CC BY
4*
ALT'23 The 30th International Conference on Advanced Laser Technologies
B-O-5
Treatment of neoplasm using of dielectric nanoparticles doped with Yb3+ ions and non-contact exposure to 970-nm radiation
S.A. Khrushchalina1, I.A. Yurlov1, P.A. Ryabochkina1, O.A. Kulikov1, V.I. Shlyapkina1, V.P.
Ageev1, N.Yu. Tabachkova2
1- National Research Mordovia State University, 68 Bolshevistskaya Str., Saransk 430005, Republic of Mordovia,
Russia
2- National University of Science and Technology "MISIS", Leninskiy Prospect, 4, Moscow 119049, Russia
Main author email address: [email protected]
Excitation of dielectric nanoparticles highly doped with rare-earth (RE) ions by intense laser radiation can lead to their significant heating and, as a consequence, "white" emission [1-3]. This effect can be used to enhance the thermal effect of laser radiation on biological tissue. In [3], by means of in-vivo experiments (on rats), we demonstrated the fundamental possibility of using particles based on ytterbium-doped zirconium dioxide to enhance the thermal effect of radiation with a wavelength of 980 nm. In [4], we studied the possibility of using particles based on zirconium dioxide doped with various RE ions (Ho3+, Tm3+, Yb3+, Er3+) to enhance the heating of biological tissue by laser radiation of various wavelengths (457, 532, 980, 1550, 1940 nm). In-vivo experiments have shown that the effect is most pronounced for ytterbium-containing particles and radiation with a wavelength of 980 nm.
The purpose of this work was to study the possibility of using ytterbium-containing particles when they are excited by laser radiation with a wavelength of 970/980 nm for the treatment of subcutaneous neoplasms. In-vivo experiments were carried out (on mice of the BALB/c line). The tumor strain of melanoma B16-F10 was used for the study. Tumor-bearing animals were divided into four groups of six each. In the first group, animals with a tumor did not receive any treatment. In the second group, on the seventh day from the moment of tumor implantation, the animals were intratumorally injected with a suspension of Zr02-30 mol.% Yb2O3. In the third group of animals, a thick suspension of Zr02-30 mol.% Yb2O3 powder in distilled water was applied to the skin covering the tumor site. Animals of the second and third groups twice with an interval of 48 hours were exposed to the tumor node and adjacent areas with a laser with a wavelength of 970 nm and a power of 1 W. Animals of the fourth experimental group received a double heating of the tumor node to 60°C using laser radiation with a wavelength of 970 nm. In-vivo experiments have shown that therapy with a suspension of Zr02-30 mol% Yb2O3 particles (cutaneous or intratumoral location) has an antitumor effect against B16 melanoma cancer. In this case, a more effective therapeutic effect is achieved with intratumoral administration.
Experiments with the animals were carried out in accordance with the rules for working with animals formulated by Directive 2010/63/EU of the European Parliament and of the Council of the European Union on the protection of animals used for scientific purposes and were approved by the local ethics committee at the Medical Institute of National Research Mordovia State University, approval date: 9 May 2020, approval code: 88.
The transmission electron microscopy structural studies were conducted on equipment of the Materials Science and Metallurgy Joint Use Center with State financial support from the Ministry of Education and Science of the Russian Federation, Grant No. 075-15-2021-696. This work is financially supported by a grant from the Russian Science Foundation (Project 23-72-01099).
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[3] P.A. Ryabochkina, S.A. Khrushchalina, A.N. Belyaev, O.S. Bushukina, I.A. Yurlov, S.V. Kostin, Use of dielectric nanoparticles doped with Yb3+ ions to enhance the thermal effect in a biological tissue exposed to near-IR laser radiation (in vivo experiments), Quant. Electron., vol. 51, pp. 10381043, (2021).
[4] .A. Khrushchalina, I.A. Yurlov, P.A. Ryabochkina, V.P. Ageev, O.A. Kulikov, V.I. Shlyapkina, M.N. Tremasov, M.N. Zharkov, A.N. Belyaev, O.S. Bushukina, The use of dielectric nanoparticles doped with rare-earth ions to increase the thermal effect of laser radiation of various wavelengths on biological tissues, 2022 Internat. Confer. Las. Opt. (ICLO), Saint Petersburg, Russian Federation, pp. 1-1, (2022).
