CC BY
35B-I-33
Combatting bacterial biofilms and bacterial plankton for medicine and food industry via laser nanotechnology
A.A. Ionin1, S.A. Gonchukov12, S.I. Kudryashov13, A.A. Nastulyavichus13, Yu.M. Romanova4, I.N. Saraeva13, A. A. Semenova3, N.A. Smirnov1, E.R. Tolordava1,3 4, Yu. K. Yushina3
1-P. N. Lebedev Physical Institute, 53 Leninsky prospect, 119991, Moscow, Russia 2 - National Research Nuclear University MEPhI, 31 Kashirskoe shosse, 115409, Moscow, Russia 3 - V.M. Gorbatov Federal Research Center for Food Systems, 26 Talalikhina Str., 109316, Moscow, Russia 4 - N.F. Gamaleya Federal Research Center of Epidemiology and Microbiology, 25 Gamaleya Str., 123098, Moscow, Russia
aion@sci. lebedev. ru
Solving the problem of pathogenic bacteria resistance is a key challenge of modern medicine and food industry. Nowadays, controlling pathogenic microorganisms is actually a global issue. Active and passive hybrid ways developed by us for controlling and suppressing biofilms and the planktonic form of pathogenic microorganisms based on different bactericidal nanomaterials obtained with modern laser technologies are discussed, which can find applications in medicine and food industry. An innovative mobile laser application of the complete suppression of biofilms in situ is described: namely, the procedure of laser-induced forward transfer (LIFT) for inactivation of pathogenic bacterial biofilms by metallic nanoparticles (NPs). The transparent substrate coated with the thin absorbing metal layer is irradiated form the back side with a laser that causes the NPs be transferred due to lightmatter interaction (Fig.1, left). Variable laser beam parameters and the spacing between donor and the glass substrate affect parameters of the transferred NPs as well as their quantity.
Fig. 1. LIFT technology for transferring NPs onto a biofilm (left); schematic of the flow-along-nanostructures filter: (a) upper and (b)
stereometric view (right)
The laser-ablative fabrication of antibacterial nanostructures, combining mechanical and chemo-toxic effects, by femto- and nanosecond laser ablation and their testing on Staphylococcus aureus and Pseudomonas aeruginosa are discussed. A titanium surface covered with nanospikes and periodical surface ripples provides a strong anti-biofilm effect, and the addition of cytotoxic NPs (Ag, Se) enhances the overall bactericidal activities. Application of a flow reactor including Ti nanospike-covered wafers as a through-flow sterilizer (Fig.1, right) resulted in reduction of the bacterial population by two orders of magnitude. Food pathogens (Staphylococcus aureus, Listeria monocytogenes, and Pseudomonas aeruginosa) in planktonic form were subjected to bactericidal treatment by colloidal nickel-oxide NPs. These colloidal NPs, as anti-biotic nanomaterial, were produced by laser ablation in deionized water and air, and comprehensively characterized by x-ray diffraction, scanning electron microscopy, etc. The opportunity of a time-resolved bacterial live/dead dynamics observation with the use of plasmonic nanospikes was demonstrated. Sharp nanospikes, fabricated on a 500-nm thick gold film by laser ablation with the use of 1030-nm femtosecond pulses, were tested as potential elements for antibacterial surfaces and plasmonic luminescence sensors.
