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1The 30th International Conference on Advanced Laser Technologies B-I-15
ALT'23
Laser-optic methods for determining the relationship of microrheologic properties of blood, microcirculation parameters and endothelium function of patients suffering from
socially important diseases
Priezzhev A.1, Lugovtsov A.1, Ermolinskiy P.1, Gurfinkel Yu.2, Diachenko P.3, Li Pengcheng4
1Faculty of Physics and 2Medical Research-Education Center ofM.V. Lomonosov Moscow State University,
Leninskie Gory 1/2, Moscow 119991, Russia 3N.G. Chernyshevsky Saratov State University, 83 ul. Astrakhanskaya, Saratov 410012, Russia 4Britton Chance Center for Biomedical Photonics and MoE Key Laboratory for Biomedical Photonics, Wuhan National Laboratory for Optoelectronics, Huazhong University of Science and Technology, China
avp2@mail.ru
During the last few years, we have been developing new methods of quantitative assessment of microrheologic properties of blood, microcirculation parameters and endothelium function (EF). These methods include diffuse light scattering, laser tweezers, digital capillaroscopy and tonometry [1-3]. Currently we work on combining these methods with laser speckle contrast imaging (LSCI) technique, which we use for mapping the blood flow speed so far in the laboratory small animals [4-5]. Microcirculation parameters are studied using the Kapilyaroscan-1 device (Russia) implementing artificial intelligence for digital image processing, which allows for estimating the number of red blood cells (RBC) in the capillaries [6]. Arterial stiffness and EF are determined by pulse tonometry using the Angiochek device (Russia). We characterize the microrheologic properties of blood by the intrinsic properties of RBC to reversibly aggregate and to deform in shear flow by measuring such parameters as aggregation index, aggregation rate, hydrodynamic strength of aggregates, paired aggregation and disaggregation forces, RBC deformability indices as functions of shear stress [7]. These measurements are performed in vitro in the samples of blood freshly drawn from healthy donors or patients suffering from socially important diseases. Digital capillaroscopy, tonometry and LSCI measurements are performed in vivo. Three groups of patients were formed for the study with the main diagnoses of atrial fibrillation (AF), coronary heart disease (CHD), and chronic heart failure (CHF). Statistical differences in parameters for different groups of patients were analyzed using the nonparametric statistical Mann-Whitney test (p<0.05). The correlation between capillary blood velocity (CBV) with the number of RBC aggregates in the capillaries was calculated according to the Pearson coefficient of linear correlation. The results of the study showed a statistically significant correlation of measured parameters for the AF and CHF groups of patients. In percentage terms, the ratio of CBV in capillaries without aggregates to CBV in capillaries with aggregates for different groups of patients equals to 39.6% (AF); 46.9% (CHD); 46.4% (CHF). Based on these data we can conclude that there is a significant correlation between the presence of aggregates in the capillaries and a decrease in CBV in the examined patients. The analysis of the relationship between the number of aggregates in capillaries and EF in patients suffering from AF and CHD has showed that the impairment of EF is associated with an increase in the number of RBC aggregates in the capillaries of patients of both groups.
The study was financially supported by the Russian Science Foundation grant № 23-45-00027.
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