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15THz-O-3
Detection of skin pathologies using THz spectroscopy
I. Yanina1'2, V. Nikolaev24, A. Borisov23, A. Knyazkova234, E. Buyko4, V. Kochubey12, V.
Ivanov5, Yu. Kistenev23, V. Tuchin126
1- Saratov State University (National Research University), Research-Educational Institute of Optics and Biophotonics, 83 Astrakhanskaya str., Saratov 410012, Russia 2- Tomsk State University (National Research University), Laboratory of laser molecular imaging and machine
learning, 36Lenin's av., Tomsk 634050, Russia 3- Siberian State Medical University, Department of Physics and Mathematics, 2 Moskovskytrakt, Tomsk
634055, Russia
4- Russia Institute of Strength Physics and Materials Science of SB RAS, Laboratory ofMolecular Imaging and
Photoacoustics, 2/4Akademicheskyave., Tomsk 634055, Russia
5-Siberian State Medical University, Laboratory of Biological Models, 2 Moskovskytrakt, Tomsk 634055,Russia
6- Institute of Precision Mechanics and Control RAS, Laboratory of Laser Diagnostics of Technical and Living
Systems, 24 Rabochaya, Saratov 410028, Russia
e-mail: irina-yanina@list.ru
Terahertz (THz)-based detection is non-ionizing and non-invasive, represents a very attractive tool foi repeated assessments, patient monitoring, and follow-up [1,2]. Because differences between healthy and unhealthy tissues can precisely differentiate between different types of molecules, depending on water content and the difference in the properties of adipose and muscle tissue and their structure [3,4], the information obtained through THz-based scanning could have several uses in the management of patients and, more importantly, in the early detection of different pathology[5-7]. The difficulty of interpreting measurements and the transition from these measurements to in vivo diagnostics is caused by various reasons, for example, diffusion into a sample of saline during storage, changes in the level of hydration during the measurement, effects of scattering [8]. The purpose of this study was to detect of skin pathologies using THz spectroscopy using optical clearing method. Studies were conducted on diabetes animal's model (the mouse and rat) in vitro and in vivo. The study of varying degrees of diabetes was considered as the main pathology. For measurements, each animal was leaned against the prism of the skin surfaceand several locations in the skin of each animal were analyzed. Places on the skin were chosen so that the intensity spectrum of the THz signal was the same for different points within the error. The measurements were carried out in reflection mode (prism of impaired total internal reflection-TIR from silicon). Various antireflection agents were applied to the skin of the animal at these points. Measurements are taken every 5 minutes for 60 minutes. It has been shown that glucose is promising for use as an optical clearing agent in the terahertz region. For in vitro measurements, skin samples of small animals stored in saline solution were used. These samples were placed on the surface of the TIR prism, and after measurement, they were placed in a cell with an optical clearing agent. Then, every 5 minutes for 60 minutes, the sample was taken out and a measurement was performed on the prism of the TIR. The findings of in vivo studies have been confirmed for in vitro studies.
The authors would like to acknowledge the support from RFBR grant No. 18-52-16025 (IYuY, VVN, VIK and VVT), and grant under the Decree of the Government of the Russian Federation No. 220 of 09 April 2010 (Agreement No. 075-15-2021-615 of 04 June 2021).
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