CC BY
36
abstracts. phytopharm 2012
THE STUDY OF THE STABILITY OF THE GEL FORMULA AND THE ABSORPTION IN THE HAIR OF BLUE PEA EXTRACT GEL AND CRC EXTRACT GEL
© Chaipradit K.1, Priprem A. 2, Nualkaew S.3, Preeprame S.--
'Borabeu hospital, Amphur borabeu, Mahasarakam Thailand 2Faculty of pharmaceutical sciences, Khon Kaen University, Thailand 3Faculty of pharmaceutical sciences, Mahasarakam University, Thailand
The major objective of this study was the stability blue pea extract gel and CRC extract gel and the adsorption distance of the gel in the gray hair. Blue pea extract was prepared by extraction of the petal of blue pea (Clitoria ternatea L.) in hot water for 2 hours and evaporate to 1 gram per 1 milliliter concentration. Blue pea extract gel was formulated with 32 % w/v blue pea extract, 2 % hydroxypropylmethylcellulose, 30 % of ethyl alcohol 0.5 % Bronidox 0.17 % triethanolamine and purify water to 100 ml. CRC extract was the combination of anthocyanin extract from rose and blue pea (C = Clitorea ternatea L., R = Rosa hybrida L., C = complex). The CRC extract was in patent process and was obtained from associated professor Aroonsri Priprem, faculty of pharmaceutical sciences, Khon Kaen University Khon Kaen Thailand. CRC extract gel was formulated with 0.1% CRC extract 2.5 % hydroxypropylmethylcellulose, 60 % ethyl alcohol, 0.5 % Bronidox, 0.4 % triethanolamine and purify water to 100 ml. The gels formulated were tested the stability by evaluated the color, pH, viscosity and clarity after freeze and thaw condition for 3 cycles. The adsorption distances in the gray hair were evaluated by measuring distance of color absorption from out layer to inside of the hair within 5 minute after applying the gel to the hair. The percent of adsorption in the hair in 5 minute of blue pea extract gel, CRC extract gel, 0.1 % w/v lawsone in ethanol and benchmark product containing p-Phenylenediamine (PPD) were calculated and com-
paring the adsorptions character using microscope. The permanent of the staining were evaluated by apply the staining gel to the hair for 1 minute and leaved on 5 minutes, then measuring the adsorption distance in the hair, then washing the hair with baby shampoo and water for 1 minute, leave the hair dry for 5 minutes and observed the color and the absorption distance, repeated the washing step and observed the color and absorption change 4 times. After freeze and thaw condition the color of blue pea gel was not change, the color of CRC gel was change 2 color scale. The pH of Blue pea gel decrease 4.8 % and pH of CRC gel increase 0.9 %.The viscosity of blue pea extract gel was increased 3.4 % and viscosity of the CRC extracted gel was increased 1.6 %. The clarity of the gel was not change. The absorption distance of the CRC extract gel was more than Blue pea gel significantly. The CRC extract gel and the benchmark product contain PPD had none significantly absorption distance. The permanent of hair color after washing showed that the blue pea extracted gel could not remain hair color, the CRC extract gel and 1 % lawsone solution remain hair color for 2 time of washing, the benchmark product contain PPD remain hair color for 4 time of washing. The further development of CRC extracted gel for hair staining should focus on improvement of the permanent staining property which could promoted this substances to be an alternative natural and non toxic hair staining in the market.
EFFECT OF CRUDE EXTRACTS OF PHYLLANTHUS
NIRURI L. TOWARDS PLATELET AGGREGATION EVALUATED IN
96-WELLS MICROPLATE READER
© Puspita N. A., Pye D.
Centre for Biochemistry, Drug Design and Cancer Research, University of Salford, U. K. M4 5WT
Phyllanthus niruri L. is one of tropical plants that have been widely used in many countries as a traditional remedy for treating haemorrhagic diseases, particularly in the case of low platelet count and disturbance of platelet functions. However, literature discussing its pharmacological effects in platelet functions is very limited. The aim of this study is to explore the effect of crude Phyllantus niruri L. extracts towards inhibition of plate-
let aggregation. The plant is extracted with maceration method using various solvents; hexane, chloroform, ethanol, methanol, and water. Adenosine 5'-diphos-phate as an activating agent is used to induce platelet aggregation; the extent of inhibition was monitored in 96-wells microplate reader. The result (tab.) demonstrated that hexane, chloroform, ethanol, methanol, and water extracts of Phyllanthus niruri L. in concentration
ТОМ 10/2012/2 Obzory po kliniceskoj farmacologii i lekarstvennoj terapii [Reviews of clinical pharmacology and drug therapy] И ^^ 89
