Научная статья на тему 'In vitro wound healing potential of the triterpene lupeol isolated from the steam bark of bowdichia virgilioides Kunth'

In vitro wound healing potential of the triterpene lupeol isolated from the steam bark of bowdichia virgilioides Kunth Текст научной статьи по специальности «Фундаментальная медицина»

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Текст научной работы на тему «In vitro wound healing potential of the triterpene lupeol isolated from the steam bark of bowdichia virgilioides Kunth»

Abstracts. PHYTOPHARM 2017

IN VITRO WOUND HEALING POTENTIAL OF THE TRITERPENE LUPEOL ISOLATED FROM THE STEAM BARK OF BOWDICHIA VIRGILIOIDES KUNTH

© Fernando Pereira Beserra1, Gabriela Lemos de Azevedo Maia2, Ariane Leite Rozza1, Claudia Helena Pellizzon1, Meilang Xue3, Christopher Jackson3

1 Department of Morphology, Institute of Biosciences, Sao Paulo State University, Botucatu, Sao Paulo, Brazil;

2 Department of Pharmacy, Federal University of Sao Francisco Valley, Petrolina, Pernambuco, Brazil;

3 Sutton Arthritis Research Laboratories, Kolling Institute of Medical Research, University of Sydney, Sydney, Australia

Keratinocytes are a major cell type of human skin and play a fundamental role in normal skin metabolism and cutaneous wound healing. Many natural products and medicinal plants have been used for study in vitro in the wound healing. For that reason, the aim of this study was to evaluate the effect of Lupeol (LU) on cell migration and wound healing using the scratching model as well as to determine its signaling mechanisms. Human primary epidermal keratinocytes were obtained from human neonatal foreskin in accordance to the guidelines of the local ethics committee. Cells were seeded into 24-well plates and cultured to subconfluence for in vitro migration and wound healing "scratch" assay treated with LU in different concentrations at 0.1, 1, 10 or 20 ^g/mL. Keratinocytes were lysed with lysis buffer, supplemented with protease inhibitor and phosphate inhibitor for western blot analysis. The primary antibodies used were: Akt, phospho-Akt, Erk, phospho-Erk, Tie-2, phospho-Tie-2, NF-kBp65 and MMP-2. Results were expressed as the mean ± standard error of the mean (S.E.M.). Statistical comparisons were performed by one-way

analysis of variance (ANOVA) followed by Tukey test. P< 0.05 was considered significant. In the in vitro test performed to evaluate wound healing, LU accelerated wound closure at all concentrations used in the study, whereas in the cellular migration assay, only the two lowest concentrations (0.1 and 1 ^g/mL) stimulated cell migration significantly in relation to the control. LU also showed increase in the protein expression of Akt, phospho-Akt, Tie-2, phospho-Tie-2 and MMP-2 and a decrease in the NF-kB expression was observed. These results demonstrate a central role for LU in promoting cell migration through activating Akt and its phosphorylated form (p-Akt), Tie-2 and phospho-Tie-2, reduction of inflammation by inhibition of NF-kB, and increasing of MMP-2 expression. These regulatory properties implicate lupeol as having potential to promote re-epithelialization during wound healing and remains a novel and exciting avenue of investigation.

Financial support: CsF/CNPq: 202513/2015-71, Fapesp: 2014/23247-4 and SuttonLab

INVESTIGATION OF THE INTERACTIONS BETWEEN HUMAN GUT BACTERIA AND HERBAL MEDICINAL PRODUCTS IN VITRO USING A COMBINED LC-HRMS- AND 16S RNA SEQUENCING APPROACH

© Eva-Maria Pferschy-Wenzig13, Kaisa Koskinen2,3, Alexandra Rossmann2, Karin Ardjomand-Woelkart1, Christine Moissl-Eichinger23, Rudolf Bauer1,3

11nstitute of Pharmaceutical Sciences, Department of Pharmacognosy, University of Graz; Graz, Austria;

2 Department of Internal Medicine, Medical University Graz; Graz, Austria;

3 BioTechMed, Graz, Austria

The microbial community colonizing the distal gut does not only have a strong impact on its human host's health, but also an enormous metabolic capacity. Compounds orally ingested via food or medicines that remain unabsorbed in the upper gastrointestinal tract are potentially degraded by gut microbes. This can lead to the formation of potentially bioavailable and bioactive metabolites. This aspect was often A platform should be established for studying the interaction between herbal medicinal product constituents and human gut bacteria. Several approved herbal medicinal products should be

tested, including willow bark extract, hawthorn leaves and flowers extract, and a fixed combination consisting of nine herbal extracts.

Extracts were incubated with 10% human fecal suspension under physiological conditions for 24 h. Samples were taken at 0.5 h, 4 h and 24 h. Changes in the composition of plant extracts were monitored by LC-HRMS analysis. Microbiome shifts were tracked by means of 16S RNA sequencing.

LC-HRMS analysis allowed to detect the microbial degradation of many compound classes occurring in

Obzory po kliniceskoj farmacologii i lekarstvennoj terapii [Reviews of clinical pharmacology and drug therapy] vol. 15/2017/suppLement 1

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