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PRODUCTION OF ANTI-MADECASSOSIDE POLYCLONAL ANTIBODY AND ITS APPLICATION FOR QUALITY CONTROL OF CENTELLA ASIATICA (L.) URBAN
© PutalunWaraporn12, Tassanawat Patcharin 12, Yusakul Gorawit12, Sritularak Boonchoo3, Juengwatanatrakul Thaweesak4, Tanaka Hiroyuki5
1Faculty of Pharmaceutical Sciences, Khon Kaen University, Khon Kaen, 40002, Thailand. 2Research Group for Pharmaceutical Activities of Natural Products using Pharmaceutical Biotechnology (PANPB), National Research University-Khon Kaen University, Khon Kaen 40002, Thailand. 3Faculty of Pharmaceutical Sciences, Chulalongkorn University, Bangkok 10330, Thailand. 4Faculty of Pharmaceutical Sciences, Ubon Ratchathani University, Thailand. 5Graduate School of Pharmaceutical Sciences, Kyushu University, Fukuoka 812-8582, Japan
Table. Platelet Aggregation inhibition
Adenosine Water Extract Hexane Extract Chloroform Extract Ethanol Extract Methanol Extract
Inhibition ( %) 68.6 ± 4.3 72.7 ± 3.3 40.1 ± 5.6 44.7 ± 2.7 43.5 ± 6.6 68.8 ± 6.7
Values are mean ± SE (n = 8). Platelet counts was 1.5x108 cells/mL
of 250mg/ml showed a potent inhibition effect toward platelet aggregation. Compared with other extracts, water extract exhibited the strongest inhibition as well as adenosine (50uM/ml) as a positive control.
in Addition to this, methanol extracts showed relatively similar inhibition pattern with adenosine. Finally, this initial finding has provided the evidence of Phyllan-tus niruri L. action in platelet functions.
Madecassoside is the major triterpenoid saponins derived from Centella asiatica (L.) Urban, a medicinal plant used in Ayurvedic medicine for centuries. Madecassoside possesses various biological activities, including antidepressant effect (1), burn wound healing and anti-inflammatory (2). in this study, we developed the immunoassay method for quantitative analysis of the major compounds in C. asiatica. A sensitive indirect competitive enzyme-linked immunosorbent assay (ELiSA) was developed using polyclonal antibody against madecassoside. Madecassoside-bovine serum albumin conjugate was immunized to rabbits for producing polyclonal antibody. The results showed that the antibodies were specific for madecassoside and aisaticoside. The
range of the assay extends from 0.39 to 50 ig/ml. A good correlation between ELiSA and HPLC methods was obtained when analysis of total triterpenoid saponins in the crude extracts of plant samples. in addition, the products containing C. asiatica in various preparations were determined triterpenoid saponins content by competitive ELiSA. The developed ELiSA method can be used for quality assessment of C. asiatica and their products. References: (1) Liu, M. R., Han, T., Chen, Y., Qin, L. P., Zheng, H. C. and Rui, Y. C. (2004). Journal of Chinese integrative Medicine, 2 (6), 440-444. (2) Li, H., Gong, X., Zhang, L., Zhang, Z., Luo, F., and Zhou, Q. (2009). Phytomedicine, 16 (6-7), 538-546.
METABOLIC EFFECTS OF CITRUS GRANDIS WHOLE FRUITS EXTRACT IN THE STREPTOZOTOCIN-INDUCED DIABETIC RATS
© Raasmaja Atso1, Kovaleva Maria A.2, Makarova Marina N.2, LiXiang Ming3, Zou Jianqiang3, Zhu Guo-Guang3, Pozharitskaya Olga N.2, Makarov Valery G.2, Shikov Alexander N.2, Hiltunen Raimo 4
1Division of Pharmacology & Toxicology, Faculty of Pharmacy, University of Helsinki, P. O. Box 56 (Viikinkaari 5E), FIN-00014 Finland. 2St-Petersburg Institute of Pharmacy, 47/5, Piskarevsky pr., 195067, St.-Petersburg, Russia. 3Guangdong Huazhou Green Life Co., Ltd., Huazhou City, Guangdong, PRC. 4Division of Pharmaceutical Biology, Faculty of Pharmacy, University of Helsinki, P. O. Box 56 (Viikinkaari 5E), FIN-00014 Finland
Citrus grandis fruits are used as a folk remedy in medicine for treatment of hypertension, in Chinese Korea for hangovers, peel of the fruit in Taiwan for the medicine as antitussive, expectorant and antiasth-treatment of stomach ache, in Cameroon traditional matic. Dried Citrus peels, known as Chenpi are used in
90 ® Obzory po kliniceskoj farmacologii i lekarstvennoj terapii [Reviews of clinical pharmacology and drug therapy]
TOM 10/2012/2
east traditional medicine. it is reported to be popular in the diabetes patients with exuberant heat due to yin deficiency. Health beneficial effects of the dried extract of whole fruits of C. grandis (L.) Osbeck var. tomentosa hort. containing 19 % naringin were studied in neonatal streptozotocin (nSTZ)-induced NiDDM rats. The effects of citrus extract and the reference compound sitagliptin were followed on the pancreas histology and on the serum lipid, glucose and insulin levels. The nSTZ treatment led to increased glucose and decreased insulin levels in parallel with marked
pancreatic damage. These effects were not observed at least in similar manner in the nSTZ rats given also the citrus extract for several weeks. Beneficial effects of the citrus extract were found on the insulin, glucose and lipid levels as well as on improved pancreatic morphology. The promoting effects seemed to be dependent on an optimal dose since in some measurements they were found especially at certain extract amounts. it is suggested that the treatment of nSTZ diabetic rats with the citrus extract is associated with specific metabolic improvements.
GASTROPROTECTIVE EFFECT OF FENNEL
"FOENICULUM VULGARE" A COMMONLY USED SPICE IN ARAB
TRADITIONAL MEDICINE
© Rafatullah Syed1, Alqasoumi Saleh 1, Al-Dosari Mohammed 1, Al-Said Mansour1, Al-Yahya Mohammed1, Al-Mofleh Ibrahim2
Medicinal, Aromatic and Poisonous Plants Research Center (MAPPRC), College of Pharmacy, P. O. Box 2457, King Saud University, Riyadh 11451, Saudi Arabia, Email: [email protected]
2Department of Medicine, Gastroenterology Unit, College of Medicine, King Khalid University Hospital, P. O. Box 2925, Riyadh-11461, Saudi Arabia
Spices are a group of esoteric food adjuncts that have been used for thousands of years to enhance the sensory quality of foods and as preservatives. Much health benefit attributes of these common food adjuncts in animal studies include digestive stimulant action, antioxidant, anti-inflammatory and antihepoto-toxic properties. Among these, the gastronomic and antioxidant properties of a few specific spices have far-reaching neutraceutical value. The present study was carried out to evaluate antiulcerogenic property of Fennel "Foeniculum vulgare" Mill. (Fam.: Umbel-liferae) in Wistar albino rats. An aqueous suspension of fennel (a common dosage form used in folk medicine practice). The suspension was used in two doses (250 and 500 mg/kg body weight, orally) in all experiments except in Shay rat model. Gastric acid secretion studies were undertaken using pylorus ligated (Shay) rats. Gastric lesions in the rats were induced by noxious chemicals including ethanol, strong alkalis and in-domethacin. The levels of gastric wall mucus (GWM), nonprotein sulfhydryls (NP-SH) and malondialdehyde (MDA) were also measured in the glandular stomach
of rats following ethanol administration. The gastric tissue was also examined histologically. in pylorus-li-gated Shay rats, the suspension of fennel significantly reduced the basal gastric acid secretion, titratable acid and ruminal ulceration (64 °%, 39 % and 100 %>), respectively. The suspension significantly (P < 0.001, P < 0.01 and P < 0.01) attenuated gastric ulceration induced by necrotizing agents (80 % ethanol, 0.2 mol/L NaOH, 25 % NaCl) respectively and indomethacin was found to be (P < 0.01). The cytoprotective and antiulcer effect was further confirmed histologically. Furthermore, the suspension significantly replenished the ethanol-induced depleted levels of GWM (P < 0.001), NP-SH (P < 0.05) and diminished (P < 0.01) (MDA) concentration of the rats' stomach. The data obtained confirmed the use of fennel suspension in various gastric ailments including stomach ulcers. Fennel reported to contains bio-active phytochemical substances, which might increase endogenous prostaglandins and mucus synthesis and exert its gastroprotective activity through its antioxidant, antisecretory and cytoprotective properties in rats.
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