CC BY
21It was established experimentally that the cooling and thermal impact resistance reduces white rats to swim in all study days, especially 7 - 14 days. cold and heat reaction. When administered to experimental animals studied mixtures of RR and HP, increasing the voyage all the days of the experiment. So at 7 - 14 day experiment in rats after cold exposure the average length of the voyage was 1 40 + 7.2 min during thermal exposure - 1 5 0 + 5.2 min exposure controls during cold exposure-160 + 6.2 min; during thermal exposure - 180 + 5.2 min (p <0.01).
The study of resistance to physical stress in rats by trendbane the introduction of a mixture of PP and RFP under heat and cold exposure, showed that this impact reduces resistance to fatigue in rats. If m is the length of run on trendbane rats during cold exposure in the 7-14 day of the experiment was 7.9 + 0.5 min, during exposure to heat - 9.1 + 0.5 minutes while under the influence of rats studied product mixture during thermal exposure time increased to 18.5 + 2.1 min and cold exposure, 16.7 + 1.2 min respectively. Duration spine running intact rats during the experiment f virtually unchanged and was 12.2 + 2.0 min in the studied period. Conclusion
It was established experimentally that the duration cooling and thermal influence the activation of lipid peroxidation, as evidenced by the increase holding of lipid peroxidation products in the blood of rats. Introduction of a mixture of drugs RR and HP at a dose of 1 to 100 g weight of animals reduces the amount of peroxide oxidation of lipids in the blood of animals, thereby expediting the development of the second stage of adaptation and preventing exclusion.
When administered to rats studied mixture of drugs their RR and HP at a dose of 1 ml per 100 g um animal mass a period of prolonged cold and heat stress relief observed adaptation tion to cold and heat, as well as anti-oxidant found the mixture of RR and HP, manifested by decreased production of products Comrade lipid peroxidation in the blood of experimental animals.
Thus, we have proved effective correction cold and thermal stresses in the experimental use of a new mixture of drugs from the RR and HP. References
1. Archakov, AI Microsomal oxidation / A. Archakov. - Moscow: Nauka, 1975.-324 with.
2. Dorovskikh, VA Pharmacological correction of cold exposure in the experiment: Dis. ...Doctor. medical sciences.: 14.00.25 / Dorovskikh Vladimir A.;[Amursk. State. Honey. Academic.]. - Blagoveshchensk, 1987. - 290.
3. Kisilevich, GN Determination of vitamin E in the blood serum / RJ Kisilevich, SI Skvarko / / Laboratory business. - 1972. - № 8. - P. 473-475.
4. Korshunova, NV Toxicological and hygienic justification for the use of refined products antler to increase resistance to cold: dis ....Doctor. medical sciences.: 14.00.07 / Natalia Korshunova, [Amursk. State. Honey. Academic.]. - St. Petersburg., 2001.-224 with.
5. Panin, LE The role of nutrition in the change of energy metabolism under conditions of stress / L. Panin, DI Kuzmenko / / Issues. Supply. - 1982. - № 3. - Pp. 15-18.
6. Steel, ID Methods for determination of diene oh konyugation of higher unsaturated fatty acids / I.D Steel / / Modern Methods in Biochemistry / red. VN Orekhovich. - Moscow, Medicine, 1977. - P. 63-64.
Antioxidant properties of parietin
DorovskikhV. A.,DorovskikhV.Yu.
SBEE HPE "Amur State medical academy", department of pharmacology,Blagoveschensk, Russia
Parietin is a drug of vegetable origin. It was extracted from the lichen by B. F. Kerimov in 1982. The model of cold stress effect was chosen to determine antioxidant properties of parietin.
22
Cold effect was an everyday animals' keeping in climatic camera "Fentron" during 3 hours in the conditions of temperature - 15 degrees below zero and ventilation of camera.
Parietin is anthraquinon derivative. It is yellow powder with molecular weight 284, its fusion temperature is 206-207°,it is not soluble in water (V.A. Dorovskikh 1986).
Cold effect results in sharp increase of content of dien conjugates in the liver as well as in the lungs of experimental animals. It becomes appevent in the first 14 days of the experiment very distinctly. In 14 days of cold effect content of dien conjugates in the liver 1,9times and in the lungs 1,3 times was higher than in intact rats. Intact rats had lungs with index 0,1623±0,0002, cold rats had lungs with index 0,3091±0,011. Prior introduction of parietin resulted in decrease of content of dien conjugates in the rats' lungs (intact rats - 0,1623, cold rats - 0,3091, cold + parietin rats -0,2481).
Cold effect oppresses antioxidant system (Ye. Ye.Burlakova, 1975).
The content of tocopherol in homogenate of the lungs in the experiments in intact group made up 0,11±0,03 mrg per 1 mg of lipids. The content of tocopherol made up - 0,03±0,006 mkg per litre in rats subjected to cold effect to the 7 thday of cold. The content of tocopherol during introduction of parietin made up 0,08±0,01to the 7 thday of cold and 0,1±0,01 to the 14 thday of cold.
Thus,parietin prevented from sharp supression of antioxidant activiti of cold effect to the 7 thand 14 thdays of experiments.
Metabolic activiti of erythocytes, factors of molecular allosteric regulation of oxygenation (deoxegenation) of hemoglobin (V. A. Dorovskikh, Ye. A. Borodin, 2001, 2004, 2006) play the important in the improvement of oxygen regimen of an organism. Metabolite of phosphoglycerate shunt of glycolysis - 2,3biphosphoglyceric acid is allosteric regulator of oxygenation (deoxygenation of hemoglobin M. Peruts, 1965). The increase of the content of 2,3 BPG in erythvocytes must promote deoxygenation.
Cold effect decreasesthe content of 2,3 BPG in erythvocytes that contributes to the better oxygenation of hemoglobin in the lungs. However at the same time it limits sharply the return of oxygen to the tissues that contributes to their hypoxia. The content of 2,3 BPG in the intact rats is 18,6±1,6 to the 7 thday of cold is 12,4±1,2.
Parietin increased the content of 2,3 BPG in erythrocytes of rats subject to the cold effect (index - 16,5±1,6) - that results in return of oxygen to the peripherictissuses and reduced the level of hypoxia of tissuses.
Thus, the results received allow to come to the conclusion about the pronounced antioxidant and antihypoxant action of parietin.
References:
1. BurlacovaYe.Ye., Alexeenko A.V., Molochkina V.M. Bioantioxidants in the radiation damage and malignant growth. M. Nauka. 1975 - P. 214.
2. Dorovskikh V.A., Korshunova N.V., Krasavina N.P., Simonovfa N.V., Tikhanov V.I., Simonova N.P. Adaptogens and cold stress:yestedey, today, tomorrow... Blagoveschensk, 2006.
3. Dorovskikh V.A., Borodin Ye. A., Tseluyko S.S. Antioxidant in the prophylaxis and correction of cold streess. Blagovesehenssk, 2001.
4. Dorovskikh V.A., Tseluyko S.S. Antioxidant drugs of different chemical groups in regulation of stress effects. Blagovesehenssk, 2004.
5. Borodin Ye. A., Borodin G. P., Dorovskikh V.A., Doroshenko G. K., Yegorov K. Ye., Zrazhevskaya Ye. V. Kindeeva S. G. Biological membranes: Journal of membranous and cellular biology. 1992. T. 9. № 2. P. 622-628.
6. Kerimov B.F. The role of thiols exchange in the mechanism of adaptation during the influence of extreme factors on the organism. Baku. - 1982. Abstract of thesis, 17 P.
