CC BY
24Физика живого, Т. 21, No1-2, 2014. С. 43-45. © Bogatina N.I., Sheykina N.V.
УДК577.3
ROLE OF HYDRONIUM IONS IN BIOLOGICAL EFFECTS OF WEAK COMBINED
MAGNETIC FIELDS
bogatina N.I., 2Sheykina N.V.
1 B. Verkin Institute for Low Temperature Physics&Engineering of NAS of Ukraine, Kharkov, Ukraine
e-mail: n_bogatina@rambler.ru 2 National University of Pharmacy, Kharkov, Ukraine e-mail: sheykina@ukr.net
Надшшла до редакци 10.09.2014
It was revealed that effects of low level of magnetic field's noise (less than 4^.T/Hz°'5) on gravitropic reaction of cress-roots depend on MF frequency. The peak of effects corresponded to cyclotron frequency of Ca2+ ions for the static component of combined magnetic field equal to 40^T. This peak split up into three frequency modes ( f1 = 31.3Hz, f2 = 32.5Hz and f3 = 34 Hz). The frequency f1 corresponds to Ca2+ ion (the theoretical value is 31.6 Hz), the frequency f2 corresponds to hydronium ion H3O+ (the theoretical value is 32.9 Hz), and frequency f3 relates OH- ion (the theoretical value is 35 Hz).Taking into account the influence of combined magnetic field on the hydronium ions and the Del Giudice hypothesis the main doubts about the existence of ion cyclotron resonance may be discard. Hydronium ions are unusual because they have a long free path. It was revealed that the combined magnetic field tuned for the cyclotron frequency of hydronium ion changed pH of pure water. Such changes have to lead to biological effects.
Key words: gravitropic reaction, combined electromagnetic field, cyclotron frequency, hydronium ion.
INTRODUCTION
Many hypotheses [1-4] were proposed after Liboff's discovery that combined magnetic field influenced on biological objects through ion cyclotron resonance. But all these hypotheses had some deficients. They couldn't explain the big free path of ion in the liquid. It is well known that the main liquid in the alive system is the water. Liboff's idea cannot explain long free path in liquid. It is well known, that the main liquid in alive systems is the water. Besides it has been proved until now that the water in biological system consisted from two phases. The first one was the usual water, the second one was the EZ water. This was the water in which it was ordered [5, 6]. Taking into account the influence of combined magnetic field on hydronium ions (H30) and also Del Giudice' hypothesis may throw away doubts about the possibility of ion cyclotron resonance. The hydronium ions are unusual because they have a long free path length.
MATERIAS AND METHODS
In erlier works we noticed that well reproducible magnetic conditions is the key factor in magnetobiolocal researches. . The method of obtaining such conditions was described by us in details [9-13]. In most of these works the many layers ^-metal shields were used. The static or combined magnetic field was created inside of them by means of coaxial
solenoids. It was shown, that in spite of good decreasing of static component of the Earth's magnetic field (until to 1000 times), the artificial creation of magnetic field inside the ^-metal shield led to the increasing of magnetic noise's level connected with current flowing in solenoids.
1
■0+fiii ;
Fig.1. 1 - volume for liquid helium, 2 - external cryostat for helium ., 3 -isolating cryostat , 4 - superconductive magnetic shield , 5 - Warm work volume, 6 - upper flange, 7 - the tube for flooding of liquid helium, 8 -the tube for flooding of liquid nitrogen, 9 - nitrogen screens of isolating (3)and external helium cryostats.
Bogatina N.I., Sheykina N.V.
À 1 là
ÏÇjî 3 l( 1]((
F- = r| I. irr:1/ II?
Fig.2. The dependence of magnetic noise's spectral density on frequency inside the warm volume of superconductive shield (the leaden shield was used) on the frequency. The measurements were fulfilled by means of SQUID -magnetometer. The curve corresponded to the remained magnetic field 5 nT when the field has been pushed out at most.
It is possible to decrease the level of magnetic fields essentially only by using the superconductive magnetic shield with warm volume (fig.1). We may freeze the magnetic field from until 43^T in this shield. The amplitude of magnetic field spectral density of such a shield is shown on fig.2. As can be seen from fig.2 we can decrease the magnetic field noise level down to 0.2 nT/Hz0 5 at the frequency 50 Hz for bold leaden shield. This is approximately 100 times less than for ^-metal shield. The 1/f region moved down to 0.01 Hz instead of 0.5 Hz for ^-metal shield.
It is well seen from the fig.2 that both the frequencies of building oscillation (5.6 and 9.5 Hz), and Shuman's frequencies (7..8 , 12.5 and h 23 Hz and frequencies 50, 100 h 150 Hz were presented.
Two-days germinated seeds were located in the remained field. Static and alternative components of artificial magnetic field was created by means of solenoids, located inside the superconductive shield. The seeds were germinated so that the roots were straight without curvatures [9 - 13].
The roots were located parallel to the Earth that is perpendicular to the gravitation force and the direction of combined magnetic field. The roots were located in wet thermo stable chamber. The humidity was 100%, the temperature change was not more than 0.2o during the day.
RESULTS AND DISCUSSION
2+
The frequency f1 corresponded to the Ca ion (theoretical value 31.6 Hz), the frequency f2 corresponded to the hydronium ion H3O+ (theoretical value 32.9 Hz), the frequency f3 corresponded to OH ion (theoretical value 35 Hz) (Fig.3).
f, Hz
Fig. 1. The dependence of the part of the roots with anomalous gravitropic reaction on frequency near the cyclotron frequency of calcium ions.
On fig.4 the graph of dependence of pH on the time of exposition in combined magnetic field tuned for hydronium ions is shown on fig.4. As one can see from the fig.4 after approximately 5 minutes after the combined magnetic field action pH of pure water decreased from 7 down to 6. The effect was observed in 75% of cases.
PH
M
-F—1-r | I ■■,"■( V I -r-,-
100 200 300 400 500 600
time, sec
Fig.4. Dependence of pH on time of exposition in combined magnetic field tuned for hydronium ions
Direct participation of hydroxonium ions in gravitropic reaction may explain non simple results obtained by us before . The observation of gravitropic reaction by different methods (during growing on vertical plane and in the wet air) the water ions had different direction of moving during they absorbed. This fact may lead to different biological effects [4,14]. And the hydronium ions moving may have different directions in EZ zone and depends both on the direction of hydronium ions moving and on the initial root's direction.
The decreasing of water pH indicates on the increasing of quantity of protons in pure water.
CONCLUSIONS
The peak in frequency depended MF-efects on roots gravitropic reaction corresponded to the cyclotron frequency of hydronium ions.
60
50-
40-
10
0
10
24
28
30
34
36
38
42
ROLE OF HYDRONIUM IONS IN BIOLOGICAL EFFEC TS OF WEAK CO MBINED MAGNETIC FIELDS
The decrease of pH in pure water was revealed
during exposure this water to combined magnetic field
with alternative component that tuned to the cyclotron
frequency of hydronium ions.
References
1. Liboff A.R., Parkinson W.C. Search for ion-cyclotron resonance in an Na+- transport system// Bioelectromagnetics. - 1991. - 12. - №2. - P. 78-84
2. Lednev V.V. Bioeffects of weak combined static and alternative magnetic fields // Biophysics. - 1996- ' Vol. 41. - №.1. - P. 224-232.
3. Bingi V.N., Savin A.V. Physical problems of weak magnetic fields action on biolo gical sy stems // Advances in Physical Sciences. - 2003. - Vol. 173, №3. - P. 265-300.
4. Bogatina N.I., Sheykina N.V., Kordyum E.L. Maize and cress roots orientation in combined magnetic field change roots gravitropic reaction response on its action. Experiment and new hypothesis . Adaptation mechanism //Scientifi c Notes of Taurida V. I. Vernadsky National University. - Series: «Biology, chemistry». -, 2012. -Vol.25. - №2. - P. 16-31.
5. Pollak G.H. The forth phase of water. -.Seattle: Ebner, 250 p.
6. Pollak G.H., Clegg J. Unexpected linkage between unstirred layers, exclusion zones and water. In Phase Transitions in Cell Biology; Ed.Pollak G.H., Chin W.C. Springer Science & Business Media: Berlin, Germany, 2008. - P.143-152.
7. Del Giudice E., Fleishmann V., Preparata G., Talpo J. On the "unreasonable" effects of ELF magnetic fields
upon a system of ions // Bioelectromagnetics. - 2002. -Vol. 23. - P.522-530.
8. Del Giudice E. EZ-water in the light of quantum field theory // Conference on the physics, chemistry and biology of water. Borovets, Bulgaria, 2013, Oct. 22 - 25.
9. Bogatina N.I., Sheykina N.V. Intrinsic magnetic field of wheat seed s and magnetic noises generataed by them / // Biophysical bulletin .- 2001.-Vol.525.- № 1(8). -P.101 - 107.
10. Bogatina N.I., Sheykina N.V., Kordyum E.L Karachevtsev V.A. Gravitropic reaction of plants in combined (static and alternative) magnetic field. // Reports ofNational Academy of Sciences of Ukraine. -2002. - №4. -P. 176 - 179.
11. Bogatina N.I., Sheykina N.V., Kordyum E.L Influence of combined magnetic field on gravitropic reaction of plants and spectrum of electromagnetic irradiation generated by them //Radiophysics and Electronics. -2005. - Vol. 10, №2. - P.331 - 335.
12. KordyumE.L., BogatinaN.I., KalininaYa. M., Sheykina N.V. A weak combined magnetic field changes root gravitropism // Advances in Space Research. - 2005. -Vol. 36. - №7. - P. 1229 - 1236.
13. Bogatina N.I., Sheykina N.V., Kordyum E.L. Dependence of gravitropic reaction of cress roots on amplitude of alternative component of combined magnetic field // Biophysical bulletin. - 2007 - №18(1) -P. 78 - 90.
14. Bogatina N.I. , Sheykina N.V. Influence of different relative orientation of ststic and alternative magnetic fields and cress roots on their gravitropic reaction Scientific Notes of Taurida V. I. Vernadsky National University. - Series: «Biology, chemistry». - 2014. -Vol.27. - №1. - P.24-28.
РОЛЬ 1ОН1В ГВДРОКСОНИЯ У БЮЛОГМНИХ ЕФЕКТАХ СЛАБКИХ КОМБ1НОВАНИХ МАГН1ТНИХ ПОЛ1В
Богатша Н.1., Шейкша Н.В.
Було виявлено, що вплив слабких шумових магнпних полш (менше 4[хТ / №и'5) впливають на грав^отчну реакцю крес-корешв в залежноси вщ частоти МП. Пж впливу вщповщала циклотронного частоти юшв Са2 + для статичного компонента комбшованого магнггного поля, рiвноl 40^Т. Цей пж розщеплюеться на три частотних смуги (А = 31.3 Гц, F2 = 32.5№ i f3 = 34 Гц). Частота А вщповщае юну Са2 + (теоретичне значення 31,6 Гц), частота 12 вщповщае юну гщроксошю Н3О + (теоретичне значення 32,9 Гц) i частота 13 вщноситься до юну ОН- (теоретичне значення 35 Гц) . 1они гидроксония незвичайт, тому що вони мають тривалий вшьний пробну. Було виявлено, що в магните поле, що налаштована на циклотроны частоти юна пдроксошю змшюеться рН чисто! води. Таю змши рН повинш приводити до певних ефекпв бюлоичного впливуМП.
Ключов1 слова: грав^ошчна реакщя, комбшоване електромагнггне поле, циклотронна частота, юн гщроксошю.
РОЛЬ ИОНОВ ГИДРОКСОНИЯ В БИОЛОГИЧЕСКИХ ЭФФЕКТАХ СЛАБЫХ КОМБИНИРОВАННЫХ МАГНИТНЫХ ПОЛЕЙ
Богатина Н.И., Шейкина Н.В.
Было выявлено, что влияние слабых шумовых магнитных полей (менее 4[хТ / №"'5) влияют на гравитропичную реакцию кресс-корней в зависимости от частоты МП. Пик влияния отвечал циклотронной частоте ионов Са2+ для статического компонента комбинированного магнитного поля, равной 40[хТ. Этот пик расщепляется на три частотных полосы (А = 31.3 Гц, F2 = 32.5Гц и 13 = 34 Гц). Частота А соответствует иону Са2+ (теоретическое значение 31,6 Гц), частота 12 соответствует иону гидроксония Н3О+ (теоретическое значение 32,9 Гц) и частота 13 относится к иону ОН-(теоретическое значение 35 Гц). Ионы гидроксония необычные, потому что они имеют длительный свободный пробега. Было обнаружено, что в магнитное поле, настроенном на циклотронную частоту иона гидроксония, меняется рН чистой воды. Такие изменения рН должны привоодить к определенным биологическим еффектам воздействия МП.
Ключевые слова: гравитропическая реакция, комбинированное электромагнитное поле, циклотронная частота, ион гидроксония.
