Experimental study of dendrimer-based nanoparticles with RGD-peptide for anticancer radionuclide therapy

Stukalov Yuv; Grigorieva Eyu; Smirnova Av; Lipengolts Aa; Kubasova Iyu; Pozdniakova Nv; Lukashina Mi

EXPERIMENTAL STUDY OF DENDRIMER-BASED NANOPARTICLES WITH RGD-PEPTIDE FOR ANTICANCER RADIONUCLIDE THERAPY

Stukalov YuV1, Grigorieva EYu1 и, Smirnova AV1*3, Lipengolts AA1>2, Kubasova IYu1, Pozdniakova NV1, Lukashina MI4

1 Blokhin National Medical Research Center of Oncology, Moscow

2 Burnazyan Federal Medical Biophysical Center, Moscow

3 Loginov Moscow Clinical Scientific Center, Moscow

4 Dmitry Rogachev National Research Center of Pediatric Hematology, Oncology and Immunology, Moscow

Radionuclide therapy (RNT) is an effective modality for treating multiple metastases in patients with cancer. The list of malignancies that can be managed with RNT expands with the arrival of novel tumoritropic radiopharmaceuticals (RP). A versatile delivery platform capable of carrying various therapeutic and diagnostic radionuclides, as well as vector molecules needed to achieve sufficient specificity to tumor cells and ensure therapeutic efficacy may hold great promise for radiation therapy. The aim of this work was to assess the performance of a delivery system based on the original dendrimer. The dendrimer demonstrated low toxicity in mice (LD50 was 779 ± 111 mg/kg). To study the specificity of the dendrimer to tumor cells and its therapeutic efficacy, we used a nanoplatform (NP) composed of the dendrimer itself, the RGD peptide and 188Re (188Re-NP). Lewis lung carcinoma LLC1 was used as a tumor model. The biodistribution analysis revealed that the compound effectively accumulated in the tumor demonstrating a tumor-to-normal ratio >1 (relative to healthy organs and tissues) and retention time of at least 6 hours. Injections of 185 MBq/kg 188Re-NP caused a statistically significant inhibition of tumor growth (p < 0.05) by day 7 following the injection (Т/С = 5%), which remained stable for 6 days. Our findings suggest that the proposed dendrimer is a promising platform for RP delivery.

Keywords: dendrimer, RGD peptide, 188Re, radionuclide therapy, biodistribution, research in animals, transplanted tumor model

[X] Correspondence should be addressed: Elena Yu. Grigorieva Kashirskoe shosse 24, Moscow, 115478; grig-elen11@mail.ru

Received: 12.09.2018 Accepted: 11.10.2018

DOI: 10.24075/brsmu.2018.089

ЭКСПЕРИМЕНТАЛЬНЫЕ ИССЛЕДОВАНИЯ ДЕНДРИМЕРНОЙ НАНОКОНСТРУКЦИИ С RGD-ПЕПТИДОМ ДЛЯ РАДИОНУКЛИДНОЙ ТЕРАПИИ ОНКОЛОГИЧЕСКИХ ЗАБОЛЕВАНИЙ

Ю. В. Стукалов1, Е. Ю. Григорьева1 А. В. Смирнова1,3, А. А. Липенгольц1,2, И. Ю. Кубасова1, Н. В. Позднякова1, М. И. Лукашина4

1 Национальный медицинский исследовательский центр онкологии имени Н. Н. Блохина, Москва

2 Федеральный медицинский биофизический центр имени А. И. Бурназяна, Москва

3 Московский клинический научно-практический центр имени А. С. Логинова, Москва

4 Национальный медицинский исследовательский центр детской гематологии, онкологии и иммунологии имени Дмитрия Рогачева, Москва

Радионуклидная терапия (РНТ) является эффективным методом лечения множественных метастазов злокачественных опухолей. Расширение номенклатуры злокачественных новообразований, для которых возможно применение РНТ, происходит за счет создания новых туморотропных радиофармацевтических препаратов (РФП). Перспективно создание РФП на основе универсальной транспортной платформы, которая может быть модифицирована различными терапевтическими и диагностическими радионуклидами, а также векторными молекулами для достижения требуемой специфичности к опухолям и терапевтической эффективности. Целью работы было оценить в качестве такой транспортной платформы конструкцию на основе оригинального дендримера. Исследование на мышах показало его низкую токсичность (ЛД50 достигало 779 ± 111 мг/кг). Туморотропность и терапевтическую эффективность дендримера исследовали на примере наноконструкции (НК) из дендримера, RGD-пептида и радионуклида 188Re (^Re-НК). В качестве опухолевой модели использовали мышиную карциному легкого Льюиса LLC1. Данные биораспределения предложенной НК показали ее эффективное накопление в опухоли с коэффициентом дифференциального накопления более 1 по отношению к основным органам и тканям и временем удержания в опухоли не менее 6 ч. Введение ^Re-НК в дозе 185 МБк/кг мышам с подкожно трансплантированной опухолью статистически достоверно (p < 0,05) способствовало торможению роста опухоли к 7-м суткам после введения до Т/С = 5%, сохраняющемуся в течение 6 суток. Проведенные исследования показали перспективность исследованного дендримера как транспортной платформы для РНТ.

Ключевые слова: дендример, RGD-пептид, 188Re, радионуклидная терапия, биораспределение, исследование на животных, перевивные опухолевые модели

Для корреспонденции: Елена Юрьевна Григорьева Каширское шоссе, д. 24, г Москва, 115478; grig-elen11@mail.ru

Статья получена: 12.09.2018 Статья принята к печати: 11.10.2018

DOI: 10.24075/vrgmu.2018.089

The use of drugs that can effectively and selectively accumulate in malignant tissue is a key to the success of radionuclide, neutron capture and photon activation therapies [1]. Recently, there has been a burgeoning interest in dendrimers [2-6], the spherical molecules sized 2-10 nm that have a large number of functional groups in their outer shell and, therefore, can be conjugated to a wide range of different molecules. This facilitates creation of targeted drug delivery platforms by attaching a tumor-specific agent and a tumoricidal or a diagnostic agent to the dendrimer. Previously, we demonstrated the feasibility and promise of this approach for the therapy and diagnosis of cancer [7-8]. Such platforms can exploit the affinity of cancer cell receptors to a number of low molecular weight compounds. For example, dendrimers functionalized with p-estradiol have been shown to effectively accumulate in transplanted breast adenocarcinoma cells (Ca755) [9]. In the study below, the role of a target-specific component of a tested dendrimer-based platform was played by the RGD-peptide capable of biding to the integrins present on the surface of cancer cells [10-17].

This work aimed to investigate the feasibility of using the original dendrimer-based nanoplatform in radiation therapy and cancer diagnostics in a series of in vivo experiments.

METHODS

The original nanoplatform (NP) consisted of a first-generation dendrimer covalently conjugated to safranin, which binds sodium perrhenate, and a tumor-specific RGD peptide. The dendrimer itself was previously described in [7]. Fig. 1 shows the NP; its structure was confirmed by nuclear magnetic resonance spectroscopy.

The radionuclide 188Re with a half-life of 17 hours was used as a therapeutic agent. Its decay is accompanied by the emission of p-radiation with energies of 2.12 MeV producing the tumoricidal effect and y-radiation with energies of 155 keV (15.2%) detectable by a y-camera that records distribution of a radiopharmaceutical agent in a patient's body 18-23]. The GREN-1 188W/188Re generator (Leipunsky Institute of Physics and Power Engineering, Russia) used in this study was generating 188Re over the course of 4-6 months [24]; therefore, the studied compounds could be labeled with 188Re immediately before use.

Radiolabeling was performed by combining the studied NP and 188Re sodium perrhenate eluted from the generator. The amount of 188RE-NP in the working solution was calculated based on the molarity of the introduced 188Re (1 MBq 188Re — 0.00015 nM). To make sure every 188Re was bound, NP were taken at 100-fold excess. The isotope was added to the studied compounds ex tempore. Nuclear magnetic resonance spectra were recorded by the WH-360 spectrometer (Bruker; Germany) operating at 360 MHz. Thin-layer chromatography was performed using Si 60-coated plates (particle size of 5-17 pm) (Fluka; USA). Chromatograms were developed in iodine vapor.

Due to the high costs of the RGD peptide, acute toxicity of 188RE-NP was inferred from the toxicity of the unmodified dendrimer measured in healthy male Balb/c mice weighing 19-21 g. (Stolbovaya nursery of the Research Center for Biomedical Technologies, Russia). The experiments were conducted in full compliance with ethical principles and guidelines for animal research [25].

The animals were kept in the conventional vivarium under natural light conditions. For the experiment, the mice were distributed into 10 groups of 6. Each group consisted of animals of the same age. The dendrimer was dissolved in 0.9% NaCl solution containing 10% of DMSO. The animals received

a single intraperitoneal injection of 0.2 ml of the dendrimer solution. In total, ten different dendrimer doses were tested for toxicity: 62.5 mg/kg; 125 mg/kg; 187.5 mg/kg; 250 mg/kg; 375 mg/kg; 500 mg/kg; 750 mg/kg; 1,000 mg/kg; 1,250 mg/kg; and 1,500 mg/kg. The general health of mice and their behavior were monitored for 30 days following the injection. All changes were recorded on a daily basis. The animals who did not survive the experiment were necropsied and their internal organs were examined. Thirty days after the injection, the rest of the mice were euthanized by cervical dislocation. Acute toxicity of the studied compound was assessed based on the number of animals who died during the experiment, the day of their death, clinical manifestations of the intoxication, changes in behavior, and macroscopic examination of the organs and tissues conducted post-mortem [26-28]. Toxic doses were calculated in BioStat Pro 2008 5.0.1 (AnalystSoft; USA).

Biodistribution of the synthesized 188RE-NP was compared to that of 188Re sodium perrhenate in animals with subcutaneously transplanted LLC1 cells from the collection of cancer cell lines of Blokhin National Medical Research Center of Oncology, Russia. The choice of the cell line was dictated by the fact that the avp3 receptor, which is an RGD-binding integrin, has been reported to homogenously distribute in the LLC1 tumor [29]. Male C57Bl/6 mice weighing 19-21 g were divided into groups of 6. The suspension of cancer cells (4, 000, 000 cells per animal) was transplanted subcutaneously in the right thigh of each mouse. On day 10 following the inoculation, the mice received 0.2 ml of 188RE-NP (1.85 MBq per mouse, or 92.5 MBq/kg) injected intravenously. The mice were decapitated 1, 3, 6, 9, 13, and 24 h after the 188RE-NP injection. Tissue and arterial blood samples were collected during autopsy. Radioactivity of the injected doses was determined using the dose calibrator ISOMED 2010 (MED Nuklear-Medizintechnik Dresden GmbH; Germany). Distribution of 188RE-NP in the biological tissue of mice was studied by direct radiometric measurements. The emitted radiation was measured by WIZARD 2480 scintillation

Fig. 1. The nanoplatform based on the dendrimer conjugated to the RGD peptide

gamma-counter (Perkin Elmer; USA). 188RE-NP accumulation was evaluated based on the amount of 188Re in 1 g of the tissue/organ relative to its injected amount.

The therapeutic efficacy of 188RE-NP was tested in male С57В1/6 mice weighing 20-22 g. The mice were distributed into groups of 8. Two days after the subcutaneous transplantation of the LLC1 cells, the mice received single 0.2 ml doses of 188Re sodium perrhenate and 188RE-NP in 0.9% NaCl solution. The following 188Re doses were studied for their therapeutic effect: 15 MBq/kg, 92.5 MBq/kg, and 185 MBq/kg (0.3 MBq, 1.85 MBq, and 3.7 MBq per animal, respectively). The control group received 0.2 ml of 0.9% NaCl solution. The size and volume of tumors were measured on a daily basis throughout the experiment. The T/C value (a standard indicator of a tumoricidal effect) was calculated for the control and experimental groups using the equation [30]:

Т/С = 100 • V / V t,

exper contr

where V is an average tumor volume in the experimental

exper ^ r

group and V , is an average tumor volume in the control

^ r contr ^

group.

The data were analyzed in OriginPro 8.0 (OriginLab; USA) and Excel 2003 (Microsoft; USA). Statistical significance of the obtained results was tested using the nonparametric MannWhitney U test; differences were considered significant at p < 0.05.

RESULTS

The acute toxicity of the studied compound was assessed based on the number of animals who did not survive the experiment and the day of their death following the dendrimer injection. We found that the lowest lethal dose of the dendrimer was 500 mg/kg; it killed 2 of 6 animals. Four of six mice died at a dose of 1,000 mg/kg. A dose of 1,500 mg/kg was lethal

Table 1. Dynamics of 188Re-NP and 188Re accumulation in the organs and tissues of mice with subcutaneously transplanted LLC1 cells (expressed as % from the Injected amount per 1 g of the organ/tissue)

Tissue/organ Compound Time elapsed from the injection

1 h 3 h 6 h 9 h 12 h 24 h

Blood 188Re-NP 14.90 ± 0.20 10.40 ± 0.51 7.76 ± 0.25 4.42 ± 0.24 2.31 ± 0.20 1.32 ± 0.12

188Re 15.04 ± 0.12 9.40 ± 0.80 7.36 ± 0.20 5.00 ± 0.34 2.64 ± 0.48 0.94 ± 0.24

p >0 .2 p> 0.2 p> 0.1 p> 0.1 p> 0.2 p> 0.1

Liver 188Re-NP 5.22 ± 0.36 4.09 ± 0.13 3.69 ± 0.23 3.06 ± 0.25 1.02 ± 0.07 0.07 ± 0.01

188Re 5.44 ± 0.29 4.66 ± 0.42 3.53 ± 0.18 1.98 ± 0.51 1.36 ± 0.24 0.15 ± 0.05

p> 0.2 p> 0.2 p> 0.2 p> 0.1 p> 0.1 p> 0.1

Kidneys 188Re-NP 7.27 ± 0.42 5.04 ± 0.21 4.47 ± 0.29 3.32 ± 0.35 1.84 ± 0.19 0.35 ± 0.07

188Re 7.34 ± 0.46 5.35 ± 0.56 3.67 ± 0.35 2.08 ± 0.41 1.44 ± 0.25 0.35 ± 0.07

p> 0.8 p> 0.5 p> 0.1 p> 0.1 p> 0.1 p> 0.6

Lungs 188Re-NP 10.01 ± 0.50 7.46 ± 0.31 5.68 ± 0.16 4.61 ± 0.32 2.75 ± 0.23 0.34 ± 0.06

188Re 10.09 ± 0.50 7.79 ± 0.52 5.52 ± 0.26 3.31 ± 0.38 1.99 ± 0.33 0.30 ± 0.05

p> 0.5 p> 0.2 p> 0.3 p> 0.1 p> 0.1 p> 0.2

Spleen 188Re-NP 9.77 ± 1.17 7.66 ± 0.43 4.74 ± 0.35 4.15 ± 0.30 2.16 ± 0.06 0.29 ± 0.05

188Re 9.52 ± 0.40 6.90 ± 0.37 4.72 ± 0.47 3.01 ± 0.20 1.95 ± 0.33 0.31 ± 0.11

p> 0.8 p> 0.1 p> 0.8 p> 0.1 p> 0.2 p> 0.8

Femoral bone 188Re-NP 5.61 ± 5.61 3.77 ± 3.77 3.14 ± 3.14 2.13 ± 2.13 1.45 ± 1.45 0.44 ± 0.44

188Re 5.02 ± 0.29 3.98 ± 0.38 2.77 ± 0.21 1.58 ± 0.06 1.11 ± 0.09 0.44 ± 0.05

p> 0.1 p> 0.5 p> 0.1 p> 0.1 p> 0.1 p> 0.8

Tumor 188Re-NP 8.16 ± 0.26 8.20 ± 0.14 8.24 ± 0.06 6.54 ± 0.35 3.15 ± 0.35 1.17 ± 0.07

188Re 6.06 ± 0.17 3.82 ± 0.26 2.32 ± 0.42 1.90 ± 0.16 1.14 ± 0.09 0.66 ± 0.07

p< 0.03 p< 0.01 p< 0.01 p< 0.01 p< 0.05 p< 0.05

Time (h)

Fig. 2. Dynamics of 188Re-NP and 188Re accumulation (% of the Injected amount per 1 g of tissue) In the subcutaneously transplanted LLCImale In male C57Bl/6 mice

for the entire group. Lethal dendrimer doses caused transient motor excitation for the first 30 min that subsequently turned into sopor. The mice died within 144 hours after the injection depending on the dose of the dendrimer. A transient loss of weight (5-8%) and increased motor activity were observed in the surviving mice during the first 5 days following the injection. Necropsy revealed no visible signs of pathology in the heart, kidneys, spleen, and lungs of the animals; no visible pathology or hyperthermia were observed in the peritoneum; the liver was enlarged and its edges were blunt. The sublethal doses of the dendrimer did not induce any visible changes in the behavior or general health of mice: no ataxia or local paresis were observed. The mice were gaining weight at the rate of the control group. Their skin condition was normal. No macroscopic changes were noticed in the internal organs during autopsy. Based on the number of dead animals in each of 10 experimental groups, toxic dendrimer doses were calculated for mice: LD10 = 270 ± 92 mg/kg; LD16 = 382 ± 94 mg/kg; LD50 = 779 ± 111 mg/kg; LD84 = 1177 ± 196 mg/kg; LD90 = 1289 ± 260 mg/kg; LD100 = 1376 ± 367 mg/kg.

Table 1 compares the dynamics of 188RE-NP and 188Re-sodium perrhenate accumulation over time in mice with subcutaneously transplanted LLC1 carcinoma. The only significant difference was revealed for drug accumulation in the tumor, in contrast to healthy tissue. The dynamics of 188Re-NP and 188Re-sodium perrhenate accumulation in LLC1 are presented in Fig. 2

Because 188Re-NP is intended for anticancer radiation therapy, its tumor-to-normal uptake is an important pharmacokinetic characteristic. Calculated tumor-to-normal ratio (T/N) values for the most important organs are shown in Table 2. In the case of 188Re-NP, the T/N value was > 1 for almost all organs and tissues 3 h after the injection. This indicates more vigorous clearance of the substance from healthy organs than from the tumor. The rate of 188Re clearance from the tumor was comparable to that measured for the liver and the femoral bone.

The therapeutic efficacy of 188Re-NP was being studied for 30 days following tumor transplantation. A single injection

of the compound produced a marked tumoricidal effect (p < 0.05) throughout the entire observation period only at doses of 185 MBq/kg (Table 3, Fig. 3). Single doses of ^Re-sodium perrhenate taken at 185 MBq/kg had no therapeutic effect at all (Fig. 4). The T/C values for the studied 188Re doses are presented in Table 3.

The observed tumoricidal effect was dose-dependent. It was significant at a 188Re-NP dose of 185 MBq/kg, weak at 92.5 MBq/kg, and insignificant at 15 MBq/kg. The minimal effective therapeutic concentration of 188Re-NP was determined based on the curves demonstrating the dynamics of tumor growth.

DISCUSSION

This study shows that the toxicity of the proposed dendrimer is comparable to the toxicity of its analogs from the same class of compounds [31].

Our findings suggest that 188Re-NP taken up by the tumor is retained there for up to 6 hours following the injection. During this time period, the amount of 188Re-NP in the tumor remains high, making 8.2% of the injected amount. This may indicate the stability of the bonds between 188Re-NP and the tumor tissue. The dynamics of 188Re sodium perrhenate accumulation does not follow the same pattern. The maximum ratio of 188Re-NP to 188Re sodium perrhenate uptake by the tumor was 3.55 ± 0.660 6 hours after the injection.

The 188Re-NP dose of 185 MBq/kg at which the most significant tumoricidal effect was observed in mice was converted to a human dose equivalent of 15.42 MBq/kg. This is lower than the standard doses of radiopharmaceutical agents used in radiation therapy (44-47 MBq/kg) [32]. Consequently, the effective radiation dose can be reduced if 188Re-NP is used as a radiopharmaceutical agent. Another advantage of the proposed platform is its low toxicity. The studied substance was taken at 100-fold excess to ensure the complete binding of 188Re; the concentration of NP at 185 MBq/kg was

Table 2. Comparison of tumor-to-normal uptake ratio of 188Re-NP and 188Re by the organs and tissues of experimental mice

Tissue/organ Compound Time elapsed from the injection

1 h 3 h 6 h 9 h 12 h 24 h

Tumor/blood 188Re-NP 0.55 ± 0.02 0.79 ± 0.04 1.06 ± 0.04 1.48 ± 0.04 1.36 ± 0.06 0.89 ± 0.11

188Re 0.40 ± 0.01 0.41 ± 0.06 0.32 ± 0.07 0.38 ± 0.05 0.45 ± 0.11 0.72 ± 0.18

p> 0.1 p< 0.05 p< 0.004 p< 0.004 p< 0.004 p> 0.1

Tumor/liver 188Re-NP 1.57 ± 0.14 2.01 ± 0.09 2.24 ± 0.13 2.15 ± 0.24 3.09 ± 0.29 16.55 ± 2.7

188Re 1.11 ± 0.04 0.82 ± 0.02 0.66 ± 0.14 1.02 ± 0.37 0.86 ± 0.19 4.65 ± 1.25

p< 0.05 p< 0.004 p< 0.001 p< 0.004 p< 0.001 p< 0.001

Tumor/kidneys 188Re-NP 1.12 ± 0.07 1.63 ± 0.04 1.85 ± 0.11 1.99 ± 0.22 1.73 ± 0.30 3.42 ± 0.85

188Re 0.83 ± 0.07 0.72 ± 0.08 0.63 ± 0.09 0.95 ± 0.27 0.81 ± 0.19 1.91 ± 0.15

p< 0.004 p< 0.004 p< 0.004 p< 0.004 p< 0.05 p< 0.05

Tumor/lungs 188Re-NP 0.82 ± 0.06 1.10 ± 0.06 1.45 ± 0.05 1.43 ± 0.17 3.86 ± 0.80 13.92 ± 2.56

188Re 0.60 ± 0.04 0.49 ± 0.05 0.42 ± 0.09 0.58 ± 0.10 0.59 ± 0.14 2.18 ± 0.17

p< 0.05 p< 0 . 004 p< 0.004 p< 0.004 p< 0.02 p< 0.002

Tumor/spleen 188Re-NP 0.84 ± 0.11 1.07 ± 0.04 1.75 ± 0.12 1.58 ± 0.05 1.46 ± 0.12 4.09 ± 0.80

188Re 0.64 ± 0.04 0.55 ± 0.02 0.49 ± 0.04 0.63 ± 0.08 0.60 ± 0.14 2.26 ± 0.64

p< 0.05 p< 0.004 p< 0.004 p< 0.004 p< 0.004 p< 0.004

Tumor/femoral bone 188Re-NP 1.46 ± 0.10 2.17 ± 0.01 2.64 ± 0.23 3.08 ± 0.22 2.18 ± 0.32 2.67 ± 0.15

188Re 1.21 ± 0.10 0.97 ± 0.13 0.85 ± 0.21 1.20 ± 0.14 1.04 ± 0.14 1.51 ± 0.31

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p< 0.05 p< 0.004 p< 0.004 p< 0.004 p< 0.05 p< 0.004

Tumor 188Re-NP/Tumor 188Re 1.35 ± 0.05 2.15 ± 0.19 3.55 ± 0.66 3.45 ± 0.32 2.76 ± 0.51 1.79 ± 0.24

Table 3. The therapeutic effect of the 188Re-NP against the LLC1 carcinoma

Time elapsed from the Injection, days 7 days 14 days 18 days

The dose of 188Re, MBq/kg 15 92.5 185 15 92.5 185 15 92.5 185

Т/С, % 100 41 5 74 55 13 87 75 20

Note: The table shows the results of therapeutic efficacy assessment on days 7, 14 and 18 after drug administration.

А

B

15 000-

J= 10 000

5000

- Control

■ 188Re-NP (15 MBq/kg)

■ 188Re-NP (92.5 MBq/kg)

■ 188Re-NP (185 MBq/kg)

15 000

E 10 000 -

5000

Control

- 188Re-neppeHaT (185 MBq/kg) • 188Re-NP (185 MBq/kg)

0 5 10 15 20 25 30 Time elapsed from the 188Re-NP injection (days)

Fig. 3. The growth dynamics of the subcutaneously transplanted LLC1 tumor in C57Bl/6 mice following a single injection of 188Re-NP taken at different 188Re doses and a single injection of 188Re sodium perrhenate and 188Re -NP (188Re dose = 185 MBq/kg) (B)

4.05 • 10-3 mg/kg (or 2.16 • 10-3 mg/kg of the dendrimer), which is lower than LD10 by 5 orders of magnitude. Our findings suggest that the proposed NP may hold promise as a potent radiopharmaceutical.

CONCLUSIONS

This study demonstrates the feasibility of the proposed dendrimer-based platform for targeted drug delivery of tumoricidal agents. We have established the minimally effective therapeutic dose of 188Re in the studied compound

and revealed that the synthesized dendrimer exhibits dose-dependent activity against tumor cells. Acute toxicity tests conducted in mice have shown that the 188Re-NP platform is lowly toxic and ensures a considerable tumoricidal effect at doses much lower than LD10. The levels of 188Re-NP accumulation in the tumor, as well as the value of the T/N ratio, lead us to conclude that the compound can be safely used to enhance the therapeutic dose of p-radiation in the tumor. The 188Re component of the 188Re-NP composition taken at a dose of 185 Mbq/kg induces a marked tumoricidal effect 18 days following its administration.

0

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