CC BY
22B-O-1
BIOMEDICAL PHOTONICS
Viscoelasticity measurement using laser speckle techniques
Jiachi Hong1, Xiao Chen1, Jinling Lu1, Alexander Priezzhev3, Andrei E. Lugovtsov3,
Pengcheng Li12
'Britton Chance Center for Biomedical Photonics, Wuhan National Laboratory for Optoelectronics-Huazhong University of Science and Technology, Wuhan, 430074, China 2School of Biomedical Engineering, Hainan University, Haikou, 570228, China
3 Department of Physics, LomonosovMoscow State University, Leninskye Gory, '-62, Moscow, ''999', Russia;
pengchengli@mail.hust.edu.cn
Viscosity and elasticity are important physiological characteristics of biological tissues. Viscoelastic measurement is of great significance for assessing tissue functions and deciphering pathological mechanisms, especially in blood microcirculation. Magnetic resonance elastography (MRE) and ultrasound elastography (UE) have been widely applied to clinical diagnosis. Compared to MRE and UE, optical elastography methods, such as optical coherence elastography (OCE) and laser speckle elastography (LSE), are getting more and more attention for the advantages of high spatial resolution and motion detection sensitivity. Laser speckle imaging can detect the scattered particles velocity related to the strain by solving the motion-induced decorrelation time with speckle statistical analysis. Although LSE has a wide field and high temporal resolution, there are still some limitations for clinical application scenarios. Therefore, we first estimate the apparent viscosity of blood microcirculation by analyzing the blood flow velocity with laser speckle imaging. Then, we develop a laser speckle elastography method with a low-frame-rate camera by using the aliasing effect. It simplifies the complexity of the elastography system and enhances portability due to the no need for synchronization between excitation and acquisition [1]. Further, as biological tissue is both elastic and viscous, ignoring the viscosity of the tissue can make elastic measurements inaccurate. We accomplish the simultaneous quantitative detection of viscosity and elasticity based on reflective laser speckle contrast imaging [2]. The viscosity and elasticity moduli are obtained by fitting the Rayleigh wave dispersion curves to the Voigt model. Compared with results obtained from a conventional mechanical rheometer, both phantom and biological experiment results support our method for viscoelasticity measurement.
This work was supported by the National Natural Science Foundation of China (6'89095', 6'890950, 6'77507'); CAMS Innovation Fund for Medical Sciences (CIFMS, 20'9-I2M-5-0'4); Fundamental Research Funds for the Central Universities, HUST: 20'9KFYXMBZ009); Innovation Fund of Wuhan National Laboratory for Optoelectronics (WNLO)
[1] Xiao Chen, Jinling Lu, and Pengcheng Li, "Elastography with low-frame-rate laser speckle contrast imaging using the aliasing effect," Opt. Lett. 43, 2811-2814 (2018)
[2] Xiao Chen, Yang Wang, Jinling Lu, and Pengcheng Li, "Simultaneous viscosity and elasticity measurement using laser speckle
contrast imaging," Opt. Lett. 43, 1582-1585 (2018)
ALT'22
