CC BY
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STUDY OF PROPERTIES OF WHEAT GERMINS AND MEALS AND THEIR USE IN THE PRODUCTION OF DIETARY HARDTACKS
Pavlo Pyvovarov1, Tetiana Cheremskaya2, Maryna Kolesnikova3, Svitlana Iurchenko4, Svitlana Andrieieva5
department of Food Technology in the Restaurant Industry, Kharkiv State University ORCID: https://orcid.org/0000-0001-9119-12252
2Department of Food Technology in the Restaurant Industry, Kharkiv State University ORCID: https://orcid.org/0000-0001-6518-3889
3Department of Food Technology in the Restaurant Industry, Kharkiv State University ORCID: https://orcid.org/0000-0002-6223-7105
4Department of Food Technology in the Restaurant Industry, Kharkiv State University ORCID: https://orcid.org/0000-0003-1286-081X
department of Food Technology in the Restaurant Industry, Kharkiv State University ORCID: https://orcid.org/0000-0003-2981-481X
^Corresponding author: Svitlana Andrieieva, e-mail: [email protected]
ABSTRACT
Object of research: technology of production of dietetic hardtacks using germs and meal of wheat germ, by defatting them.
Investigated problem: the process of defatting wheat germ meal not only reduces the total amount of lipids, but also contributes to a change in the ratio of individual fatty acids. Main scientific results: in order to use wheat germ meal as the main raw material, together with wheat germ in the production of dietetic hardtacks, their amino acid composition, biological value and balance of essential amino acids were studied in comparison with the FAO/ WHO standard. The mineral, vitamin and fatty acid composition of the germ and meal of wheat germ has been determined. The results obtained were used in the development of the recipe composition and the technological process for the production of dietetic hardtacks. The results obtained (calculation of the balance according to the "tryptophan" and "threonine" indices) confirm that wheat germ meal is a valuable raw material that can be used in technologies for the production of high-protein food products.
The moisture-absorbing capacity of wheat germ and meal was studied in order to determine their ability to form dough. It has been established that the best performance is characterized by a mixture of wheat germ meal and wheat germ in a ratio of 80:20. The ability of digestion by enzymes of the gastrointestinal tract "in vitro" of wheat germ meal proteins has been investigated, which determines the biological value of food products and makes it possible to predict the degree of their utilization by the body. The field of practical use of the research results: production of dietetic hardtacks in restaurant enterprises, in specialized confectionery shops, as well as within the boundaries of food production
An innovative technological product: the possibility of using valuable secondary raw materials in food production technology - wheat germ and meal, which is a source of high-quality protein, vitamins and minerals. This will make it possible to adjust the nutritional and biological value of flour confectionery products, as well as improve the nutritional structure of consumers of these products.
Scope of application of an innovative technological product: dietetic hardtacks.
© The Author(s) 2021. This is an open access article under the Creative Commons CC BY license
1. Introduction
1. 1. The object of research
Development of technology for dietetic hardtacks using wheat germ and meal.
1. 2. Problem description
It is known that nutrition is one of the most important factors determining the health status of the population. Proper nutrition ensures normal growth and development, contributes to the pre-
of Food Technology and Trade, Kharkiv, Ukraine of Food Technology and Trade, Kharkiv, Ukraine of Food Technology and Trade, Kharkiv, Ukraine of Food Technology and Trade, Kharkiv, Ukraine of Food Technology and Trade, Kharkiv, Ukraine
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Article history: Received date 15.07.2021 Accepted date 26.08.2021 Published date 31.08.2021
Section:
Food production
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DOI
10.21303/2313-8416.2021.002039
KEYWORDS
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wheat germ wheat germ meal hardtacks nutritional value biological value
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vention of human diseases, and affects the quality of life. It should be noted that in recent years, the structure and chemical composition of the diet of the population has changed significantly, which is associated with the deterioration of their financial and economic condition. Therefore, the urgent issue is the problem of adjusting the structure of nutrition.
The food industry is actively developing: new sources of raw materials are used, as well as technologies that make it possible to process agricultural raw materials without losing valuable components. These are the processes of development and implementation of innovations that make it possible to provide the population with high-quality food products that can compensate for nutrient deficiencies and become a source of necessary regulators of all functions of organs and systems of the human body.
Taking into account the indicated problems, in this work the use of wheat germ meal obtained as a result of cold pressing of oil, together with wheat germ in the production of dietetic hardtacks, is substantiated.
1. 3. Suggested solutions to the problem
Hardtacks are flour products that resemble lingering cookies, made from wheat flour and water with or without other raw materials, which satisfy hunger well and are suitable for daily consumption, including instead of bread.
Wheat germ is a unique natural concentrate that contains (in % on dry matter): proteins 33.. .45, sugars 21.. .30, lipids 13... 19, minerals 4.. .7, fats 8.. .17, fiber 2.. .3, vitamins, enzymes.
It has been established that wheat germ contains 21 macro- and microelements, including calcium, phosphorus, iron, as well as magnesium, potassium, iodine, selenium, zinc, etc. The vitamin composition of wheat germ is also unique, which is practically a multivitamin concentrate. In terms of tocopherol content (12...33 mg %), wheat germ exceeds most food products. 12 vitamins have been identified, in particular, tocopherols, thiamine, provitamin A (carotene), niacin (PP), riboflavin (B2), ergocalciferol (D2), pyridoxine (B6), pantothenic acid (B3), folic acid [1-3].
The embryo contains phytohormones (auxin, heteroauxin, etc.), it is an energy base for living cells and the production of immunocytes in the body, which reduce the toxic effect of harmful factors on the human body. In addition, wheat germ is a promising phytoenterosorbent. As a result of the consumption of wheat germ, an increase in the content of proteins in the blood was noted, there is a normalization of metabolic processes in the liver and throughout the body, an improvement in blood composition and the function of the thyroid and pancreas [4-6].
Wheat germ meal is a valuable secondary raw material, which is a source of high-quality protein, the content of which is about 43 %, carbohydrates - 47 %, fiber - 13.2 %, vitamins of group B, PP, D 2, carotene, tocopherol, thiamine, folic acid. It also contains macro - and microelements calcium, phosphorus, iron, as well as magnesium, potassium, iodine, selenium, zinc, etc. [7-9].
It can be concluded that the use of germs and meal of wheat germ in the recipe composition of dietetic hardtacks is a promising direction in the production of health food products. However, despite the high nutritional and biological value, the technological properties of wheat germ meal are rather low. In this regard, it is necessary to search for effective ways of processing wheat germ meal in order to use it in the technology of health food products.
2. Materials and methods
The protein content in the raw material was determined by the Lowry method modified by Miller [10].
The lipid content was determined by the gravimetric method according to E. Bligh and W. Dyer [11].
The fatty acid composition of lipids was determined using gas chromatography of methyl esters of fatty acids [12]. The studies were carried out on a GC-14BPF gas chromatograph with the following parameters: the injector temperature was 200 °C, the detector temperature was 200 °C, the evaporator temperature was 180 °C, and the carrier gas was argon. Calculation of the amount of free fatty acids [12, 13] was carried out using the calibration graph of a standard mixture of fatty acids.
The content of vitamins was determined according to the methods [13].
The mineral composition was determined on a PAZh-3 flame spectrophotometer with appropriate light filters [14].
The amino acid score of raw proteins and the degree of amino acid balance were determined by the method proposed by FAO/WHO [14].
Enzymatic hydrolysis of proteins in vitro was determined by the method of A. Pokrovsky and E. Ertanov [15]. Let's use the enzymes pepsin, trypsin, and chymotrypsin produced by Bio-pharma (Kyiv, Ukraine). Enzymatic hydrolysis of proteins was determined under conditions of sequential action of pepsin at pH 1.2 and /=37 °C and a mixture of trypsin and chymotrypsin at pH 8.2 and /=37 °C. In the products of enzymolysis, the degree of protein degradation was calculated by the Lowry color reaction and expressed in arbitrary units (^g of tyrosine per 1 mg of protein).
3. Results
The chemical composition, as well as the functional and technological properties of wheat germ meal depends on many factors that make up the technological background of the processed raw materials, methods and conditions for its production, etc. [16, 17]. The influence of the defat-ting process on the chemical composition of the wheat germ meal is presented in Table 1.
Studies have shown that wheat germ is characterized by variability in the content of lipids and poliosis. The content of nitrogen-containing components is characterized by relative stability in terms of quantitative indicators and amino acid composition (Table 2).
Table 1
Chemical composition of wheat germ and meal, %
. Moisture Lipid Content of protein and nitro- Carbohydrate content Ash Unidentified
content content gen-containing substances ~hexoses polyoses content fractions
Wheat germ 4.2±0.01 12.3±0.02 39.0±0.3 3.6±0.03 37.5±0.3 0.7±0.01 2.7±0.02
Wheat germ meal 3.9±0.03 1.1±0.05 42.5±0.5 4.1±0.01 43.7±0.5 0.9±0.02 3.9±0.03
Table 2
Amino acid composition of wheat germ and meal
Sample
Amino acid name Wheat germ Wheat germ meal
Amount of ami- Amino acid con- Amount of amino Amino acid con-
no acids, mg % tent, % acids, mg % tent, %
Essential amino acids, including: 15,901.3 40.8 17,724.9 41.7
valine 1,950.1 5.0 2,857.0 6.7
methionine 1,182.8 3.0 1,369.9 3.2
leucine+isoleucine 5,560.8 14.3 5,452.9 12.8
lysine+histidine 1,971.1 5.1 2,280.1 5.4
threonine 1,439.3 3.6 1,866.6 4.4
tryptophan 419.7 1.1 430.9 1.0
phenylalanine 3,377.5 8.7 3,467.5 8.2
Essential amino acids, including: 23,102.5 59.2 24,727.2 58.3
alanine 5,011.4 12.8 5,144.9 12.1
arginine 1,736.7 4.5 1,782.3 4.2
aspartic acid 2,216.6 5.6 2,780.3 6.5
glutamic acid 1,384.2 3.6 1,421.1 3.4
proline 5,154.3 13.2 5,544.2 13.1
serine 4,465.4 11.5 4,837.0 11.4
tyrosine 2,230.1 5.7 2,289.6 5.4
cystine 903.8 2.3 927.8 2.2
Total amino acids 39,003.8 100.00 42,452.1 100.00
These data indicate that defatting does not entail a significant change in the amino acid composition of the embryos, but leads to a relative increase in the content of the total number of amino acids from 39.0±0.3 % to 42.5±0.5 %. The share of essential amino acids is also increasing, the increase is almost 1.8 %.
The biological value of wheat germ and wheat germ meal in comparison with the amino acid score of reference proteins is given in Table 3 [18], the balance according to "tryptophan" and "threonine" indices - in Table 4.
Table 3
The biological value of wheat germ and meal of wheat germ by amino acid score
FAO/WHO scale Wheat germ Wheat germ meal
Amino acid name mg/per gram of mg/per gram of amino acid mg/per gram amino acid
protein protein score of protein score
Leucine+Isoleucine 110 142.6 129.6 128.5 116.8
Ly sine+histidine 55 50.5 91.9 53.7 97.7
Valine 50 50.0 100.0 67.3 134.6
Tryptophan 10 10.8 108.0 10.6 108.0
Threonine 40 36.9 92.3 44.0 109.9
Phenylalanine+Ty ro sine 60 143.9 239.7 135.6 226.0
Methionine+cystine 35 53.5 152.9 54.1 154.6
From the Table 3 it is possible to see that the limiting values for wheat germ are lysine with histidine (score 91.9 %) and threonine (score 92.3 %). Probably, during defatting, a relative increase in the threonine content occurs due to the removal of fats, as a result of which lysine with histidine is limiting for proteins in wheat germ meal (score 97.7 %).
Table 4
Balance of essential amino acids of germ and wheat germ meal in comparison with the FAO/WHO standard
Balance of amino acids according to the Balance of amino acids according to the
«tryptophan» index «threonine» index Amino acid name -
FAO/WHO scale Wheat germ Wheat germ meal FAO/WHO scale Wheat germ Wheat germ meal
Threonine 2...3 3.4 4.4 1.0 1.0 1.0
Lysine+histidine 3...5 4.7 5.3 1.1 1.4 1.2
Valine 4 4.6 6.7 1.5 1.4 1.5
Leucine+Isoleucine 7.10 13.2 12.7 3.1 3.9 2.9
Phenylalanine 2.4 8.0 8.1 1.1 2.4 1.9
Methionine 2.4 2.8 3.2 0.7 0.8 0.7
Tryptophan 1,0 1.0 1.0 0.25 0.3 0.2
Balance calculations based on indices indicate that the proteins of wheat germ and wheat germ meal are overloaded with threonine (when calculating the balance using the "tryptophan" index), leucine, isoleucine and phenylalanine. The results obtained confirm that, taking into account the given data, wheat germ meal is a valuable raw material that can be used in technologies for the production of high-protein food products.
The mineral and vitamin composition of wheat germ and wheat germ meal is presented in Tables 5, 6.
The given data (Table 5) testifies that the degreasing process does not significantly affect the qualitative composition of mineral elements, relatively increasing their content in terms of 100 g of natural product. This is the result of the fact that minerals are practically not included in the lipid fractions.
The results obtained (Table 6) show that defatting significantly affects the vitamin composition of wheat germ. Along with fats, fat-soluble vitamins are removed during the extraction process, reducing their content in the meal. The content of water-soluble vitamins is also reduced: pyridoxine, nicotinic acid by 8 times, biotin, choline - by 6, thiamine - by 5 times, which may be the result of exposure to organic solvents or the selected defatting parameters.
Table 5
Mineral composition of wheat germ and wheat germ meal
Mineral composition, mg/ %
Name of mineral substances -
Wheat germ Wheat germ meal
Calcium (Ca2+) 29.0±0.2 37.0±0.2
Magnesium (Mg2+) 96.0±0.4 154.0±0.6
Potassium (K+) 252.0±0.8 312.0±0.8
Sodium (Na+) 5.0±0.03 8.0±0.04
Iron (Fe2+/3+) 20.6±0.1 7.5±0.04
Copper (Cu) 0.25±0.001 0.32±0.001
Chlorine (Cl-) 112.0±0.8 144.0±0.8
Phosphates 196.0±0.9 216.0±0.9
Zinc (Zn2+) 0.37±0.001 0.44±0.001
Manganese (Mn) 0.18±0.001 0.27±0.001
Cobalt (Co) 0.009±0.001 0.015±0.002
Nickel (Ni) 0.005±0.001 0.009±0.001
Chromium (Cr) 0.001±0.005 0.002±0.001
Total 711.0±1.0 879.0±1.0
Table 6
Vitamin composition of wheat germ and wheat germ meal
Name of vitamins
Biotin (vitamin H) Thiamin (vitamin B1) Riboflavin (vitamin B2) Pyridoxine (vitamin B6) Tocopherols (vitamin E) Niacin (vitamin PP, niacin) Carotenoids (provitamin A)
Folacin (folic acid) Pantothenic acid (Vitamin B3) Choline
Vitamin content, ^g/g
Wheat germ Wheat germ meal
0.86±0.01 0.14±0.001
51.2±0.3 10.3±0.05
15.5±0.2 4.5±0.03
152.8±0.8 19.3±0.1
164.6±0.8 0.137±0.001
359.9±0.9 47.2±0.3
56.7±0.3 1.0±0.05
3.8±0.03 0.97±0.01
34.7±0.1 2.0±0.02
939.0±1.0 148.0±0.9
In the process of defatting wheat germ, the total amount of lipids is significantly reduced (Table 7), which affects the nutritional value of raw materials.
Table 7
Fatty acid composition of lipids of wheat germ and wheat germ meal
Name of fatty acid, index
Fatty acid content, % of the total
Wheat germ
Wheat germ meal
Myristic, C140
Palmitic, C
3 16:0
Sterna C180 Arachidonic, C200 Palmitoleic, C
16:1
O^ C18:1
Linoleic, C182 a-Linolenic, C183 Not identified
The total amount of lipids in the samples, %
0.2±0.001 17.9±0.1 14.5±0.1 0.1±0.001 1.5±0.02 57.7±0.3 6.1±0.03 2.0±0.02
12.3±0.2
18.2±0.1 16.0±0.1 0.1±0.001
56.7±0.3 8.2±0.03
0.8±0.01 1.1±0.05
From the data presented (Table 7) it can be seen that defatting not only reduces the total amount of lipids, but also contributes to a change in the ratio of individual fatty acids in lipids. It is found that the meal of wheat germ is characterized by more stable physicochemical parameters in comparison with wheat germ (Table 8).
Table 8
Physicochemical parameters of wheat germ lipids
Index Value
Acid number, mg KOH/g 1.8±0.02
Peroxide number, 1/2 O mol/kg 1.7±0.02
Mass fraction of non-saponifiable substances, % 1.1±0.02
Mass fraction of tocopherols, mg % 175.0±0.9
Based on the studies carried out, it is advisable to use wheat germ meal as the main raw material, since its nutritional and biological value is not significantly inferior to wheat germ. In order to enrich hardtacks with lipids, wheat germs were additionally introduced into their recipe composition. On the basis of preliminary studies and analysis of organoleptic indicators, the optimal concentration of adding wheat germ to dietetic hardtacks in an amount of 20 % of the mass of dry prescription components has been established.
Considering the fact that the technological properties of wheat germ meal are rather low, it was important to determine the moisture absorption capacity (MAC) of meal, wheat germ and a mixture of meal and wheat germ (Fig. 1). The studies were carried out at a temperature of 20 °C.
MAC 0/58.5 58.0 57.5 57.0 56.5 56.0 55.5 55.0 54.5
1 2 3
Fig. 1. Moisture absorption capacity, %: 1 - a mixture of wheat germ meal and wheat germ (80:20); 2 - wheat germ; 3 - wheat germ meal
It has been established that the highest moisture absorption capacity is characteristic of a mixture of germs and wheat germ meal.
The influence of the defatting process on the ability of digestion by enzymes of the gastrointestinal tract "in vitro" of wheat germ meal proteins has been investigated. The rate of protein digestion in the gastrointestinal tract by proteolytic enzymes is one of the important indicators; therefore, the results of protein digestion in "in vitro" conditions can be used to predict the degree of their utilization by the body [19, 20].
From the obtained experimental data on the accumulation of products of enzymatic hydrolysis of protein (Fig. 2), it can be seen that at the stage of pepsinolysis, the depth of hydrolysis of wheat germ meal is 1.2 times higher than that of wheat germ.
The digestibility of wheat germ is 118.2 ^g/mg, a similar indicator of wheat germ meal is slightly higher - 148.2 ^g/mg, and a mixture of meal and wheat germ is 142.2 ^g/mg, which confirms their use in technology.
Taking into account analytical and experimental studies, a recipe composition and a technological scheme for the production of dietetic hardtacks using germs and wheat germ meal have been developed (Fig. 3).
Wheat Wheat germ Mixture of
germ meal wheat germ
meal and
| pepsinolysis | | trypsinolysis Fig. 2. Enzymatic hydrolysis of germ protein, wheat germ meal and their mixture by proteolytic enzymes
Wheat germ (^Wheat germ/^^~*
Prepared water, t=20°C
J}
Screening, d=1.5...2 mm
C
Screening, d=1,5...2 mm
"Wheat germ meal" semi-finished product
T
"Wheat germs" semifinished product
Quinoa J* sl. Amaranth Sesame C „Linen
Mixing, x=(4...5)X60 c
I
Withstanding, t=(28. . .30) X 60 c
T
"Dough for hardtacks" semi-finished product
Forming, h=1,5.. .2 mm 0=40.45 mm
ZE
____j
B
"Cookies hardtacks" semi-finished product
±
Baking,
t=105...n0°C, x=(47...50)x60 s
I
Cooling, t=20 °C
T-
Package
im
Storage, implementation
T
C
Hardtacks
Fig. 3. Technological scheme for the production of dietetic hardtacks using wheat germs and
wheat germ meal
4. Discussion
In the course of research, it has been determined that the process of defatting wheat germ for hardtacks does not lead to a change in the total amino acid composition of wheat germ, but the proportion of essential amino acids increases. Also, scientists [21] have developed the technology of butter cookies for patients with diabetes mellitus using wheat germ. The technological process for the production of hardtacks implies pre-roasting of wheat germ in order to reduce the characteristic bean flavor, however, heat treatment has reduced the composition of essential amino acids.
In the course of studies of enzymatic hydrolysis, it has been determined that a mixture of meal and wheat germ can be digested by enzymes of the gastrointestinal tract. Similar studies were also carried out by scientists [22], which confirm the presence of a harmonious enzyme system (lipase, lipoxygenase, catalase) in wheat germ. The presence of these enzymes affects their quality indicators during storage and thereby complicates their industrial use on a large scale.
Based on the results of physicochemical studies, it has been determined that the highest moisture absorption capacity of 58.3 % is characterized by a mixture of germs and wheat germ meal. In [23], a technology was developed for obtaining cake from wheat germ, the moisture absorption properties of which depended on the degree of dispersion of the cake. So, the authors determined that during grinding, the surface area of the particles increases, which contributes to an increase in the amount of adsorbed-bound moisture. When grinding wheat germ cake to an average particle size of 0.12 mm, the moisture absorption capacity increases to 78.3 %, which provides a homogeneous and monolithic structure in the finished product. However, the authors of [24] confirmed the opposite, that a finer grinding of wheat germ (particle size 0.8 ... 0.12 mm) significantly impairs the organoleptic characteristics of the product.
To obtain a finely dispersed system of wheat germ meal mixtures, technological equipment are necessary that will grind raw materials with certain dimensional characteristics. To date, grinding is carried out by the method of dry mechanical activation, however, in the process of mechanical activation, significant destruction occurs, as well as the formation of various integral violations of the feedstock. Therefore, the selection of technological equipment, as well as the determination of the degree of dispersion, using the methods of modern photo-microscopy, is one of the main limitations of the study.
Thus, the prospect of further research will be based on the study and research, the degree of dispersion of the mixture of seed meal of wheat germ meal and wheat germ.
5. Conclusions
The effect of the defatting process on the chemical composition of wheat germ meal has been studied: the total amount of lipids decreases, the ratio of individual fatty acids changes, and there is a relative increase in the content of the total number of amino acids from 39.0±0.3 % to 42.5±0.5 %. The content of fat-and water-soluble vitamins decreases, which affects the nutritional value of raw materials.
The results of the general digestibility of wheat germ and wheat germ meal have the advisability of using a mixture of meal and wheat germ in a ratio of 80:20 in the recipe composition of dietetic hardtacks, which will make it possible to obtain finished products with a predictably high degree of digestion.
References
[1] Cappelli, A., Cini, E. (2021). Challenges and Opportunities in Wheat Flour, Pasta, Bread, and Bakery Product Production Chains: A Systematic Review of Innovations and Improvement Strategies to Increase Sustainability, Productivity, and Product Quality. Sustainability, 13 (5), 2608. doi: http://doi.org/10.3390/su13052608
[2] Lesnikova, N. A., Kotova, T. V. (2020). Ispolzovanie muki zarodyshei v proizvodstve khleba iz smesi rzhanoi i pshenichnoi muki. XXI vek: itogi proshlogo i problemy nastoiaschego plius, 9 (3), 86-90.
[3] Parenti, O., Guerrini, L., Zanoni, B. (2020). Techniques and technologies for the breadmaking process with unrefined wheat flours. Trends in Food Science & Technology, 99, 152-166. doi: http://doi.org/10.10167j.tifs.2020.02.034
[4] Al-Marazeeq, K. M., Angor, M. M. (2017). Chemical Characteristic and Sensory Evaluation of Biscuit Enriched with Wheat Germ and the Effect of Storage Time on the Sensory Properties for this Product. Food and Nutrition Sciences, 8 (2), 189-195. doi: http://doi.org/10.4236/fns.2017.82012
[14 [15
Demir, M. K., Bilgifli, N., Turker, S., Demir, B. (2021). Enriched Turkish noodles (Eri^te) with stabilized wheat germ: Chemical, nutritional and cooking properties. LWT, 149, 111819. doi: http://doi.org/10.1016/j.lwt.2021.111819 Oliinyk, S., Samokhvalova, O., Lapitska, N., Kucheruk, Z. (2020). Studying the influence of meats from wheat and oat germs, and rose hips, on the formation of quality of ryew heat dough and bread. Eastern-European Journal of Enterprise Technologies, 1 (11 (103)), 59-65. doi: http://doi.org/10.15587/1729-4061.2020.187944
Petrovic, J., Rakic, D., Fistes, A., Pajin, B., Loncarevic, I., Tomovic, V., Zaric, D. (2017). Defatted wheat germ application: Influence on cookies' properties with regard to its particle size and dough moisture content. Food Science and Technology International, 23 (7), 597-607. doi: http://doi.org/10.1177/1082013217713101
Meriles, S. P., Penci, M. C., Steffolani, M. E., Ribotta, P. D. (2020). Effect of heat-treated wheat germ on dough properties and crackers quality. International Journal of Food Science & Technology, 56 (4), 1837-1843. doi: http://doi.org/10.1111/ijfs.14810 Tekgul, Y., Qali^kan Kof, G., Erten, E. S., Akdogan, A. (2020). Determination of the effect of wheat germ on the mineral and fatty acid composition and aroma compounds of tarhana: A traditional fermented cereal food. Journal of Food Processing and Preservation, 45 (2). doi: http://doi.org/10.1111/jfpp.15144
Kostileva, I. E., Izotova, E. D. (2018). Amino acid diversity as a criterion for choosing optimal calibrator for determining crude protein in biological products. Doklady Bashkirskogo universiteta, 3 (4), 490-494.
Vostrikova, N. L., Kuznetsova, O. A., Kulikovskii, A. V. (2018). Methodological aspects of lipid extraction from biological matrices. Theory and Practice of Meat Processing, 3 (2), 4-21. doi: http://doi.org/10.21323/2414-438x-2018-3-2-4-21 Vitman, M. A., Pilipenko, T. V. (2017). Ispolzovanie gazozhidkostnoi khromatografii dlia issledovaniia lipidov sukhogo obo-gaschennogo moloka. Mezhdunarodnaia nauchno-prakticheskaia konferentsiia, posviaschennaia pamiati Vasiliia Matveevi-cha Gorbatova, 1, 59-60.
ZHilinskaia, N. V., Bessonov, V. V., Gromovykh, P. S., Bogachuk, M. N. (2018). Razvitie sovremennoi metodicheskoi bazy kontrolia soderzhaniia vitaminov v pischevoi produktsii i biologicheski aktivnykh dobavkakh k pische. Voprosy pitaniia, 87 (6), 106-116.
Zenkova, M. (2019). Mineral and Amino Acid Composition of Germinated and Canned Wheat Grains. Food Processing: Techniques and Technology, 49 (4), 513-521. doi: http://doi.org/10.21603/2074-9414-2019-4-513-521
Donkov, S. A., Kadetova, M. Iu. (2019). Enzymatic hydrolysis of starch and starch-containing plant raw material in obtaining sugar-containing products for animal husbandry (a review of patents). Vestnik Krasnoiarskogo gosudarstvennogo agrarnogo universiteta, 3 (144), 116-121.
Boukid, F., Folloni, S., Ranieri, R., Vittadini, E. (2018). A compendium of wheat germ: Separation, stabilization and food applications. Trends in Food Science & Technology, 78, 120-133. doi: http://doi.org/10.1016/j.tifs.2018.06.001 Zou, Y., Gao, Y., He, H., Yang, T. (2018). Effect of roasting on physico-chemical properties, antioxidant capacity, and oxidative stability of wheat germ oil. LWT, 90, 246-253. doi: http://doi.org/10.1016/j.lwt.2017.12.038
Novikova, I. V., Sharma, N., Moser, T., Sontag, R., Liu, Y., Collazo, M. J. et. al. (2018). Protein structural biology using cell-free platform from wheat germ. Advanced Structural and Chemical Imaging, 4 (1). doi: http://doi.org/10.1186/s40679-018-0062-9 Yun, L., Li, D., Yang, L., Zhang, M. (2019). Hot water extraction and artificial simulated gastrointestinal digestion of wheat germ polysaccharide. International Journal of Biological Macromolecules, 123, 174-181. doi: http://doi.org/10.1016/j .ijbiomac.2018.11.111
Yun, L., Wu, T., Li, W., Zhang, M. (2021). Wheat germ glycoprotein regionally modulates immunosuppressed mouse intestinal immunity function from early life to adulthood. Food & Function, 12 (1), 97-106. doi: http://doi.org/10.1039/d0fo02754e Dorokhovich, A., Dorokhovich, V., IAremenko, O. (2012). Ispolzovanie zarodyshei pshenitsy kak fiziologicheski funktsio-nalnykh syrevykh ingredientov pri proizvodstve sdobnogo pechenia dlia bolnykh sakharnym diabetom. Nauchni trudove na rusenskiia universite, 51, 40-43.
Rodionova, N. S., Alekseeva, T. V. (2014). The modern theory and technology of production, processing and use of the products of complex processing of wheat germ. Proceedings of the Voronezh State University of Engineering Technologies, 4, 99-109. Rodionova, N. S., Sokolova, O. A. (2016). Formirovanie funktsionalno-tekhnologicheskikh svoistv zhmykha zarodyshei psh-enitsy termicheskoi obrabotkoi. Tekhnologii pischevoi i pererabatyvaiuschei promyshlennosti APK-produkty zdorovogo pi-taniia, 3 (11), 44-50.
Rybakov, Y. S., Lesnikova, N. A., Lavrova, L. Y., Bortsova, E. L., Mazhaeva, T. V. (2014). The use of mechanical activation of wheat germ in manufacture of bakery products. Agrarnii vestnik Urala, 4 (122), 50-53.
