CC BY
19
Бюлог1чний вюник МДПУ iMeHi Богдана Хмельницького 6 (3) стор. 414—418, 2Ш6
Biological Bulletin of Bogdan Chmelnltskly Melitopol State Pedagogical University, 6 (3), pp. 4144—418, 2016
ARTICLE UDC582.232(477.64)
L.I. Arabadzhi1, A.M. Solonenkoi, O.G. Bren1,2, M.I. Holubev3 CYANOPROCARYOTA OF TUBALSKYI ESTUARY (AZOV SEA BASIN)
1Bogdan Chmelnitskiy Melitopol State Pedagogical University. Melitopol, Ukraine 2Prya%ov National Natural Park. Melitopol, Ukraine National University of Life and Environmental Sciences of Ukraine. Kiev, Ukraine E-mail: [email protected]
Information about species composition of Cyanoprocaryota representatives in the natural boundary of Tubalskyi Estuary within Pryazov National Natural Park is supplemented. Taxonomic structure of identified algae species is given, the data about occurrence of species in different habitats within national park is provided. We present a systematic structure and geographical affinity of discovered algae species. We registered 38 species of cyanoprocaryotic algae of orders: Chroococcales, Oscillatoriales and Nostocales with predominance of Oscillatoriales representatives in different habitats of Tubalskyi Estuary within the areas of Pryazov National Natural Park. The greatest number of algae species had genera Lyngbya, Phormidium, Microcoleus Leptolyngbya — 4 species per each genera. The majority of algae species were the cosmopolitans. Microcoleus tenerrimus was registered in all the studied plots of soil sampling and in the water reservoir of Tubalskyi Estuary. The most abundant and distributed species was Lyngbya aestuarii, which formed the macroscopic growths (cyanobacterial mats). Key words: Cyanoprocaryota, algae, Tubalskyi Estuary, Northern Ptya%pvye, national park.
Л.1. Арабаджи1, A.M. Солоненко1, О.Г. Брен1,2, M.I. Голубев3 Ц1АНОПРОКАРЮТИ УРОЧИЩА ТУБАЛЬСЬКИЙ ЛИМАН
1Мелтопольський державний mdazozi4nm утверситет iMeni Богдана Хмельницького 2Приазовський нащональний природний парк Нащональний утверситет бмреурав i природокористування Украти E-mail: [email protected]
Доповнен в^омоста видового складу представнишв в/ллму Cyanoprokaryota урочища Тубальський лиман в межах Приазовського нацюнального природного парку. Наводиться систематична структура виявлених вид^в водоростей, надано дат щодо трапляння виддв в рiзних бютопах урочища.
Ключгж слова: Cyanoprocaryota, водоростi, Тубальский лиман, Швтчне Приазов'я, нащональний парк.
Л.И. Арабаджи1, А.Н. Солоненко1, А.Г. Брен1,2, М.И. Голубев3 ЦИАНОПРОКАРИОТЫ УРОЧИЩА ТУБAЛЬСКИЙ ЛИМАН
1 Мелитопольский государственный педагогический университет имени Богдана Хмельницкого 2Приазовский национальный природный парк Национальный университет биоресурсов и природопользования Украины E-mail: [email protected]
Дополнены сведения видового состава представителей отдела Cyanoprokaryota урочища Тубальський лиман в пределах Приазовского национального природного парка. Приводится систематическая структура выявленных видов водорослей, предоставлены данные по встречаемости видов в различных биотопах урочища. Ключевые слова: Cyanoprocaryota, водоросли, Тубельский лиман, Северное Приазовье, национальный парк.
Citation:
Arabadzhi, L.I., Solonenko, A.M., Bren O.G., Holubev, M.I. (2016). Cyanoprocaryota of Tubalskyi Estuary (Azov Sea
basin). Biological Bulletin of Bogdan Chmelnitskiy Melitopol State Pedagogical University, 6 (3), 414—418.
Поступило в редакцию / Submitted: 18.11.2016
Принято к публикации / Accepted: 19.12.2016
eros http: / /dx.doi.org/10.15421 /2016112
© Arabadzhi, Solonenko, Bren, Holubev, 2016
Users are permitted to copy, use, distribute, transmit, and display the work publicly and to make and distribute derivative works, in any digital medium for any responsible purpose, subject to proper attribution of authorship.
This work is licensed under a Creative Commons Attribution 3.0. License
North-Western territories of the Azov Sea shore have unique landscapes and high biodiversity is extremely valuable in the economical aspect and have great recreational potential. In 2010 by decree of President of Ukraine the environmental institution — Pryazov National Natural Park (hereinafter - PNNP) was established for the rational use and conservation of natural resources in this area (Chronicles..., 2012). One of the main objectives of environmental institutions (including PNPP) is to carry out the monitoring researches of natural ecosystems. The cyanoprocaryotic algae could be used as the convenient and reliable bioindicators of processes in ecosystems. The results of researches of algae from the North-Western territories of the Azov Sea shore were presented in several articles (Solonenko et al., 2006, 2008), but cyanoprocaryotic algae within the natural boundary of Tubalskyi Estuary are studied unevenly and should be updated by additional data.
Natural boundary of Tubalskyi Estuary is situated near the Village Prymorskyi Posad. Its' territories have a flat landscape and formed by the mouth of River Domuzla (Fig. 1). The maximum length of the tract - 4.15 km, maximum width - 2 km, altitude of slopes of the right bank - 9 m. The coordinates of extreme points: North -46°37'5.93"N 35°42'11.27"E; South - 46°34'51.04"N 35°43'4.87"E; West - 46°36'18.83"N 35°40'49.32"E; East -46°35'46.97"N 35°43'58.63"E. Water regime depends mainly on precipitation as well as river water, sea water infiltration, periodic flooding through the sand bar. The maximum length of water - 300 m, maximum width - 100 m, maximum depth - 25 cm. The water surface area greatly varies and depends on the season. The salinity of the water is 55-112 %o; pH 6.1-6.4. Tubalskyi Estuary is included in the list of protected areas with a status of the wetland of regional importance.
The higher vegetation is presented here by plant communities with dominance of Phragmites australis (Cav.) Trin. ex Steud. and halophilic communities with dominance of Saicornia europea L., Halocnemum strobilaceum M. Bieb., Salsola soda L., Suaeda altissima (L.) Pall. There is a pond in the southern part of the natural boundary, and in the warmer months the Potamogetonpectinatus (L.) Börner develops powerful growths over there.
Figure 1. Map of sampling points
MATERIALS AND METHODS
The soil samples were carried out in the 2014-2015 on the four fixed sampling points in the natural boundary of Tubalskyi Estuary within the territory of PNNP (Pryazov district, Zaporizhzhya region), namely: steppe slopes, sandy soils, saline soils and in the reservoir. Samples were collected in spring, summer and autumn by the conventional phycology methods (Hollerbach, 1969; Topachevskyi, 1984). Totally, we sampled and processed 18 terrestrial samples and 9 water samples. The subsequent identification of algae was performed at the Department of Botany and Landscape Architecture in the laboratory of algal and ecological studies of the terrestrial and aquatic ecosystems in the Bogdan Chmelnitskiy Melitopol State Pedagogical University.
Collected material was processed using cultural methods: 1) soil culture with glasses of growth; 2) agaric Bold's nutrient media with normal and tripled quantity of nitrogen (1N BBM and 3N BBM, respectively) (Arce, 1958). Researches were held on by the light binocular microscope "MICROmed XS-5520" using 40x and 100x lenses. Identification was carried out by the identification guide of algae (Komarek, Anagnostidis, 1998; 2005). Analysis of the revealed algae species in relation to some geographical confinement was carried out according to Barinova (2006). Systematic list of the identified algae species was structured by system adopted in the monograph of I. Kostikov (2001) with additions and clarifications according to a check-list by Wasser (2000) and Tsarenko (2006).
RESULTS AND DISCUSSION
We identified 38 species of algae in the natural boundary of Tubalskyi Estuary. These species belongs to 3 orders, 12 families and 19 genera (Table. 1).
Table 1. Systematic structure of cyanoprocariotic algae, Tubalskyi Estuary
№ Taxon
Phylum Cyanoprocaryota
Class Cyanophyceae Sachs 1874
Order Chroococcales Cavalier-Smith, 2002
Family Merismopediaceae Elenkin 1933
Genus Synechocystis Sauvageau 1892
1. Synechocystis salina Wislouch 1924
2. Synechocystis crassa Woronichin 1929 Genus Merismopedia Meyen 1839
3. Merismopedia elegans A. Braun in Kützing 1849
4. Merismopedia glauca Kützing 1845
5. Merismopedia punctata Meyen 1839 Family Microcystaceae Elenkin 1933 Genus Microcystis Kützing 1907
6. Microcystispulverea (Wood) Forti emend Elenkin 1933 Genus Chondrocystis Lemmermann 1899
7. Chondrocystis sarcinoides Komârek et Anagnostidis 1995 Family Chroococcaceae Nägeli 1849
Genus Pseudocapsa Ercegovic 1925
8. Pseudocapsa sphaerica Kovâcik 1988 Family Entophysalidaceae Geitler 1925 Genus Johannesbaptistia De Toni 1934
9. Johannesbaptistiapellucida Taylor et Drouet 1938 Family Hyellaceae Borzi 1914
Genus Hyella Bornet et Flahault 1886
10. Hyella caespitosa Bornet et Flahault 1888 Order Oscillatoriales Elenkin 1934
Family Oscillatoriaceae Engler 1898
Genus Lyngbya Agardh 1892
11. Lyngbya aestuarii Liebman ex Gomont 1892
12. Lyngbya semiplena J. Agardh ex Gomont 1892
13. Lyngbya salina Kutzing ex Starmach 1966
BioAoziwuü eicHUK MAnY Mem Bozdama XMeMm^bKozo 6 (3), 2016
№ Taxon
14. Lyngbya major Meneghini 1892
Genus Oscillatoria Vaucher 1892
15. Oscillatoria tenuis Agardh ex Gomont 1892
16. Oscillatoria geminata Meneghini 1892
Family Phormidiaceae Anagnostidis et Komárek 1988
Genus Phormidium Kützing 1892
17. Phormidium uncinatum (Agardh) Gomont 1890
18. Phormidium autumnale Gomont 1892
19. Phormidiumpaulsenianum Boye-Petersen 1930
20. Phormidium takyricum Novitschkova 1960
Genus Pseudophormidium Anagnostidis et Komárek 1988
21. Pseudophormidium edaphicum (Elenkin) Anagnostidis et Komárek 1988
Genus Microcoleus Desmaziéres 1892
22. Microcoleus vaginatus Gomont 1892
23. Microcoleus paludosus (Kützing) Gomont 1892
24. Microcoleus tenerrimus Gomont 1892
25. Microcoleus chthonoplastes Thuret ex Gomont 1892
Genus Spirulina Turpin ex Gomont 1892
26. Spirulina subsalsa Oersted ex Gomont 1892
Family Plectonemataceae Elenkin 1934
Genus Plectonema Thuret 1892
27. Plectonema notatum (Schmidle) Anagnostidis & Komárek 1988
Genus Symploca Kützing 1892
28. Symploca elegans Kützing 1892
Family Schizotrichaceae Elenkin 1934
Genus Schizothrix Kützing 1892
29. Schizothrix coriacea Kützing ex Gomont 1892
30. Schizothrix friesii (Agardh) Gomont 1892
31. Schizothrix lardacea (Cesati) Gomont 1892
Family Pseudanabaenaceae Anagnostidis et Komárek 1988
Genus Lptolyngbya Anagnostidis et Komárek 1988
32. Lptolyngbya valderiana (Gomont) Anagnostidis et Komárek 1988
33. Lptolyngbya halophila (Hansgirg ex Gomont) Anagnostidis et Komárek 1988
34. Lptolyngbya boryana (Gomont) Anagnostidis et Komárek 1988
35. Lptolyngbya notata (Schmidle) Anagnostidis et Komárek 1988
Order Nostocales Cavalier-Smith 2002
Family Nostocaceae Eichler 1886
Genus Nostoc Vaucher 1886
36. Nostoc punctiforme (Kützing) Hariot 1891
37. Trichromus ellipsoporus (Fritsch) Komárek et Anagnostidis 1989
Family Rivulariaceae Kützing 1843
Genus Calothrix Agardh 1886
38. Calothrix parietina Thuret 1886
The greatest species diversity had order Oscilllatoriales (21 species) followed by Phormidiaceae — 10 species, Tyngbya, Phormidium, Microcoleus Teptolyngbya — 4 species. Such genera as Merismopedia, Schizothrix and Synechocystis. Oscillatoria were represented by 3 and 2 species respectively; other genera — by 1 species. According to literature data 19 identified algae species could be related to cosmopolitan (16) and Holarctic species (3). Among all studied habitats the largest number of cyanoprocaryotic algae (15 species) were found on the steppe slopes: Teptolyngbya boryana, Tyngbya semiplena, Microcoleuspaludosus, Microcoleus tenerrimus, Microcoleus vaginatus, Nostocpunctiforme, Oscillatoria
tenuis, Phormidium uncinatum, Pseudophormidium edaphicum, Schi%othrix coriacea, Schi%othrix friesii, Schi%othrix lardacea, Symploca elegans, Plectonema notatum, Trichromus ellipsosporus. Algae of the steppe slopes formed local greening-like macroscopic growths on the ground surface, which were observed in the lower parts of the slopes in the places of moisture accumulation. Also the great species variety was registered in saline soils (solonchak) and in the reservoir — 11 species per each habitat. In the salt marshes we founded such species as: Leptolyngbya halophila, Leptolyngbya valderiana, Lyngbya major, Lyngbya aestuarii, Lyngbya salina, Lyngbya semiplena, Microcoleus chthonoplastes, Microcoleus tenerrimus, Phormidium paulsenianum, Phormidium takyricum, Schi%othrix coriacea. Lyngbya aestuarii was the only species which formed the dark-green solid slime of macroscopic growths.
We identified Chondroystis sarcinoides, Hyella caespitosa, Johannesbaptistiapellucida, Merismopediapunctata, Microcystis pulverea, Microcoleus chtonoplastes, Microcoleus tenerrimus, Pseudocapsa sphaerica, Synechocystis crassa, Synechocystis salina, Spirulina subsalsa for the area of Tubalskyi Estuary whereas the characteristic algal water blooms were not observed in warm periods. The limited number of blue-green algae species were sampled in sandy soils: Calothrix parietina, Leptolyngbya notata, Lyngbya semiplena, Merismopedia elegans, Merismopedia glauca, Oscillatoria tenuis, Oscillatoria geminata, Phormidium autumnale where the algae did not form the massive growths during the research period. The most abundant and frequent species in all the habitats were Microcoleus tenerrimus, Microcoleus chthonoplastes, Lyngbya semiplena, Schi%othrix coriacea, while Microcoleus tenerrimus was registered in all studied habitats of terrestrial and water zone of Tubalskyi Estuary area. CONCLUSIONS
We registered 38 species of cyanoprocaryotic algae of orders: Chroococcales, Oscillatoriales and Nostocales with predominance of Oscillatoriales representatives in different habitats of Tubalskyi Estuary within the areas of Pryazov National Natural Park. The greatest number of algae species had genera Lyngbya, Phormidium, Microcoleus Leptolyngbya — 4 species per each genera. The majority of algae species were the cosmopolitans. Microcoleus tenerrimus was registered in all the studied plots of soil sampling and in the water reservoir of Tubalskyi Estuary. The most abundant and distributed species was Lyngbya aestuarii, which formed the macroscopic growths (cyanobacterial mats).
REFERENCES
Arce, G., Bold, H.C. (1958). Some Chlorophyceae from Cuban Soils. Amer. Bot. Journ, 45, 492-503.
Baraboha, N.M., Baraboha, A.P., Bren, O.G. (2012). Chronicles of Pryazov National Natural Park (2011). Pryazov National Park. Melitopol (in Russian).
Barinova, S.S., Medvedeva, L.A., Anisimova, O.V. (2006). Biodiversity of Environmental Indicator Algae. Tel Aviv: Pilies Studio.
Hollerbach, M.M., Shtina, E.S. (1969). Soil algae. Leningrad: Nauka (in Russian).
Komarek, J., Anagnostidis, K. (1999). Cyanoprokaryota. 1 Teil: Chroococcales. In:Ettl H., Gerloff J., Heynig H., Mollenhauer
D. (eds), Suswasserflora von Mitteleuropa, Gustav Fischer Verlag. Komarek, J., Anagnostidis, K. (2005). Cyanoprokaryota I. Oscillatoriales. Suswasserflora von Mitteleuropa. — Jena-Stuttgart-Lubeck-Ulm: Gustav Fischer.
Kostikov, I.Yu., Romanenko, P.A., Demchenko. E.M. (2001). Soil algae of Ukraine (history and methods, system synopsis of
flora). Kiev: Fitosotsiotsentr (in Russian). Solonenko, A.N., Yarovoy, S.A., Podorozhnyi, S.N., Raznopolov, O.N. (2006). Algae from the solonchak of the Stepanovska
and Fedotova Spits in the North-West coast of the Azov Sea. Soil Science, 7(3-4), 123-127. (in Russian). Solonenko, A.N., Yarovoy, S.A., Yarovaya, T.A. (2008). Algae from the solonchak of the mouth parts of river Korsak in the
natural boundary Tubalskyi estuary. Bulletin of the State Nikitsky Botanical Garden, 96, 26-29 (in Russian). Topachevskyi, A.V., Masuk, N.P. (1984). Freshwater algae of Ukrainian SSR. Kiev: Vyshaya Shkola (in Russian). Tsarenko, P.M., Wasser, S.P., Nevo, E. (2006). Algae of Ukraine. Diversity, Nomenclature, Taxonomy, Ecology and
Geography. Ruggell: A.R.A. Gantner Verlag K.G. Wasser, S.P., Tsarenko, P.M. (2000). Algae diversity of Ukraine. Phycology, 10(4), 1-309.
Yarovaya, T.A., Yarovoy, S.O., Bren, O.G. (2012). Algae of the Pryazov National Natural Park. Proceed. Int. Conf. The role of environmental institutions in preserving biodiversity, ethnic and cultural heritage and sustainable development areas. CBS: PNP Huzulschyna (in Ukrainian). Yarovoy, S.A., Yarovaya, T.A., Solonenko, A.N. (2008a). For the study of the solonchak algae on the Berdyansk Spit near the
lake Krasnoye. Ecology and Noospherology, 19(1-2), 160-162 (in Russian). Yarovoy, S.A., Solonenko, A.N., Yarovaya, T.A. (2008b). Macroscopic growths of algae on the sea shore solonchaks of the Azov Sea. Proceed. Int. Sc. Conf. and Marine Biology School "Modern problems of phycology". Rostov-on-Don (in Russian).
Yarovoy, S.A. (2012). Macroscopic growths of algae on the solonchaks of the Priazov National Natural Park (Ukraine).
Proceed. IV Int. Conf. Actual problems of modern phycology. Kiev (in Russian). Yarovoy, S.A., Solonenko, A.N., Olejnik, T.A. (2007). Soil algae of the sea shore solonchak of the Berdyansk Spit near the lake Krasnoye. Proceed. Int. Conf. Biology of the XXI century: theory, practice and teaching. Cherkasy—Kanev (in Russian).
Yarovyi, S.O., Yarovaya, T.A., Bren, O.G. (2013). Algological study of the Pryazov National Natural Park wetlands. In: Ecology of wetlands and peatlands. Kyiv: Dia (in Ukrainian).
Birnoziwuu eicHUK MAnY Mem Bozbama XMeabH^brnzo 6 (3), 2016
