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24THz-PS-2
Determination of changes in the ratio of water / fat in adipose tissue when heated using terahertz technology
I. Yanina1,2, V. Nikolaev2,3, O. Zakharova2,3, A. Borisov2,4, V. Kochubey1,2, Y. Kistenev2,4, V. Tuchin125
1Saratov State University National Research University, Research-Educational Institute of Optics and Biophotonics, Saratov, Russian Federation
2Tomsk State University National Research University, Interdisciplinary Laboratory of Biophotonics, Tomsk, Russian Federation
3Institute of Strength Physics and Materials Science of SB RAS, Laboratory of Molecular Imaging and Photoacoustics, Tomsk, Russian Federation
4Siberian State Medical University, Department of Physics and Mathematics, Tomsk, Russian Federation
5Institute of Precision Mechanics and Control RAS, Laboratory of Laser Diagnostics of Technical and Living Systems, Saratov, Russian Federation
Terahertz (THz) spectroscopy allows one to determine the complex refractive index of the medium under study, which is important for creating a functional THz -tomograph with high sensitivity to changes in the concentration of metabolites and accurate marking of the boundaries of the pathological process. Therefore, the development of spectroscopic methods for studying biological tissues in the THz -frequency range, providing detection and visualization of metabolic and pathological processes, has caused great interest in recent years, especially as an additional channel for obtaining information in multimodal systems in combination with optical methods [1,2]. The contrast between healthy and diseased tissue in the THz region is due to both the change in water content [3-5] and the difference in the properties of adipose and muscle tissue and their structure [6-8]. The difficulty of interpreting measurements and the transition from these measurements to in vivo diagnostics is caused by various reasons, for example, diffusion into a sample of saline during storage, changes in the level of hydration during the measurement, effects of scattering [9].The purpose of this study was to study the effect of temperature change on the water / lipid ratio in adipose tissue in the terahertz range.
We used pig abdominal adipose tissue. The number of samples is 10. The thickness of the samples should be about 1 mm. Sample sizes were 1 cm2. The study of fat cells was carried out in vitro.
The temperature varied from 25°C to 70°C. A thermistor was usedto samples heating.The sample is clamped between two sheets of fluoroplastic, 2 x 1.5 cm in size. The thermistor was placed on the instrument stage.Measurements were performed using a real-time terahertz spectrometer (EKSPLA, Lithuania). The application "THzSpectrometer 2D" was used.
It was found the changes in the ratio of water / fat in adipose tissue when heated using terahertz technology.
The authors would like to acknowledge the support from RFBR grant No. 18-52-16025 (IYuY, VVN, VIK and VVT), the National Research Tomsk State University Academic D.I. Mendeleev Fund Program (IYuY, VVN, VIK, VVTandYuVK), Fundamental Research Program of the State Academies of Sciences for 2013-2020, line of research III.23.a (VVN).
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