Научная статья на тему 'Bioguided isolation of bioactive compounds from Sphaeranthus africanus'

Bioguided isolation of bioactive compounds from Sphaeranthus africanus Текст научной статьи по специальности «Фундаментальная медицина»

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Текст научной работы на тему «Bioguided isolation of bioactive compounds from Sphaeranthus africanus»

I Abstracts. PHYTOPHARM 2017


© Huyen Tran Thi, Olaf Kunert, San-Po Pan, Teresa Pirker, Nadine Kretschmer, Rudolf Bauer

Institute of Pharmaceutical Sciences, Department of Pharmacognosy, University of Graz, Graz, Austria

Sphaeranthus africanus has been widely used in popular medicine in Vietnam for antiseptic and antipyretic. It is distributed throughout the plains and wet-lands in central Vietnam.

The aim of this study was to investigate the cytotoxic and anti-inflammatory activity of Sphaeranthus africanus extracts, and finally to isolate the active compounds and to elucidate their structure.

The aerial parts of Sphaeranthus africanus were extracted with n-hexane, dichloromethane and ethyl acetate. The extracts were tested for cytotoxicity with various cancer cell lines, and for COX-2 expression in

THP1 monocytes. After activity guided fractionation, components were isolated by column chromatography. Their structures were elucidated by spectrometry such as UV, MS and NMR.

The n-hexane and dichloromethane extracts showed cytotoxic activity, why they were used to isolate the active components. Two compounds were identified as carvotanacetone derivatives. The isolation and structural elucidation of further compounds is in progress.

In Sphaeranthus africanus, we could identify compounds with cytotoxic and anti-inflammatory activity which may explain in part the traditional use of this plant.


© Tubon Irvin1, Bernardini Chiara1, Zannoni Augusta1, Vinueza Diego2, Abdo Susana2, Forni Monica1

1 Department of Veterinary Medical Sciences, University of Bologna, Bologna, Italy;

2 Escuela de Bioquimica y Farmacia, Facultad de Ciencias, Escuela Superior Politecnica de Chimborazo, Riobamba, Ecuador

Angiogenesis is defined as sprouting of new capillaries from pre-existing vasculature, and involves proliferation and migration of endothelial cells [1]. Primary culture of porcine Aortic Endothelial Cells (pAEC) are used in many in vitro models confirming swine as a relevant translational animal model [2]. Although in Ecuador about 80% of the population depends on the traditional medicine, there is a lack of scientific information that support the use of plant extracts [3]. The aim of the present research was to evaluate cytotoxic and angiogenic properties of ethanolic extracts prepared from the dried leaves of four Ecuadorian plant traditionally used to counteract inflammation, common cold, infection, healing or ulcers. pAEC were cultured in the presence of extracts (1-200 ug/ml) for 24 h, then cell viability was evaluated by MTT assay. Campyloneurum amphostenon (Kunze ex Klotzsch) Fée and Aristeguietia glutinosa (Kunth) R.M.King & H.Rob have decreased cellular viability at doses higher than 25 ug/ml. Clinopodium tomentosum (Kunth) Govaerts and Salvia quitensis Benth did not affect the cellular viability at any dose tested. In vitro pro-angiogenic activity of the two extracts that not presented cytotoxicity was tested by


scratch test. C. tomentosum increased cell migration by about 73% (25 ug/ml) after 24 hours, while the S. quitensis extract did not increased cell migration. Thereafter, the C. tomentosum extract was tested in a 3D assay to study its ability to induce the formation of a capillary-like tube structure. pAEC were plated on a layer of polymerized extracellular matrix. After 18 hours C. tomentosum induced a complete capillary like network with a dose dependent increase of master junction. In conclusion, we demonstrated "In vitro" the regulation of vascular cells dynamics by plants extracts with C. tomentosum showing clear pro-angiogenetic properties.


1. Carmeliet P, Jain RK. 2000. Nature. 407:249-257.

2. Botelho G, Bernardini C, Zannoni A, Ventrella V, Bacci ML, Forni M. 2015. Comparative Biochemistry and Physiology Part C. 176-177:79-86.

3. Buitrón CX. 1999. Ecuador: uso y comercio de plantas medicinales, situación actual y aspectos importantes para su conservación. TRAFFIC international.

Obzory po kliniceskoj farmacologii i lekarstvennoj terapii [Reviews of clinical pharmacology and drug therapy]

vol. 15/2Q17/suppLeMEnt 1

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