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SYNTHETIC
ANTIBODIES
FOR CUNICAL USE
REGULATING TELOMERASE IN ONCOGENESIS
R Bl Y% A v
THE STRUCTURE
of the Mitochondrial genome as AN ACTIVATOR OF OPISTHORCHIASIS
— V\\
STACKING INTERACTIONS IN COMPLEXES'OF FIBERS WITH ADENINE-AND
■* *4®
ActaNaturae I
hemh Hi
HIGHLIGHTS
K.G. Skryabin, E.B. Prokhortchouk, A.M. Mazur, E.S. Boulygina, S.V. Tsygankova, A.V. Nedoluzhko, S.M. Rastorguev, V.B. Matveev, N.N. Chekanov, D.A. Goranskaya, A.B. Teslyuk, N.M. Gruzdeva, V.E. Velikhov, D.G. Zaridze, M.V. Kovalchuk
Combining Two Technologies for Full Genome Sequencing of Human
The study is the first Russian effort towards whole human genome sequencing.
In this study, the whole genome sequencing of a human (Russian man) was performed using two technologies currently presented on the market - Sequencing by Oligonucleotide Ligation and Detection (SOLiDTM) and sequencing technologies of molecular clusters using fluorescently labeled precursors. Optimal algorithm of using the latest methods of sequencing was established for analysis of individual human genomes
100 200 300 400 500 600 700
900 1000 1100 1200 1300 1400 1500
Insert size, bp
Graph of distances distribution between pairs of reads for the human reference genome ver. hg18 libraries
A schematic representation of signal transduction via JAK-associated membrane receptors
D.G. Knorre, N.V. Kudryashova, T.S. Godovikova
Chemical and Functional Aspects of Posttranslational Modification of Proteins
This paper reviews the chemical and functional aspects of the posttranslational modifications of proteins, which are achieved by the addition of various groups to the side chain of the amino acid residue backbone of proteins. Much attention is paid to the role of posttranslational modification of proteins in the regulation of biochemical processes in live organisms
T.V. Rakitina, O.V. Yudkina, E.V. Smirnova, A.V. Lipkin
Protein Tyrosine Kinase Panel As a Tool for Anticancer Drug Design
This work is an example of a combination of inhibitor experimental search with the computational analysis of the potential mechanism of the inhibitors’ action, which allowed to propose the 2-hydroxyphenol group as a scaffold for the design of new tyrosine kinase inhibitors.
Abl, Blk, FGFR1, PDGFRa, Yes Blk, Csk, EphA2, FGFR1, FGFR2, Lyn ON mo
Abl
FGFR1
FGFR2
PDGFRa
°
\ /"
/ V.
FGFR2 •>. Lyn
PDGFRa
Abl, Bl, Csk, EphA2, FGFR1 Abl, Blk, FGFR1, FGFR2, Lyn, PDGFRa, Pyk2 FGFR2, GFR1, Lyn PDGFRa
The small molecule tyrosine kinase inhibitors that were found by screening of the chemical collection
RNA
cDNA
PCR of the VH-and VL-genes
J3.
F-—
Insertion into the phagemid
Transformation .... ' into E. coli ■ ■
Amplification of phage particles
§
N.V. Tikunova, V.V. Morozova
Phage Display on the Base of Filamentous Bacteriophages: Application for Recombinant Antibodies Selection
The display of peptides and proteins on the surface of filamentous bacteriophage is a powerful methodology for selection of peptides and protein domains, including antibodies. An advantage of this methodology is the direct physical link between the phenotype and the genotype, as an analyzed polypeptide and its encoding DNA fragment exist in one phage particle. This review is an introduction to phage display methodology. It presents recombinant antibodies display in more details
A schematic representation of the construction of an antibody fragment phage library
Blk
. EphA2
FGFR1
Ap
E.coli TG1
№ 3 2009 | ACTA NATURAE | 3
