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Protistology ■ 21
infer the morphology of the tintinnid ancestor's morphology. Especially, the ventral organelles of the Tintinnidiidae require a closer inspection owing to their uncommon de-novo-origin which contrasts the intrakinetal proliferation of basal bodies in the other somatic kineties of the Tintinnidiidae and the somatic ciliature in all other tintinnids. Accordingly, the morphology of lorica and cell of T. mucicola are described based on live observations and protargol-impregnated material and the cell division pattern is analysed with special emphasis on the development of the ventral organelles. The study indicates that all somatic ciliary rows of T. mucicola are exclusively dikinetidal and develop by intrakinetal proliferation of basal bodies and thus demonstrate the plesiomorphic character states already present in the dorsal rows of the euplotids and hypotrichs as well as in the girdle and ventral kineties of the Oligotrichida. The species has two obliquely orientated kineties resembling the ventral organelles in the other Tintinnidium species and the monotypic genus Membranicola. Hence, the ancestor of the tintinnids apparently had exclusively dikinetidal somatic kineties all reproducing by intrakinetal proliferation of basal bodies, and the de-novo-origin of the ventral organelles in the remaining Tintinnidiidae necessitates confirmation. The study was financially supported by the Austrian Science Fund (FWF): Project P28790.
THE BLACK SEA TINTINNIDS (SPIROTRI-CHEA, CHOREOTRICHIA, TINTINNIDA): CURRENT STATE OF BIODIVERSITY AND SPECIES COMPOSITION Gavrilova N.A.
Institute of Marine Biological Researches, Russian Academy ofScience, Plankton department, Laboratory ofmicroplankton [email protected]
According to literary and own data considering recent nomenclature revision there were 20 tintinnid choreotrich species in the Black Sea: Codonella lagenula, Helicostomella subulata, Tintinnopsis campanula, T. compressa, T. lobiancoi, T. rossolimi, T. baltica, T. karajacensis, T. cylindrica, T. tubulosa, T. beroidea, T. meunieri, T. urnula, T. minuta, Tintinnidium mucicola, Metacylis jorgensenii, M. mereschkovskii, Favella ehrenbergii, Stenosemella ventricosa and S. nivalis.
It should be noted that against the background of stable level of tintinnid diversity we observed the significant changes in the species composition ofthe group. Thus from 2001 to 2015 we registered 8 invasive species: Eutintinnus lususundae, E. tubulosus, E. apertus, Eutintinnus sp., Salpingella decurtata,
Tintinnopsis tocantinensis, Rhizodomus tagatzi and Amphorellopsis acuta replacing the indigenous species as Codonella lagenula, Helicostomella subulata, T. compressa, T. lobiancoi, T. rossolimi and Metacylis jorgensenii. However, the indigenous Tintinnopsis campanula, which was not observed during 10 years (since 1999) again, began to be present in the plankton from 2009 constantly. The finds of alien species are not rare, during certain seasons they reach high abundances. For example, such species as Eutintinnus lususundae and E. tubulosa are often dominating in the plankton community.
The reasons for mentioned changes are not known exactly. It is possible that these related with invasion of ctenophore Mnemiopsis leidyi into the Black Sea, which caused significant changes in the plankton community structure. Resulting the current list of the Black Sea Tintinnida consist of 23 species including invasive.
MITOCHONDRIAL TRANSCRIPTOME OF LEPTOMONAS PYRRHOCORIS H10 - CLOSE MONOXENOUS RELATIVE OF LEISHMANIA CLADE
Gerasimov E.S.1, Flegontov P.N.2, Gasparyan A.V.1, Logacheva M.D.3, Kolesnikov A.A.1
1 - DepartmentofBiology, M.V. LomonosovMoscow State University, 119991, Moscow, Russia
2 - Biology Centre, Institute of Parasitology, Czech Academy of Sciences, 370 05 Ceske Budejovice (Budweis), Czech Republic
3 - Beloz.ersky Institute of Physico-ChemicalBiology, M.V. Lomonosov Moscow State University, 119991, Moscow, Russia
Mitochondrial genome of trypanosomatids have several unusual traits both in it's structural organization and in it's expression. About 22 mitochondrial genes are compactly grouped in coding region of maxicircle - major coding component of the mt-genome. Some transcripts undergo extensive RNA-editing process before they become mature mRNAs and this is mediated by small gRNAs coded in minicircles. Biological advantages of such complex expression system remains unclear but it definitely plays crucial role in mitochondrial genome expression. Editing is well-studied in some dixenous species like Trypanosoma brucei and Leishmania tarentolae but less data exists for monoxenous species. In this work we present a full mitochondrial transcriptome map of Leptomonas pyrrhocoris H10 generated using RNAseq data. The overall transcription profile appeared to be similar to profile of Leishmania tarentolae.
