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POTENTIAL OF NON-TRADITIONAL CELL CULTURES FOR PRODUCTION OF BIOTHERAPEUTIC PROTEINS
Dobronos MA1-2, Osipova ZM1-3 Myshkina NM1
1 Shemyakin-Ovchinnikov Institute of Bioorganic Chemistry RAS, Moscow, Russia
2 Moscow Institute of Physics and Technology (MIPT), Dolgoprudny, Russia
3 Pirogov Russian National Research Medical University, Moscow, Russia
Production of biotherapeutic drugs in mammalian cells, recombinant proteins in particular, may be handicapped by the limitations imposed on the cultures by metabolic burden. An alternative solution is to produce proteins in cells of other animals (e.g., Sf9, S2 and High Five insect cell lines, Caenorhabditis elegans and Schistosoma mansoni cell line) or orthogonal cell systems, including plant-based. In our opinion, non-traditional cell cultures may become promising tool for production of affordable and effective biotherapeutic drugs.
Keywords: biotherapeutic drugs, plant cell cultures, metabolic burden, High Five cell line, Sf9 cell line
Funding: the work was supported by the Russian Science Foundation grant No. 21-74-10152, https://rscf.ru/project/21-74-10152/
Acknowledgements: the authors thank A.D. Barykin from the Department of Bioorganic Chemistry of the Institute of Bioorganic Chemistry of the Russian Academy of Sciences for the fruitful discussion of the idea of this publication.
Author contribution: Dobronos MA — literature search and analysis, manuscript authoring; Osipova ZM — project management, manuscript editing; Markina NM — manuscript idea, literature search and analysis, manuscript authoring.
1Ж1 Correspondence should be addressed: Zinaida Mikhailovna Osipova Miklukho-Maklaya, 16/10, Moscow, 117997; zkaskova@ibch.ru
Received: 20.04.2024 Accepted: 12.05.2024 Published online: 13.06.2024
DOI: 10.24075/brsmu.2024.022
ПОТЕНЦИАЛ НЕКЛАССИЧЕСКИХ КЛЕТОЧНЫХ КУЛЬТУР ДЛЯ ПРОИЗВОДСТВА БИОТЕРАПЕВТИЧЕСКИХ БЕЛКОВ
М. А. Добронос1,2, З. М. Осипова1,3 Н. М. Мышкина1
1 Институт биоорганической химии имени М. М. Шемякина и Ю. А. Овчинникова, Москва, Россия
2 Московский физико-технический институт, Долгопрудный, Россия
3 Российский национальный исследовательский медицинский университет имени Н. И. Пирогова, Москва, Россия
Производство биотерапевтических препаратов, в частности, рекомбинантных белков в клетках млекопитающих может быть затруднено из-за ограничений используемых культур в связи с метаболической нагрузкой. Альтернативным подходом для решения таких задач является наработка белков в клетках других животных (например, культуры клеток насекомых Sf9, S2 и High Five, культуры клеток червей видов Caenorhabditis elegans и Schistosoma mansoni) или ортогональных клеточных системах, в том числе растительных. С нашей точки зрения, применение неклассических клеточных культур может стать перспективным направлением для получения более доступных и эффективных биотерапевтических препаратов.
Ключевые слова: биотерапевтические препараты, растительные клеточные культуры, метаболическая нагрузка, клеточная линия High Five, клеточная линия Sf9
Финансирование: работа выполнена при поддержке гранта Российского научного фонда 21-74-10152, https://rscf.ru/project/21-74-10152/.
Благодарности: авторы благодарят А. Д. Барыкина из отдела биоорганической химии ИБХ РАН за плодотворное обсуждение идеи публикации.
Вклад авторов: М. А. Добронос — поиск и анализ литературы, написание рукописи; З. М. Осипова — руководство проектом, редактирование рукописи; Н. М. Маркина — идея рукописи, поиск и анализ литературы, написание рукописи.
[X] Для корреспонденции: Зинаида Михайловна Осипова
ул. Миклухо-Маклая, д. 16/10, г. Москва, 117997; zkaskova@ibch.ru
Статья получена: 20.04.2024 Статья принята к печати: 12.05.2024 Опубликована онлайн: 13.06.2024 DOI: 10.24075/vrgmu.2024.022
Today, one of the key tasks before the pharmaceutical industry is to increase the efficacy of production of biotherapeutic drugs. Depending on the desired composition of the drug, the stages that may present hindering obstacles are the search for a natural source thereof or the development of its artificial analogue, boosting production by the source, or optimization of purification (removal of impurities and ineffective forms) [1, 2]. We would like to present the potential of non-traditional cell cultures as a key part of the solutions to such problems.
Cell cultures are most common in the production of biotherapeutic protein preparations, with monoclonal antibodies being the most significant thereof. The flagship culture is that of Chinese hamster ovary cells (CHO): it is easy to cultivate and grows rapidly, guarantees correct translation, folding and
posttranslational modification of the recombinant protein, and releases large amounts of the product into the culture medium, yielding the largest amounts of the target drug among all mammalian cell cultures [3]. The drawbacks of all mammalian cell cultures, including CHO, are high cost and need for special working conditions and equipment, as well as susceptibility to metabolic burden. When the output of the recombinant protein reaches a certain level, this burden prevents standard production boosting technologies from working, including those that involve increasing the number of copies of the recombinant gene, using stronger regulatory sequences, etc. [4]. This happens because recombinant processes begin to compete for resources with the host cell's viability maintenance processes; there are about 8-10 of these in total, including the processes of
Table. Comparison of the most common types of expressing cell cultures [11, 24, 25]
Expression platform Advantages Flaws Post-translational modifications Cost, complexity of purification Scaling potential Safety
Bacteria (E coll) Low cost Simple genetic engineering process Rapid growth of culture and high yield of the target product Proven expression optimization strategies Incorrect folding of some proteins and formation of inclusion bodies. Presence of endotoxins Non-native No glycosylation Difficulties with formation of disulfide bonds Low +++ Moderate
Yeasts (P. pastoris, S. cerevisiae) Low cost Simple genetic engineering process Rapid growth of culture and high yield of the target product Proven optimization strategies Correct folding of large (> 30 kDa) proteins Cell wall can handicap purification Non-native There are strains with limited glycosylation capabilities Low +++ Moderate
Plant-based systems (BY-2, NT-1) Rapid growth of culture and high yield of the target product Possibility of expression of multiprotein complexes Genetic instability of lines during long-term cultivation Increased risk of culture contamination Non-native (genetic vectors optimization required) Moderate / None for edible plants +++ Very high
Insect cells (Sf21, Sf9, Hi5) Expression of eukaryotic multiprotein complexes with correct folding Higher product yields Expression with strong promoters can disrupt folding Non-targeted glycosylation Simplified N-glycosylation Moderate +++ Low
Mammalian cells (CHO, HEK293) Native lipid environment and folding conditions Possibility of inducible expression by transient transfection Possibility of using FACS (Fluorescence Activated Cell Sorting) on stable lines Low expression level Overexpression of some proteins is impossible due to toxicity Long-term optimization of expression conditions Native Moderate ++ Low
Cell-free expression systems Fast expression method Possibility of producing toxic proteins Detailed control of environmental parameters during expression High cost Lack of in vivo factors enabling folding Need for additional components (EPR microsomes, etc.) Low + Very high
transcription, translation, post-translational modifications, and protein export [5]. Various metabolism balancing techniques are used to counter the negative effects of metabolic burden, but this is a very labor-intensive process, since it is necessary to identify all the limiting stages [6] and choose the method to overcome them without compromising the overall viability of the producing cell [7, 8]. However, even successful metabolism balancing may not yield a significant boost in recombinant protein production, since in the case of some biotherapeutic proteins, the process is so laborious for a mammalian host cell that all attempts to optimize it are limited by the physiological capabilities of that cell. For example, recombinant production of the blood coagulation factor VIII (F8) in the CHO culture has the approximate "energy cost" of about 10,000 ATP molecules per a functional F8 molecule [5].
As an alternative, protein preparations can be produced in hosts whose physiological resources are initially higher than those of mammalian cells; such hosts are cells from other animal species or orthogonal cellular systems, like plant cell cultures (Table). One of the main advantages of plant-based biotherapeutic compounds is their safety: they cannot be infected with human pathogens, produce no endotoxins, and have reduced immunogenicity, which improves drug tolerance and minimizes side effects. For example, taliglucerase alpha (P-D-glucosyl-N-acylsphingosinglucohydrolase) produced in transgenic carrot cells for treatment of Gaucher disease type 1 has shown to not trigger any evident side effects associated with N-glycan residues during clinical trials. Moreover, no
antibodies to this drug have been detected [9]. In addition, biological preparations produced in plant cell cultures can be administered orally without purification or with minimal purification. Plant cell walls can protect biological products from enzymatic degradation in the gastrointestinal tract, as well as facilitate the delivery of these drugs to the intestine lymphoid tissue in the active form. Clinical trials have shown that production of oral biopharmaceuticals from edible plant tissues is feasible [10].
Plant cell cultures allow achieving a high level of expression of multiprotein complexes that require complex folding and assembly processes, which is also an important aspect in the context of their use for the purpose. Strategies involving construction of a single vector with a set of recombinant genes and joint biosynthesis of recombinant proteins together with chaperones of the same origin can help increase the output of such complexes [11]. In addition, introduction of an exogenous signal sequence directing the protein along a specific secretory pathway can increase the yield of small proteins weighing less than 30 kDa. We believe that optimization of the fermentation process, including continuous or semi-continuous fermentation, is a universal method of increasing protein output from both plant and insect cell cultures.
Insect cell lines Spodoptera frugiperda Sf21, Sf9 and Trichoplusia ni BTI- 5B1-4 (High Five), adherent nonpermissive cell cultures obtained from ovarian tissues of the respective insects, are also widely in production of biotherapeutic proteins in baculovirus expression systems [12]. Insect cell cultures
offer similar mechanisms of post-translational modification of proteins, which makes them a cost-effective and scalable tool for the production of vaccine antigens and virus-like particles [13]. Besides, engineered baculoviruses with mammalian promoters (BacMam) possess a significant potential as vectors for gene delivery to mammalian cells [14]. The glycosylation pattern in these expression systems differs slightly from that of humans, but can be humanised through parallel expression of mammalian glycotransferases and the removal of insect-specific alpha-1,3-fucosylated glycans, which can cause allergies in people. Antigen proteins that are core of candidate vaccines against COVID-19 [15-17] and malaria [18] are produced in insect cell cultures.
CRISPR technology can help significantly accelerate the production of stably expressing glycoengineered insect lines. It has been shown that CRISPR can be used to knock out genes in the Drosophila and Bombyx cell lines, as well as to knock out the N-acetylglucosaminidase gene in the S2 cell line, which triggers exponential growth of the number of GlcNAc terminal residues in recombinant human erythropoietin [19]. Another promising direction is modification of the Sf9 and High Five cell lines, e.g., multiple duplication of the mammalian glycotransferase genes, can secure an even higher level of protein expression with correct glycosylation and folding.
Worm cell lines are a viable alternative for a number of biomedical applications. For example, embryonic C. elegans cell cultures are used to study the processes of cell differentiation, morphogenesis, and gene expression, opening up a wide range
of previously experimentally inaccessible opportunities [20]. Somatic cells from various tissues of C. elegans (neurons, muscle cells, hypodermic and intestinal cells, etc.) can be cultured for investigation of tissue-specific interactions and signaling pathways [21]. Cell culture of Schistosoma mansoni, a parasitic flatworm, which can be continuously cultivated for 6 months [22], may also become an interesting tool for studying the parasite-host interactions and anthelmintic drugs testing. Many species of marine worms are sources of biologically active compounds, including peptides with antimicrobial, anti-inflammatory, immunomodulatory, antioxidant, and antihypoxic effects [23]. The development and optimization of the technology to isolate and cultivate worm cells (long-term cultivation) could boost screening and subsequent effective development of such biologically active substances.
CONCLUSION
Thus, the use of nontraditional cell cultures is a promising way to increase the efficiency of the production of biotherapeutic drugs. Several features of expression mechanisms in alternative cultures can minimize side effects and improve tolerability of the resulting protein preparations. Moreover, alternative producing organisms also help to circumvent the limitations associated with the increased metabolic burden in mammalian cell cultures. This enables further development and production of more effective and affordable biotherapeutic drugs, contributing to the overall progress in the fields of pharmaceuticals and medicine.
References
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3. Meleady P, Doolan P, Henry M, Barron N, Keenan J, O'Sullivan F, et al. Sustained productivity in recombinant Chinese Hamster Ovary (CHO) cell lines: proteome analysis of the molecular basis for a process-related phenotype. BMC Biotechnol. 2011; 11: 78. Available from: https://doi.org/10.1186/1472-6750-11-78.
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5. Gutierrez JM, Feizi A, Li S, Kallehauge TB, Hefzi H, Grav LM, et al. Genome-scale reconstructions of the mammalian secretory pathway predict metabolic costs and limitations of protein secretion. Nat Commun. 2020; 11: 68. Available from: https://doi.org/10.1038/s41467-019-13867-y.
6. Kaste JAM, Shachar-Hill Y. Model validation and selection in metabolic flux analysis and flux balance analysis. Biotechnol Prog. 2024; 40: e3413. Available from: https://doi.org/10.1002/btpr.3413.
7. Eisenhut P, Marx N, Borsi G, Papez M, Ruggeri C, Baumann M, et al. Manipulating gene expression levels in mammalian cell factories: An outline of synthetic molecular toolboxes to achieve multiplexed control. New Biotechnol. 2024; 79: 1-19. Available from: https://doi.org/10.1016Zj.nbt.2023.11.003.
8. Kol S, Ley D, Wulff T, Decker M, Arnsdorf J, Schoffelen S, et al. Multiplex secretome engineering enhances recombinant protein production and purity. Nat Commun. 2020; 11: 1908. Available from: https://doi.org/10.1038/s41467-020-15866-w.
9. Aviezer D, Brill-Almon E, Shaaltiel Y, Hashmueli S, Bartfeld D, Mizrachi S, et al. A Plant-Derived Recombinant Human Glucocerebrosidase Enzyme — A Preclinical and Phase I Investigation. PLOS ONE. 2009; 4: e4792. Available from: https://doi.org/10.1371/journal.pone.0004792.
10. Fausther-Bovendo H, Kobinger G. Plant-made vaccines and therapeutics. Science 2021; 373: 740-1. Available from: https://doi.org/10.1126/science.abf5375.
11. Hellwig S, Drossard J, Twyman RM, Fischer R. Plant cell cultures for the production of recombinant proteins. Nat Biotechnol. 2004; 22: 1415-22. Available from: https://doi.org/10.1038/nbt1027.
12. Woo S-D, Roh JY, Choi JY, Jin BR. Propagation of Bombyx mori Nucleopolyhedrovirus in nonpermissive insect cell lines. J Microbiol Seoul Korea. 2007; 45: 133-8.
13. Hong M, Li T, Xue W, Zhang S, Cui L, Wang H, et al. Genetic engineering of baculovirus-insect cell system to improve protein production. Front Bioeng Biotechnol. 2022; 10. Available from: https://doi.org/10.3389/fbioe.2022.994743.
14. Hu Y-C, Yao K, Wu T-Y. Baculovirus as an expression and/or delivery vehicle for vaccine antigens. Expert Rev Vaccines. 2008; 7: 36371. Available from: https://doi.org/10.1586/14760584.7.3.363.
15. Fernandes B, Castro R, Bhoelan F, Bemelman D, Gomes RA, Costa J, et al. Insect Cells for High-Yield Production of SARS-CoV-2 Spike Protein: Building a Virosome-Based COVID-19 Vaccine Candidate. Pharmaceutics. 2022; 14: 854. Available from: https://doi.org/10.3390/pharmaceutics14040854.
16. Struble LR, Smith AL, Lutz WE, Grubbs G, Sagar S, Bayles KW, et al. Insect cell expression and purification of recombinant SARS-COV-2 spike proteins that demonstrate ACE2 binding. Protein Sci. 2022; 31: e4300. Available from: https://doi.org/10.1002/pro.4300.
17. Li T, Zheng Q, Yu H, Wu D, Xue W, Xiong H, et al. SARS-CoV-2 spike produced in insect cells elicits high neutralization titres in non-human primates. Emerg Microbes Infect. 2020; 9: 2076-90. Available from: https://doi.org/10.1080/22221751.2020.1821583.
18. Fernandes B, Sousa M, Castro R, Schäfer A, Hauser J, Schulze K, et
al. Scalable Process for High-Yield Production of PfCyRPA Using Insect Cells for Inclusion in a Malaria Virosome-Based Vaccine Candidate. Front Bioeng Biotechnol. 2022; 10. Available from: https://doi.org/10.3389/fbioe.2022.879078.
19. Mabashi-Asazuma H, Kuo C-W, Khoo K-H, Jarvis DL. Modifying an Insect Cell N-Glycan Processing Pathway Using CRISPR-Cas Technology. ACS Chem Biol. 2015; 10: 2199-208. Available from: https://doi.org/10.1021/acschembio.5b00340.
20. Strange K, Christensen M, Morrison R. Primary culture of Caenorhabditis elegans developing embryo cells for electrophysiological, cell biological and molecular studies. Nat Protoc. 2007; 2: 1003-12. Available from: https://doi.org/10.1038/nprot.2007.143.
21. Espejo L, Hull B, Chang LM, DeNicola D, Freitas S, Silbar V, et al. Long-Term Culture of Individual Caenorhabditis elegans on Solid Media for Longitudinal Fluorescence Monitoring and Aversive Interventions. JoVE J Vis Exp. 2022: e64682. Available from:
https://doi.org/10.3791/64682.
22. Hobbs DJ, Fryer SE, Duimstra JR, Hedstrom OR, Brodie AE, Collodi PA, et al. Culture of Cells from Juvenile Worms of Schistosoma mansoni. J Parasitol. 1993; 79: 913-21. Available from: https://doi.org/10.2307/3283730.
23. Lazcano-Perez F, Roman-Gonzalez SA, Sanchez-Puig N, Espinosa RA-. Bioactive Peptides from Marine Organisms: A Short Overview. Protein Pept Lett. 2002; 19: 700-7. Available from: https://doi.org/10.2174/092986612800793208.
24. Jayakrishnan A, Wan Rosli WR, Tahir ARM, Razak FSA, Kee PE, Ng HS, et al. Evolving Paradigms of Recombinant Protein Production in Pharmaceutical Industry: A Rigorous Review Sci. 2024; 6: 9. Available from: https://doi.org/10.3390/sci6010009.
25. Cid R, Bolívar J. Platforms for Production of Protein-Based Vaccines: From Classical to Next-Generation Strategies. Biomolecules. 2021; 11: 1072. Available from: https://doi.org/10.3390/biom11081072.
Литература
1. Daboussi F, Lindley ND. Challenges to Ensure a Better Translation of Metabolic Engineering for Industrial Applications. In: Selvarajoo K, editor. Comput Biol Mach Learn Metab Eng Synth 14. Biol., New York, NY: Springer US; 2023: 1-20. Available from: https://doi.org/10.1007/978-1-0716-2617-7_1.
2. Rudge SR, Ladisch MR. Industrial Challenges of Recombinant 15. Proteins. In: Silva AC, Moreira JN, Lobo JMS, Almeida H, editors.
Curr Appl Pharm Biotechnol, Cham: Springer International Publishing; 2020: p. 1-22. Available from: https://doi.org/10.1007/10_2019_120.
3. Meleady P, Doolan P, Henry M, Barron N, Keenan J, O'Sullivan
F, et al. Sustained productivity in recombinant Chinese Hamster 16. Ovary (CHO) cell lines: proteome analysis of the molecular basis for a process-related phenotype. BMC Biotechnol. 2011; 11: 78. Available from: https://doi.org/10.1186/1472-6750-11-78.
4. Frei T, Cella F, Tedeschi F, Gutiérrez J, Stan G-B, Khammash M, et 17. al. Characterization and mitigation of gene expression burden in mammalian cells. Nat Commun. 2020; 11: 4641. Available from: https://doi.org/10.1038/s41467-020-18392-x.
5. Gutierrez JM, Feizi A, Li S, Kallehauge TB, Hefzi H, Grav LM, 18. et al. Genome-scale reconstructions of the mammalian secretory pathway predict metabolic costs and limitations of protein secretion. Nat Commun. 2020; 11: 68. Available from: https://doi.org/10.1038/s41467-019-13867-y.
6. Kaste JAM, Shachar-Hill Y. Model validation and selection in metabolic 19. flux analysis and flux balance analysis. Biotechnol Prog. 2024; 40: e3413. Available from: https://doi.org/10.1002/btpr.3413.
7. Eisenhut P, Marx N, Borsi G, Papez M, Ruggeri C, Baumann M,
et al. Manipulating gene expression levels in mammalian cell 20. factories: An outline of synthetic molecular toolboxes to achieve multiplexed control. New Biotechnol. 2024; 79: 1-19. Available from: https://doi.org/10.1016/j.nbt.2023.11.003.
8. Kol S, Ley D, Wulff T, Decker M, Arnsdorf J, Schoffelen S, et al. 21. Multiplex secretome engineering enhances recombinant protein production and purity. Nat Commun. 2020; 11: 1908. Available from: https://doi.org/10.1038/s41467-020-15866-w.
9. Aviezer D, Brill-Almon E, Shaaltiel Y, Hashmueli S, Bartfeld D, Mizrachi S, et al. A Plant-Derived Recombinant Human 22. Glucocerebrosidase Enzyme — A Preclinical and Phase
I Investigation. PLOS ONE. 2009; 4: e4792. Available from: https://doi.org/10.1371/journal.pone.0004792.
10. Fausther-Bovendo H, Kobinger G. Plant-made vaccines and 23. therapeutics. Science 2021; 373: 740-1. Available from: https://doi.org/10.1126/science.abf5375.
11. Hellwig S, Drossard J, Twyman RM, Fischer R. Plant cell cultures
for the production of recombinant proteins. Nat Biotechnol. 2004; 24. 22: 1415-22. Available from: https://doi.org/10.1038/nbt1027.
12. Woo S-D, Roh JY, Choi JY, Jin BR. Propagation of Bombyx mori Nucleopolyhedrovirus in nonpermissive insect cell lines. J Microbiol Seoul Korea. 2007; 45: 133-8. 25.
13. Hong M, Li T, Xue W, Zhang S, Cui L, Wang H, et al. Genetic engineering of baculovirus-insect cell system to improve protein
production. Front Bioeng Biotechnol. 2022; 10. Available from: https://doi.org/10.3389/fbioe.2022.994743. Hu Y-C, Yao K, Wu T-Y. Baculovirus as an expression and/or delivery vehicle for vaccine antigens. Expert Rev Vaccines. 2008; 7: 36371. Available from: https://doi.org/10.1586/14760584.7.3.363. Fernandes B, Castro R, Bhoelan F, Bemelman D, Gomes RA, Costa J, et al. Insect Cells for High-Yield Production of SARS-CoV-2 Spike Protein: Building a Virosome-Based COVID-19 Vaccine Candidate. Pharmaceutics. 2022; 14: 854. Available from: https://doi.org/10.3390/pharmaceutics14040854. Struble LR, Smith AL, Lutz WE, Grubbs G, Sagar S, Bayles KW, et al. Insect cell expression and purification of recombinant SARS-COV-2 spike proteins that demonstrate ACE2 binding. Protein Sci. 2022; 31: e4300. Available from: https://doi.org/10.1002/pro.4300. Li T, Zheng Q, Yu H, Wu D, Xue W, Xiong H, et al. SARS-CoV-2 spike produced in insect cells elicits high neutralization titres in non-human primates. Emerg Microbes Infect. 2020; 9: 2076-90. Available from: https://doi.org/10.1080/22221751.2020.1821583. Fernandes B, Sousa M, Castro R, Schäfer A, Hauser J, Schulze K, et al. Scalable Process for High-Yield Production of PfCyRPA Using Insect Cells for Inclusion in a Malaria Virosome-Based Vaccine Candidate. Front Bioeng Biotechnol. 2022; 10. Available from: https://doi.org/10.3389/fbioe.2022.879078. Mabashi-Asazuma H, Kuo C-W, Khoo K-H, Jarvis DL. Modifying an Insect Cell N-Glycan Processing Pathway Using CRISPR-Cas Technology. ACS Chem Biol. 2015; 10: 2199-208. Available from: https://doi.org/10.1021/acschembio.5b00340. Strange K, Christensen M, Morrison R. Primary culture of Caenorhabditis elegans developing embryo cells for electrophysiological, cell biological and molecular studies. Nat Protoc. 2007; 2: 1003-12. Available from: https://doi.org/10.1038/nprot.2007.143.
Espejo L, Hull B, Chang LM, DeNicola D, Freitas S, Silbar V, et al. Long-Term Culture of Individual Caenorhabditis elegans on Solid Media for Longitudinal Fluorescence Monitoring and Aversive Interventions. JoVE J Vis Exp. 2022: e64682. Available from: https://doi.org/10.3791/64682.
Hobbs DJ, Fryer SE, Duimstra JR, Hedstrom OR, Brodie AE, Collodi PA, et al. Culture of Cells from Juvenile Worms of Schistosoma mansoni. J Parasitol. 1993; 79: 913-21. Available from: https://doi.org/10.2307/3283730.
Lazcano-Perez F, Roman-Gonzalez SA, Sanchez-Puig N, Espinosa RA-. Bioactive Peptides from Marine Organisms: A Short Overview. Protein Pept Lett. 2002; 19: 700-7. Available from: https://doi.org/10.2174/092986612800793208. Jayakrishnan A, Wan Rosli WR, Tahir ARM, Razak FSA, Kee PE, Ng HS, et al. Evolving Paradigms of Recombinant Protein Production in Pharmaceutical Industry: A Rigorous Review Sci. 2024; 6: 9. Available from: https://doi.org/10.3390/sci6010009. Cid R, Bolívar J. Platforms for Production of Protein-Based Vaccines: From Classical to Next-Generation Strategies. Biomolecules. 2021; 11: 1072. Available from: https://doi.org/10.3390/biom11081072.
