Научная статья на тему 'Ultrastructure and phylogeny of new species of Glugea infecting the intestinal wall of Cephalopholis hemistiktos in Saudi Arabia'

Ultrastructure and phylogeny of new species of Glugea infecting the intestinal wall of Cephalopholis hemistiktos in Saudi Arabia Текст научной статьи по специальности «Биологические науки»

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Текст научной работы на тему «Ultrastructure and phylogeny of new species of Glugea infecting the intestinal wall of Cephalopholis hemistiktos in Saudi Arabia»

Protistology 10 (2), 5-93 (2016) Protistology

Abstracts of the International Scientific Forum "PROTIST-2016"

ULTRASTRUCTURE AND PHYLOGENY OF NEW SPECIES OF GLUGEA INFECTING THE INTESTINAL WALL OF CEPHALOPHOLIS HEMISTIKTOS IN SAUDI ARABIA Abdel-Gawwad Abdel-Azeem12, Al-Quraishy Saleh1, Azevedo Carlos31

1 - Zoology Department, College ofScience, King Saud University, Riyadh, Saudi Arabia.

2 - Zoology Department, Faculty ofScience, Beni-Suef University, Egypt.

3 - Laboratory ofCell Biology, Institute ofBiomedical Sciences, University of Porto, Porto, Portugal az,[email protected]

A new microsporidian species of the genus Glugea Thelohan, 1891 parasitizing the marine teleost fish Cephalopholis hemistiktos Ruppell, collected from the Red Sea in Saudi Arabia, is described on the basis ofmicroscopic and molecular procedures. Spherical and whitish xenoma were observed adhering to the intestinal wall. The numerous spores contained within these xenoma, were ovoid to pyriform and measured 5.1 (4.3-6.0) ^m in length and 2.2 (1.82.9) ^m in width. The spore's wall was composed of two thick layers, which were thinner in the area contacting the anchoring disk. The latter appeared at the spore's anterior pole, in an eccentric position to the longitudinal axis. A lamellar polaroplast surrounded the uncoiled portion of the polar filament projected to the basal region of the spore, giving rise to 26-29 turns with winding from the base to the anterior zone of the spore. The posterior vacuole, located at the spore's posterior pole, and surrounded by the polar filament coils, was irregular and composed of light material. Molecular analysis of the rRNA genes, including the ITS region, was performed using maximum parsimony, neighbour-joining and maximum likelihood methods. The ultrastructural features observed, combined with the phylogenetic data analyzed, suggest this parasite to be a new species of the genus Glugea.

BIODIVERSITY AND COMMUNITY STRUCTURE OF SOIL CILIATES COLLECTED FROM THREE DIFFERENT SITES OF DELHI, INDIA TO ASSESS SOIL QUALITY Abraham J.S.1, Somasundaram S.1, Jangra S.1, Yadav K.1, Singh S.1, Zutshi S.1, Singh B.1, Dagar J.1, Kumar A.1, Goyal A.1, Bhatnagar M.1, Upadhaya M.1, Choudhary A.1, Toteja R.1, Gupta R.2, Gambhir G.1, Makhija S.1

1 - Acharya Narendra Dev College, University ofDelhi, Delhi, India

2 - Maitreyi College, University ofDelhi, Delhi, India [email protected], [email protected] Soil is a natural body ofmineral and organic material differentiated into horizons, which differ among themselves as well as from underlying materials in their morphology, physical make-up and chemical composition. Biological components ofthe soil can vary from one site to another site depending upon the inorganic and organic make up of the soil. Soil ciliates are an integral part of the soil community. They play an important role in nutrient recycling by feeding on bacteria. Also, they are known to stimulate ammonification and nitrification that can later be used by plants and other members of the food web. Although they play an influential ecological role, information on soil ciliate diversity is still rudimentary. Particularly from India, very few data are available on soil ciliates. In this present study, the ciliate diversity was investigated from three different areas, that is, from human inhabitant land [Acharya Narendra Dev College (ANDC) campus], an agricultural land (Karnal) and a sewage treatment plant (Rithala) within Delhi, India. Physicochemical analysis of soil samples was conducted for its pH, nitrogen content, organic carbon content. Ciliate abundance was correlated with physicochemical properties. In total ciliates belonging to 5 Classes, 7 Orders, 15 genera and 20 species were found with maximum number

© 2016 The Author(s)

Protistology © 2016 Protozoological Society Affiliated with RAS

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