CC BY
11B-I-4
Laser speckle techniques for accessing biological function
P. Li1, J. Lu1, J. Hong1, X. Chen1
1Huazhong University of Science and Technology,
Wuhan National Laboratory for Optoelectronics, Wuhan, China
Laser speckle is a phenomenon that will be produced when a coherent light is illuminated to biological tissues. Since the laser speckle is sensitive to the motion and deformation of tissue, laser speckle related techniques are widely attempted to estimate the mechanic and rheological properties of biological tissue, such as blood flow speed, viscosity, elasticity, diffusivity, viability and so on. Moreover, the blood volume and oxygenation can also be obtained by using multiple wavelengths. By combined with the imaging of either endogenous or exogenous fluorescence, we can further access the information of molecule inside the biological tissue. Laser speckle contrast imaging (LSCI) is a full-field, non-contact and low cost optical imaging method for monitoring blood flow and vascular morphology, which is attracting increasing applications in biomedical researches and clinics. Here, the methodology of laser speckle techniques for evaluation of tissue function are discussed, such as the quantitative model for temporal speckle contrast imaging of blood flow, the estimation of viscoelasticity using laser speckle, the imaging processing strategy for the denoising of laser speckle contrast imaging, and the spatial selective regulation of blood flow using wave front engineering and laser speckle. The applications of laser speckle on diagnosis and therapy of skin diseases, burn, and nerve block are also presented.
