CC BY
7HIGH SPEED PHOTOACOUSTIC IMAGING CHENGBO LIU
Shenzhen Institute of Advanced Technology, Chinese Academy of Sciences, China
cb.liu@siat.ac.cn
ABSTRACT
Photoacoustic imaging possesses the merits of both high optical contrast and large acoustic penetration. It is expected to play a more and more important role in both fundamental research and clinical applications, and has the potential to revolutionize the playground of tumor and cardiovascular disease management and treatment. In this talk, I will focus on how we improve the speed of photoacoustic imaging. I will start with conventional photoacoustic microscopy using point-by-point mechanical scanning. Then I will move on to fast optical scanning based on MEMS and polygon mirror devices. After that, I will introduce single pixel imaging method without the need of scanning. Combined with sparse sampling, the imaging speed can be improved by 20 times. Besides photoacoustic microscopy, we also built photoacoustic computed tomography system which uses an array of ultrasound transducers to sense the signals. Due to simultaneous acquisition of photoacoustic signals, PACT can acquire 100 frames of two-dimensional images in one second. We applied our technology and instrumentations to study tumor microenvironment and evolution of tumor, as well as to investigate the mechanism of neurovascular coupling in the brain. We also intraoperatively guided a biopsy needle with photoacoustic imaging to extract tissue samples from sentinel lymph node to monitor tumor metastasis status. Our technology has also been successfully translated to human imaging to diagnose vascular plaques and image periphery blood vessels, aiming at better management of diseases such as diabetic foot or varicosity.
Figure 1: Photoacoustic imaging of microvessels at different organ sites
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Figure 2: Photoacoustic imaging of calf gastrocnemius region offive human volunteers, M: male, F: female
REFERENCES
[1] Ningbo Chen, Jia Yu, Liangjian Liu, Zhiqiang Xu, Rongkang Gao, Tao Chen, Liang Song, Wei Zheng*, Chengbo Liu, "Video-rate high-resolution single-pixel nonscanning photoacoustic microscopy, Biomedical Optics Express. 3823-3835, 2022.
[2] Zhiqiang Xu, Yinhao Pan, Ningbo Chen, Silue Zeng, Liangjian Liu, Rongkang Gao, Jianhui Zhang, Chihua Fang, Liang Song, Chengbo Liu, "Visualizing tumor angiogenesis and boundary with polygon-scanning multiscale photoacoustic microscopy, Photoacoustics. 100342, 2022.
[3] Tao Chen, Liangjian Liu, Xiaohui Ma, Ying Zhang, Huadong Liu, Rongqin Zheng, Jie Ren, Huichao Zhou, Yaguang Ren, Rongkang Gao, Ningbo Chen, Hairong Zheng, Liang Song, Chengbo Liu, Dedicated photoacoustic imaging instrument for human periphery blood vessels: A new paradigm for understanding the vascular health, IEEE Transactions on Biomedical Engineering. 1093-1100, 2022.
[4] Huangxuan Zhao, Ziwen Ke, Fan Yang, Ke Li, Ningbo Chen, Liang Song, Chuansheng Zheng, Dong Liang, and Chengbo Liu, Deep Learning Enables Superior Photoacoustic Imaging at Ultralow Laser Dosages, Advanced Science. 2003097, 2021.
[5] Huangxuan Zhao, Ningbo Chen, Tan Li, Jianhui Zhang, Riqiang Lin, Xiaojing Gong, Liang Song, Kunya Zhang, Zhi Yang, Zhicheng Liu, Chengbo Liu, Motion Correction in Optical Resolution Photoacoustic Microscopy, IEEE Transactions on Medical Imaging. 2139-2150, 2019.
