CC BY
19
abstracts. phytopharm 2012
CRS) and aspirin (4h ASP) induced ulceration. The ulcer index and % protection was calculated. The gastric juice collected from 4h PL rats was studied for the effects on gastric volume, pH, free acid, total acid-pepsin output, DNA gastric juice cell shedding and mucosal cell proliferation. Biochemical estimation such as histamine content, mucus content and H+K+ATPase inhibitory activity was also evaluated. The effect of APF on stomach histopathology was also studied. Statistical analysis was done by one way ANOVA followed by Dun-nett's post hoc test for multiple comparisons against control group. The difference was considered to be significant when P < 0.05. Oral treatment of APF (200 and 400 mg/kg, p. o.) for 7 days showed significant protection against acute gastric ulcer induced by 4h PL
and 95 % EtOH, but was found to have no effect on CRS and ASP-induced gastric ulceration. The gastric studies showed that treatment with APF signi cantly increased the gastric juice pH, decrease the gastric volume and total acid-pepsin output and significantly reduced the gastric juice cell shedding. A significant decrease in histamine content and a reduction in free phosphate ions responsible for H+K+ATPase inhibitory activity were also observed. in conclusion, the results of the present study indicate that the gastroprotective activity of APF contributes mainly to the peripheral effects on the gastric secretion and the probable mechanism underlying behind such activity might be both due to the possible inhibition on the histaminic (H2) receptor and H+K+ATPase pump.
INVESTIGATION OF THREE TRITERPENE
ACIDS AGAINST CANCER CELL PROLIFERATION ACTIVITY
© Khoo Gaik Ming, Clausen Morten Rahr, Larsen Erik
Department of Food Science, Aarhus University, Kirstinebjergvej 10, DK-5792 Aarslev, Denmark
The object of this study was to investigate the inhibitory effect of betulinic acid, oleanolic acid and ursolic acid on human colon cancer cell proliferation activity. Triterpene acids are members of the terpene family and are widely distributed in food plants. A rosehip fraction containing a mixture of betulinic acid, oleanolic acid and ursolic acid displayed inhibitory effect against the release of pro-inflammatory cytokine interleukin-6 with the combination of oleanolic acid/betulinic acid and ursolic acid/betulinic acid displaying significant synergistic inhibitory effects (1). Our results showed that these three compounds also displayed good inhibitory effect against cancer cell proliferation activity at the concentration of 10^g/ml. both betulinic acid and ursolic acid displayed strong inhibition against HT-29 (88 %o and 79 <%, respectively) and SW-480 (76 <% and 82 %, respectively), but a moderate to weak inhibition against Caco-2 (19 % and 22 %, respectively). Oleanolic acid displayed only moderate inhibition against Caco-2 (49 %), very weak inhibition against HT-29 (7 %) and
no inhibition against SW-480. No synergistic effects were observed between combinations of the triterpene acids. We only found an additive effect in the combination of oleanolic acid/ursolic acid and only against proliferation of HT-29. Antagonistic effects between the pure compounds were observed when betulinic acid was one of the components in the combination. Although betulinic acid and ursolic acid showed good inhibitory effects against all three cancer cell lines, their combination displayed antagonistic effects and showed poor inhibition against cancer cell proliferations. However, these triterpenic acids and their combination effects should be further investigated in different in vitro or in vivo experiment to confirm their bioactivities and mechanism.
Reference: (1) Saaby L., Moesby L., Hansen E. W., Christensen S. B. (2011). isolation of immunomodulatory triterpene acids from a standardized rose hip powder (Rosa canina L.). Phytotherapy Research, 25, 195-201.
DECURSIN AND RELATED COMPOUNDS INHIBIT VASCULAR ENDOTHELIAL MIGRATION BY SPHINGOSINE KINASE INHIBITION
© Lee Yong-Moon, Shin Kyong-Oh, Seo Cho-Hee, Kim Tack-Joong
College of Pharmacy, Chungbuk National University, Cheongju, South Korea 2Division of Biological Science and Technology, Yonsei University, Wonju, South Korea
The pyranocoumarin compound decursin isolat- little is known about their anti-angiogenic activity. ed from the herb, Angelica gigas, are known to pos- Here, we investigated the anti-angiogenic effects of sess potent anti-inflammatory activities. However, decursin by the changes of sphingolipid metabolites
fM 76 ® Obzory po kliniceskoj farmacologii i lekarstvennoj terapii [Reviews of clinical pharmacology and drug therapy]
TOM 10/2012/2
