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EC50OH = 9.0 mg/ml) increased with the addition of plums. The obtained results indicate that the honey with
20 % (EC50O^ = 27.4 mg/ml; EC50OH = 2.7 mg/ml), 30 % dried plums is a new product with high antioxidant activ-
(EC50O2^ = 28.0 mg/ml; EC50OH = 3.2 mg/ml) and 40 % ity and their inclusion in the diet may be recommended
(EC50O2^ = 28.8 mg/ml; EC50 OH = 7.9 mg/ml) of dried to complement other polyphenol sources.
RESEARCH OF EXTRACTION CONDITIONS OF PEPPERMINT AND BOGBEAN RAW MATERIALS AS THE ACTIVE COMPOUNDS OF HERBAL MEDICAL PRODUCT "TRIVALUMEN FORTE"
© Dobrovolnyi O. O. ', ShalamayA. S. Krutskih T. V.2, SlobodianiukI.O2
1PJSC SIC "Borshchahivskiy CPP", Kyiv, Ukraine 2National University of pharmacy, Kharkiv, Ukraine
Peppermint and bogbean leaves use for preparation of the active substance of the herbal medical product "Trivalumen". The therapeutic role of these components is antispasmodic and choleretic action of phenolic compounds and secretolytic action of iri-doids. The works purpose was determination of the optimal extraction conditions of these raw materials in process of herbal medical product "Trivalumen Forte". Raw material was extracted separately by different solvents (93 %, 70 %, 40 % ethanol and water) till the total DER 1:10. Sampling was carried out at interval DER 1:1 for each experiment. in the peppermint and bogbean extracts the amount of total flavonoids was determinated by spectrophotometry. The composition of flavonoid fraction of the peppermint extracts and presence of loganin in the bogbean extracts was identified by TLC. The efficacy of extraction process was
assessed using experimental data. The dependency of extraction dynamic of total flavonoids and extract-able substances were represented vs. extragent polarity and DER. The optimal conditions of peppermint extract for herbal medical product "Trivalumen Forte" was extraction of raw material by 40 % ethanol at DER 1:7-8. The extract contained not less than 9 % of total of flavonoids in equivalents of gesperidin (dry extract) and composition of this extract include rutin, gipero-side, quercitin, chlorogenic and caffeic acids. Yield of extractable substances was not less than 20 %. For bogbean extract the optimal extraction conditions of raw material was by 40 % ethanol at DER 1:6-7. This extract contained not less than 2.5 % of total flavonoids in equivalents of rutin (dry extract) and characterized by the presence of loganin. Yield of extractable matters was not less than 30 %.
THE METODOLOGICAL APPROACH TO THE IRIDOID ANALYSIS IN HERBAL RAW MATERIALS
© Zhogova A. A., Eller K. I.
Institute of Nutrition RAMS, Moscow, Russia
iridoids are a large group of cyclopentapyran monoterpenoids widely distributed in nature. iridoids are represented mainly in dicotyledonous plant families namely Plantaginaceae, Lamiaceae, Asteraceae, Gentianaceae, Rubiaceae, Oleaceae. Medicinal plants containing iridoids have a long history of use in the official and folk medicine as bitter tonics, choleretics, anti-inflammatory and antimicrobial agents, remedies for wounds and skin disorders. Recent studies have shown antioxidant, neuro-, hepato-, cardioprotec-tive and adaptogenic properties of iridoids. A number of iridoidscontaining medicinal plants are included in National Pharmacopoeias of leading countries of the world. in Russian Federation adequate and maximum acceptable intake levels for iridoids (aucubin, harpa-goside, oleuropein, asperulosidic deacetylasperulo-
sidic acid) were established by «Unified sanitary-ep-idemiological and hygienic requirements for goods subject to sanitary and epidemiological surveillance (monitoring)». Standardization of iridoids containing herbal materials is hindered by lack of reliable methods for determination of specific indicative iridoids such as aucubin and catalpol for genus Plantago and Veronica, harpagide, leonuride for Leonurus (Lamiaceae), oleuropein for Olea (Oleaceae), loganin for Menyanthus (Menyanthaceae), asperulosidic and deacetylasperu-losidic acid for Morinda (Rubiaceae), etc. A quantitative HPLC-DAD/ESi-TOF-MS method was developed for the simultaneous determination of iridoids mentioned above. Optimal chromatographic conditions were achieved using gradient elution with 0.1 % aqueous formic acid and methanol. Analytes were identified
fM 48 ® Obzory po kliniceskoj farmacologii i lekarstvennoj terapii [Reviews of clinical pharmacology and drug therapy]
TOM 10/2012/2
Compound К UV (nm) ESI-MS+ m/z Ion detected
Catalpol 1,20 200 385,11 [M+Na]+
Deacetylasperulosidic acid 1,41 235 413,10 [M+Na]+
Aucubin 1,75 200 369,12 [M+Na]+
Asperulosidic acid 2,87 235 455,11 [M+Na]+
Harpagide 2,37 200 387,13 [M+Na]+
Leonuride 2,43 200 371,13 [M+Na]+
Galiridoside 2,57 200 369,12 [M+Na]+
Loganin 3,45 235 413,15 [M+Na]+
Acetylharpagide 3,50 200 429,14 [M+Na]+
Ajugoside 3,67 200 413,14 [M+Na]+
Oleuropein 4,29 235 563,19 [M+Na]+
Harpagoside 5,17 278 517,16 [M+Na]+
by UV spectrophotometry and/or electrospray ioniza- m/z values in TOF-MS mode and m/z identification are
tion time-of-flight mass spectrometery. ionization was given in Table. The elaborated methodology is destined
performed in positive ESi mode. Retention parameters for the quality assessment of pharmaceutical raw ma-
(capacity coefficients K), UV absorbance maxima (X ), terials and dietary supplements of herbal origin.
ASSESSMENT OF CORRELATION BETWEEN THE CONTENT OF POLYPHENOLS, PROANTHOCYANIDINS AND ANTIOXIDANT ACTIVITY IN "IN VITRO" TESTS IN FOOD AND MEDICINAL RAW PLANT MATERIALS
© Perova I. B., Eller K. I.
Institute of Nutrition RAMS, Moscow, Russia
Proanthocyanidins (OPC) or oligomers of cate-chins (flavan-3-ols) are among the most widespread forms of polyphenolic compounds of plant origin. According to the U. S. database (USDA Database for the Proanthocyanidin content of selected foods, 2004), proanthocyanidins provide more than 50 % of the
daily dietary intake of polyphenols. OPCs are widely used in food dietary supplements, medicinal and prophylactic nutrition and pharmaceuticals, enriched by antioxidants. The aim of this study was to investigate the correlation between the total content of polyphenolic compounds in g of gallic acid equivalent (GAE),
Samples Polyphenols, % (g GAE/100g) OPC, % FRSA, trolox equivalent uM/g
Cinnamon ground 37,97 ± 0,55 5,13 ± 0,09 77750 ± 380
Lingonberry leaves 13,48 ± 0,04 3,98 ± 0,22 2560± 130
Green tea powdered leaves 6,65 ± 0,26 0,49 ± 0,10 2570± 145
Birch buds 5,58 ± 0,04 0,21 ± 0,02 2555 ± 125
Origanum herb Herba origani vulgaris 3,66 ± 0,04 0,05 ± 0,01 2580 ± 140
Oak bark 3,53 ± 0,06 1,05 ± 0,14 2632±130
Green tea leaves 3,44 ± 0,06 0,22 ± 0,05 2543± 145
Marsh cinquefoil stems Stipes Comari palustris 2,97 ± 0,06 2,37 ± 0,10 2565±150
Saint-John's wort herb 2,58 ± 0,09 2,02 ± 0,06 2540± 135
Cocoa beans 2,39 ± 0,02 1,54 ± 0,09 4375± 170
Rose hips 2,21 ± 0,04 0,34 ± 0,06 2580± 115
Willow herb Herba Epilobii 1,45 ± 0,06 0,13 ± 0,03 2400± 140
Walnuts 1,44 ± 0,06 0,11 ± 0,03 2590±120
Nutmeg 1,13 ± 0,04 1,04 ± 0,12 2005± 110
Bilberry (wild) 0,99 ± 0,09 0,24 ± 0,05 3300± 170
Red kidney beans 0,95 ± 0,14 0,45 ± 0,08 10600±240
Walnuts septum 0,68 ± 0,07 0,42 ± 0,06 1620±100
Red grape peel 0,47 ± 0,07 0,8 ± 0,09 870 ± 95
Sour cherry 0,44 ± 0,05 0,36 ± 0,06 980 ± 90
Viburnum opulus berries 0,43 ± 0,04 0,19 ± 0,03 1150 ± 90
Black currant 0,16 ± 0,03 0,28 ± 0,03 200 ± 70
ТОМ 10/2012/2 Obzory po kliniceskoj farmacologii i lekarstvennoj terapii [Reviews of clinical pharmacology and drug therapy] И ^^ 49
