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Protistology 6 (4), 264-270 (2010/11)
Protistology
Two new species of Myxobolus (Myxosporea, Bivalvulida) from the Indian major carp Labeo rohita (Ham.)
Harpreet Kaur and Ranjeet Singh
Department of Zoology, Punjabi University, Patiala, India
Summary
During a survey on fish parasites in Wetlands of Punjab, India, two new myxosporean species, Myxobolus sushmii sp. nov. and Myxobolus punjabii sp. nov. were found infecting the Indian freshwater fish Labeo rohita (Ham.). Spores of M. sushmii sp. nov., a parasite infecting the eye ball, are measuring 9.0-10.2 (9.6 ± 0.84) x 7.3-9.3 (8.3 ± 1.41) ^m in size. The spores have ovoidal to sub-spherical shape with flat anterior end between the openings of two polar capsules and rounded posterior end. Spore valves are thick with 6-8 parietal folds arranged around the margin of spore. Two polar capsules are distinctly unequal, broad and pyriform in shape with pointed anterior end. Larger one is 4.1-5.3 (4.70 ± 0.84) x 2.9-3.5 (3.2 ± 0.42) and smaller one is 2.9-3.4 (3.10+0.35) x 1.0-3.0 (2.0 ± 1) ^m in size. Prominent U-shaped intercapsular process is present. M. punjabii sp. nov. infect the caudal fin. The sub-spherical spores are measuring 8.1-9.7 (8.9 ± 3.1) x 6.4-7.5 (6.9 ± 0.77) ^m in size. Shell valves are without parietal folds. Two polar capsules are distinctly unequal. Larger one measures 3.4-5.4 (4.4 ± 1.41) x 2-3.4(2.7 ± 0.98) ^m; smaller one is 2.8-3.6 (3.2 ± 0.56) x 1.2-2.2 (1.7 ± 0.7) ^m in size. They are tear shaped with pointed anterior end and broad posterior one. Both are placed parallel to each other. Intercapsular process is absent.
Key words: freshwater fishes, Harike Wetland, India, Kanjali Wetland, Labeo rohita, myxozoans, Myxobolus
Introduction
Myxozoans are economically important group of microscopic metazoan parasites, which cause disease in a large variety of commercially important fishes. They have been also reported in platyhelminthes, reptiles, amphibians, mammals and were also
detected in fecal sample of human beings (Boreham et al., 1998). As many as 104 species of the genus Myxobolus have been reported from Indian fishes (Kalavati and Nandi, 2007), however, myxozoan parasites infecting freshwater fishes of North India are studied insufficiently. The genus Myxobolus combines the most common myxosporeans para-
© 2010 by Russia, Protistology
sitizing fishes. Eiras with co-authors (2005) listed approximately 744 species of Myxobolus all over the world. In the present study, two new species belonging to the genus Myxobolus Butschli isolated from eye ball and caudal fin of Labeo rohita (Ham.) catched in Wetland, Punjab were described in accordance with the guidelines by Lom and Arthur (1989).
Material and methods
Infected eye and caudal fins were smeared on the clean slides in a drop of 0.98% NaCl solution, covered with cover slip and examined for the presence of spores. To observe iodinophilous vacuole the smears were immersed in Lugol’s iodine solution. To stimulate the filament extrusion the smears were treated with 8% KOH. For permanent preparation, the smears were fixed in Bouins fixative and stained with Ziehl-Neelsen and Iron-haematoxylin. Drawings were made from stained material with the aid of camera lucida. 15-20 spores were measured with the aid of a calibrated ocular micrometer. All measurements are given in microns (^m) as range values followed by mean ±SD in parentheses.
Results
Myxobolus sushmii sp. nov. (Figs 1-3, 8-10).
Plasmodia. Creamy- white, large, oval to spherical in shape plasmodia are measuring 2x3 mm in size, 2-3 in number in each eye ball. 60- 65 spores are present per plasmodium.
Spores. The spores are histozoic, ovoidal to sub-spherical in shape measuring 9.0-10.2 (9.6+0.84) x 7.3-9.3 (8.3+1.41) ^m in size and lenticular in lateral view. The anterior end is flat between the openings of two polar capsules and posterior end is broad and rounded. Spore valves are thick, symmetrical, measuring 1.0 ^m in thickness, with 6-8 parietal folds arranged all around the margin of spore. Two polar capsules are distinctly unequal. Larger one is measuring 4.1-5.3 (4.70+0.84) x 2.9-3.5 (3.20+0.42) ^m and smaller one is 2.9-3.4 (3.10+0.35) x 1.03.0 (2.0+1) ^m in size. Both are broadly pyriform with pointed anterior end and rounded posterior one. The capsules open independently. Both polar capsules are situated parallel to each other in spore cavity. Larger one occupies half of the spore body; the smaller one — one third of it. Large prominent U- shaped intercapsular process is present. In
Figs 1-3. Photomicrographs of M. sushmii sp. nov.: 1, 2 - spores stained in Ziehl-Neelsen; 3 -spores with the polar filaments extruded through the independent openings stained with Iron-haematoxylin. Scale bars = 10 ^m.
larger capsule polar filament is coiled into 4-6 turns compared to 3-4 turns in smaller one. The coils are aligned obliquely to the longitudinal axis of the capsule. The extruded polar filaments are unequal in length and in thickness, the thick one is measuring 48.3 ^m and the thin one is 30 ^m in length. Two capsulogenic nuclei measuring 0.861.46 (1.16+0.42) ^m in diameter, are present just beneath each polar capsule. Sporoplasm is agranular, homogenous, hemispherical and contains two sporoplasmic nuclei measuring 0.83 ^m in diameter. Iodinophilous vacuole is absent.
Myxobolus punjabii sp. nov. (Figs 4-7, 11-13).
plasmodium. White, small, rounded plasmodia measuring 0.08x1.0 mm in size, 4-5 in number are found attached to each caudal fin. 30-35 spores are present per plasmodium.
spores. The spores are histozoic and measuring 8.1-9.7 (8.9+1.31) x 6.4-7.5 (6.9+0.77) ^m in size. They are sub-spherical in shape with rounded
Figs 4-7. Photomicrographs of M. punjabii sp. nov.: 4, 5- spores with the polar filaments extruded through the independent openings stained with Iron-haematoxylin; 6, 7- spores stained in Ziehl-Neelsen. Scale bars = 10^m.
anterior and posterior ends. In sutural view, spores are lenticular and shell valves are protruded towards outside at anterior end and pointed slightly curved at posterior end. Shell valves are thick, smooth, symmetrical, measuring 1.16 ^m in thickness. Two polar capsules are distinctly unequal, tear shaped with sharp pointed tip at the anterior end and broad posterior end. Larger polar capsule measures 3.4-5.4 (4.4+1.41) x 2-3.4 (2.7+0.98) ^m; smaller one is 2.83.6 (3.2+0.56) x 1.2-2.2 (1.7+0.7) ^m in size. The polar capsules are situated parallel to each other. The larger one is coiled into 6-7 turns while the smaller one — into 4-5 turns, which aligned perpendicular to the axis of polar capsule. Polar filaments are thin, unequal in length when extruded. They are open independently and measuring 57-59.6 (58.3+0.18) and 13-15(14+1.41) ^m, respectively. Intercapsular
process is absent. Two capsulogenic nuclei are present beneath each polar capsules, measuring
1.50 ^m in diameter. Sporoplasm is agranular and homogenous occupying all extracapsular space behind the polar capsules. Iodinophilous vacuole is large and measuring 2.19-4.13 (3.16+1.37) ^m in diameter. Two sporoplasmic nuclei are measuring
1.50 ^m in diameter.
Discussion
Myxobolus sushmii sp. nov. While comparing M. sushmii sp. nov. with M. maggauddi Bajpai et al., 1981 from branchial filaments of Trichogaster fasciatus; M. chakravartyi Haldar et al., 1983 from internal musculature of Catla catla; M. bliccae Donec and Taoziyakova (in Shulman, 1984) from gills of Abramis sapa and Blicca bjoerkna; M. njinei Fomena et al., 1985 from gill arch and connective tissue of Barbus camptacanthus; M. mahendrae Sarkar, 1986 from gill arch of Catla catla; M. punjabensis Gupta and Khera, 1989 from fins and gills of Labeo dyocheilus; M. bilongi Fomena et al., 1994 from gills and fins of Labeo sp.; M. labeosus Sarkar, 1995 from mesentery associated with spleen of L. fimbriatus, it was found that present species differed from all of the above mentioned species in the morphometric characteristics (Table 1).
Spores of M. sushmii sp. nov. are ovoidal to sub-spherical in shape having anterior end flattened between two opening of the polar capsule and rounded posterior end. Its shape is different from oval spores of M. maggauddi; bowl-shaped spores of M. punjabensis and oval to egg-shaped but obliquely truncated (anteriolaterally) spores of M. labeosus. The present species have 6-8 parietal folds arranged all around the margins of spore in contrast to 5-6 in M. mahendrae restricted to the posterior end of shell valves and its absence in M. chakravartyi, M. bilongi, M. bliccae and M. njinei. The shape of the spores of the present species M. sushmii sp. nov. closely resemble to that of M. mahendrae and M. chakravartyi, however, differed in having a well developed U- shaped intercapsular process.
In light of above differences from closely related species the present myxozoan was considered as a new species.
Myxobolus punjabii sp. nov. The shape and size of spores of the described species was compared with the spores of M. amurensis Akhmerov,1960 from fins and gut of Cyprinus carpio haematopterus; M. sphaerocapsularis Shulman, 1962 from muscles of Acheilognathus chankaensis; M. diversicapsularis
Figs 8-10. Camera lucida drawing of the spores of M. sushmii sp. nov.: 8 - mature spore stained in Ziehl-Neelsen (valvular view); 9 - spore with extruded polar filaments; 10 - spore in sutural view. Scale bar = 5^m.
Slukhai (in Shulman, 1966) from gills of Rutilus rutilus; M. rachmani Allamuratov, 1966 from kidneys of Alburnoides tenniatus; M. musseliusae Yakovchuk, 1979 from gills of Cyprinus carpio; M. alburni Donec, 1984 from fins of Alburns alburns; M. gobiorum Donec, 1984 from fins of Gobio gobio; M. drjagini (Akhmerov, 1954) Landsberg and Lom, 1991 from skin of Hypophchthysthalmi and M. dossoui Sakiti et al., 1991 from gill arch cartilage of Tilapia zillii. The present species differed in the morphometric characteristics as well as in the shape of polar capsules from all the above mentioned species (Table 2).
The spores of M. punjabii sp. nov. are characterized by sub-spherical shape, thick and smooth shell valves without any parietal folds. Furthermore, the polar capsules are unequal, tear shaped with very sharp pointed anterior and broad rounded posterior ends, situated parallel to each other, containing 6-7 and 4-5 turns of the polar filaments. Both polar filaments are unequal and extrude independently through anterior end of spore. Therefore, in
Figs 11-13. Camera lucida drawing of the spores of M. punjabii sp. nov.: 11, 12 - spores stained in Ziehl-Neelsen (valvular view); 13 - spore in sutural view. Scale bar = 5^m.
this respect, they are closer to M. gobiorum, M. musseliusae and M. dossoui but differ from the above mentioned species in absence ofintercapsular process. The latter is very large in M. dossoui, medium-sized in M. gobiorum and M. musseliusae. In light of such differences we hereby propose this species as a new one.
Taxonomic summary of M. sushmii sp. nov.
Type host: Labeo rohita (Ham.).
Type locality: Harike Wetland Punjab, India. Type specimens: Paratypes are spores stained in Ziehl-Neelsen and Iron-haematoxylin deposited in the museum of Department of Zoology, Punjabi University, Patiala. No. LR/Q/ZN/17.01.2009 and LR/Q/IH/ 17.01.2009.
Table 1. Comparative description of the present Myxobolus sushmii sp. nov. with morphologically similar species.
Species Host Location Locality Spore (^m) Polar capsule (Mm)
M. sushmii sp. nov. (present study) Labeo rohita eye ball Harike Wetland, Punjab (India) 9.0-10.2 (9.6) x 7.3-9.3 (8.3) 4.1-5.3 (4.70) x 2.93.5 (3.20) and 2.9-3.4 (3.10) x 1.0-3.0 (2.0)
M. maggauddi Bajpai et al., 1981 Trichogaster fasciatus branchial filament West- Bengal (India) 10.8-11.7 (11.2) x 8.3-10.0 (9.2) 3.3-5.0 (4.0) x 2.5-3.3 (2.6) and 2.5-3.3 (2.6) x1.7-2.5 (1.8)
M. chakravarty Haldar et al., 1983 Catla catla internal- musculature West- Bengal (India) 12.1-14.3 (12.3) x 7.7-10.5 (8.8) 5.5-6.6 (5.6) x 3.3-5 (4.3) and 4.4-5 (4.5) x 2.2-4.4 (3.2)
M. bliccae Donec and Taoziyakova (in Shulman, 1984) Blicca bjoerkna; Abramis sapa gills Ukraine 10.3-14 x 8.211.8 5.5-7.9 x 3-4.5
M. njinei Fomena et al., 1985 Barbus sp. gill arch connective tissue Cameroon 14-20 x 11.518.5 6.5-9 x 3.5-5.5
M. mahendrae Sarkar, 1986 Catla catla gill arch epithelium India 11.5-14.0 (12.7) x 9.8-10.5 (10.4) 6.3-7.3 (7.0) x 3.5-4.2 (3.7)
M.punjabensis Gupta and Khera, 1989 Labeo dyocheilus gills, fins Ludhiana and Harike Wetland, Punjab (India) 10-12 (10.86) x 10-11 (10.64) 5-6 (5.72) x 4-5 (4.4) and 4-5 (4.72) x 3-4 (3.52)
M. bilongi Fomena et al., 1994 Labeo sp. gills, fins Cameroon 14-17 x 11.514.5 6.5-8 x 4-6 and 4-6.5 x 3-4
M. labeosus Sarkar, 1995 Labeo fimbriatus mesentry assoicated with spleen West -Bengal (India) 8.0-10.0 (9.25) x 6.5-9.0 (7.62) 6.0-6.5 (6.15) x 2.04.0 (2.72) and 3.0-4.5 (4.02) x 1.8-2.5 (2.33)
Site of infection: Eye ball.
Prevalence of Infection: 8/28 (28.5%).
Etymology: New species “sushmii” has been named in honor of Dr. Sushma Gupta, an alumnus of Punjab University, Chandigarh (India) who has significant contribution towards the study of myxozoan parasites of freshwater fishes of North India.
Taxonomic summary of M. punjabii sp. nov.
Type host: Labeo rohita (Ham.).
Type locality: Kanjali Wetland Punjab, India.
Type specimens: Paratypes are spores stained in Ziehl-Neelsen and Iron-haematoxylin deposited in the museum of Department of Zoology, Punjabi University Patiala, India. No. LR/P/ZN/10.05.2009 and LR/P/IH/ 10.05.2009.
Site of infection: Caudal fin.
Prevalence of Infection: 5/15 (33.3%).
Etymology: New species has been named as Myxobolus punjabii after the name of the place “Punjabi University Patiala” where the present work was carried out.
References
Akhmerov A.K. 1954. On closely related species of a new myxosporean genus. Dokl. Akad. Nauk SSR. 97, 1101-1103 (in Russian with English summary).
Akhmerov A.K. 1960. Myxosporidia of fishes of the Amur River Basin. Rybn. khoz. Vnutr. Vodoemov Latv. SSSR. 5, 240-307 (in Russian).
Allamuratov B. 1966. New species of parasitic protozoa found in the fish of South-Surkham Reservoir. Uzb. Biol. Zh. 10, 58-60 (in Russian with English summary).
Bajpai R.N., Kundu T.K. and Haldar D.P. 1981. Observations on Myxosoma maggaudii n. sp. (Myxozoa: Myxosomatidae) parasitic on the gill filaments of Trichogasterfasciatus Bl. Schn. Rivista di Parasitol. 3, 343- 350.
Boreham R.E., Hendrick S., O’Donoghue P.J. and Stenzel D.J. 1998. Incidental finding of Myxobolus spores (Protozoa: Myxozoa) in stool samples from patients with gastrointestinal symptoms. J. Clin. Microbiol. 36, 3728-3730.
Table 2. Comparative description of the Myxobolus punjabii sp. nov. with morphologically similar species.
Species Host Location Locality Spore (Mm) Polar capsule (Mm)
M. punjabii sp. nov. (present study) Labeo rohita caudal fin Kanjali Wetland Punjab (India) X ch'ch oo yo r-- un Ch r^ tH Tf oo 3.4-5.4 (4.4) x 2-3.4 (2.7) and 2.8-3.6 (3.2) x 1.2-2.2 (1.7)
M. amurensis Akhmerov, 1960 Cyprinus carpio haematopterus fins, gut Amur basin 9-13.5 x 9-12.5 4.5-7 x 3.8-4.2
M.sphaerocapsularis Shulman, 1962 Acheilognathus chankaensis muscles China 17-18 x 11-12 7-8 x 5.6-6.5
M. diversicapsularis Slukhai (in Shulman 1966) Rutilus rutilus gills Russia 8.5-13 x 8.5-12.5 3.8-5.5 x 2.2-3.7
M. rachmani Allamuratov, 1966 Alburnoides tenniatus kidney Central Asia 13.5-14.3 x 11.212 7.2-7.5 x 3.7-3.9
M. musseliusae Yakovchuk, 1979 Cyprinus carpio gills Russia 10.5-11.1 x 8.810 3.9-4
M. alburni Donec, 1984 Alburnus alburnus fins Russia 12-15.3 x 10-12 6.8-8.1 x 3-3.5
M. dossoui Sakiti et al., 1991 Tilapia zillii gill arch cartilage Benin 8.5-11 (9.9) x 8-10.5 (9.2) 4.5-6.5 (5.5) x 2.5-5 (3.1)
M. drjagini (Akhmerov, 1954) Landsberg and Lom, 1991 Hypophchthystha-lmi molitrix skin Amur Basin 13-14 x 9-10 5.5-6 x 3-3.5
M. gobiorum Donec, 1984 Gobio gobio fins Ukraine 11-13 x 9-10 5.2-6.5 x 2.2-3
Bütschli O. 1882. Myxosporidea. In: Bornn’s Klass Ordn., des Tierreiches, Protozoa. 1, 590603.
Donec Z.S. 1984. Parasitic Protozoa. In: Key to the parasites of freshwater fish of the USSR. (Ed.: Bauer O.N.). Leningrad, Nauka, 1. pp. 88-251 (in Russian).
Eiras J.C., Molnar K. and Lu Y.S. 2005. Synopsis of the species of Myxobolus Bütschli, 1882 (Myxozoa: Myxosporea: Myxobolidae). Sys. Parasitol. 61, 1-46.
Fomena A., Marques A., Bouix G. and Birge E. 1985. Contribution a l’ etude des Myxosporidies des Poissons d’eau douce du Cameroun. II. Especes nouvelles du genre Myxobolus Bütschli, 1882. Bull. I.F.A.N. 46, 167-192.
Fomena A., Marques A., Bouix G. and Njine T. 1994. Myxobolus bilongi n. sp., Thelohanellus assambai n. sp., T. sanagaensis n. sp., Myxosporidies parasites de Labeo sp. (Teleosteen, Cyprinidae) du Bassin de la Sanaga au Cameroun (Afrique Centrale). Annales de la Faculte des Sciences de Yaounde. I, (3), 131-142.
Gupta S. and Khera S. 1989. Observations on Myxobolus punjabensis sp. nov. (Myxozoa:
Myxobolidae), parasitic on gills and fins of Labeo dyocheilus. Rivista Di Parasitol. VI (L)-N. 1, 131138.
Haldar D.P., Das M.K. and Sharma B.K. 1983. Studies on protozoan parasites from fishes. Four new species of the genera Henneguya Thelohan, 1892, Thelohanellus Kudo, 1933 and Myxobolus Butschli, 1892. Arch. Protistenk. 127, 283-296.
Kalavati C. and Nandi N.C. 2007. Handbook of parasites of Indian fishes. ZSI. India, Kolkata.
Landsberg J.H. and Lom J. 1991. Taxonomy of the genera of the Myxobolus/Myxosoma group (Myxobolidae:Myxosporea), current listing of species and revision of synonyms. Syst. Parasitol. 18,155-186.
Lom J. and Arthur J.R. 1989. A guideline for the preparation of species descriptions in Myxosporea. J. Fish Dis. 12, 151-156.
Sakiti N., E. Blanc., Marques A. and Bouix G. 1991. Myxosporidies (Myxozoa: Myxosporea) du genre Myxobolus Butschli, 1882 parasites de poisons cichlidae du lae Nokoue au Benim (Afrique de l’ Ouest). J. Afr. Zool. 105, 173-186.
Sarkar N.K. 1986. On two new species of Myxobolus Butschli 1882 (Myxozoa: Myxosporea)
from the freshwater fishes of West-Bengal, India. Acta Protozool. 25, 235-239.
Sarkar N.K. 1995. Observations on two new myxosporidia (Myxozoa: Myxosporea) from fishes of Bhery-fishery of West-Bengal, India. Acta Protozool. 34, 67-70.
Shulman S.S. 1962. Phylum Protozoa. In: Key to parasites offreshwater fishes of the USSR. (Eds.: I.E. Bykhovskaya- Pavlovskaya et al.). Leningrad, Nauka. pp. 7-151 (in Russian).
Shulman S.S. 1966. The myxosporidian fauna of the USSR. Moscow-Leningrad, Nauka (in Russian).
Shulman S.S. 1984. Parasitic Protozoa. In: Key to parasites of freshwater fishes of the USSR. (Ed.: O.N. Bauer). Leningrad, Nauka, 1. pp. 252-280 (in Russian).
Yakovchuk T.A. 1979. A new species of the genus Myxobolus (Myxosporidia: Myxobolidae) from the gill filaments of the carp. Parasitologiya. 13, 635-636 (in Russian with English summary).
Address for correspondence: Harpreet Kaur. Department of Zoology, Punjabi University, Patiala-147002, India; e-mail: harpreet_bimbra@yahoo.com
