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PREVALENCE OF TOXOCARA INFECTION IN DOMESTIC DOGS AND CATS IN URBAN ENVIRONMENT
Kurnosova OP1, Odoevskaya IM1 Petkova S2, Dilcheva V2
1 Skryabin All-Russian Scientific Research Institute of Fundamental and Applied Parasitology of Animals and Plants, Moscow
2 Department of Experimental Parasitology,
Institute of experimental morphology, pathology and anthropology with museum, Bulgarian Academy of Sciences, Sofia, Bulgaria
Toxocariasis is the type of helminthic infection found in dogs and cats most often. It is a zoonotic disease that presents a serious threat to the national public health. Urban environment favors transmission of toxocara from animals to people; soil is the key element of such transmission. To learn the degree of toxocara invasion in domestic cats and dogs living in Moscow, we studied their feces for 7 years applying the flotation method. We found that in domestic dogs the intensity of toxocara invasion was 2.43%, but there is a big difference between puppies and adult animals: 5.53% of the former, twice as much as the latter, suffered from the invasion. The intensity of infection in adult cats was 3.97%; kittens, same as puppies, were more prone to host toxocara: 10.44% of those examined did. In general, 5.75% of cats had toxacara, which is twice as much compared to dogs; the figure applies to all ages. Stable infestation of domestic animals with this species of helminths makes them a constant source of toxocara eggs contamination in urban environments, which ups the risk of larvae toxocariasis for people.
Keywords: prevalence of toxocariasis, toxocara, domestic dogs, domestic cats
Funding: this work was supported by the Russian Science Fund, Project #14-1600026.
[><] Correspondence should be addressed: Irina M. Odoevskaya
Bolshaya Cheremushkinskaya 28, Moscow, 117218; [email protected]
Recieved: 16.06.18 Accepted: 10.08.18
DOI: 10.24075/brsmu.2018.044
РАСПРОСТРАНЕНИЕ ТОКСОКАРОЗНОЙ ИНВАЗИИ У ДОМАШНИХ СОБАК И КОШЕК В ГОРОДСКИХ УСЛОВИЯХ
О. П. Курносова1, И. М. Одоевская1 С. Петкова2, В. Дильчева2
1 Всероссийский научно-исследовательский институт экспериментальной и прикладной паразитологии животных и растений имени К. И. Скрябина, Москва
2 Кафедра экспериментальной паразитологии,
Институт экспериментальной морфологии, патологии и антропологии с музеем, Болгарская академия наук, София, Болгария
Токсокароз — один из самых распространенных гельминтозов собак и кошек. Заболевание является зоонозом и представляет серьезную проблему для отечественного здравоохранения. В городской среде создаются благоприятные условия для передачи токсокар от домашних животных к человеку, при этом почва играет ведущую роль в распространении данной инвазии. С целью изучения интенсивности инвазии токсокарами домашних собак и кошек, проживающих на территории г. Москвы, в течение 7 лет проводили исследование фекалий флотационным методом. Показано, что в среднем интенсивность инвазии токсокарами у домашних собак составляет 2,43%, но при этом зараженность щенков в 2 раза выше, чем взрослых особей и составляет 5,53%. Средняя интенсивность инвазии у взрослых кошек составляет 3,97%, а у котят — 10,44%. В целом зараженность кошек токсокарами составляет 5,75%. Исследования показали, что в целом зараженность кошек токсокарозом в 2 раза выше, чем у собак, во всех возрастных группах. Стабильная зараженность домашних животных данным видом гельминтов делает их постоянным источником обсеменения городской среды яйцами токсокар, что создает напряженную эпидемическую обстановку в плане возможности заражения людей ларвальной формой токсокароза.
Ключевые слова: распространенность токсокар, токсокароз, собаки, кошки
Финансирование: работа выполнена при финансовой поддержке гранта РНФ 14-16-00026.
[><1 Для корреспонденции: Ирина Михайловна Одоевская
ул. Большая Черемушкинская, д. 28, Москва, 117218; [email protected]
Статья получена: 16.06.18 Статья принята к печати: 10.08.18
DOI: 10.24075/vrgmu.2018.044
Toxocariasis is a parasitic disease caused by Toxocara nematodes that affects dogs and cats. Mature helminth worms concentrate in the small intestine of carnivores; reaching soil with the excrements of infected animals, toxocara eggs remain alive and capable to invade a host for a long period of time. Toxocariasis in dogs and cats is one of the most common helminth infections in the city of Moscow. Some reports claim that 11.9% to 18.1% of all domestic dogs living in the city suffer from this disease; as for the stray dogs of Moscow, the infection rate is up to 55% [1, 2]. The share of domestic cats hosting toxocara is 11.1%. Prenatal and transmammary
mechanisms of disease transmission makes youngest animals especially susceptible to the invasion of Toxocara nematodes [3]: all (100%) stray puppies and over a half (57.1%) of stray kittens host the worms [4, 5].
Soil is the key media where the larvae survive and through which toxocariasis spreads [6]. The main source of contamination and subsequent invasion is the feces of infected animals; toxocara eggs, especially those capable of infecting animals, are very resistant to the adverse factors of the environment [7]. Analysis of the soil collected in Moscow revealed that the average content of toxocara eggs is 13.5%
(range — from 1.1% to 46.9%); at the playgrounds adjacent to residential housing the figure was 14.8% [8, 9]. One study put the number of toxocara eggs found in 1 kg of the Moscow's soil at 25 to 500 pieces [1]. Contamination was the highest in the areas where people walk their dogs.
Human toxocariasis is primarily a chronic disease. After infection, toxocara larvae migrate through the human organism carried by lymph and blood flow, which results in damage to various tissues and organs and sensitization by the parasite's metabolism byproducts. Clinical manifestations of human toxocariasis are varied, but generally they translate into damage to internal organs, liver and lungs for the most part, but also eyes and the central nervous system. Cutaneous toxocariasis causes changes peculiar to larva migrans. There is a number of factors that define the severity of the disease, including the number of infectious eggs swallowed, frequency of reinvasion, response of the organism to the damage done and antigenic effect produced by migrating larvae, their concentration site. Combined, such factors shape the development of pathological changes and the disease prognosis in general [10].
Most often, Toxocara nematodes invade adults who often contact animals and/or contaminated soil (occupational hazard) and children whose personal hygiene skills are yet insufficient. Infection occurs more often in rural areas, where contact with soil is more common and domestic animals are not subjected to preventive dehelmintization [11]. However, the well-developed social infrastructure in big cities does not eliminate the threat of contracting toxocariasis [12]. The number of domestic dogs grows, and stray dogs and cats migrate freely, which translates into the growing number of toxocara infested sites in the cities and ensures continued activity of those that already exist. Quite often, numerous owners of domestic dogs walk their pets in small parks and squares and do not pick the excrements their animals leave there. As a rule, dogs and cats enter playgrounds freely, and sandboxes have no lids or tarpaulins. Stray cats cover their excrements with sand or loose earth, which turns
Table 1. Indices of Infection by Toxocara of domestic dogs
playground sandboxes into toxocariasis infection reservoirs dangerous to children.
Throughout the country, researchers analyze the spread of human toxocariasis, as well as the degree of the population's seropositivity to the antigens of these nematodes. The figures received are various. Overall, the occurrence of human toxocariasis in Russia doubled from 2003 to 2007 [12]. For example, in Tula the level of seropositivity is 19.3%, in Ekaterinburg — 2%, in Vladivostok — 8.6%, up to 20.8% in the Altai Republic, in Moscow — up to 17%. In the south of Russia the infection rate is 14.6 to 36.4%, which is 2-3 times higher than in other parts of the country [5, 12-15].
The role T. canis and T. cati nematodes play in the current situation is yet to be established. Single cases of feline toxocara infection in human beings were registered (damage to eyes and internal organs reported) [16]. Current examination methods, serological identification of toxocara do not allow establishing the type of nematodes the patient is infected with.
The purpose of our research was to investigate the prevalence of toxocara invasion of gastrointestinal tract of domestic dogs and cats in Moscow.
METHODS
The study lasted 7 years, from 2011 to 2017. Pasteur veterinary lab and the K. I. Skryabin All-Russian Scientific Research Institute of Fundamental and Applied Parasitology of Animals and Plants were the facilities where we analyzed 1632 samples of feces of domestic dogs and 1146 sample of feces of domestic cats. The samples were delivered to the laboratory in the special plastic or glass containers.
The feces were examined using ammonium nitrate (density of 1.24), flotation method. Biological microscope Biolam (LOMO; Russia) was used to study the samples, magnification x10, x40. After examination, samples were decontaminated through autoclaving.
Year Puppies, total Infected, of them EI (%) Adult dogs Infected, of them EI (%) Total Infected, of them EI (%)
2011 13 1 7.69 35 1 2.85 48 2 4.16
2012 83 2 2.4 225 3 1.33 308 5 1.62
2013 74 8 10.8 219 3 1.36 293 11 3.75
2014 64 3 4.68 195 3 1.53 259 6 2.31
2015 97 4 4.12 201 2 14.3 298 6 2.01
2016 75 3 4 185 2 1.08 260 5 1.92
2017 46 4 8.6 120 1 0.83 166 5 3.01
Total 452 25 5.53 1180 15 1.27 1,632 40 2.45
Note: EI — the extensity of infestation.
Table 2. Indices of infection by Toxocara in domestic cats
Year Kittens, total Infected, of them EI (%) Adult cats Infected, of them EI (%) Total Infected, of them EI (%)
2011 19 1 5.26 57 1 1.75 76 2 2.63
2012 51 6 11.76 151 1 0.66 202 7 3.46
2013 56 7 12.5 147 6 4.08 203 13 6.4
2014 57 6 10.5 127 4 3.14 184 10 5.43
2015 56 5 8.92 140 4 2.85 196 9 4.59
2016 51 5 9.1 121 7 5.78 172 12 6.97
2017 26 3 11.53 87 10 11.49 113 13 11.5
Total 316 33 10.44 830 33 3.97 1,146 66 5.75
Note: EI — the extensity of Infestation.
RESULTS
The average rate of toxocara infestation in domestic dogs is 2.45% (Table 1). Throughout the term of the research, we have witnessed various degree of infection: in adult animals it ranged from 0.83 to 2.85%, in puppies from 2.4 to 10.8%. On the whole, the prevalence on the parasite infection in puppies is two times higher than in adult dogs, reaching an average of 5.53% (Fig. 1).
Table 2 shows the data on toxocara infestation in domestic cats: the average rate for adult animals is 3.97%, minimum registered in 2012 (0.66%) and maximum in 2017 (11.49%). Same as puppies, kittens suffer infestations more often than adult cats (10.44%). Overall, 5.75% of cats host toxocara. Figure 2 and Figure 3 show the results of studies reporting that cats are infested with such nematodes twice as often as dogs (both adult and young animals).
DISCUSSION
Analysis of literature describing the like research efforts has shown that toxocara infestation in dogs and cats is the one diagnosed more often than other intestinal helminthiases, but the rates of infestation vary [4, 18-20]. The studies report that up to 7.3% of adult domestic dogs suffer the infection, while the share of toxocara nematodes hosts among puppies is 30.2%; the figures for cats are 11.1% (adult animals) and up to 33.3% (kittens) [4]. Researchers that studied stray dogs and cats have registered the maximum infestation rate in puppies (100%) and kittens (57.1%) [4]. Such differences in research depend on the category of animals selected. For example, the data we have outlined above describes domestic cats and dogs and shows that the toxocara infection rate among them is quite low. This may be due to the specifics of parasitological research in a commercial laboratory, which is the destination veterinarians send their patients to for analysis when there is a suspected case of toxocariasis or owners of domestic dogs and cats wish to subject their pets to examination.
Nonintensive invasions without clinical symptoms peculiar to toxocariasis often cancel the need to suspect that the animal suffers this type of helminthiasis. There is no doubt that a general screening of all domestic dogs and cats would produce higher infestation rates.
beings. Epidemiologists need to regularly examine soil and samples from sandboxes in search for toxocara eggs, as well as recommend property managing companies to fence playgrounds in order to prevent neglected stray animals from entering them.
Fig. 1. T. canis eggs
Fig. 2. T. cati egg
CONCLUSIONS
The study conducted has shown that there is a functioning site of toxocara infestation within the boundaries of the megalopolis of Moscow. This nature of this disease is zoonotic; it poses serious social and medical dangers since the infection can be contracted by people via the components of their environment contaminated with invasive toxocara eggs.
Thus, timely and regular laboratory examinations, treatment and preventive dehelmintization of pets would allow reducing the risk of contamination of urbanized territory with toxocara eggs. Stray animals have not been subjected to such activities for a long period of time, which made them a source of infestation targeting both other dogs and cats and human
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Fig. 3. T. cati invasive egg
References
1. Peshkov RA. Jepizootologicheskaja situacija po toksokarozu u plotojadnyh i gel'mintologicheskaja ocenka vneshnej sredy v megapolise Moskva [dissertacija]. M.: 2010.
2. Peshkov RA. Gel'mintofauna sobak i koshek v uslovijah g. Moskvy. V sbornike: Teorija i praktika bor'by s parazitarnymi boleznjami: materialy dokladov nauchnoj konferencii VIGIS. 2007; (8): 277-8.
3. Gorohov VV, Peshkova RA, Gorohova EV. Toksokaroz kak jekologicheskaja problema. Veterinarnaja patologija. 2009; (1): 10-12.
4. Panova OA. Toksokaroz plotojadnyh: metody diagnostiki i biojekologicheskie aspekty razvitija vozbuditelej v uslovijah megapolisa [dissertacija]. M.: 2011.
5. Pautova EA, Dovgalev AS, Astanina SJu. Toksokaroz u detej i podrostkov s allergicheskimi i bronholegochnymi zabolevanijami, grupp riska po VICh-infekcii, gepatitam V i S (rezul'taty serologicheskogo skrininga. Med. parazitol. 2013; (2): 13-17.
6. Zaichenko IV. Gel'mintozy plotojadnyh gorodskoj populjacii (rasprostranenie, diagnostika, lechenie) [dissertacija]. Stavropol': 2012.
7. Masalkova JuJu. Osobennosti vozdejstvija ul'trazvuka na jajca Toxocara canis. Rossijskij parazitologicheskij zhurnal. 2014; (1): 52-56.
8. Guzeeva MV. Sovremennaja situacija po toksokarozu v Moskve. Med. parazitol. 2009; (1): 49-51.
9. Uspenskij AV, Peshkov RA, Gorohov VV, Gorohova EV. Toksokaroz v sovremennyh uslovijah. Med. parazitol. 2011; (2): 3-6.
10. Sergiev VP, Lobzin JuV, Kozlova SS, redaktory. Parazitarnye bolezni cheloveka. SPb.: Foliant; 2006. 592 s.
11. Slobodenjuk AV, Kosova AA, Rukoleeva SI. Osobennosti
rasprostranenija toksokaroza na territorii sel'skogo i gorodskogo tipa. Med. parazitol. 2005; (3): 36-8.
12. Guzeeva MV. Sovremennaja situacija po toksokarozu v Moskve. Med. parazitol. 2009; (1): 49-51.
13. Derzhavina TJu. Monitoring za geogel'mintozami u ljudej v Tul'skoj oblasti. Med. parazitol. 2010; (30): 42-4.
14. Ermolenko AV, Rumjanceva EE, Bartkova AD, Voronok VM, Poljakova LF. Nematodozy u ljudej v Primorskom krae. Med. parazitol. 2013; (1): 31-5.
15. Espinoza Y. A., Huapaya P. E., Roldan W. HSeroprevalence of human toxocariasis in Andean communities from the Northeast of Lima. Rev Inst Med Trop Sau Paulo. 2010; 52 (1): 31-36. DOI: 10.1590/S0036-46652010000100006.
16. Fogt-Wyrwas R, Jarosz W, Mizgajska-Wiktor H. Utilizing a polymerase chain reaction method for the detection of Toxocara canis and T. cati eggs in soil. J Helminthol. 2007; 81(1): 75-8. DOI:10.1017/S0022149X07241872.
17. Kotelnikov GA. Diagnostika gel'mintozov zhivotnyh. M.: Kolos; 1974. 240 s.
18. Kurnosova OP. Rasprostranenie prostejshih Lamblia (Giardia) sp. sredi sobak i koshek goroda Moskvy. Med. parazitol. 2014; (3): 23-5.
19. Skripova LV. Parazitologicheskaja situacija na ob"ektah dlja pit'evogo i hozjajstvenno-bytovogo vodosnabzhenija. Zdravoohranenie. 2010; (6): 22-3.
20. Shishkanova LV. Toksokaroz na juge Rossii (jepizootologicheskaja, sanitarno-parazitologicheskaja i serojepidemiologicheskaja harakteristika) [disertacija]. M.: 2011.
Литература
1. Пешков Р. А. Эпизоотологическая ситуация по токсокарозу у плотоядных и гельминтологическая оценка внешней среды в мегаполисе Москва [диссертация]. М.: 2010.
2. Пешков Р. А. Гельминтофауна собак и кошек в условиях г. Москвы. В сборнике: Теория и практика борьбы с паразитарными болезнями: материалы докладов научной конференции ВИГИС. 2007; (8): 277-8.
3. Горохов В. В., Пешкова Р. А., Горохова Е. В. Токсокароз как экологическая проблема. Ветеринарная патология. 2009; (1): 10-12.
4. Панова О. А. Токсокароз плотоядных: методы диагностики и биоэкологические аспекты развития возбудителей в условиях мегаполиса [диссертация]. М.: 2011.
5. Паутова Е. А., Довгалев А. С., Астанина С. Ю. Токсокароз у детей и подростков с аллергическими и бронхолегочными заболеваниями, групп риска по ВИЧ-инфекции, гепатитам В и С (результаты серологического скрининга. Мед. паразитол. 2013; (2): 13-17.
6. Заиченко И. В. Гельминтозы плотоядных городской популяции (распространение, диагностика, лечение) [диссертация]. Ставрополь: 2012.
7. Масалкова Ю. Ю. Особенности воздействия ультразвука на яйца Toxocara canis. Российский паразитологический журнал. 2014; (1): 52-56.
8. Гузеева М. В. Современная ситуация по токсокарозу в Москве. Мед. паразитол. 2009; (1): 49-51.
9. Успенский А. В., Пешков Р. А., Горохов В. В., Горохова Е. В. Токсокароз в современных условиях. Мед. паразитол. 2011; (2): 3-6.
10. Сергиев В. П., Лобзин Ю. В., Козлова С. С., редакторы.
Паразитарные болезни человека. СПб.: Фолиант; 2006. 592 с.
11. Слободенюк А. В., Косова А. А., Руколеева С. И. Особенности распространения токсокароза на территории сельского и городского типа. Мед. паразитол. 2005; (3): 36-8.
12. Гузеева М. В. Современная ситуация по токсокарозу в Москве. Мед. паразитол. 2009; (1): 49-51.
13. Державина Т. Ю. Мониторинг за геогельминтозами у людей в Тульской области. Мед. паразитол. 2010; (30): 42-4.
14. Ермоленко А. В., Румянцева Е. Е., Барткова А. Д., Воронок В. М., Полякова Л. Ф. Нематодозы у людей в Приморском крае. Мед. паразитол. 2013; (1): 31-5.
15. Espinoza Y. A., Huapaya P. E., Roldan W. HSeroprevalence of human toxocariasis in Andean communities from the Northeast of Lima. Rev Inst Med Trop Sau Paulo. 2010; 52 (1): 31-36. DOI: 10.1590/S0036-46652010000100006.
16. Fogt-Wyrwas R, Jarosz W, Mizgajska-Wiktor H. Utilizing a polymerase chain reaction method for the detection of Toxocara canis and T. cati eggs in soil. J Helminthol. 2007; 81(1): 75-8. D0I:10.1017/S0022149X07241872.
17. Котельников Г. А. Диагностика гельминтозов животных. М.: Колос; 1974. 240 с.
18. Курносова О. П. Распространение простейших Lambfe (Giardia) sp. среди собак и кошек города Москвы. Мед. паразитол. 2014; (3): 23-5.
19. Скрипова Л. В. Паразитологическая ситуация на объектах для питьевого и хозяйственно-бытового водоснабжения. Здравоохранение. 2010; (6): 22-3.
20. Шишканова Л. В. Токсокароз на юге России (эпизоотологическая, санитарно-паразитологическая и сероэпидемиологическая характеристика) [дисертация]. М.: 2011.
