administration of 200 mg/kg during 4 weeks [32].
We believe that our results open new research avenues for the development of novel and safer additives for the improvement of livestock production.
Conclusion
Balsam poplar plants is well known as a rich source of bioactive compounds with positive health effects, and might be used in agriculture to support sustainable animal production and animal health without compromising meat quality and generating environmental impact issues. Animals from the groups fed 10% balsam poplar buds extract and dry shredded balsam poplar buds had significantly higher live weight compared to the control animals fed traditional diet without any supplement (P < 0.05). At slaughter, group fed 10% balsam poplar buds extract had significantly higher live weight compared to control.
Table 3. Sensory, physical and chemical examination of meat
Bacterioscopy Physical and chemical indicators
Group Boiling test Outer Inner Reaction with sulfuric acid copper* Reaction to Formaldehyde peroxidase* reaction* рн
1 clear broth 1.5 ± 0.25 0 - + ± 6.0 ± 0.07
2 clear broth 2.5 ± 0.25 0 - + ± 5.8 ± 0.02
3 clear broth 2.0 ± 0.0 0 - + ± 5.9 ± 0.04
4 clear broth 2.5 ± 0.25 0 - + ± 6.0 ± 0.05
5 clear broth 2.0 ± 0.5 0 - + ± 5.8 ± 0.04 * — Negative, + Positive, ± Doubtful
Average daily gain was also greatest in that group. Major sensory as well as physical and chemical parameters were not affected by balsam poplar-based supplementations (p > 0.05 for all) and were in line with regulatory meat hygiene requirements.
Our results indicated that the development of new balsam poplar-based additives for livestock can help to improve animal growth and performance. However, more research is required to establish the optimal dose and period of feeding.
REFERENCES
1. Duff, G. C. (2007). Integrating lifetime nutrition: from cow/ calf to stocker to feedlot. Veterinary Clinics of North America: Food Animal Practice, 23(2), 177-191, https://doi.org/10.1016/j. cvfa.2006.11.001
2. Hersom, M., Imler, A., Thrift, T., Yelich, J., Arthington, J. (2015). Comparison of feed additive technologies for preconditioning of weaned beef calves. Journal of Animal Science, 93(6), 3169-3178. https://doi.org/10.2527/jas.2014-8689
3. Larson, R. L. (2007). Heifer Development: Reproduction and Nutrition. Veterinary Clinics of North America: Food Animal Practice, 23(1), 53-68. https://doi.org/10.1016Zj.cvfa.2006.11.003
4. Puniya, A. K., Salem, A. Z. M., Kumar, S., Dagar, S. S., Griffith, G. W., Puniya, M. et al. (2015). Role of live microbial feed supplements with reference to anaerobic fungi in ruminant productivity: A review. Journal of Integrative Agriculture, 14(3), 550-560. https://doi.org/10.1016/S2095-3119(14)60837-6
5. Wegener, H. C. (2003). Antibiotics in animal feed and their role in resistance development. Curr Opin Microbiol, 6(5), 439445. https://doi.org/10.1016/j.mib.2003.09.009
6. Patel, S. J., Wellington, M., Shah, R. M., Ferreira, M. J. (2020). Antibiotic Stewardship in Food-producing Animals: Challenges, Progress, and Opportunities. Clinical therapeutics, 42(9), 16491658. https://doi.org/10.1016/j.clinthera.2020.07.004
7. Mennecke, B. E., Townsend, A. M., Hayes, D. J., Lonergan, S. M. (2007). A study of the factors that influence consumer attitudes toward beef products using the conjoint market analysis tool. Journal of Animal Science, 85(10), 2639-2659. https://doi. org/10.2527/jas.2006-495
8. Knicky, M., Sporndly, R. (2011). The ensiling capability of a mixture of sodium benzoate, potassium sorbate, and sodium nitrite. Journal of Dairy Science, 94(2), 824-831. https://doi. org/10.3168/jds.2010-3364
9. Castro, T., Cabezas, A., De la Fuente, J., Isabel, B., Manso, T., Jimeno, V. (2016). Animal performance and meat characteristics in steers reared in intensive conditions fed with different vegetable oils. Animal, 10(3), 520-530. https://doi.org/10.1017/ s1751731115002554
10. He, Z. X., He, M. L., Zhao, Y. L., Xu, L., Walker, N. D., Beauche-min, K. A. et al. (2015). Effect of wheat dried distillers grains and enzyme supplementation on growth rates, feed conversion ratio and beef fatty acid profile in feedlot steers. Animal, 9(10), 17401746. https://doi.org/10.1017/s1751731115000944
11. Hegarty, R. S., Miller, J., Oelbrandt, N., Li, L., Luijben, J. P., Robinson, D. L. et al. (2016). Feed intake, growth, and body and carcass attributes of feedlot steers supplemented with two levels of calcium nitrate or urea. Journal of Animal Science, 94(12), 5372-5381. https://doi.org/10.2527/jas.2015-0266
12. Rochfort, S., Parker, A. J., Dunshea, F. R. (2008). Plant bioac-tives for ruminant health and productivity. Phytochemistry, 69(2), 299-322. https://doi.org/10.1016/j.phytochem.2007.08.017
13. Benchaar, C., McAllister, T. A., Chouinard, P. Y. (2008). Digestion, ruminal fermentation, ciliate protozoal populations, and milk production from dairy cows fed cinnamaldehyde, quebracho condensed tannin, or Yucca schidigera saponin extracts. Journal of Dairy Science, 91(12), 4765-4777. https://doi.org/10.3168/ jds.2008-1338
14. Hashemzadeh-Cigari, F., Khorvash, M., Ghorbani, G. R., Kadi-var, M., Riasi, A., Zebeli, Q. (2014). Effects of supplementation with a phytobiotics-rich herbal mixture on performance, udder health, and metabolic status of Holstein cows with various levels of milk somatic cell counts. Journal of Dairy Science, 97(12), 7487-7497. https://doi.org/10.3168/jds.2014-7989
15. Valenzuela-Grijalva, N. V., Pinelli-Saavedra, A., Muhlia-Alma-zan, A., Domínguez-Díaz, D., González-Ríos, H. (2017). Dietary inclusion effects of phytochemicals as growth promoters in animal production. Journal of Animal Science and Technology, 59, 8-8. https://doi.org/10.1186/s40781-017-0133-9
16. Isidorov, V. A., Vinogorova, V. T. (2003). GC-MS analysis of compounds extracted from buds of Populus balsamifera and Populus nigra. Zeitschrift Fur Naturforschung — Section C Journal of Biosciences, 58(5-6), 355-360. https://doi.org/10.1515/znc-2003-5-612
17. Mattes, B. R., Clausen, T. P., Reichardt, P. B. (1987). Volatile constituents of balsam poplar: The phenol glycoside connection. Phytochemistry, 26(5), 1361-1366. https://doi.org/10.1016/ S0031-9422(00)81813-0
18. Piochon-Gauthier, M., Legault, J., Sylvestre, M., Pichette, A. (2014). The essential oil of Populus balsamifera buds: its chemical composition and cytotoxic activity. Natural product communications, 9(2), 257-260.
19. McWilliam, E. L., Barry, T. N., López-Villalobos, N. (2005). Organic matter digestibility of poplar (Populus) and willow (Salix) forage trees and its in vitro prediction. Journal of the Science of Food Agriculture, 85, 1098-1104.
20. Mellenberger, R. W., Satter, L. D., Millett, M. A., Baker, A. J. (1971). Digestion of aspen, alkali-treated aspen, and aspen bark by goats. Journal of Animal Science, 32(4), 756-763. https://doi. org/10.2527/jas1971.324756x
21. Simard, F., Gauthier, C., Legault, J., Lavoie, S., Mshvildadze, V., Pichette, A. (2016). Structure elucidation of anti-methicillin resistant Staphylococcus aureus (MRSA) flavonoids from balsam poplar buds. Bioorganic and medicinal chemistry, 24(18), 41884198. https://doi.org/10.1016/j.bmc.2016.07.009
22. National Research Council (US) Committee for the Update of the Guide for the Care and Use of Laboratory Animals. Guide for the Care and Use of Laboratory Animals. 8th edition. Washington (DC): National Academies Press (US); 2011. Retrieved from https://www.ncbi.nlm.nih.gov/books/NBK54050/ Accessed August 15, 2021 https://doi.org/10.17226/12910
23. Balji, Y., Adilbekov, Z., Scheiko, Y., Seidenova, S. P., Ismagu-lova, G., Zamaratskaia, G. (2018). A rapid and sensitive method to determine potassium permanganate in meat. Journal of Consumer Protection Food Safety, 14, 167-172. https://doi. org/10.1007/s00003-018-1202-9
24. Mamaeva, O. O., Isaeva, E. V. (2021). Use of Post-Extraction Fir Wood Greenery Residues by the Bioconversion Method with the Production of Feed Additives. Forests, 12(3), 272. https://doi. org/10.3390/f12030272
25. Movahedi, A., Almasi Zadeh Yaghuti, A., Wei, H., Rutland, P., Sun, W., Mousavi, M. et al. (2021). Plant secondary metabolites with an overview of populus. International journal of molecular sciences, 22(13), 6890. https://doi.org/10.3390/ijms22136890
26. Greenaway, W., May, J., Whatley, F. R. (1989). Flavonoid agly-cones identified by gas chromatography-mass spectrometry in bud exudate of populus balsamifera. Journal of Chromatography A, 472, 393-400. https://doi.org/10.1016/S0021-9673(00)94139-6
27. Levin, E. D., Isaeva, E. V., Cherepanova, V. E. (1990). Arachidon-ic acid and prostaglandins in buds of Populus balsamifera. Phyto-chemistry, 29(7), 2325-2326. https://doi.org/10.1016/0031-9422(90)83062-6
28. Khiaosa-ard, R., Zebeli, Q. (2013). Meta-analysis of the effects of essential oils and their bioactive compounds on rumen fermentation characteristics and feed efficiency in ruminants. Journal of Animal Science, 91(4), 1819-1830. https://doi.org/10.2527/ jas.2012-5691
29. Kuralkar, P., Kuralkar, S. V. (2021). Role of herbal products in animal production — An updated review. Journal of Ethno-pharmacology, 278, Article 114246. https://doi.org/10.1016/j. jep.2021.114246
30. Harbilas, D., Vallerand, D., Brault, A., Saleem, A., Arna-son, J. T., Musallam, L., Haddad, P. S. (2013). Populus bal-samifera extract and its active component salicortin reduce obesity and attenuate insulin resistance in a diet-induced obese mouse model. Evidence-based Complementary and Alternative Medicine, 2013, Article 172537. https://doi. org/10.1155/2013/172537
31. Shikov, A. N., Narkevich, I. A., Flisyuk, E. V., Luzhanin, V. G., Pozharitskaya, O. N. (2021). Medicinal plants from the 14th edition of the Russian Pharmacopoeia, recent updates. Journal of Ethno-pharmacology, 268, Article 113685. https://doi.org/10.1016/j. jep.2020.113685
32. Debbache-Benaida, N., Atmani-Kilani, D., Schini-Keirth, V. B., Djebbli, N., Atmani, D. (2013). Pharmacological potential of Populus nigra extract as antioxidant, anti-inflammatory, cardiovascular and hepatoprotective agent. Asian Pacific Journal of Tropical Biomedicine, 3(9), 697-704. https://doi.org/10.1016/S2221-1691(13)60141-0
AUTHOR INFORMATION
Yuriy Balji, Candidate of Veterinary Science, Associate Professor, Department of Veterinary Sanitary, S. Seifullin Kazakh Agro Technical University. 62, Zhenis avenue, 010011, Nur-Sultan, Republic of Kazakhstan. Tel.: +7-701-979-67-98, E-mail: [email protected] ORCID: https://orcid.org/0000-0002-5006-3224
Martin Knicky, PhD, Doctor of Philosophy, Researcher, Department of Agriculture and Food, RISE Research Institutes of Sweden, Box 7033, 750 07, Uppsala, Sweden. Tel.: +46-10-516-69-19, E-mail: [email protected] ORCID: https://orcid.org/0000-0001-5121-1957 * corresponding author
All authors bear responsibility for the work and presented data. All authors made an equal contribution to the work.
The authors were equally involved in writing the manuscript and bear the equal responsibility for plagiarism. The authors declare no conflict of interest.
