CC BY
7B-I-1
BIOMEDICAL PHOTONICS
In vivo tissue optical clearing as a tool for laser diagnostics
and therapeutics
V.V. Tuchin
Science Medical Center, Saratov State University, Astrakhanskaya str. 83, Saratov, 410012, Russia Laboratory of Laser Molecular Imaging and Machine Learning Tomsk State University, Lenin's av. 36, Tomsk,
634050, Russia
Laboratory of Laser Diagnostics of Technical and Living Systems, Institute of Precision Mechanics and Control, FRC "Saratov Research Centre of Russian Academy of Sciences," Rabochaya str. 24, Saratov, 410028, Russia
e-mail: tuchinvv@mail.ru
The principles and advances in the field of tissue optical clearing, as well as a discussion of new areas of biomedical applications of laser technologies, in particular for multimodal imaging, monitoring of drug delivery and laser therapy are presented [1-3]. Tissue optical clearing is based on the control of the optical properties of tissues by reducing light scattering and improvement of scattering anisotropy factor due to administration of optical immersion agents. The main mechanisms of optical clearing are matching of the refractive indices of tissue components and tissue dehydration with a high impact of constructive interference of scattered waves during temporary and reversible modification of tissue morphology caused by redistribution of refractive index and thicknesses of tissue layers.
Problems of in vivo delivery of optical clearing agents, creation of virtual tissue optical windows from UV to THz, multimodal imaging using MRT and CT contrast agents will be discussed. Benefits of a number of laser techniques such as Raman tissue spectroscopy, photoacoustic lymph- and angiography, laser-induced breakdown spectroscopy (LIBS) at using optical clearing will be demonstrated. Laser and optical techniques used for quantification of tissue glycation with the help of diffusion of probing molecules of optical clearing agents in healthy and glycated tissues will be presented. It will be shown that tissue optical clearing is prospective for anticancer and antimicrobial/antifungal/antiviral photody-namic and photothermal therapies.
1. L. Oliveira and V. V. Tuchin, The Optical Clearing Method: A New Tool for Clinical Practice and Biomedical Engineering, Springer Nature Switzerland AG, Basel, 2019 - 177 p.
2. V V Tuchin, D. Zhu, and E. A. Genina (Eds.), Handbook of Tissue Optical Clearing: New Prospects in Optical Imaging, Taylor & Francis Group LLC, CRC Press, Boca Raton, FL, 2022 - 688 p.
3. VV Tuchin, E.A. Genina, E.S. Tuchina, A.V. Svetlakova, Y.I. Svenskaya, Optical clearing of tissues: issues of antimicrobial phototherapy and drug delivery, Advanced Drug Delivery Reviews 180 (1), 114037 (2022).
ALT'22
