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Compound К UV (nm) ESI-MS+ m/z Ion detected
Catalpol 1,20 200 385,11 [M+Na]+
Deacetylasperulosidic acid 1,41 235 413,10 [M+Na]+
Aucubin 1,75 200 369,12 [M+Na]+
Asperulosidic acid 2,87 235 455,11 [M+Na]+
Harpagide 2,37 200 387,13 [M+Na]+
Leonuride 2,43 200 371,13 [M+Na]+
Galiridoside 2,57 200 369,12 [M+Na]+
Loganin 3,45 235 413,15 [M+Na]+
Acetylharpagide 3,50 200 429,14 [M+Na]+
Ajugoside 3,67 200 413,14 [M+Na]+
Oleuropein 4,29 235 563,19 [M+Na]+
Harpagoside 5,17 278 517,16 [M+Na]+
by UV spectrophotometry and/or electrospray ioniza- m/z values in TOF-MS mode and m/z identification are
tion time-of-flight mass spectrometery. ionization was given in Table. The elaborated methodology is destined
performed in positive ESi mode. Retention parameters for the quality assessment of pharmaceutical raw ma-
(capacity coefficients K), UV absorbance maxima (X ), terials and dietary supplements of herbal origin.
ASSESSMENT OF CORRELATION BETWEEN THE CONTENT OF POLYPHENOLS, PROANTHOCYANIDINS AND ANTIOXIDANT ACTIVITY IN "IN VITRO" TESTS IN FOOD AND MEDICINAL RAW PLANT MATERIALS
© Perova I. B., Eller K. I.
Institute of Nutrition RAMS, Moscow, Russia
Proanthocyanidins (OPC) or oligomers of cate-chins (flavan-3-ols) are among the most widespread forms of polyphenolic compounds of plant origin. According to the U. S. database (USDA Database for the Proanthocyanidin content of selected foods, 2004), proanthocyanidins provide more than 50 % of the
daily dietary intake of polyphenols. OPCs are widely used in food dietary supplements, medicinal and prophylactic nutrition and pharmaceuticals, enriched by antioxidants. The aim of this study was to investigate the correlation between the total content of polyphenolic compounds in g of gallic acid equivalent (GAE),
Samples Polyphenols, % (g GAE/100g) OPC, % FRSA, trolox equivalent uM/g
Cinnamon ground 37,97 ± 0,55 5,13 ± 0,09 77750 ± 380
Lingonberry leaves 13,48 ± 0,04 3,98 ± 0,22 2560± 130
Green tea powdered leaves 6,65 ± 0,26 0,49 ± 0,10 2570± 145
Birch buds 5,58 ± 0,04 0,21 ± 0,02 2555 ± 125
Origanum herb Herba origani vulgaris 3,66 ± 0,04 0,05 ± 0,01 2580 ± 140
Oak bark 3,53 ± 0,06 1,05 ± 0,14 2632±130
Green tea leaves 3,44 ± 0,06 0,22 ± 0,05 2543± 145
Marsh cinquefoil stems Stipes Comari palustris 2,97 ± 0,06 2,37 ± 0,10 2565±150
Saint-John's wort herb 2,58 ± 0,09 2,02 ± 0,06 2540± 135
Cocoa beans 2,39 ± 0,02 1,54 ± 0,09 4375± 170
Rose hips 2,21 ± 0,04 0,34 ± 0,06 2580± 115
Willow herb Herba Epilobii 1,45 ± 0,06 0,13 ± 0,03 2400± 140
Walnuts 1,44 ± 0,06 0,11 ± 0,03 2590±120
Nutmeg 1,13 ± 0,04 1,04 ± 0,12 2005± 110
Bilberry (wild) 0,99 ± 0,09 0,24 ± 0,05 3300± 170
Red kidney beans 0,95 ± 0,14 0,45 ± 0,08 10600±240
Walnuts septum 0,68 ± 0,07 0,42 ± 0,06 1620±100
Red grape peel 0,47 ± 0,07 0,8 ± 0,09 870 ± 95
Sour cherry 0,44 ± 0,05 0,36 ± 0,06 980 ± 90
Viburnum opulus berries 0,43 ± 0,04 0,19 ± 0,03 1150 ± 90
Black currant 0,16 ± 0,03 0,28 ± 0,03 200 ± 70
ТОМ 10/2012/2 Obzory po kliniceskoj farmacologii i lekarstvennoj terapii [Reviews of clinical pharmacology and drug therapy] И ^^ 49
concentration of OPCs and antiradical activity in tests "in vitro". More than 40 samples of food and medicinal plants were investigated. Total polyphenolics were determined employing Folin-Ciocalteu photometric method, sum of OPC — by Bate-Smith acid butanol assay, free radical scavengic activity (FRSA) — by the DPPH test.
The study was carried out to search the antioxidant ingredients for diet enrichment. No direct correlation between the antioxidant in vitro tests and the content of poly-phenolic compounds and proanthocyanidins has been found. On the basis of the above test results the feasibility of using products based on cinnamon, red beans, cocoa and blueberry for diet enrichment has been shown.
IN VITRO CYTOTOXIC AND ANTIOXIDANT ACTIVITIES OF SOME PLANT EXTRACTS ON DIFFERENT HUMAN CANCER CELL LINES
© El-Hallouty Salwa M.1, Khalifa Noha S.2, Barakat H. M. 2, Salim Dina 2
1Pharmacognosy Department, National Research Centre, Giza, Cairo, Egypt 2Botany Department, Ain Shams University, Cairo, Egypt
This study involves a bioassay screening of 22 methanol extracts from 19 plants that are commonly used in Egypt for many purposes based on their ethnic values. The aim of this study is to evaluate the cytotoxic and antioxidant activities of 22 methanol extracts. The methanol plant extracts were tested in vitro against four human cancer cell lines (by using MTT method) to determine their cytotoxic effect. The cell viability was examined after 24 h exposure to 100 ^g/ml of the extract in the medium. Negative (dimethyl sulfoxide) and positive (An-nonacherimolia methanol extract) controls were simultaneously used. Moreover, the antioxidant effect was determined using DPPH assay. Extracts showing cytotoxic and antioxidant activities were further subjected to determine their (lethal concentration) LC50 and LC90 values. Confirmed in vitro cytotoxic activity was found in four plant extracts. in HepG-2, Cympopogonproximus, Perseaamericana fruits and Vignaunguiculataseeds showed LC50 = 57.4, 13.3 and 56.4, respectively. in
A-549, Perseaamericana fruits and Tabernamontana-divaricata leaves showed LC50=35.4 and 70.7, respectively. in HT-29, Cympopogonproximus, Perseaameri-cana fruits and Tabernamontanadivaricata leaves showed LC50 = 58.6, 22 and 67.5, respectively. in MCF-7, Perseaamericana fruits showed LCm = 54.5. In vitro an-
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tioxidant activity was confirmed in three plant extracts Ceratoniasiliqua leaves, Perseaamericana leaves, Abrusprecatorius seeds showed LC50 = 10.6, 46.5 and 25.7, respectively. 5 Extracts out of the 22 studied methanol extracts exhibited potent antioxidant properties when tested at the concentration of 100 ppm against DPPH. The methanol extract of Persea americana leaves, Abrus precatorius seeds, Ceratonia siliqua leaves had LC50=46.5, 25.7, 10.6 ^g/ml, respectively Table 4. The methanol extracts of Ocimum basillicum and Hilianthus annuus had lost their activity below 100 ppm. The plants under study may represent promising natural sources for cytotoxic and antioxidant drug discovery.
PHYTOCHEMICAL SCREENING AND ANTIOXIDANT CAPACITIES OF SOME COMPOSITAE PLANTS
© Ergene B.-1, Bahadir Acikara Ö.1, Bakar F.2, Saltan G., Nebioglu S. 2
1Ankara University Faculty of Pharmacy, Department of Pharmacognosy, Ankara, Turkey 2Ankara University Faculty of Pharmacy, Department of Biochemistry, Ankara, Turkey
Asteraceae is one of the families which include many members containing phenolic compounds (1). The beneficial effects of many of the phenolics in human health have been attributed to their reactive oxygen and nitrogen scavenging and antioxidant capacity. The consumption of vegetables, fruits and flavonoid-rich beverages has been reported to prevent against neurodegenerative diseases, cancer and aging (2). Phenolic compounds have antioxidant potential due to their tendency to act as reducing agents, hydrogen donors, sin-
glet oxygen quenchers and metal chelators, chelating agents which can bind metal ions, could be added to enhance the activity of natural preservatives in food stuffs (2, 3, 4). in this study, the antioxidant activity of three members of Asteraceae family; Crepis foetida subsp. rhoeadifolia, Leontodon crispus var. asper and Pilosella hoppeana subsp. testimonials were evaluated using 1,1-diphenyl-2-picrylhydrazyl (DPPH) free radical scavenging assay and measuring malondialdehyde measuring levels. in DPPH free radical scavenging activity as-
fM 50 ® Obzory po kliniceskoj farmacologii i lekarstvennoj terapii [Reviews of clinical pharmacology and drug therapy]
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