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COPAIFERA LANGSDORFFII DESF. KUNTZE (LEGUMINOSAE) PROMOTES SKIN WOUND HEALING IN RATS THROUGH DECREASE OF WOUND AREA AND EXTRACELLULAR MATRIX REMODELING
© Gushiken L.F.S.1, Hussni C.A.2, Rozza A.L.1, Lemos M.3, Polizello Junior M.3, Mangabeira da Silva J.J.3, Bastos J.K.3, Pellizzon C.H.1
11nstitute of Biosciences of Botucatu, Department of Morphology/IBB/UNESP, Botucatu, Sao Paulo, Brazil;
2 School of Veterinary Medicine and Animal Science, Department of Surgery and Veterinary Anesthesiology/FMVZ/UNESP, Botucatu, Sao Paulo, Brazil;
3 School of Pharmaceutical Sciences of Ribeirao Preto, Department of Pharmaceutical Science/FCFRP/USP, Ribeirao Preto, Sao Paulo, Brazil
When occurs an injury in the skin, the organism starts the wound healing, a physiological process that can be didactically divided into 3 phases: inflammatory, proliferative and remodeling. Non-healing wounds have a significant impact in public health and in the expenditure of public resources, because the wounds can cause physical and psychological deficiency or even death. Due to the high costs in maintenance and impact in public health, many studies have been made about skin wound healing, exploring new therapeutic strategies to accelerate wound closure, including the use of plants and natural products. Due to reports of popular knowledge using Copaifera langsdorffii Desf. Kuntze (Leguminosae) in skin wound healing, we analyzed the healing potential of hydroalcoholic extract of the leaves (EH) and oleoresin of the bark (OR) of Copaifera langsdorffii in rat skin wounds and the mechanisms involved in the process. We divided male Wistar rats in groups (n = 8) Lanette, Collagenase,
10% EH or 10% OR. After anesthesia, one wound of 2 cm was made in the back of animals. The wounds were treated once a day during 3, 7 or 14 days and the wound areas were measured. The rats were euthanized and skin samples destined to immunohistochemical analysis of collagen 1, collagen 3, FGF-2 and TGF-pi. All data were analyzed by ANOVA followed by Newman-Keuls, with p < 0.05. Our results showed a macroscopic retraction of the wounds of 10% EH and 10% OR creams compared with Lanette. Immunohistochemical results showed the activity of 10% EH and 10% OR in fibroblast proliferation and extracellular matrix remodeling, with increase of collagen 1, FGF-2 and TGF-P1 and decrease of collagen 3 after 14 days. Therefore, we demonstrated the potential of the treatments 10% EH and 10% OR in skin wound healing through the decrease of wound area and remodeling of extracellular matrix after 14 days of treatment. Financial support by Sao Paulo Research Foundation - FAPESP.
SEPARATION AND IDENTIFICATION OF DIARYLHEPTANOIDS FROM MORUS ALBA L.
© Rita Pénzes, Annamâria Mészâros, Anita Tôth, Andrâs Darcsi, Szabolcs Béni, Âgnes Alberti
Semmelweis University, Faculty of Pharmacy, Department of Pharmacognosy, Budapest
Diarylheptanoids are natural products, composed of two aromatic rings joined by a heptane chain. These phenolic compounds, characteristic for Alnus, Betulaand Curcuma species (0-2), are in the focus of interest due to their various pharmacological effects: anti-cancer and anti-inflammatory activities. Diarylheptanoids, however, have not been described in the Morus genus. M. alba L. (white mulberry) is a tree or shrub native to China and widely cultivated in Europe, Southeast Asia, and also in the United States. Previous phytochemical studies on M. alba reported polyphenols (flavonoids, anthocyanins,
chalcones stilbenoids, coumarins), organic acids, terpenoids and alkaloids.
The aim of our work was the phytochemical screening of diarylheptanoid composition in M. alba, as well as the separation, purification and structural characterization of the main diarylheptanoid compounds.
Extraction of M. alba bark samples was carried out with suitable solvents for diarylheptanoids. For the analysis of the extracts high-performance liquid chromatography equipped with a diode-array detector and coupled to tandem mass spectrometry with an
Obzory po kliniceskoj farmacologii i lekarstvennoj terapii [Reviews of clinical pharmacology and drug therapy] vol. 15/2017/suppLement 1
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electrospray ion source (HPLC-DAD-ESI-MS/MS) was applied.
The presence of diarylheptanoids in the bark of M. alba was proven for the first time. The analytes of interest were isolated using column chromatography preparative thin-layer chromatography, and preparative HPLC and they were identified by LC-MS/MS and nuclear magnetic resonance (NMR) spectroscopy. The major diarylheptanoids were oregonin and hirsutenone. Further diarylheptanoids, platyphyllonol derivatives,
hirsutanonol and its various glycosides as minor compounds were also detected. The evaluation of in vitro antioxidant and cytotoxic activity of the isolated compounds is in progress.
References:
1. Haining L, Gaimei S. 2012. Rec. Nat. Prod. 6:321-333.
2. Liu D, Liu YW, Guan FQ, Liang JY. 2014. Fitoterapia. 96:76-80.
3. Chan EW, Lye PY, Wong SK. 2016. Chin. J. Nat. Med. 14: 17-30.
LUPEOL, A TRITERPENE ISOLATED OF BOWDICHIA VIRGILIOIDES KUNTH. (STEAM BARK) ACCELERATES CUTANEOUS WOUND HEALING IN DIABETIC RATS
© Fernando Pereira Beserra1, Ana Júlia Vieira1, Eduardo Oliveira de Souza1, Lucas Fernando Sérgio Gushiken1, Carlos Alberto Hussni2, Gabriela Lemos de Azevedo Maia3, Ariane Leite Rozza1, Cláudia Helena Pellizzon1
1 Department of Morphology, Institute of Biosciences, Sao Paulo State University, Botucatu, Sao Paulo, Brazil;
2 Department of Surgery and Anaesthesiology of Large Animals, Faculty of Veterinary Medicine and Animal Science, Sao Paulo State University, Botucatu, Sao Paulo, Brazil;
3 Department of Pharmacy, Federal University of Sao Francisco Valley, Petrolina, Pernambuco, Brazil
Diabetes mellitus is a multisystem disorder and its complications induce physiological changes in tissues and cells that delay the normal healing process. It's known that prolonged inflammation and increased oxidative stress impairs wound healing in diabetics. Thus, considering that Lupeol, a well known anti-diabetic, anti-microbial, antioxidant, and anti-inflammatory agent, may be an important strategy in improving impaired healing in diabetics, the aim of this study was to evaluate the cutaneous wound healing potential of topically applied lupeol in diabetic rats. Diabetes was induced in male Wistar rats by a single intraperitoneal injection of streptozotocin (55 mg/Kg) 7 days before induction of lesion. After the diabetic state of the animals has been proven, a full-thickness excisional lesion (2 cm2) was created. These animals were treated with lanette ointment, insulin ointment (0.5 U/g) and lupeol ointment (0.2%, w/w). The wound area was measured daily and the animals were killed 14 days after the treatment. Quantification of Tumor
Necrosis Factor alpha (TNF-a), Interleukin-1 beta (IL-1P), Interleukin-6 (IL-6) and Interleukin-10 (IL-10) levels were performed on skin samples by ELISA method. Results were expressed as the mean ± standard error of the mean (S.E.M.). Statistical comparisons were performed by one-way analysis of variance (ANOVA) followed by Newman-Keuls test. The wound area was significantly smaller in the lupeol- and insulin-treated group than in the lanette group 14 days after lesion. Topical treatment with lupeol ointment was shown a reduction in the inflammatory response through decreasing the levels IL-6 and a significant increase in IL-10 levels. No alterations of TNF-a and IL-1 p levels were observed. Therefore, these data indicate clearly anti-inflammatory potential of lupeol topical treatment by modulating pro- and anti-inflammatory cytokines in the wound healing process and so may become an additional novel therapeutic agent in the management of impaired wound healing in diabetics. Financial support: Fapesp: 2014/23247-4
Obzory po kliniceskoj farmacologii i lekarstvennoj terapii [Reviews of clinical pharmacology and drug therapy]
vol. 15/2Q17/suppLeMEnT 1
