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6GAP-ENHANCED RESONANCE RAMAN TAGS FOR BIOIMAGING
JIAN YE
School of Biomedical Engineering, Shanghai Jiao Tong University, P.R. China
yejian78@sjtu.edu.cn
Abstract
Gap-enhanced Raman tags (GERTs) are emerging probes of surface-enhanced Raman scattering(SERS) spectroscopy that have found promising analytical, bioimaging, and theranostic applications [1-5].Because of their internal location, Raman reporter molecules are protected from unwantedexternal environments and particle aggregation and demonstrate superior SERS responses owing tothe strongly enhanced electromagnetic fields in the gaps between metal core-shell structures. However, long-term live-cell Raman bioimaging is limited due to the photodamage from a relatively long exposure time and a high laser power, which are needed for acquiring detectable Raman signals.
In thistalk, we will briefly discuss recent progress in the synthesis, simulation, and experimental studies of theoptical properties and biomedical applications of novel spherically symmetrical and anisotropicGERTs fabricated with common plasmonic metals—gold (Au) and silver (Ag).Next, we attempt to resolve this photodamage issue by developing ultrabright gap-enhanced resonance Raman tags (GERRTs), consisting of the petal-like Au core and Ag shell with near-infrared resonant reporters of IR-780 embedded in between, for long-term and high-speed live-cell imaging [6]. GERRTs exhibit the ultrahigh Raman intensity down to a single-nanoparticle level in the aqueous solution and solid state upon 785 nm excitation, allowing for high-resolution time-lapse live-cell Raman imaging with an exposure time of 1 ms per pixel and a laser power of 50 ^W. Under this measurement condition, we can possibly capture dynamic cellular processes with a high temporal resolution, and track living cells for long periods of time owing to the reduced photodamage to cells. These nanotags open new opportunities for ultrasensitive, low-phototoxic, and long-term live-cell imaging.
[1] L. Lin, M. Zapata, M. Xiong, Z. Liu, S. Wang, H. Xu, A. G. Borisov, H. Gu, P. Nordlander, J. Aizpurua, J. Ye.Nanooptics of plasmonic nanomatryoshkas: shrinking the size of a core-shell junction to subnanometer, Nano Letters. 15, 6419-6428, 2015
[2] Z. Bao, Y. Zhang, Z. Tan, X. Yin, W. Di, J. YeGap-enhanced Raman tags for high-contrast sentinel lymph node imaging, Biomaterials, 163, 105-115, 2018.
[3] Y. Qiu, Y. Zhang, M. Li, G. Chen, C. Fan, K. Cui, J. Wan, A. Han, J. Ye, Z. Xiao, Intraoperative Detection and Eradication of Residual Microtumors with Gap-Enhanced Raman Tags,ACS Nano, 12, 7974-7985, 2018.
[4] Y. Zhang, Y. Gu, J. He, B. D. Thackray, J. Ye.Ultrabright gap-enhanced Raman tags for high-speed bioimaging,Nature Communications, 10, 3905,2019.
[5] Y. Gu, C. He, Y. Zhang, L. Lin, B. D. Thackray, J. Ye.Gap-enhanced Raman tags for physically unclonable anticounterfeiting labels,Nature Communications, 11, 516,2020.
[6] Y. Gu, X. Bi,J. Ye.Gap-enhanced resonance Raman tags for live-cell imaging,J. of Mater. Chem. B,8, 6944-6955, 2020
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Figure: (a) Scheme of a GERRT, and (b) GERRTs-based live cell bioimaging;
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