CC BY
31
62 • "PROTIST—2016
by the Russian Science Foundation, project 16-1410116.
HETEROTROPHIC FLAGELLATES OF SPHAGNUM BOGS IN SOUTH PATAGONIA, CHILE Prokina K.I.1, Mylnikov A.P.1, Philippov D.A.12
1 - Papanin Institute for Biology of Inland Waters, Russian Academy of Sciences, Borok, Nekouzsky District, Yaroslavl Oblast, 152742Russia
2 - Tyumen State Universsity, Tyumen, 630003Russia [email protected]
Bogs are habitats of great environment-modifying value, in particular, play a major role in the formation of the hydrological regime of the surrounding territory. A considerable amount of data has been accumulated on the heterotrophic flagellates associated with wetland ecosystems of the Northern Hemisphere while the large wetlands of the south part of South America remain almost unexplored. Species composition and morphology of heterotrophic flagellates collected from six sphagnum bogs in Chilean part of South Patagonia and Tierra del Fuego in October—November 2015 are described. Fifty-eight species from 33 genera and 3 macrotaxa (Opisthokonta, SAR, Excavata) and heterotrophic flagellates 'incertae sedis' have been recorded. Most of the recorded species are bacterivorous, 7 species — are omnivorous, and 1 — is a predator, feeding on other flagellates. In two bogs of continental part of Chile we have recorded 38 species and forms (38 species in bog no. 1; 1 species in drained bog no. 2), and in four bogs in Tierra del Fuego — we have recorded 39 species and forms (15 species in bog no. 3; 8 species in no. 4; 13 species in no. 5; 16 species in no. 6). Among all aquatic habitats associated with bogs, the greatest number of species has been recorded in hollows (35 species); bogs streams (25); small boggy lakes (24); pool near trunks of Nothofagus and peat excavation with cyanobacterial mat at the bottom (5); only 1 species has been recorded in a drainage canal. All identified flagellates are known from different types offreshwater habitats of the Northern Hemisphere, and most of them (31 species and 10 not identified to species level taxa are known from the bogs of the Northern Hemisphere.
This study was supported by the Russian Science Foundation (project no. 14-14-01134).
ATTRIBUTABLE RISK OF CAPILLARIA SPECIES IN DOMESTIC PIGEONS (COLUMBA LIVIA DOMESTICA) Qamar M.F.Q.12, Arifa Butt A.B.2
1 - University of Veterinary & Animal Sciences, Lahore
2 - Department of Zoology, GC University Lahore
Fecal samples were collected from 120 domestic pigeons to determine the Attributable risk of Ca-pillaria spp. The Capillaria spp. was observed in 64 out of 120 (51%) pigeons (70 males and 50 females) under study. A total of 64 (39 males and 25 females) were naturally infected with Capillaria spp. with 51% and 50% in males and females respectively. Qualitative examinations include the direct microscopy and faecal floatation while quantitative examination includes McMaster technique (worms are calculated per gram of the faeces). Month wise Attributable risk showed that eggs of the worms were found to be abundant in the month of July (60% to 73%) because of high humidity in the month of July during the present study. Very high and very low temperature is not suitable for the proper development of the eggs. Qualitative and quantitative examination revealed that Capillaria spp. was more prevalent in males (51%) than females (50%) but overall there is no significant difference (p>0.05) in the male and female because both of individuals invest equal amount of energy in search of food and incubating the eggs. Different breeds of the pigeons gave different Attributable risk in different months during the whole study. Group of pigeons from different locations showed variable Attributable risk. Areas with high humidity were more suitable for the development of eggs that is the reason that higher Attributable risk was observed in shahdara (75%).
NEBELA JIUHUENSIS NOV. SP. (AMOEBO-ZOA; ARCELLINIDA; HYALOSPHENIIDAE): A NEW MEMBER OF THE NEBELA SACCI-FERA - EQUICALCEUS - ANSATA GROUP DESCRIBED FROM SPHAGNUM PEAT-LANDS IN SOUTH-CENTRAL CHINA Qin Yangmin123, Man Baiying2, Anush Kosakyan4, Enrique Lara3, Gu Yansheng2, Wang Hongmei2, Zhang Wenwen12, Edward A.D. Mitchell56
1 - Department ofGeography, School ofEarth Science, China University of Geosciences, Wuhan, 430074, China
2 - State Key Laboratory of Biogeology and Environmental Geology, China University ofGeosciences, Wuhan, 430074, China
3 - Laboratory ofSoilBiology, University ofNeuchatel, Rue Emile Argand 11, CH-2000 Neuchatel, Switzerland
4 - Department of Zoology, Institute of Biosciences, University of Sao Paulo, Rua do Matao, travessa 14, Sso Paulo, Brazil
5 - Laboratory ofSoil Biology, University ofNeuchatel, Rue Emile Argand 11, CH-2000 Neuchatel, Swit-
Protistology ■ 63
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6 - Jardin Botanique de Neuchátel, Chemin du Per-thuis-du-Sault 58, CH-2000Neuchátel, Switzerland [email protected]
Hyalospheniids are among the most common and conspicuous testate amoebae in high-latitude peatlands and forest humus. These testate amoebae were widely studied as bioindicators, and are increasingly used as models in microbial biogeography. However, data on their diversity and ecology are still very unevenly distributed geographically: notably, data are lacking for low latitude peatlands. We describe here a new species, Nebela jiuhuensis, from peatlands near the Middle Yangtze River reach of south central China with characteristic morphology. The test (shell) has hollow horn-like lateral extensions also found in N. saccifera, N. equicalceus (=N. hippocrepis) and N. ansata, three large species restricted mostly to Sphagnum-peatlands of Eastern North America. Mitochondrial cytochrome oxidase (COI) data confirm that N. jiuhuensis is closely related to the morphologically very similar North American species N. saccifera and more distantly to N. ansata within the N. penardiana group. These species are all found in wet mosses growing in poor fens. Earlier re-ports ofmorphologically similar specimens found in South Korea peatlands suggest that N. jiuhuensis may be distributed in comparable peatlands in Eastern Asia (China and Korea). The discovery of such a conspicuous new species in Chinese peatlands suggests that many new testate amoebae species are yet to be discovered, including potential regional endemics. Furthermore, human activities (e.g. drainage, agriculture, pollution) have reduced the known habitat of N. jiuhuensis, which can thus be considered as locally endangered. We therefore suggest that this very conspicuous microorganism with a probably limited geographical distribution and specific habitat requirement should be considered as a flagship species for microbial biogeo-graphy as well as local environmental conservation and management. Key words: Arcellinid testate amoebae; biodiversity conservation; biogeography; DNA barcoding; mtCOI.
EPIBIONTIC RELATIONSHIP IN AN ARTIFICIAL POND OF CHIAPAS (MEXICO): PROCAMBARUS (AUSTROCAMBARUS) SP. (CRUSTACEA: DECAPODA) AND EPISTYLIS HENTSCHELI (CILIOPHORA: PERITRI-CHIA)
Ramírez-Ballesteros M.1, Fernandez-Leborans G.2, Mayén-Estrada R.3
1 - Laboratorio de Protozoología, Departamento de Biología Comparada, Facultad de Ciencias, Universidad Nacional Autónoma de México, Mexico
2 - Departamento de Zoología, Facultad de Biología, Universidad Complutense, Madrid, España
3 - Laboratorio de Protozoología, Departamento de Biología Comparada, Facultad de Ciencias, Universidad Nacional Autónoma de México, México [email protected]
Several samples collected during years 2014 and 2015 contained epibenthonic fauna with the crayfish Procambarus (Austrocambarus) sp. Samplings were performed on the freshwater artificial pond Yaleon, located in Montebello (Chiapas, SE Mexico), using a 5 mm opening hand net. The environment was strongly polluted with organic matter (assimilated to a polysaprobic zone). The crayfish Procambarus (Austrocambarus) sp. harbored colonies of a peri-trich ciliate identified as Epistylis hentscheli Kahl, 1935. This ciliate formed ramified colonies with a moderate number of zooids (up to 30). Stalks of the colony were dichotomously branched, and they presented peripherical fibres arranged longitudinally. The zooid was elongated; the form ofbody was similar to a bell (170-200 ^m in lenght), with a C-shaped transversal macronucleus, and a spherical micronucleus near to the macronucleus. The peristomal lip was very thin. The buccal infraciliature had the general pattern of peritrichids and was composed by a haplokinety and a polykinety drawing a spiral with 1.5 rounds inside the buccal infundibulum. These epibionts were located exclusively on the pereiopods. The goal of this contribution is to provide data about cell/colony structure of E. hentscheli and also its distribution on the crayfish. This is the first record ofthis epibiont peritrich ciliate on crustacea, being previously found on algae and fish. Acknowledgements: to Posgrado en Ciencias Biolygicas, Universidad Nacional Autónoma de México and CONACyT, for the support necessary for carry out this research. Additionally, we are indebted to Dr. Villalobos-Hiriart (Instituto de Biología, UNAM) for the crustacean identification and to Biol. Reyes-Santos (Facultad de Ciencias, UNAM) for their help with impregnation techniques. We also appreciate the technical support ofthe Department of Zoology (Universidad Complutense, UCM, España).
ORIGIN AND EVOLUTION OF PARAMECIUM BURSARIA / CHLORELLA SYMBIOTIC SYSTEM: WHAT CAN ONE SAY ABOUT THE HOST EVOLUTION STUDYING EVOLUTION OF SYMBIONT?
Rautian M., Beliavskaia A., Kiselev A. Saint Petersburg State University, Russia
