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Section 1. Biology
https://doi.org/10.29013/AJT-22-1.2-3-7
Shermatova G. D., Institute of the Chemistry of Plant Substances named after Acad. S. Yu. Yunusov, Tashkent, Uzbekistan
Rakhimova Sh. Kh., Institute of the Chemistry of Plant Substances named after Acad. S. Yu. Yunusov, Tashkent, Uzbekistan
Komilov B. J.,
Institute of the Chemistry of Plant Substances named after Acad. S. Yu. Yunusov, Tashkent, Uzbekistan
Abdul-Azizovich B. M., Tashkent Botanical Garden named after acad. F. N. Rusanov,
Institute of Botany, AS of Uzbekistan
PROTEIN CONTENT OF SOME RUMEX SPECIES (POLYGONACEAE)
Abstract. The aim of this study is the comparative analysis of the quantitative composition of protein content of five Rumex species: Rumex pamiricus Rech. f., Rumex conglomeratus Murray., Rumex confertus Willd., Rumex acetosa L. and Rumex aquaticus L. growing in similar environmental conditions. Based on the results obtained, it was found that the protein content in the leaves was higher than in the roots and seeds of the existing plants. The protein content of Rumex aquaticus demonstrated the highest values than other species (Table 1). The total proteins content in the roots of Rumex aquaticus 9.25%, in the seeds 9.11%, in the leaves 19.75%. This amount is a high figure for the plant. As a result our research, we have found a new source rich in protein.
Keywords: Polygonaceae L., Rumex pamiricus Rech. f., Rumex conglomeratus Murray., Rumex confertus Willd., Rumex acetosa L., Rumex aquaticus L., sorrel, dock, protein, superfood
1. Introduction Most of the countries of the world have been facing
The nutrients essential for life are proteins, fat malnutrition problems. The deficiency of protein in
and carbohydrates, all contribute to caloric content human food and animal feed is well recognized. The
of the dietary, minerals including trace elements, need of the good quality ofproteins has been increas-
vitamins and water. Numerous studies, including ing due to rapid growth of population. It has been
in man, have demonstrated clearly that life may be reported that in Uzbekistan the protein gap would
sustained by nutrient mixtures in which every com- continue to increase unless well-planned measures
ponent is definable chemically and soluble in water. are adopted to tackle the situation. It is therefore
imperative to increase protein production by utilizing all the available ways and means.
The name of Rumex derived from the Latin word for dart, alluding to the shape ofthe leaves. It is the largest genus of family Polygonaceae [1]. Plants ofthe genus Rumex L. (dock, sorrel) are widely distributed in North America, Central and Eastern Europe, Kazakhstan, the Far East, and partly in the Caucasia, Russia, and East Asia [2; 3; 4; 5]. This genus includes more than 250 species distributed worldwide. 16 species grow in Uzbekistan. Rumex pamiricus Rech. f, Rumex conglomera-tus Murray, Rumex confertus Willd, Rumex acetosa L. and Rumex aquaticus L. are the most common species among them [6; 7; 8]. Since ancient times Rumex L. species have been well known for their use in traditional medicine, due to therapeutic efficacy and various biological activities [9]. The consumption of wild edible plants has been an integral part ofhuman nutrition and traditional medicine since ancient times [10; 11]. Wild plants are known to be a good source of primary nutritional compounds (proteins, fats, sugars, vitamins, and minerals) [12]. Wild plants contain various biologically active components that demonstrated health benefits effects (flavonoids, phenolic acids, anthocyanins, tannins, terpenoids, steroidal saponins, glucosinolates, and so on) [11]. This shows their potential as nutritional supplements, feed additives, and medicinal agents [11; 13]. Among wild plants, Rumex plants have a great
potential. They are already widely used as food, fodder, melliferous, and medicinal plants [5; 14; 15]. In some countries, the leaves of Rumex plants (such as R. vesicarius, R. acetosella, R. abyssinicus, R. crispus, R. in-duratus, R. sanguineus, R. obtusifolius, R. tuberosus, R. thyrsiflorus, and R. acetosa) are used for food, mainly as salads [16; 17]. According to the literature informations, several Rumex species are included in the pharmacopoeias of various countries. For example, Rumex crispus is listed in the American Herbal Pharmacopoeia as a general detoxifier and an agent for skin treatment [18]. The State Pharmacopoeia of the Russian Federation includes the roots of Rumex confertus Willd. as a herbal medicine, which is used in the treatment ofliver diseases, dysentery, pulmonary, and uterine bleeding, as well as a laxative [19; 20]. The aim of this research is to study and compare the amount of protein between of Some Rumex Species.
2. Materials and Methods
2.1. Plant Material
Plant leaves, roots and seeds of the following species were used as the objects of study: Rumex pamiricus Rech. f., Rumex acetosa L., Rumex confertus Willd, Rumex conglomeratus Murray and Rumex aquaticus L. (Figure 1). Leaf samples (during the flowering period on May 2021), seeds and roots (on July, September 2021) of the plants were collected from Botanic Garden, Tashkent, Uzbekistan.
1 2 3 4 5
Figure 1. The leaves of Rumex pamiricus (1); the seeds of Rumex acetosa (2); the seeds of Rumex confertus (3); the roots of Rumex conglomeratus (4); the aerial part of Rumex aquaticus (5)
2.2. Determination of Total Protein Content Nessler reagent. To determine the amount ofprotein, The Kjeldahl method was used to determine the 0.1 g (to the nearest 0.001 g) ofcrushed plant samples amount ofprotein. This method was performed using were placed inside the tubes. To them were added 5
ml of concentrated H2SO4 (r 1,84 g/cm3), 0,5 ml of 30% H202 or 0,2 ml of concentrated perchlorotic acid (HClO4). Place the flasks in a sand bath or on a temperature-controlled plate, set the temperature to 4000C, and heat carefully. After 20 minutes, another 0.5 ml of 30% peroxide or 0.2 ml of concentrated per-chlorotic acid is added to the flask to accelerate mineralization. The solution was heated until completely white (for 1 hour). The flasks were then kept on the plate for another 15-20 minutes and cooled. 10 ml of distilled water was added to the cooled flasks, the required amount of solution was transferred to 50 cm3 flasks, distilled water was added and mixed. After mineralization, 0.3 ml of aliquot solution was added to 25cm3 flasks, and distilled water was added to the 25cm3 mark. 10% NaOH was added to the solution. To eliminate opalescence of the solution, 0.5 ml of 50% Segnet salt solution was first added to the tubes and then 1 ml of Nessler reagent. The solutions were then filled with distilled water to the mark of 50 cm3 and mixed. In this case, the solutions must be clean. If the solutions are turbid, this indicates that the mineralization has not completely passed or that the reagents used are not clean. If the amount of protein in
Table 1. - Protein content of some Rum
the samples is low, the color of the solution will be yellow, iflarge - the color will be orange. After 15 min, the optical density of the solutions was measured at a wavelength of l= 400 nm. The amount of protein in the studied samples was determined according to the following formula:
C _ V*100*5,7*C1
~ H * A *1000 С - protein content,%
H - protein etalon content, g
V - volume, after sample division, ml
A - is the volume of the aliquot taken for colo-
rimetry;
C - is the percentage of nitrogen in the aliquot section determined using a measurement chart, mg 1000 - copying coefficient, mg. 100 - copying coefficient,%. 6.25 - coefficient. 3. Results
In the protein content study of Rumex aquaticus demonstrated the highest values (Table 1). The total proteins content in the roots of Rumex aquaticus 9.25%, in the seeds 9.11%, in the leaves 19.75%. This is a high figure for a plant.
species in the leaves, seeds and roots
№ Plant name Total protein content,%
Root Seed Leaf
1. Rumex pamiricus Rech. f. 5.22 7.56 13.87
2. Rumex confertus Willd 2.87 4.22 15.16
3. Rumexconglomeratus Murray 6.02 5.16 17.14
4. Rumex acetosa L. 5.10 7. 23 16.12
5. Rumex aquaticus L. 9.25 9.11 19.75
Based on the results obtained, the protein content in the leaves was found to be higher than in the roots and seeds of the existing species. It was studied at the Institute of the Chemistry of Plant Substances named after Acad. S. Yu. Yunusov AS of Uzbekistan, Laboratory of Chemistry of high-molecular plant substances. The quantitative protein index of plants was determined at 400 nm on a spectrophotometer device using an improved Nessler reagent of the Kjeldahl method.
4. Discussion
Plants of the genus Rumex have traditionally been used as edible or medicinal plants in various regions of the world. However, today, their biotechnological potential is becoming evident, and these species can act as a resource ofbiologically active substances. The Rumex plants are abundant, undemanding, gain phy-tomass easily, and have a short vegetative cycle (and, as a consequence, can reproduce frequently through-
out the year), thus they have a real advantage among wild plants of the temperate zone. It should also be noted that Rumex species have a high potential for re-growth after injury [20; 21; 22]. Aerial parts of many species (for example, R. confertus, R. acetosella, R. acetosa and R. crispus) are widely used for food. Plants are collected mainly in spring and are used as vegetables [14; 23; 24]. Moreover, the accumulated amount of research allows us to define the plants of this genus as a «superfood». Currently, «superfood» is defined as foods high in nutritional or biologically active phyto-chemicals beneficial to human health [25]. The results of our studies prove that plants of the genus Rumex can occupy a niche in the food industry and act as a functional food product.
5. Conclusion
The aim of this study is the comparative analysis of the quantitative composition of protein content of five Rumex species: R. pamiricus, R. acetosa, R. confertus, R. conglomeratus and Rumex aquaticus growing in similar environmental conditions. As we can see in Table 1, we have found a new source rich in protein. This suggests that Rumex-based foods can be rich sources of protein, and that Rumex species can be a solution to the problem of protein deficiency with their protein-rich content. The results of the study will allow to identify the most promising species for pharmaceutical and food use.
References:
1. Shermatova G. D., Zhang Y.J., Davranov K. Antibacterial and Antifungal Activities of Rumex Confertus Willd. International Journal for Research in Applied Science & Engineering Technology,- 9 (12). 2021.- P. 1855-1856.
2. Rechinger K. H. Vorarbeiten zu einer Monographie der Gattung Rumex. VII. Rumices asiatici. Candol-lea.- 12. 1949.- P. 9-152.
3. Kolodziejek Jeremi. Growth performance and emergence of invasive alien Rumex confertus in different soil types. Scientific Reports.- 9 (1). 2019.- P. 1-13.
4. Kholmatov H. Kh., Habibov Z. N., Olimkhodjaeva N. Z. Medicinal herbs of Uzbekistan. Ibn Sino, 1991.-P. 93-94.
5. Podgurskaya V. V., Luksha E. A., Gushchina E. S., Savchenko I. A., Korneeva I. N., Kalinkina G. I. Biological activity of the genus Rumex (Polygonaceae ) plants. Chem. Plant Raw Mater.- 2. 2021 - P. 59-78.
6. Shermatova G. D., Shamuratov B. A. Flavonoids of Rumex pamiricus. Bulletin of National University of Uzbekistan.- 4/2. 2013.- P. 232-233.
7. Rao K. N. V., Ch S., Banji D. A study on the nutraceuticals from the genus Rumex. Hygeia. J. D. Med.-3(1). 2011.- P. 76-88.
8. Shermatova G. Emodin, an anthraquinone derivative from Rumex pamiricus Rech. f. Universum: chemistry and biology.- 3 (93). 2022.- P. 28-31.
9. Babulka P. The Rumex, from ethobotany to modern phytotherapy (Rumex spp.), [Les Rumex, de l'ethnobotanique a la phytothérapie moderne (Rumex spp.)], Phytothérapie.- 5. 2004.- P. 153-156.
10. Carvalho A. M., Barata A. M. The consumption of wild edible plants. In Wild Plants, Mushrooms and Nuts: Functional Food Properties and Applications; Ferreira, I.C.F.R., Morales, P., Barros, L., Eds. John Wiley & Sons, Inc. Hoboken, NJ, USA, 2017.- P. 159-198.
11. Ceccanti C., Landi M., Benvenuti S., Pardossi A., Guidi L. Mediterranean Wild Edible Plants: Weeds or "New Functional Crops"? Molecules,- 23. 2018.- 2299 p.
12. Puccinelli M., Pezzarossa B., Pintimalli L., Malorgio F. Selenium Biofortification of Three Wild Species, Rumex acetosa L., Plantago coronopus L., and Portulaca oleracea L., Grown as Microgreens. Agronomy.-11. 2021.- P. 1155.
13. Faehnrich B., Franz C., Nemaz P., Kaul H. P. Medicinal plants and their secondary metabolites-State of the art and trends in breeding, analytics and use in feed supplementation with special focus on German chamomile. J. Appl. Bot. Food Qual.- 94. 2021.- P. 61-74.
14. Bello O. M., Fasinu P. S., Bello O. E., Ogbesejana A. B., Adetunji C. O., Dada A. O., Ibitoye O. S., Aloko S., Oguntoye O. S. Wild vegetable Rumex acetosa Linn.: Its ethnobotany, pharmacology and phytochemis-try. A review. S. Afr. J. Bot.- 125. 2019.- P. 149-160.
15. Vasas A., Orban-Gyapai O., Hohmann J. The Genus Rumex: Review of traditional uses, phytochemistry and pharmacology. J. Ethnopharmacol.- 175. 2015.- P. 198-228.
16. Li Y. Jiang J. G. Health functions and structure-activity relationships of natural anthraquinones from plants. Food Funct.- 9. 2018.- P. 6063-6080.
17. Diaz-Munoz G., Miranda I. L., Sartori S. K., de Rezende D. C., Diaz M. A.N. Chapter 11. Anthraquinones: An overview. In Studies in Natural Products Chemistry; Atta-ur-Rahman, B.T.S., Ed. Elsevier: Amsterdam, The Netherlands.- 58. 2018.- P. 313-338.
18. Pinela J., Carvalho A. M., Ferreira I. C. F. R. Wild edible plants: Nutritional and toxicological characteristics, retrieval strategies and importance for today's society. Food Chem. Toxicol.- 110. 2017.- P. 165-188.
19. Upton R., Graff A., Jolliffe G., Länger R. Williamson E. American Herbal Pharmacopoeia: Botanical Pharmacognosy-Microscopic Characterization of Botanical Medicines; CRC Press: Boca Raton, FL, USA. 2016. ISBN1420073281.
20. Emshanova S. V., Potanina O. G., Budanova E. V., Chistyakov V. V.(Eds). Gosudarstvennaya Farmakopeya Rossiyskoy Federatsii XIV izdaniye. In [State Pharmacopoeia of the Russian Federation XIV edition]. Ministry of Health of Russian Federation. Moscow, Russia.- 4. 2018.- 1844 p.
21. Feduraev P., Skrypnik L., Nebreeva S., Dzhobadze G., Vatagina A., Kalinina E., Pungin A., Maslennikov P., Riabova A., Krol O. et al. Variability of Phenolic Compound Accumulation and Antioxidant Activity in Wild Plants of Some Rumex Species (Polygonaceae). Antioxidants.- 11/2. 2022.- 311 p.
22. Chapin F. S., III Schulze E. D., Mooney H. A. The ecology and economics of storage in plants. Annu. Rev. Ecol. Syst.- 21. 1990.- P. 423-447.
23. Zaller J. G. Ecology and non-chemical control of R. crispus and R. obtusifolius (Polygonaceae): A review. Weed Res.- 44. 2004.- P. 414-432.
24. Korpelainen H., Pietiläinen M. Sorrel (Rumex acetosa L.): Not Only a Weed but a Promising Vegetable and Medicinal Plant. Bot. Rev.- 86. 2020.- P. 234-246.
25. Taulavuori K., Julkunen-Tiitto R., Hyöky V., Taulavuori E. Blue Mood for Superfood. Nat. Prod. Commun.- 8. 2013. 1934578X1300800627.
