CC BY
25
СООБЩЕНИЯ
УДК 582.29 (571.6)
E.A. Davydov1 S.I. Tchabanenko2 T.V. Makryi3 V.A. Khanin4
Е.А. Давыдов С.И. Чабаненко Т.В. Макрый В.А. Ханин
the second confirmed record
of UMBILICARIA PULVINARIA (LicHENizED ASTOMYcoTA)
since ITS original description IN 1914
ВТОРАЯ ПОДТВЕРЖДЕННАЯ НАХОДКА ЛИШАЙНИКА иМВШСАША РиЬУШАПА СО ВРЕМЕНИ ОПИСАНИЯ ВИДА В 1914 ГОДУ
Summary. Umbilicaria pulvinaria has been re-discovered for the Far East, the new collection from North Sakhalin being only the second confirmed record of the species. Its distribution is revised, the species being recognized as endemic to Sakhalin and Kamchatka. Literature reports of the species from Siberia are considered doubtful. Gyro-phoric and lecanoric acids were revealed by HPLC. Lecanoric acid is reported for the species for the first time.
Key words: Far East, Sakhalin, rare species, endemic, floristic finding, Umbilicariaceae.
Аннотация. Umbilicaria pulvinaria приводится с территории Северного Сахалина - второго (после locus classicus) местонахождения вида. Указания вида для Сибири признаны ошибочными. Посредством HPLC выявлены вторичные метаболиты: гирофоровая и леканоровая кислоты; леканоровая кислота приводится для вида впервые.
Ключевые слова: Дальний Восток, Сахалин, редкий вид, эндемик, флористическая находка, Umbilicariaceae.
Umbilicaria pulvinaria (Savicz) Frey is a very rare species, and the data on its geography and biochemistry are extremely scarce. It was described by Savicz (1914) based on his own gathering from South Kamchatka in 1908. During the subsequent seventy years, the species was known only from this single collection, but during the last three decades it was reported for Yakutia (Golubkova & Savicz, 1978), East Sayan (Sedelnikova, 2001) and Stanovoye upland (Makryi, 2002). Despite a long history of lichenological explorations of the Far East (Tchabanenko et al., 2002; Himel-brant & Kuznetsova, 2006) and extensive col-
lections made during Kamchatian expeditions in 2002-2010 by D.E. Himelbrant, E.S. Kuznetsova and I.S. Stepanchikova, U. pulvinaria has not been reported from the Far East since Savicz (1914) and is thus considered as an extremely rare species (Davydov et al., 2011). However, this species was revealed during a revision of the Umbilicariaceae collection from Sakhalin. To specify its distribution area, available specimens ever identified as U. pulvinaria were revised. Although Golubkova & Schapiro (1979) examined the secondary metabolite composition of U. pulvinaria by thin layer chromatography (TLC) and detected gyrophoric acid and
OOWOCC><X>OC<X>C<>C<X>C<X!C>OC<X>C<>C<^OC<X>C<>WOC<><X>OC<X>C<>C<^OC<X>C^^
'-Алтайский государственный университет, пр-т Ленина, 61; 656049, Барнаул, Россия; e-mail: eadavydov@yandex.ru 2Сахалинский ботанический сад, ул. Горького,21; 693013, Южно-Сахалинск, Россия
3Центральный сибирский ботанический сад СО РАН, ул. Золотодолинская, 101; 630090, Новосибирск, Россия 4Ботанический институт им. В.Л. Комарова РАН, ул. Проф. Попова, 2; 197376, С.-Петербург, Россия 1Altai State University, Lenina st., 61; 656049, Barnaul, Russia 2Sakhalin Botanical Garden, Gorky st., 21; 693013, Yuzhno-Sakhalinsk, Russia
3Central Siberian Botanical Garden, Siberian Branch of Russian Academy of Sciences, Zolotodolinskaya st., 101; 630090, Novosibirsk, Russia
4Russian Academy of Sciences, Komarov Botanical Institute, Prof. Popova, 2; 197376, St.-Petersburg, Russia
Поступило в редакцию 18.05.2011 г.
Submitted 18.05.2011
not-identified substance, the opportunity was taken to refine the chemical composition of the species.
Specimens, including those from the herbaria CANL, LE, NS, O and SAKH, were examined using standard light microscopy and secondary metabolites were determined using high-performance liquid chromatography (HPLC) based, with modifications, on the methodology of Feige et al. (1993). An Agilent 1200 HPLC system equipped with a gradient binary pump, micro vacuum degasser, standard autosampler, column compartment with thermostat and diode array, and multiple wavelengths UV-detector was used. A ZORBAX Eclipse XDB-C18 column (Agilent Technologies, USA), 5 pm, 4.6 x 150 mm at 26°C was applied. Solvent system A was 1% orthophosphoric acid and solvent system B was 100% methanol (high purity). The run started with 30% B at a flow-rate of 0.7 ml/min. After 5 pl of the extract was automatically injected, solvent system B was increased to 70% within 14 min, then up to 90% B within 30 min, then up to 100% B within 18 min and then isocratically in 100% B for further 20 min. At the end of the run, solvent system B was decreased to 30% within 1 min and the column flushed with 30% B for 16 min before a new run was started. The compounds were detected at ^=245 nm.
Umbilicaria pulvinaria (Saviez) Frey 1931, Hedwigia 71 : 114 - Gyrophora pulvinaria Savicz 1914, Bull. Jard Imp. Bot. Pierre Grand 15 : 117. Type: Kamchatka: In regionibus alpinis ad rupes montis “Krasnyi Jarczik” et montium “Pope-recznyje” prope pagum “Naczika”, 1908, leg. Savicz V.P. No. 5579 (LE-L 280!, lectotype designated by Wei in Wei & Jiang 1993 : 206).
Umbilicaria pulvinaria is distinguished by its polyphyllous cushion-like thallus attached by a thick black underthallus, and apothecia of actino-disc-type lacking the disc margin and with very thin
Fig. 1. Umbilicaria pulvinaria (LE-L 281, isolec-totype). Actinodisc apothecia, scale = 0,5 mm
Fig. 2. Umbilicaria pulvinaria (LE-L 7943). A -upper surface of thallus; B - lower surface of thallus, scale = 1 cm
fissures (Fig. 1, 2). The species morphologically resembles U. angulata Tuck. var. compacta Krog and U. lambii Imshaug, but the apothecia type differentiates these species well. Apothecia of U. angulata var. compacta are a gyrodisc with a well developed margin, whereas U. lambii has leiodisc apothecia. Umbilicaria pulvinaria can also be confused with polyphyllous forms of U. cinereorufescens, but the latter possesses thalloconidia on black rhizinomorphs and has a thicker thallus. The actinodisc apothecia of U. pulvinaria resemble those of U. muehlenber-gii (Ach.) Tuck. and U. polyrrhiza (L.) Ach., but the prior species has a large and rigid monophyllous thallus with light underthalline filaments and the latter one is characterized by well-developed rhizino-morphs bearing multicellular thalloconidia.
Two HPLC chromatograms were obtained. As the studied compounds have very similar molar extinctions, the coefficients for the wave lengths, 214 nm, 257 nm and 270 nm (Huneck & Yoshimura, 1996) which are close to ours used for the chromatographic detection (245 nm), the peak areas can be considered to be directly proportional to the concentrations of the compounds in our extracts. As a result of the HPLC analyses (Fig. 3), lecanoric acid was revealed as a mi-
Fig. 3. Gradient HPLC of the acetone fraction from Umbilicariapulvinaria (LE-L 280, lectotype). GYR - gy-rophoric acid, LEC - lecanoric acid.
nor compound and gyrophoric acid as the major in both the lectotype and the specimen from Sakhalin.
Umbilicaria pulvinaria was reported for Yakutia by Golubkova & Savicz (1978). However, voucher specimens identified by Savicz clearly belong to Lasallia caroliniana (Tuck.) Davydov, Persoh & Rambold. Last species may develop similar cushion-like growth form, but differs by a complex of characters which place it in another genus, i.e. Lasallia Merat (Davydov et al., 2010). Thus, reports of Umbilicaria pulvinaria are considered as doubtful for Yakutia. Specimens form Stanovoye upland (East Siberia) reported by Makryi (2002a, b) were identified as U. cinereorufescens (Schaer.) Frey. Unfortunately, no herbarim material from East Sayan was available for this study.
Revision of available material recognized U. pulvinaria to be a rare species restricted in its distribution to Kamchatka and Sakhalin. There are very few epilithic species of lichens confined to East Asia, e.g. Leptotrema lithophylla Oxner, Stereocau-lon saviczii DR., S. wrightii Tuck. and Ramalina rjabuschinskii Savicz (Chabanenko, 2008; Domb-rovskaya, 1996; Mikulin, 1990; Oxner, 1929, 1960; Tchabanenko et al., 2002; unpublished data of Tcha-banenko). An East Asian distribution is also characteristic of some mountain mosses, e.g. Bucklandiella vulcanicola (Frisvoll & Deguchi) Bednarek-Ochy-ra & Ochyra (Czernyadjeva, 2007; Czernyadjeva et Ignatova, 2008; Frisvoll, 1988). However, it is remarkable that all these species are more widely distributed than U. pulvinaria, occurring in addition to Kamchatka and Sakhalin, in the mountains of Sikhote-
Alin, in Hokkaido, Honshu and Kuril Islands. These regions therefore appear to be plausible localities for further records of U. pulvinaria.
Specimens examined: Kamchatka: In re-gionibus alpinis ad rupes montis “Krasnyi Jarczik” et montium “Poperecznyje” prope pagum “Na-czika”, 1908, leg. V.P. Savicz No. 5579 (LE-L 280, lectotype); [Southern] Kamchatka, [Elizovsky District], northern slope of Krasny Jarchik Mountain between Koriaki [Koryatskii Ostrog] and Nachi-ki, (same locality as the lectotype), VII 1908, leg. V.P. Savicz No. 5579 (LE-L 281, isolectotype), [Southern] Kamchatka, [Elizovsky District], Poperechny Range between Koriaki [Koryatskii Ostrog] and Nachiki, 1908, leg. V.P. Savicz (LE-L 282, syn-type); Sakhalin, Schmidt Peninsula, Cape Elizabeth, 54°25,110’ N 142°42,804’ E, alt. 300-400 m. Stones and boulder outcrops in the NW range; associated with Umbilicaria muehlenbergii, U. torrefacta, Lasallia caroliniana and species of Lecidea, Acarospo-ra, Porpidia, Rhizocarpon & Ophioparma. 15 VIII 2005, leg. S I. Tchabanenko (LE-L7943).
Misidentifications: Russia, Yakutia, Tom-ponsy region, Verkhoyanskyye Gogy Mt. upper part of a slope, alt. 1633.1 [m]. Fragments of grass-fruticulose-lichen tundra amidst stone fields, 1 VIII 1954, leg. V. Kuvaev 67/22 (LE-L6741) = Lasallia caroliniana; same region, East Verkhoyanye [upper part of Yana River Basin], Mola River Basin, right bank of Mudukyn River near its junction with the Udultyn River. Stony foothill of the mountain, 1954, leg. L.A. Dobretsova (LE-L7912) = L. caroliniana. Stanovoye upland, Kodar range, near headwaters
of the Apsat River, above timberline, [c. 57°23’N, 118°06’E] alt. 1700 m., stone outcrops, on boulder, leg. V.M. Burkova, 11 VII 1964 (NS) = U. cinere-orufescens; same upland, Udocan range, near headwaters of the Katugin River, [c. 56°41’N, 118°57’E], alt. 1950 m., stony tundra sand rock outcrops, on rocks, leg. V.M. Burkova, 24 VII 1966 (NS) = U. ci-nereorufescens.
Material of U. angulata var. compacta examined: Eastern Pacific Coast District, Juneau: Granite Basin, on rocks, alt. 3000 ft. [c. 950 m], 26 VIII 1957, leg. H. Krog No. 5203 (0-001014, holotype!).
Selected material of U. lambii examined: Canada, British Columbia, Mt. Assiniboine Prov. Park, Sunburst Lake, 50°55 N, 115°39 W, alt. 2400 m, 3 VIII 1951, I.M. Lamb 6584 (CANL 66543, holo-
type!); same province, Range 2 Coast Land Distr. 25 km SSW of Tatla, northern flank of Razorback Mt. 51.7 N, 124.75 W, 21 VII 1981, T. Goward, G. Leroux & R. Thomson 81-1490 (CANL 110846); same province, Kamloops Division Yale Land Distr., southern slopes of Trophy Mountains, 10 km south of Wells Gray Provincial Park, 52°00 N, 120°00 W, alt. 2350 m, 22 VIII 1984, T. Goward 84-970 (CANL 93955).
We gratefully acknowledge help from the curators and staff of cited herbaria, to Dr. I.V. Cz-ernyadjeva for her comments on moss distributions and to Professor Mark Seaward for improving the text. The study by E. D. was financially supported by the Russian Foundation for Basic Research (RFBR grant No. 11-04-90704).
literature
Chabanenko S.I. Rare lichens of protected natural territories of the Sakhalin region // Proceedings of the International conference “Actual problems of the botany in Armenia”. - Erevan, 2008. - P. 237-240.
Czernyadjeva I. V. Bucklandiella vulcanicola (Frisvoll & Deguchi) Bednarek-Ochyra & Ochyra / New national and regional bryophyte records, 16 // Journal of Bryology, 2007. - Vol. 29. - P. 198-199.
Czernyadjeva I. V., Ignatova E.A. Mosses of the Bystrinsky Nature Park (Kamchatka Peninsula, Russian far East) // Arctoa, 2008. - Vol. 17. - P. 49-62.
Davydov E.A., HimelbrantD.E., Stepanchikova I.S. Contribution to the study of Umbilicariaceae (lichenized Ascomycota) in Russia. II. Kamchatka Peninsula // Herzogia, 2011. - Vol. 24 (in press).
Davydov E.A., Persoh D. & Rambold G. The systematic position of Lasallia caroliniana (Tuck.) Davydov, Persoh & Rambold comb. nova and considerations on the generic concept of Lasallia (Umbilicariaceae, Ascomycota) // Mycol. Progress, 2010. - Vol. 9. - P. 261-266. DOI 10.1007/s11557-009-0636-y
Dombrovskaya A. V The genus Stereocaulon on the territory of former USSR. - St.-Petersburg, 1996. - 270 p.
Feige G.B., Lumbsch H.T., Huneck S., Elix J.A. Identification of lichen substances by a standardized highperformance liquid chromatographic method // Journal of Chromatography, 1993. - Vol. 646. - P. 417-427.
Frisvoll A.A. A taxonomic revision of the Racomitrium heterostichum group (Bryophyta, Grimmiales) in N. and C. America, N. Africa, Europe and Asia // Gunneria, 1988. - Vol. 59. - 289 p
Golubkova N.S., Savicz V.P. Familia Umbilicariaceae // Handbook of the lichens of the USSR, Vol. 5. - Leningrad, 1978. - P. 89-136.
Golubkova N.S., Schapiro I.A. Species generis Umbilicaria Hoffm. em. Frey in URSS cresentes et examinatio earum chemotaxonomica // Novitates Systematicae Plantarum non Vascularium (Academia Scientiarum URSS, Insti-tutum Botanicum Nomine V.L. Komarovii), 1979. - Vol. 16. - P. 133-153.
Himelbrant D.E., Kuznetsova E.S. Lichens of Kamchatka - history of investigations and recent data // Lichen flora of Russia: state and perspective of exploration. Proceedings of the international conference dedicated to the 120th anniversary of V.P. Savicz, 2006. - P. 66-75.
Huneck S., Yoshimura I. Identification of lichen substances. - Berlin: Springer, 1996. - 493 p.
Makryi T. To the lichen flora of the Stanovoye nagorye uplands (Baikalian Siberia). I. The epilithic lichens of the Kodar range // Turczaninowia, 2002a. - Vol. 1, № 5. - P. 47-67.
Makryi T. V. Umbilicaria pulvinaria (Savicz) Frey. // Red data book of the Chitinskaya Oblast’ and Aginsky Buriatsky Autonomny Okrug. Plants. - Chita, 2002b. - P. 236.
Mikulin A.G. Handbook of the lichens of Kamchatka Peninsula. - Vladivostok, 1990. - 128 p.
Oxner A.N. Über Ramalina Rjabuschinskii Sav. // Bulletin du Jardin Botanique de Kieff, 1929. - Vol. 9. -P. 82-86.
Oxner A.N. A new species of lichen Leptotrema lithophila sp. n. in the flora of the U.S.S.R. // Ukrainskii Bot. Zhurn., 1960. - Vol. 17, № 4. - Pp. 67-74.
Savicz V.P. Neue Flechten aus Kamtschatka // Bull. Jard. Imp. Bot. Pierre le Grand, 1914. - Vol. 14. - P. 111-128.
Sedelnikova N. V. Lichens of West and East Sayan. - Novosibirsk, 2001. - 190 p.
Tchabanenko S.I., Skirina I.F., Knyazheva L.A. Checklist of the lichens and lichenicolous fungi of Primorsky krai. - Yuzhno-Sakhalinsk, 2002. - 89 p.
