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4*
ALT'23 The 30th International Conference on Advanced Laser Technologies
B-O-9
Hybrids of carbon quantum dots and photoswitchabl phosphonates -peculiarities of optical and biological properties
Gulia Bikbaeva14, Anna Pilip2, Anastasia Egorova2'3, Ilya Kolesnikov4, Dmitry Pankin4, Kirill Laptinskii56, Alexey Vervald6, Tatiana Dolenko6 and Alina Manshina1
1-Institute of Chemistry, St. Petersburg State University, St. Petersburg 198504, Russia 2- St. Petersburg Federal Research Center of the Russian Academy of Sciences (SPC RAS), Scientific Research Centre for Ecological Safety of the Russian Academy of Sciences, St. Petersburg 197110, Russia 3- St. Petersburg State Technological Institute (Technical University), St. Petersburg 190013, Russia 4- Center for Optical and Laser Materials Research, St. Petersburg State University, St. Petersburg, Russia 5- Faculty of Physics, M.V Lomonosov Moscow State University, Moscow 119991, Russia 6- Skobeltsyn Institute of Nuclear Physics, M.V. Lomonosov Moscow State University (SINP MSU), Moscow 119991,
Russia
*e-mail: [email protected]
Nowadays the Photopharcology is an urgent area of research. The main players of photopharmacology are 'photoswitch-pharmacore' couples that change biological activity because of light irradiation. Photopharmacological agents of a new generation can be used to treat various diseases, including Alzheimer's disease [1]. It is important, that practical implementation of photopharmacology is closely related with spatial control of medicinal treatment zone. Thus, demonstration of substances that meet all the listed requirements would determine the directions of breakthrough research in the coming years.
The purpose of this study is to develop photopharmacological agents that combine a number of functions - biological activity, the ability to change it under the influence of light and visualization of the zone of drug exposure.
In this study, we present for the first time CQDs@phosphonate nanohybrid that is conjunction of biocompatible and nontoxic luminescent carbon quantum dots (CQDs) with photoswitchable phosphonate compound possessing inhibition of butyrylcholinesterase (BChE) - prognostic marker of numerous diseases [2]. The study of the obtained nanohybrids using absorption spectroscopy, luminescence, FTIR spectroscopy and IPC -micro neurotoxins amperometric analysis revealed a pronounced effect of laser radiation on the optical and biological properties of new objects. CQDs@phosphonate hybrids demonstrate step increase of butyrylcholinesterase inhibition from 38% up to almost 100% and simultaneous luminescence decrease after laser irradiation with wavelength 266 nm. The all the listed Hybrids properties were demonstrated not only for in vitro experiments, but for complex biological sample - chicken breast. The uncovered photosensitivity and bioactivity of new phosphorylated phosphonates makes them promising compounds for clinical therapy as photopharmacological agents.
Acknowledgements
This work was supported by RSF project 22-13-00082. Authors are grateful to "Centre for Optical and Laser materials research", Research Park of Saint Petersburg State University for technical support. Authors are grateful to Interdisciplinary Scientific and Educational School of Lomonosov Moscow State University "Photonic and Quantum technologies. Digital medicine".
References
[1] I. Kolesnikov, D. Mamonova, D. Pankin, G. Bikbaeva, A. Khokhlova, A. Pilip, A. Egorova, V. Zigel, A. Manshina, Photochemistry and Photobiology. 99(3), 929-935(2023)
[2] I. Kolesnikov, A. Khokhlova, D. Pankin, A. Pilip, A. Egorova, V. Zigel, M. Gureev, G. Leuchs, A. Manshina, New J. Chem. 45, 15195-15199(2021)
